| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 4,257,309 | C→T | 100% | intergenic (‑238/‑44) | CJ019_03991 ← / → tsaM1 | putative ABC transporter ATP‑binding protein/Toluene‑4‑sulfonate monooxygenase systemiron‑sulfur subunit TsaM1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 4,257,309 | 0 | C | T | 92.4% | 119.5 / 0.0 | 39 | intergenic (‑238/‑44) | CJ019_03991/tsaM1 | putative ABC transporter ATP‑binding protein/Toluene‑4‑sulfonate monooxygenase systemiron‑sulfur subunit TsaM1 |
| Reads supporting (aligned to +/- strand): ref base C (1/2); new base T (12/24); total (13/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
CGACGTGCCGCTGCAGCCGCCCCCTCGAATCGCAGAGGGCGCCCCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTGGTACGTCGCCTGCACCCCCGATGAGATCGCCACCAAACCCCTGGGCCGGCAGATCTGCGGGGAAAAA > KT2440/4257179‑4257439 | cgacgTGCCGCTGCAGCCGCCCCCTCGAATCGCAGAGGGCGCCCCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTaa < 2:346034/135‑1 (MQ=255)cgacgTGCCGCTGCAGCCGCCCCCTCGAATCGCAGAGGGCGCCCAATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTaa < 2:159193/135‑1 (MQ=255) acgTGCCGCTGCAGCCGCCCCCTCGAATCGCAGAGGGCGCCCCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAAt > 6:24750/1‑134 (MQ=255) cgccgcCTCGAATCGCAGAGGGCGCCCCATCAATGGATCCACTAAAAACCGATAAGCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAATGGATCCATtaaataaa < 4:116547/135‑1 (MQ=255) cccccTCGAATCGCAGAGGGCGCCCCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAATGGATCCATTAAATaaaaa < 6:372961/135‑1 (MQ=255) aaTCGCAGAGGGCGCCCCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAAcc < 4:309231/134‑1 (MQ=255) aTCGCAGAGGGCGCCCCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCg < 2:13795/135‑1 (MQ=255) tCGCAGAGGGCGCCCCATCAATGGATCCACTAAAAACCTATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCg < 3:122567/134‑1 (MQ=255) gCAGCGGGCGCCCCATCAATGGATCCACTAAAAACCGAAAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGgag < 5:281836/135‑1 (MQ=255) gCAGAGGGCGCCCCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGgag < 7:293389/135‑1 (MQ=255) cAGATGGCGCCCCATCAATGGATCCACTAAAAACCGATAATCGCTCAGAATCCGCACCTACCGAAGAAATTGCATTGACCTACCACGCCGACCGCAGCTTTAATGGATCCTTTAAATAAAAACAAAACCCGgag < 6:432076/134‑1 (MQ=255) aTCAATGGATCCACTAAAAACCGATAATCGCACCGAATCCGCACGTACCCAAAAAAATGCATTCACCTCCCACGCCGACCGCAGCCTTATTGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTcccgcggcc < 6:133004/135‑7 (MQ=255) aaTGGAACCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTAcccc < 1:81448/135‑1 (MQ=255) ccACAAAAAACCGATAATCGCACAGAATCAGCACCTACGGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAACACCCGGAGAGCCTCCCATGTACCCCAAAAaca < 4:44241/135‑1 (MQ=255) aCTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTaa > 1:436663/1‑78 (MQ=255) aCTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTaa < 2:436663/78‑1 (MQ=255) aCTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAGTGGATCCATTAAATAAAAACAAAACCCg > 8:22777/1‑106 (MQ=255) aCTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAGTGGATCCATTAAATAAAAACAAAACCCg < 7:15979/106‑1 (MQ=255) aCTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAGTGGATCCATTAAATAAAAACAAAACCCg < 7:22777/106‑1 (MQ=255) aCTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAGTGGATCCATTAAATAAAAACAAAACCCg > 8:15979/1‑106 (MQ=255) aaaaaCCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTGGt < 7:352930/136‑1 (MQ=255) aaaCCGATAATCGCACAGAATCCGCACCTACCCAACAAAATGCATTGACCTACCACGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTgg < 4:233490/133‑1 (MQ=255) gATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTGGTACGTCGc < 7:34756/136‑1 (MQ=255) aTAATCGCACAGAATCCGCACCTACAGAACAAAATGCATTGACCTACCACGGCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTGGTACGTCGc < 3:419420/135‑1 (MQ=255) aTCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACATACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTGGTACGTCGCCTg > 8:77418/1‑135 (MQ=255) ccACCTACCCAACAAAATCCATTGCCCTACCACGCCCACCCCACCTTTAATGCATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTGGTACGTCGCCTGCACCCCCGATgaga < 5:352296/134‑1 (MQ=255) ccGAACAAAATGCATTCACCTACCACGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTGGTACGTCGCCTGCACCCCCGATGAGATCGcca < 7:246358/134‑1 (MQ=255) cGAAAATAATGCATTGACCTACCAGCCCGACGGCAGCTTTAATTGATCCATAAAATAAAAACAAAACCCGGAGAGCCTCCGATGTACCCAAAAAACACCTGGTACGTCGCATGCACCCCCGATGAGATCGcca < 5:130882/133‑1 (MQ=255) cGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTGGTACGTCGc > 7:320356/1‑86 (MQ=255) cGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTGGTACGTCGc < 8:320356/86‑1 (MQ=255) cGCCGACCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTGGTACGTCGCCTGCACCCCCGATGAGATCGCCACCAAACCCCTGGGCCGGCAGATCTGc > 1:302259/1‑135 (MQ=255) aCCGCAGCTTTAATGGATCCATTAAATAAAAACAAACCCCGGAGAGCCTCCCATGTACCCCAAACACACCTGTTACGTCGCCTGCCCCCCCGATGAGAGCGCCCCCCCACCCCTGGGCCGGCAGCTCTGAgggg > 2:176038/1‑134 (MQ=255) aCCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTGGTACGTCGCCTGCACCCCCGATGAGATCGCCACCAAACCCCTGGGCCGGCAGATCTGCgggg > 2:116686/1‑134 (MQ=255) aCCGCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTGCTACGTCGCCTGCACCCCCGATGAGATCGCCACCAAACCCCTGGGCCGGCAGATCTGCgggg > 4:118871/1‑134 (MQ=255) gCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTGGTACGTCGCCTGCACCCCCGATga > 2:268397/1‑93 (MQ=255) gCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTGGTACGTCGCCTGCACCCCCGATga < 1:268397/93‑1 (MQ=255) gCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTGGTACGTCGCCTGCACCCCCGATGAGATCGCCACCAAACCCCTGGGCCGGCAGATCTGCGGGGaaa > 6:152271/1‑134 (MQ=255) gCAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTGGTACGTCGCCTGCACCCCCGATGAGATCGCCACCAAACCCCTGGGCCGGCAGATCTGCGGGGaaa > 4:224980/1‑134 (MQ=255) cAGCTTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTGGTACGTCGCCTGCACCCCCGATGAGATCGCCACCAAACCCCTGGGCCGGCAGATCTGCGGGGaaaaa < 4:413470/135‑1 (MQ=255) | CGACGTGCCGCTGCAGCCGCCCCCTCGAATCGCAGAGGGCGCCCCATCAATGGATCCACTAAAAACCGATAATCGCACAGAATCCGCACCTACCGAACAAAATGCATTGACCTACCACGCCGACCGCAGCCTTAATGGATCCATTAAATAAAAACAAAACCCGGAGAGCCTCCCATGTACCCCAAAAACACCTGGTACGTCGCCTGCACCCCCGATGAGATCGCCACCAAACCCCTGGGCCGGCAGATCTGCGGGGAAAAA > KT2440/4257179‑4257439 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |