Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	KT2440	5,757,239	(A)6→5	coding (781/834 nt)	CJ019_05400 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	5,757,234	0	A	.	92.9%	232.0 / 9.5	56	coding (776/834 nt)	CJ019_05400	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base . (32/20);  minor base T (0/4);  total (32/24)
Fisher's exact test for biased strand distribution p-value = 2.89e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGGAAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGCG  >  KT2440/5757116‑5757363                                                                                                                        |                                                                                                                                  gAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGa                                                                                                                >  4:101097/1‑137 (MQ=255)   aCAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCCCGCAAGAAGGCCCTCCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCACCAGGAAg                                                                                                              >  6:61462/1‑137 (MQ=255)       gACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCt                                                                                                          <  6:239764/137‑1 (MQ=255)          cAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgc                                                                                                       >  4:405726/1‑137 (MQ=255)               cGACCTGGCCAAGGTCTTGAAGACCATAGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAAAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgccgccc                                                                                                  >  1:157513/1‑136 (MQ=255)                    tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCcgccgccgcg                                                                                             >  5:189428/1‑136 (MQ=255)                    tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCAGCCAGCAGGAAGCGCTGGCCGCCGCCGCt                                                                                             >  5:181932/1‑137 (MQ=255)                    tGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCACGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGCAGCGCTGGCcgccgccgcg                                                                                             >  6:302996/1‑136 (MQ=255)                         aaGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAACCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCcg                                                                                        >  3:92001/1‑137 (MQ=255)                           ttttttGAAGACCATCGAGTAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCcgcg                                                                                      <  7:267150/133‑1 (MQ=255)                              cTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGAcc                                                                                   >  4:226007/1‑137 (MQ=255)                                  aaGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGc                                                                               <  7:317131/137‑1 (MQ=255)                                  aaGACCATCGAGCAACCCCTCGCCCGCCCGGCCGGTGAAGCTGAAGAGCCACGCAACACGGCCCTGCGGGCGCAGCA‑GCAAGC‑GTAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGc                                                                               <  4:3972/137‑1 (MQ=255)                                   aGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGcc                                                                              >  7:215388/1‑137 (MQ=255)                                   aGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGcc                                                                              >  1:375324/1‑137 (MQ=255)                                     cccATCGCGCAACCCCGCGCCCGCCCGGCCCGGGACGCGGACGCGCCACGCAAGACGCCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCg                                                                            <  4:82966/136‑1 (MQ=255)                                     aCCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCg                                                                            <  1:271820/137‑1 (MQ=255)                                     aCCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCg                                                                            <  4:127352/137‑1 (MQ=255)                                          cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGATCGGGCCCGTGAGc                                                                       >  7:72470/1‑137 (MQ=255)                                          cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGc                                                                       >  1:123793/1‑137 (MQ=255)                                          cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGc                                                                       >  7:52701/1‑137 (MQ=255)                                          cGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGc                                                                       >  3:4780/1‑137 (MQ=255)                                            aGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑ACAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGACt                                                                     >  6:75738/1‑137 (MQ=255)                                            aGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGccg                                                                     >  4:352862/1‑136 (MQ=255)                                                    cTCGCCCGCCAGGCCCGTGACGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGcc                                                             >  2:218344/1‑137 (MQ=255)                                                               ggCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAAccgc                                                  >  8:316786/1‑137 (MQ=255)                                                                 cccGTGATGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GTAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAAccgccg                                                <  8:8441/137‑1 (MQ=255)                                                                  ccGTGAAGCGGACGCGGCACGCAAGAAGGCCCTGCGGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGa                                               <  6:31188/137‑1 (MQ=255)                                                                        aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGCCCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAgg                                         >  8:173654/1‑137 (MQ=255)                                                                        aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAgg                                         >  1:113994/1‑137 (MQ=255)                                                                        aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAgg                                         >  8:425759/1‑137 (MQ=255)                                                                        aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAgg                                         >  5:53866/1‑137 (MQ=255)                                                                        aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAgg                                         >  7:113137/1‑137 (MQ=255)                                                                            taaGCGGCACGAAAGACGGCCCTGCTGGCGCAGCA‑GTAAGC‑GTAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCg                                     <  3:35071/136‑1 (MQ=255)                                                                            gAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCg                                     <  8:113137/137‑1 (MQ=255)                                                                            gAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCg                                     <  3:405726/137‑1 (MQ=255)                                                                            caaGAGGCACTCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCg                                     <  8:229178/136‑1 (MQ=255)                                                                            caaGAGGCACGAAAGAAGGCCCTGATGGCGCAGAA‑GGAAGCGG‑AAAACCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCg                                     <  3:121445/136‑1 (MQ=255)                                                                            aatgagGCCCGCAAGAAGGCCCGGCTGGCG‑ACCACGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCAGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCg                                     <  2:461346/134‑1 (MQ=255)                                                                                agGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTacc                                 >  7:213948/1‑137 (MQ=255)                                                                                  gCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGATCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTaccac                               <  8:72470/137‑1 (MQ=255)                                                                                  ccACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCTCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTaccac                               <  7:263440/136‑1 (MQ=255)                                                                                     cGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCAGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTAccccccg                            >  4:103453/1‑136 (MQ=255)                                                                                      gCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCcagc                                                                                                                    <  6:42774/48‑1 (MQ=255)                                                                                      gCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCcagc                                                                                                                    >  5:42774/1‑48 (MQ=255)                                                                                       cacgaagGCCAGGCGGGCGCAGCT‑GCAAGC‑GTAAAACCGCCGCCAGCAGGAAGCGCGGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGAAACCGCCGAAAAAGGCCCGTACCACCCTCg                          <  7:167613/134‑1 (MQ=255)                                                                                       cAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCg                          >  8:345676/1‑137 (MQ=255)                                                                                         agaacGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCggg                        <  7:280485/137‑1 (MQ=255)                                                                                          caagGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGc                       <  3:211778/136‑1 (MQ=255)                                                                                          caagGCCCTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGc                       <  8:156488/136‑1 (MQ=255)                                                                                               cccTGCTGGCGCAGCA‑GGAAGC‑GTAAAAACGCCGCCAGCAGGACGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATg                  <  2:322917/137‑1 (MQ=255)                                                                                                ccTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGaa                                                                                                               <  5:137623/43‑1 (MQ=255)                                                                                                ccTGCTGGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGaa                                                                                                               >  6:137623/1‑43 (MQ=255)                                                                                                   gctgGCGCAGCA‑GGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGttt              >  8:357373/1‑137 (MQ=255)                                                                                                           agca‑gGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGAt      >  6:30745/1‑137 (MQ=255)                                                                                                                ggAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTCCCACCCTCGGGCCGATGGTTTCCAGCGATGgcg  >  4:98637/1‑137 (MQ=255)                                                                                                                        |                                                                                                                                  GAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCGTGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCA‑GGAAGCGGAAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTTCCAGCGATGGCG  >  KT2440/5757116‑5757363

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑