Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	KT2440	3,779,014	(C)6→7	intergenic (+6/+10)	CJ019_03590 → / ← mhpT	hypothetical protein/3‑(3‑hydroxy‑phenyl)propionate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	3,779,008	1	.	C	96.9%	224.6 / 0.3	64	coding (1146/1146 nt)	CJ019_03590	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (2/0);  new base C (30/32);  total (32/32)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01

GAAGGCCCCTGCCACTGAGCTCGGCGCCTTGCTCAGCAGTGCAGACAGCGCCATGTACCTTTCCAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑G‑CCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCGGGTTCGGTGTTTCAGTAGCGCACGTGCCGCCAACGCCGCGAGCACCAGGCCTGGAGCCGCGG  >  KT2440/3778876‑3779136                                                                                                                                       |                                                                                                                                  gAAGGCCCCTGCCACTGAGCTCGGCGCCTTGCTCAGCAGTGCAGACAGCGCCATGTACCTTTCCAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGCGCAGGAGGCATTGCGCAAGCAGCACTA‑G‑ccc‑c                                                                                                                              >  3:377176/1‑137 (MQ=255) gAAGGCCCCTGCCACTGAGCTCGGCGCCTTGCTCAGCAGTGCAGACAGCGCCATGTACCTTTCCAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑G‑ccc‑c                                                                                                                              >  1:116576/1‑137 (MQ=255) gAAGGCCCCTGCCACTGAGCTCGGCGCCTTGCTCAGCAGTGCAGACAGCGCCATGTACCTTTCCAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑G‑ccc‑c                                                                                                                              >  1:91342/1‑137 (MQ=255)  aaGGCCCCTGCCACTGAGCTCGGCGCCTTGCTCAGCAGTGCAGACAGCGCCATGTACCTTTCCAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑G‑ccc‑cc                                                                                                                             <  8:134447/137‑1 (MQ=255)     gCCCCTGCCACTGAGCTCGGCGCCTTGCTCAGCAGTGCAGACAGCGCCATGTACCTTTCCAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑G‑ccc‑cccca                                                                                                                          <  1:288225/137‑3 (MQ=255)       cccTGCCACTGAGCTCGGCGCCTTGCTCAGCAGTGCAGACAGCGCCATGTACCTTTCCAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCAAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑G‑ccc‑ccccaag                                                                                                                        <  4:105156/137‑5 (MQ=255)          tGCCACTGAGCTCGGCGCCTTGCTCAGCAGTGCAGACAGCGCCATGTACCTTTCCAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGaa                                                                                                                      <  6:448224/137‑1 (MQ=255)          tGCCACTGAGCTCGGCGCCTTGCTCAGCAGTGCAGACAGCGCCATGTACCTTTCCAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGaa                                                                                                                      <  5:281935/137‑1 (MQ=255)                 gAGCTCGGCGCCTTGCTCAGCAGTGCAGACAGCGCCATGTACCTTTCCAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTAt                                                                                                               <  7:427638/137‑1 (MQ=255)                 gAGCTCGGCGCCTTGCTCAGCAGTGCAGACAGCGCCATGTACCTTTCCAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTAt                                                                                                               <  7:340883/137‑1 (MQ=255)                 gAGCTCGGCGCCTTGCTCAGCAGTGCAGACAGCGCCATGTACCTTTCCAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTAt                                                                                                               <  6:170250/137‑1 (MQ=255)                 gAGCTCGGCGCCTTGCTCAGCAGTGCAGACAGCGCCATGTACCTTTCCAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTAt                                                                                                               <  4:400039/137‑1 (MQ=255)                 gAGCTCGGCGCCTTGCTCAGCAGTGCAGACAGCGCCATGTACCTTTCCAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTAt                                                                                                               <  2:349597/137‑1 (MQ=255)                 gAGCTCGGCGCCTTGCTCAGCAGTGCAGACAGCGCCATGTACCTTTCCAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTAt                                                                                                               <  2:347445/137‑1 (MQ=255)                       ggCGCCTTGCTCAGCAGTGCAGACAGCGCCATGTACCTTTCCAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCGCACCGGAGCAGGAGGCATTGCGCCAGCAGCCCTC‑GCCCC‑CCCAAGGAAGTGCTATGCAggg                                                                                                         >  8:440574/1‑137 (MQ=255)                          gcCTTGCTCAGCAGTGCAGACAGCGCCATGTACCTTTCCAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTcc                                                                                                      >  4:133893/1‑137 (MQ=255)                            cTTGCTCAGCAGTGCAGACAGCGCCATGTACCTTTCCAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTg                                                                                                    >  7:333696/1‑137 (MQ=255)                              tGCTCAGCAGTGCAGACAGCGCCATGTACCTTTCCAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTggg                                                                                                  <  6:314273/137‑1 (MQ=255)                              tGCTCAGCAGTGCAGACAGCGCCATGTACCTTTCCAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTggg                                                                                                  <  1:180009/137‑1 (MQ=255)                                   agcagTGCAGACAGCGCCATGTACCTTTCCAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCa                                                                                             <  8:39999/137‑1 (MQ=255)                                      agTGCAGACAGCGCCATGTACCTTTCCAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGcc                                                                                          >  2:58694/1‑137 (MQ=255)                                        tGCAGACAGCGCCATGTACCTTTCCAATGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTc                                                                                        <  6:202157/137‑1 (MQ=255)                                           aGACAGCGCCATGTACCTTTCCAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCAcc                                                                                     >  7:275830/1‑137 (MQ=255)                                             aCAGCGCCATGTACCTTTCCAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑G‑CCCACCCAAGGAAGTGATATGCAGGTGCCTGGGCTGCAGCCTCACCCg                                                                                   >  4:397564/1‑137 (MQ=255)                                                     aTGTACCTTTCCAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTAt                                                                                                               <  6:278396/101‑1 (MQ=255)                                                     aTGTACCTTTCCAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTAt                                                                                                               >  5:278396/1‑101 (MQ=255)                                                     aTGTACCTTTCCAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCCCCCGGCGCCggg                                                                           >  5:65490/1‑137 (MQ=255)                                                     aTGTACCTTTCCAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCggg                                                                           >  2:53023/1‑137 (MQ=255)                                                     aTGTACCTTTCCAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCggg                                                                           >  5:248308/1‑137 (MQ=255)                                                                cAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGt                                                                                                        >  1:411705/1‑97 (MQ=255)                                                                cAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGt                                                                                                        <  2:411705/97‑1 (MQ=255)                                                                cAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTtgcgtgcg                                                                >  1:256110/1‑137 (MQ=255)                                                                 aaGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTAAGCAGGGTCCTGGGCGGCAGACTCACCCGGCTCCGGGCGTTCCGTGCgg                                                               >  2:193313/1‑137 (MQ=255)                                                                  aGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCggg                                                              <  8:3393/137‑1 (MQ=255)                                                                  aGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCggg                                                              <  2:91342/137‑1 (MQ=255)                                                                  aGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCggg                                                              <  3:133893/137‑1 (MQ=255)                                                                  aGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCggg                                                              <  3:153450/137‑1 (MQ=255)                                                                  aGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCggg                                                              <  1:193313/137‑1 (MQ=255)                                                                    gTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCCAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCGGGtt                                                            <  4:239034/137‑1 (MQ=255)                                                                    gTATCGGGCCGTAACCGGGGCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCGGGtt                                                            <  2:145180/137‑1 (MQ=255)                                                                        cGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCGGGTTCGgt                                                        >  3:180731/1‑137 (MQ=255)                                                                          ggCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCGGGTTCGgtgt                                                      >  2:300554/1‑137 (MQ=255)                                                                          ggCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCGGGTTCGgtgt                                                      >  1:24558/1‑137 (MQ=255)                                                                           gCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTAt                                                                                                               <  3:241174/79‑1 (MQ=255)                                                                           gCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTAt                                                                                                               >  4:241174/1‑79 (MQ=255)                                                                           gCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCGGGTTCGGTGtt                                                     >  3:228921/1‑137 (MQ=255)                                                                           gCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCGGGTTCGGTGtt                                                     >  4:54466/1‑137 (MQ=255)                                                                           gCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCGGGTTCGGTGtt                                                     >  8:379657/1‑137 (MQ=255)                                                                                  ccGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCGGGTTCGGTGTTTCAGTAg                                              >  8:338871/1‑137 (MQ=255)                                                                                  ccGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCGGGTTCGGTGTTTCAGTAg                                              >  1:114188/1‑137 (MQ=255)                                                                                  ccGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCGGGTTCGGTGTTTCAGTAg                                              >  5:60786/1‑137 (MQ=255)                                                                                  ccGGGTCACGGTCGCCGAACCGGAGCAGGAGGAATTGCGCAAGCAGCACTATC‑CCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCTTGCGGGTTCGGTGTTTCAGTAg                                              >  6:411394/1‑137 (MQ=255)                                                                                   cGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCGGGTTCGGTGTTTCAGTAgc                                             <  5:394162/137‑1 (MQ=255)                                                                                      gTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCGGGTTCGGTGTTTCAGTAGCGCa                                          <  5:396683/137‑1 (MQ=255)                                                                                        cACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCGGGTTCGGTGTTTCAGTAGCGCACg                                        >  5:145917/1‑137 (MQ=255)                                                                                        cACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGAAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCGGGTTCGGTGTTTCAGTAGCGCACg                                        >  7:365003/1‑137 (MQ=255)                                                                                                   aaCCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCGGGTTCGGTGTTTc                                                   >  6:380076/1‑115 (MQ=255)                                                                                                   aaCCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCGGGTTCGGTGTTTc                                                   <  5:380076/115‑1 (MQ=255)                                                                                                   aaCCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCGGGTTCGGTGTTTCAGTAGCGCACGTGCCGCCAAcg                             >  7:276457/1‑137 (MQ=255)                                                                                                                      gcgcAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCGGGTTCGGTGTTTCAGTAGCGCACGTGCCGCCAACGCCGCGAGCACCAGGCCTgg          >  6:202570/1‑137 (MQ=255)                                                                                                                      gcgcAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCGGGTTCGGTGTTTCAGTAGCGCACGTGCCGCCAACGCCGCGAGCACCAGGCCTgg          >  1:45896/1‑137 (MQ=255)                                                                                                                        gcAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCGGGTTCGGTGTTTCAGTAGCGCACGTGCCGCCAACGCCGCGAGCACCAGGCCTGGAg        <  6:60786/137‑1 (MQ=255)                                                                                                                        gcAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCGGGTTCGGTGTTTCAGTAGCGCACGTGCCGCCAACGCCGCGAGCACCAGGCCTGGAg        <  7:338871/137‑1 (MQ=255)                                                                                                                        gcAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCGGGTTCGGTGTTTCAGTAGCGCACGTGCCGCCAACGCCGCGAGCACCAGGCCTGGAg        >  1:162590/1‑137 (MQ=255)                                                                                                                        gcAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCGGGTTCGGTGTTTCAGTAGCGCACGTGCCGCCAACGCCGCGAGCACCAGGCCTGGAg        <  8:276457/137‑1 (MQ=255)                                                                                                                        gcAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCGGGTTCGGTGTTTCAGTAGCGCACGTGCCGCCAACGCCGCGAGCACCAGGCCTGGAg        <  1:4597/137‑1 (MQ=255)                                                                                                                         cAAGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCGGGTTCGGTGTTTCAGTAGCGCACGTGCCGCCAACGCCGCGAGCACCAGGCCTGGAGc       >  4:309872/1‑137 (MQ=255)                                                                                                                          acgcagcaCTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCGGGTTCGGTGTTTCAGTAGCGCACGTGCCGCCAACGCCGCGAGCACCAGGCCTGGAGcc      <  6:462195/135‑1 (MQ=255)                                                                                                                          aaGCAGCACTA‑GCCCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCGGGTTCGGTGTTTCAGTAGCGCACGTGCCGCCAACGCCGCGAGCACCAGGCCTGGAGcc      <  8:333696/137‑1 (MQ=255)                                                                                                                              agcaCTA‑GCCCC‑CCCAACGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCGGGTTCGGTGTTTCAGTAGCGCACGTGCCGCCAACGCCGCGAGCACCAGGCCTGGAGCCGCgg  <  2:116576/137‑1 (MQ=255)                                                                                                                                       |                                                                                                                                  GAAGGCCCCTGCCACTGAGCTCGGCGCCTTGCTCAGCAGTGCAGACAGCGCCATGTACCTTTCCAAGGTATCGGGCCGTAACCGGGTCACGGTCGCCGAACCGGAGCAGGAGGCATTGCGCAAGCAGCACTA‑G‑CCC‑CCCAAGGAAGTGCTATGCAGGGTCCTGGGCTGCAGCCTCACCCGGCTCCGGGCGTTGCGTGCGGGTTCGGTGTTTCAGTAGCGCACGTGCCGCCAACGCCGCGAGCACCAGGCCTGGAGCCGCGG  >  KT2440/3778876‑3779136

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑