| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 1,935,418 | 0 | C | A | 59.1% | ‑1.8 / 19.8 | 22 | intergenic (+39/‑166) | CJ019_01850/yjcH_1 | hypothetical protein/Inner membrane protein YjcH |
| Reads supporting (aligned to +/- strand): ref base C (6/3); new base A (13/0); total (19/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.45e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.75e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGATCCAGGAAGGGGCCAAGCCTGAAGCGGCCGCCAAGGACTGGGTCGCCAAGCACCCTGATCGTGTGAAGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTAAACCCGCTCCTACGGGTGCGGGTTTACCCGCGAACGGGTCATCACTTTGTTACGCAATCGGTAAAACACCGTTGCATCTAAGACTAAGGTCGTCTGGTTGGCGTCCATCGG > KT2440/1935290‑1935526 | gCGATCCAGGAAGGGGCCAAGCCTGAAGCGGCCGCCAAGGACTGGGTCGCCAAGCACCCTGATCGTGTGAAGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTAAACCCGCTCCt < 4:18553/137‑1 (MQ=255)gCGATCCAGGAAGGGGCCAAGCCTGAAGCGGCCGCCAAGGACTGGGTCGCCAAGCACCCTGATCGTGTGAAGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTAAACCCGCTCCt < 4:193931/137‑1 (MQ=255) cAAGCCTGAAGCGGCCGCCAAGGACTGGGTCGCCAAGCACCCTGATCGTGTGAAGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTTAAACCCCTCCTACGGGTGGGGGGTTaac > 7:32985/1‑135 (MQ=255) cAAGCCTGAAGCGGCCGCCAAGGACTGGGTCGCCAAGCACCCTGATCGTGTGAAGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTAAACCCGCt < 8:371249/117‑1 (MQ=255) cAAGCCTGAAGCGGCCGCCAAGGACTGGGTCGCCAAGCACCCTGATCGTGTGAAGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTAAACCCGCt > 7:371249/1‑117 (MQ=255) cAAGCCTGAAGCGGCCGCCAAGGACTGGGTCGCCAAGCACCCTGATCGTGTGAAGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTAAACCCGCTCCTACCGGTGCGGGTTTAca > 2:386103/1‑136 (MQ=255) cAAGCCTGAAGCGGCCGCCAAGGACTGGGTCGCCAAGCACCCTGATCGTGTGAAGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTAAAACCCCACCCAAGGGTGCGGGTTTaaa > 7:96808/1‑135 (MQ=255) aaGCCTGAAGCGGCCGCCAAGGACTGGGTCGCCAAGCACCCTGATCGTGTGAAGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTTAAACCCCGGCGACCGGTGCGGGttttaac > 3:113191/1‑133 (MQ=255) cTGAAGCGGCCGCCAAGGACTGGGTCGCCAAGCACCCTGATCGTGTGAAGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTATAACCCCTCCGACGGGGGCGGGGTTTACcgcgg > 8:169149/1‑136 (MQ=255) gccAAGGACTGGGTCGCCAAGCACCCTGATCGTGTGAAGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTTAACCCGCTCCTACGGGTGGGGGTTTTCCCGCGAACGGGtcatca > 5:99577/1‑137 (MQ=255) gccAAGGACTGGGTCGCCAAGCACCCTGATCGTGTGAAGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTAAACCCGCGTCTACGGGTGCGGGTTTTCCCGCGAACGGGgcatca > 4:278856/1‑137 (MQ=255) gccAAGGACTGGGTCGCCAAGCACCCTGATCGTGTGAAGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTAAAACCGCTCCGAAGGGTGGGGGTTTTCCCGCGAACGGGgcatga > 7:251334/1‑135 (MQ=255) gccAAGGACTGGGTCGCCAAGCACCCTGATCGTGTGAAGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTAAAACCGCGCCTACGGGTGCGGGTTTACCCGCGAACGGGtgatca > 5:422482/1‑137 (MQ=255) cGCCAAGCACCCTGATCGTGTGAAGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTAAAACCGCTCCTACGGGTGCGGGTTTACCCGCGAACGGGGCATCACGTTTTTACGCaaa > 5:345352/1‑136 (MQ=255) cGCCAAGCACCCTGATCGTGTGAAGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTAAAACCCCTCCGAACGGGGGGGGTTTTGCCGGGCACCGGGCAAGACGTTGTTAAGCCAt > 7:177000/1‑137 (MQ=255) gCCAAGCACCCTGATCGTGTGAAGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTTAAACCGCGCCGACGGGTGGGGGGTTTACCGCGAACCGGGCCTCACTTTGTTTAGCAAta > 5:187265/1‑136 (MQ=255) aGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTTAACCCGCACCTACGGGTGCGGGGTTTACCGCGCACGGGTCATCACTTTGTTACGCAATCGGTAAAACACCGTTGGATCTaa > 2:327352/1‑137 (MQ=255) aCTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTTAAACCCCTGCTACGGGTGGGGGTTTTACCGCGAACGGGTGATCACTTTGTTTCGCAATAGGTAAAACACCGTTGGATGTAAGAATAAgg > 1:284701/1‑137 (MQ=255) aCTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTTAAACCCCGCCTACGGGTGGGGGGTTTACCGCGACCGGGTGATCACTTTGTTACGCAATCGGTTAAACACCGGTGGATCTAAGCCTAAgg > 7:277627/1‑137 (MQ=255) aGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTTAAACCGCTCCTTCGGGTGGGGGGTTTCCCGCGGACGGGTCATGACTTTGTTACGCAATCGGGTAAACACCGTTGGATCTTAGCCTAAGGTCGTCTg > 2:93302/1‑137 (MQ=255) aGGGCCAATGTTGTTTCTTCGCGGGTAAACCCCCTCCGACGGGTGCGGGGTTTACCGCGCACGGGTCATCACGTTGTTAAGCAATAGGTAAAACACCGTTTCATCTAAGACTAAAGTCGTCTGGTTTGCGTACATCg > 6:339812/1‑137 (MQ=255) gggCCAATGTTGTTTCTTCGCGGGTAAAACCGCTCCGACGGGTGGGGGTTTAACCGCGCACGGGTGATAACGTTTTTACCCAATCGGTAAAACACCGGTGGATATAATCCTAATGTAGTCGGGTTGGGGTCCCTCgg > 2:350210/1‑137 (MQ=255) | GCGATCCAGGAAGGGGCCAAGCCTGAAGCGGCCGCCAAGGACTGGGTCGCCAAGCACCCTGATCGTGTGAAGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTAAACCCGCTCCTACGGGTGCGGGTTTACCCGCGAACGGGTCATCACTTTGTTACGCAATCGGTAAAACACCGTTGCATCTAAGACTAAGGTCGTCTGGTTGGCGTCCATCGG > KT2440/1935290‑1935526 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |