| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 1,935,441 | 0 | A | T | 44.0% | 12.8 / 13.9 | 25 | intergenic (+62/‑143) | CJ019_01850/yjcH_1 | hypothetical protein/Inner membrane protein YjcH |
| Reads supporting (aligned to +/- strand): ref base A (12/2); new base T (11/0); total (23/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.87e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAAGCCTGAAGCGGCCGCCAAGGACTGGGTCGCCAAGCACCCTGATCGTGTGAAGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTAAACCCGCTCCTACGGGTGCGGGTTTACCCGCGAACGGGTCATCACTTTGTTACGCAATCGGTAAAACACCGTTGCATCTAAGACTAAGGTCGTCTGGTTGGCGTCCATCGGCAGCATAAAGTAAGGGTGTGGGTTCCATCCCGTATCTGTGCTGCTAGG > KT2440/1935307‑1935574 | cAAGCCTGAAGCGGCCGCCAAGGACTGGGTCGCCAAGCACCCTGATCGTGTGAAGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTTAAACCCCTCCTACGGGTGGGGGGTTaac > 7:32985/1‑135 (MQ=255)cAAGCCTGAAGCGGCCGCCAAGGACTGGGTCGCCAAGCACCCTGATCGTGTGAAGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTAAACCCGCTCCTACCGGTGCGGGTTTAca > 2:386103/1‑136 (MQ=255)cAAGCCTGAAGCGGCCGCCAAGGACTGGGTCGCCAAGCACCCTGATCGTGTGAAGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTAAAACCCCACCCAAGGGTGCGGGTTTaaa > 7:96808/1‑135 (MQ=255) cTGAAGCGGCCGCCAAGGACTGGGTCGCCAAGCACCCTGATCGTGTGAAGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTATAACCCCTCCGACGGGGGCGGGGTTTACcgcgg > 8:169149/1‑136 (MQ=255) gccAAGGACTGGGTCGCCAAGCACCCTGATCGTGTGAAGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTTAACCCGCTCCTACGGGTGGGGGTTTTCCCGCGAACGGGtcatca > 5:99577/1‑137 (MQ=255) gccAAGGACTGGGTCGCCAAGCACCCTGATCGTGTGAAGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTAAACCCGCGTCTACGGGTGCGGGTTTTCCCGCGAACGGGgcatca > 4:278856/1‑137 (MQ=255) gccAAGGACTGGGTCGCCAAGCACCCTGATCGTGTGAAGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTAAAACCGCTCCGAAGGGTGGGGGTTTTCCCGCGAACGGGgcatga > 7:251334/1‑135 (MQ=255) gccAAGGACTGGGTCGCCAAGCACCCTGATCGTGTGAAGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTAAAACCGCGCCTACGGGTGCGGGTTTACCCGCGAACGGGtgatca > 5:422482/1‑137 (MQ=255) cGCCAAGCACCCTGATCGTGTGAAGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTAAAACCGCTCCTACGGGTGCGGGTTTACCCGCGAACGGGGCATCACGTTTTTACGCaaa > 5:345352/1‑136 (MQ=255) cGCCAAGCACCCTGATCGTGTGAAGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTAAAACCCCTCCGAACGGGGGGGGTTTTGCCGGGCACCGGGCAAGACGTTGTTAAGCCAt > 7:177000/1‑137 (MQ=255) gCCAAGCACCCTGATCGTGTGAAGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTTAAACCGCGCCGACGGGTGGGGGGTTTACCGCGAACCGGGCCTCACTTTGTTTAGCAAta > 5:187265/1‑136 (MQ=255) aGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTTAACCCGCACCTACGGGTGCGGGGTTTACCGCGCACGGGTCATCACTTTGTTACGCAATCGGTAAAACACCGTTGGATCTaa > 2:327352/1‑137 (MQ=255) aCTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTTAAACCCCTGCTACGGGTGGGGGTTTTACCGCGAACGGGTGATCACTTTGTTTCGCAATAGGTAAAACACCGTTGGATGTAAGAATAAgg > 1:284701/1‑137 (MQ=255) aCTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTTAAACCCCGCCTACGGGTGGGGGGTTTACCGCGACCGGGTGATCACTTTGTTACGCAATCGGTTAAACACCGGTGGATCTAAGCCTAAgg > 7:277627/1‑137 (MQ=255) aGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTTAAACCGCTCCTTCGGGTGGGGGGTTTCCCGCGGACGGGTCATGACTTTGTTACGCAATCGGGTAAACACCGTTGGATCTTAGCCTAAGGTCGTCTg > 2:93302/1‑137 (MQ=255) aGGGCCAATGTTGTTTCTTCGCGGGTAAACCCCCTCCGACGGGTGCGGGGTTTACCGCGCACGGGTCATCACGTTGTTAAGCAATAGGTAAAACACCGTTTCATCTAAGACTAAAGTCGTCTGGTTTGCGTACATCg > 6:339812/1‑137 (MQ=255) gggCCAATGTTGTTTCTTCGCGGGTAAAACCGCTCCGACGGGTGGGGGTTTAACCGCGCACGGGTGATAACGTTTTTACCCAATCGGTAAAACACCGGTGGATATAATCCTAATGTAGTCGGGTTGGGGTCCCTCgg > 2:350210/1‑137 (MQ=255) tACGGGTGCGGGTTTACCCGCGAACGGGTCATCACTTTGTTACGCAATCGGTAAAACACCGTTGCATCTAAGACTAAGGTCGTCTGGTTTGCGTCCATCGGCAGCATAAAGTAAGGGTGTGGGTTCCAGACCGTAta > 7:169454/1‑136 (MQ=255) tACGGGTGCGGGTTTACCCGCGAACGGGTCATCACTTTGTTACGCAATCGGTAAAACACCGTTGCATCTAAGACTAAGGTCGTCTGGTTGGCGTTCATCGGCAGCATAAAGTAAGGGTGTGGGTTCCATCCCGTATc > 8:250858/1‑137 (MQ=255) tACGGGTGCGGGTTTACCCGCGAACGGGTCATCACTTTGTTACGCAATCGGTAAAACACCGTTGCATCTAAGACTAAGGTCGTCTGGTTGGCGTCCATCGGCAGCATAAAGTAAGGGTGTGGGTTCCAGCCCGTATc > 1:372793/1‑137 (MQ=255) cGGGTTTACCCGCGAACGGGTCATCACTTTGTTACGCAATCGGTAAAACACCGTTGCa < 7:321335/58‑1 (MQ=255) cGGGTTTACCCGCGAACGGGTCATCACTTTGTTACGCAATCGGTAAAACACCGTTGCa > 8:321335/1‑58 (MQ=255) ggTTTACCCGCGAACGGGTCATCACTTTGTTACGCAATCGGTAAAACACCGTTGCATCTAAGACTAAGGTCGTCTGGTTGGCGTCCATCGGCAGCATaaa > 6:35815/1‑100 (MQ=255) ggTTTACCCGCGAACGGGTCATCACTTTGTTACGCAATCGGTAAAACACCGTTGCATCTAAGACTAAGGTCGTCTGGTTGGCGTCCATCGGCAGCATaaa < 5:35815/100‑1 (MQ=255) ggTTTACCCGCGAACGGGTCATCACTTTGTTACGCAATCGGTAAAACACCGTTGCATCTAAGACTAAGGTCGTCTGGTTGGCGTCCATCGGCAGCATAAAGTAAGGGTGTGGGTTCCATCCCGTATCTGTGCTGCTa > 4:127606/1‑137 (MQ=255) tttACCCGCGAACGGGTCATCACTTTGTTACGCAATCGGTAAAACACCGTTGCATCTAAGACTAAGGTCGTCTGGTTGGCGTCCATCGGCAGCATAAAGTAAGGGTGTGGGTTCCATCCCGTATCTGTGCTGCTAgg > 6:410978/1‑137 (MQ=255) tttACCCGCGAACGGGTCATCACTTTGTTACGCAATCGGTAAAACACCGTTGCATCTAAGACTAAGGTCGTCTGGTTGGCGTCCATCGGCAGCATAAAGTAAGGGTGTGGGTTCCATCCCGTATCTGTGCTGCTAgg > 6:403583/1‑137 (MQ=255) | CAAGCCTGAAGCGGCCGCCAAGGACTGGGTCGCCAAGCACCCTGATCGTGTGAAGGAGTGGACTGGCAAGTAAAAGCCTTCCAGGGCCAATGTTGTTTCTTCGCGGGTAAACCCGCTCCTACGGGTGCGGGTTTACCCGCGAACGGGTCATCACTTTGTTACGCAATCGGTAAAACACCGTTGCATCTAAGACTAAGGTCGTCTGGTTGGCGTCCATCGGCAGCATAAAGTAAGGGTGTGGGTTCCATCCCGTATCTGTGCTGCTAGG > KT2440/1935307‑1935574 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |