Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	2,308,321	0	C	G	34.9%	75.1 / 21.9	64	A230P (GCC→CCC)	fucA	L‑fuculose phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base C (18/23);  new base G (22/0);  total (40/24)
Fisher's exact test for biased strand distribution p-value = 3.47e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACCTTTGCCGTTGTTGAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGATCAGCAGGCCA  >  KT2440/2308190‑2308457                                                                                                                                    |                                                                                                                                          aCCTTTGCCGTTGTTGAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCAC‑tt                                                                                                                                     >  7:364527/1‑137 (MQ=255)  ccTTTGCCGTTGTTGAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCTA‑ttt                                                                                                                                    >  8:26950/1‑137 (MQ=255)  ccTTTGCCGTTGTTGAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑ttt                                                                                                                                    >  2:15710/1‑137 (MQ=255)  ccTTTGCCGTTGTTGAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑ttt                                                                                                                                    <  6:130702/137‑1 (MQ=255)  ccTTTGCCGTTGTTGAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑ttt                                                                                                                                    <  5:316343/137‑1 (MQ=255)    tttGCCGTTGTTGAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGc                                                                                                                                  <  6:196503/137‑1 (MQ=255)             gttgAGGAAAGAGTACAGCGTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCTCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGTTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCtt                                                                                                                         <  4:53108/137‑1 (MQ=255)                gAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑ttt                                                                                                                                    <  8:53387/123‑1 (MQ=255)                gAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑ttt                                                                                                                                    >  7:53387/1‑123 (MQ=255)                     aaGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATg                                                                                                                 <  7:349912/137‑1 (MQ=255)                         gTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCGC‑TTTGGCCAGTGCTTGGGGGATggggg                                                                                                             >  3:240767/1‑136 (MQ=255)                         gTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCGC‑GTTGCGCCGTGggtggggggtggggg                                                                                                             >  3:115886/1‑136 (MQ=255)                           aCAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGCATTTGCCC‑GTGGTTCGGGGGTGGGGCgg                                                                                                           >  6:61888/1‑137 (MQ=255)                           aCAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTG‑TTTGCCCAGTGCTTCGGGGTTTGGGCgg                                                                                                           >  6:458504/1‑137 (MQ=255)                           aCAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCgg                                                                                                           >  8:67679/1‑137 (MQ=255)                                 tCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGG‑TTTGGCCAGTGCTTCGGGGGTGGGGCGGgtgtga                                                                                                     >  7:365557/1‑135 (MQ=255)                                  cGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCTC‑TTTGGCCAGTGCTTCGGGGCTGGGGCGGCTgggac                                                                                                    >  8:57638/1‑133 (MQ=255)                                  cGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGc                                                                                                                                  <  5:372249/107‑1 (MQ=255)                                  cGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGc                                                                                                                                  >  6:372249/1‑107 (MQ=255)                                    aTCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGC‑TTTGCCCAGTGATTCGGGGCTGGGGCGGATGTAACCg                                                                                                  >  6:234190/1‑137 (MQ=255)                                       aGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGGGC‑GTTGGCCCGTGCGTCGGGGGTGGGGGGGGTGTGACCcgcg                                                                                               >  1:245536/1‑137 (MQ=255)                                       aGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑GTTGCCCAGTGGGTAGGGGATGGGGCGGATGTCACCGgcg                                                                                               >  6:380163/1‑137 (MQ=255)                                       aGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCGC‑AATTCCCCGTGCGTCGGGGATGGGGCGGATGTCACCGgcg                                                                                               >  6:450155/1‑137 (MQ=255)                                            aGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCGC‑TTTGCCCAGTGGGTGGGGGATGGGGCGGGGGTCACCGGCGcccca                                                                                          >  6:356/1‑135 (MQ=255)                                               cAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGc                                                                                       <  3:451053/137‑1 (MQ=255)                                                     ccATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCTC‑TTTGCCCAGTGGTTCGGGGGTGGGGGGGCTGTCACCGGCGCCCATGGCcagcag                                                                                 >  6:416451/1‑137 (MQ=255)                                                     ccATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGG‑TTTGCCCAGGGGTTAGGGGATGGGGCGGATGTGCCCGGCGCCCAAGGCcagcag                                                                                 >  8:23080/1‑137 (MQ=255)                                                     ccATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGC‑GTTGGCCAGTGGTTGGGGGTTGGGGGGGGTGTGAACGGCGCCCAGGGCcacgcc                                                                                 >  5:270173/1‑133 (MQ=255)                                                     ccATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGGTTCGGGGGTGGGGGGGATGTGACCGGCGCCCAAGGGccgcag                                                                                 >  7:341825/1‑137 (MQ=255)                                                     ccATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCcagcag                                                                                 <  3:77800/137‑1 (MQ=255)                                                     ccATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑GTTGCCCAGTGCTTAGGGGCTGGGGCGGATGTCACCGGCGCCCCAGGCcaccag                                                                                 >  8:203813/1‑137 (MQ=255)                                                      cATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCTG‑GTTTACCAGTGCGTAGGGGAAGGGGGGGCTGGAACCGGCGCCCCTGGCCAGGAGt                                                                                >  6:133824/1‑137 (MQ=255)                                                      cATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCTC‑TTTGGCCAGTGCTTCGGGGGTGGGGCGGATGTCACCGGCGCCCATGGCCAGCCGt                                                                                >  5:158024/1‑137 (MQ=255)                                                      cATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGC‑TTTGGCCACTGGGTCGGGGCTGGGGCGGATGTGACCCGCGCCCAAGGCCAGCAGt                                                                                >  1:287944/1‑137 (MQ=255)                                                      cATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑GTTGGCCCGTGCGTCGGGGTTGGGGCGGGTGTGACCGGCGCCCATGGCCCGCAGt                                                                                >  8:52285/1‑137 (MQ=255)                                                           gCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGAACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCGC‑TTTTCCCACTGCTTCGGGGCTGGGGCGGATGTAACCGGCGCCCAATGCCACCAGTTGCAt                                                                           >  8:251567/1‑137 (MQ=255)                                                                cAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTtg                                                                      <  8:208050/137‑1 (MQ=255)                                                                 aaGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGC‑GTTTGGCACTGGGTGGGGGGTGGGGGGGGTGTTCGCGGCGCCCCAGGGCCGCACTTGGATATTtgt                                                                     >  1:361717/1‑136 (MQ=255)                                                                 aaGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGC‑GTTGCCCAGTGGTTCGGGGATGGGGGGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCttttc                                                                     >  6:219181/1‑135 (MQ=255)                                                                   ggCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGC‑TTTGCTCAGTGCTTCGGGGGTGGGGCGGATGTACCCCGCGCCCCAGGCCAGCAGTTGCATCttttgtt                                                                   >  7:87808/1‑133 (MQ=255)                                                                     cACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTgcg                                                                 <  6:176718/137‑1 (MQ=255)                                                                     cACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTgcg                                                                 <  7:251567/137‑1 (MQ=255)                                                                     cACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTAGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTgcg                                                                 <  6:22350/137‑1 (MQ=255)                                                                       cGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTTCCCAGTGCTTCGGGGGTGGGGCGGCTGTCACCGGCGCCCATTGCCACCAGTTGCATCTTTTCTgcgcg                                                               >  5:166573/1‑137 (MQ=255)                                                                        ggCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTgcgcgc                                                              <  6:41977/137‑1 (MQ=255)                                                                          cgcgcgTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGG‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTAACCGGCGCCCATGGCCAGCAGTTGCATCTTTTCTGGGCGCTc                                                            >  2:294705/1‑137 (MQ=255)                                                                          cgcgcgTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTc                                                            <  2:212229/137‑1 (MQ=255)                                                                            cgcgTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGC‑GTTTGCCAGGGGTTGGGGGGTGGGGGGGCTGTCCCCCGCGCCCCTGGGCCGCACGTGCAGATTTGGCGcgcgcgcgg                                                          >  1:364198/1‑136 (MQ=255)                                                                             gcgTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGaa                                                         <  5:70603/137‑1 (MQ=255)                                                                                  gtagCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAAcagca                                                    <  1:294705/137‑1 (MQ=255)                                                                                    agCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCaga                                                  <  8:65488/137‑1 (MQ=255)                                                                                             cagcaCCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACg                                         <  4:65807/137‑1 (MQ=255)                                                                                              agcaCCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCGC‑TTTGGCCAGGGGGTCGGGGGGGGGGGGGGTGTCACCGGCGCCCAAGGGCAGCAGTTGGATCATTGCTGCGCGCTCGCACAGCAGAGCGAGCCCGc                                        >  2:123402/1‑137 (MQ=255)                                                                                                   cGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGGCTG‑TTTTCCCAGTGCTTCGGGGATGGGGGGGGTGTCACCGGCGCCCCTGGCCAGCAGTTGCATCTTTGGTGCGCGCTCGAACAGCAGAGCGAGCACGCCGGcg                                   >  4:336870/1‑136 (MQ=255)                                                                                                        cGTTTGGGGGTGGAAATCCAGTCGTGGGGCTC‑ATTGCCCACTGCTTCGGGGCTGGGGCGGATGTCACCCGCGCCCCTTGCCAGCACTTGCATATTTGCTGCGCGCTCCCACAGCAGCGCGAGCCCCCAGGCGTCCtc                              >  4:328838/1‑137 (MQ=255)                                                                                                        cGTTTGGGGGTGGAAATCCAGTCGTGGGGCGC‑TTTGGCCACTGGATCGGGGATGGGGGGGATGTCACCCGCGCCCATGGCCCGCACTTGCCTATTTTCTTCGCGCTCGAACAGCAGAGCGAGCACGCACGCTTCCta                              >  6:475450/1‑136 (MQ=255)                                                                                                        cGTTTGGGGGTGGAAATCCAGTCGTGGGCCGC‑GTTGCCCAGTGCTTAGGGGCTGGGGCGGATGTGCCCCGCGCCCATGGCCCGCAGTTGGATCTTTTCTTCGCGCTCGAACAGCAGAGCGAGCCCGCAGGcgtacta                              >  7:426855/1‑131 (MQ=255)                                                                                                             gggggTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCGTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTTCATCTTTTCTGCGCGCTCGAACACCACCGCGAGCACGCAGGCATGCtcgagac                         >  4:275436/1‑134 (MQ=255)                                                                                                                   ggAAATCCAGTCGTGGGCCGA‑TTTGGCCAGTGCTTAGGGGATTGGGGGGGTGGGACCGGCGCCCCAGGCCCGGAGTTGGAGATTTGGAGGGCGAGCGAACACCAGAGCGCTCCATCAGGCTTGCTCGATCGAACGcc                   >  2:147745/1‑137 (MQ=255)                                                                                                                     aaaTCCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCa                 <  7:67679/137‑1 (MQ=255)                                                                                                                          cAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGAt            <  1:15710/137‑1 (MQ=255)                                                                                                                          cAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGAt            <  2:74875/137‑1 (MQ=255)                                                                                                                          cAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGAt            <  2:458504/137‑1 (MQ=255)                                                                                                                           aGTCGTGGGACTC‑TTTGCGCTGTTCTTCGGGGATGTGTCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCTAAAAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGACCAGCGATc           <  7:26950/137‑1 (MQ=255)                                                                                                                                    ccTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGATCAGCAGGCCa  <  4:153065/137‑1 (MQ=255)                                                                                                                                    |                                                                                                                                          ACCTTTGCCGTTGTTGAGGAAAGAGTACAGCTTCGATCAGCCCAGGCAGTCGCCATGGGCACGCAAGGCACGGCGCGCGTAGTAGCTGAAGGCAGCACCGTGACGTTTGGGGGTGGAAATCCAGTCGTGGGCCTC‑TTTGCCCAGTGCTTCGGGGATGGGGCGGATGTCACCGGCGCCCATGGCCAGCAGTTGCATCTTTGCTGCGCGCTCGAACAGCAGAGCGAGCACGCAGGCTTCCTCGATCGAACGCCCAGCGATCAGCAGGCCA  >  KT2440/2308190‑2308457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑