Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,583,3160CA32.5% 58.7 / 11.5 40I113I (ATC→ATA) CJ019_02435hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (20/7);  new base A (1/12);  total (21/19)
Fisher's exact test for biased strand distribution p-value = 1.07e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGCCGTGAAGAGTTCAAGAAGCTCAAGATCGAAGCCCGCAAGAAGCTGAAAGACGTAACCGTGCAGCCGCTCTTCACCGAGGAGTACGACAAGGAGACCGAGGAGCCGACTGGCCGCCTGTTCTTCAACATCAAAATGGCCGCCTCGGGCAAGTCGAAGAAAGACGGCTCCGTCTGGACCCGCAAGCCAGCCCTGTTCGATGCCAAGGGCAAGCCATTGCTGAAAGTCCCGGACATCTGGGGCGGCACCGAAGGCAAGGTGA  >  KT2440/2583185‑2583446
                                                                                                                                   |                                                                                                                                  
ggCCGTGAAGAGTTCAAGAAGCTCAAGATCGAAGCCCGCAAGAAGCTGAAAGACGTAACCGTGCAGCCGCTCTTCACCGAGGAGTACGACAAGGAGACCGAGGAGCCGACTGGCCGCCTGTTCTTCAACATCAAAAt                                                                                                                               <  8:261156/137‑1 (MQ=255)
   cGTGAAGAGTTCAAGAAGCTCAAGATCGAAGCCCGCAAGAAGCTGAAAGACGTAACCGTGCAGCCGCTCTTCACCGAGGAGTACGACAAGGAGACCGAGGAGCCGACTGGCCGCCTGTTCTTCAACATCAAAATGgc                                                                                                                            >  4:86003/1‑137 (MQ=255)
     agacaagTTCAAGAAGCTCAAGATCGAAGCCCGCAAGAAGCTGAAAGACGTAACCGTGCAGCCGCTCTTCACCGAGGAGTACGACAAGGAGACCGAGGAGCCGACTGGCCGCCTGTTCTTCAACATCAAAATGgccg                                                                                                                          <  1:446270/132‑1 (MQ=255)
       aaGAGTTCAAGAAGCTCAAGATCGAAGCCCGCAAGAAGCTGAAAGACGTAACCGTGCAGCCGCTCTTCACCGAGGAGTACGACAAGGAGACCGAGGAGCCGACTGGCCGCCTGTTCTTCAACATAAAAATGgccgcc                                                                                                                        >  6:306109/1‑137 (MQ=255)
         gagTTCAAGAAGCTCAAGATCGAAGCCCGCAAGAAGCTGAAAGACGTAACCGTGCAGCCGCTCTTCACCGAGGAGTACGACAAGGAGACCGAGGAGCCGACTGGCCGCCTGTTCTTCAACATCAAAATGGCCGCCTc                                                                                                                      >  3:94408/1‑137 (MQ=255)
                       cAAGATCGAAGCCCGCAAGAAGCTGAAAGACGTAACCGTGCAGCCGCTCTTCACCGAGGAGTACGACAAGGAGACCGAGGAGCCGACTGGCCGCCTGTTCTTCAACATCAAAATGGCCGCCTCGGGCAAGTCgaaga                                                                                                        >  5:298987/1‑137 (MQ=255)
                         aGATCGAAGCCCGCAAGAAGCTGAAAGACGTAACCGTGCAGCCGCTCTTCACCGAGGAGTACGACAAGGAGACCGAGGAGCCGACTGGCCGCCTGTTCTTCAACATCAAAATGGCCGCCTCGGGCAAGTCGaagaaa                                                                                                      >  7:203848/1‑137 (MQ=255)
                            tctaaGCCCGAAAGAATCTTAAAGACGTAACCGTGCAGCCGCTCTGAACCGAGGAGTACTACAAGGAGCCCGAGGAGCCGACTGGCCGCCTGTTCTTCAAAATAAAAATGGCCGCCTCGGGAAAGTCTAATAAAGAc                                                                                                   <  6:298987/134‑1 (MQ=255)
                                  cccGCAAGAAGCTGAAAGACGTAACCGTGCAGCCGCTCTTCACCGAGGAGTACGACAAGGAGACCGAGGAGCCGACTGGCCGCCTGTTCTTCAACATCAAAATGGCCGCCTCGGGCAAGTCGAAGAAAGACGGCTcc                                                                                             >  4:42630/1‑137 (MQ=255)
                                         gaagCTGAAAGACGTAACCGTGCAGCCGCTCTTCACCGAGGAGTACGACAAGGAGACCGAGGAGCCGACTGGCCGCCTGTTCTTCAACATCAAAATGGCCGCCTCGGGCAAGTCGAAGAAAGACGGCTCCGTCTGGa                                                                                      >  8:369622/1‑137 (MQ=255)
                                         gaagCTGAAAGACGTAACCGTGCAGCCGCTCTTCACCGAGGAGTACGACAAGGAGACCGAGGAGCCGACTGGCCGCCTGTTCTTCAACATCAAAATGGCCGCCTCGGGCAAGTCGAAGAAAGACGGCTCCGTCTGGa                                                                                      >  6:385300/1‑137 (MQ=255)
                                          acgCTCAAAGACATACCCGTGCAGCCGCGCTACCCGTCGTATTACCACACGGCGACCGCGCAGCCTACTGGCCGCCGGTTCTTCAACAAAAAAATGCCCGCCTCGGGCAAGTCGAACAACGACGGCTCCGTCTGGAc                                                                                     <  6:227400/135‑1 (MQ=255)
                                          aagGTGAACTACTTAACCGGGCCGCCGCGCTACACCTAGGAGTACGACAAGGAGCCCGCGGAGCCGACGGGCCGCCTGTTCTTCAACATCAAAATGGCCGCCGCGGGCAAGTCTAATAAAGACGGCTCCGTCTGGAc                                                                                     <  8:327602/137‑1 (MQ=255)
                                          aagATGAAATCCGTACCCGTGCAGCCGCTCTGCACCGAGGAGGACGACCAGGAGCCCGCGGAGCCGACTGGCCGCCTTTTCTTCAACATAAAAATGGCCGCCTCGGGAAAGTCGAAGAAAGACGGCTCCGTCTGGAc                                                                                     <  8:236147/137‑1 (MQ=255)
                                          aaccTGAAAGACGAAACCGCGCCGCCGCTCTCCACCGAGGAGACCCACACGGCGACCGAGGAGCCGACGGGCCGCCTGTTCTTCAACATAAAAATGCCCGCCTCGGGCAAGTCGAACAACGACGGCTCCGTCTGGAc                                                                                     <  2:127389/134‑1 (MQ=255)
                                               gAAAGACGTAACCGTGCAGCCGCTCTTCACCGAGGAGTACGACAAGGAGACCGAGGAGCCGACTGGCCGCCTGTTCTTCAACATCAAAATGGCCGCCTCGGGCAAGTCGAAGAAAGACGGCTCCGTCTGGACCCGCa                                                                                >  4:367242/1‑137 (MQ=255)
                                               caaaGACGTAACCGTGCAGCCGCTCTTCACCGTGGAGAACGACAAGGAGCCCGAGGAGCCGAGTGGCCGCCTGTTCTTAAACATAAAAATGGCCGCCTCGGGCAAGTCGAAGAAAGACGGCTCCGTCTGGACCCGCa                                                                                <  2:385771/136‑1 (MQ=255)
                                                    aCGAAACCGTGCAGCCGCTCTTCACCGAGGAGTACGACAAGGAGACCGAGGAGCCGAGTGGCCGCCTGTTCTTCAACATCAAAATGGCCGCCTCGGGAAAGTCGAAGAAAGACGGCTCCGTCTGGACCCGCAagcca                                                                           <  2:241235/137‑1 (MQ=255)
                                                           cGTGCAGCCGCTCTTCACCGAGGAGTACGACAAGGAGACCGAGGAGCCGACTGGCCGCCTGTTCTTCAACATCAAAATGGCCGCCTCGGGCAAGTCGAAGAAAGACGGCTCCGTCTGGACCCGCAAGCCAGCCCTGt                                                                    >  3:26941/1‑137 (MQ=255)
                                                                cgCCGCTCTTCACCGTGGATTCCTACCAGGAGACCGAGTCGCCGACTGGCCGACTTTTCTTCAAAATAAAAATGGCCGCCGCGGGCAAGTCCAAGACCCACGGCTCCGTCTGGACCCGCAAGCCAGCCCTGTTCGAt                                                               <  8:203848/136‑1 (MQ=255)
                                                                cgCCGCTATTCACGGCGGATTACCAAAAGCAGCCCTAGGCGCCGACTGGCCGCCTGTTCTTAAACATAAAACGGGCCGCCGCGGGCACGTCCAACAAAGACGGCTCCGTCTGGACCCGCAAGCCAGCCCTGTTCGAt                                                               <  2:416323/136‑1 (MQ=255)
                                                                aGCCGCTCTTCACCGAGGAGTACGACAAGGAGACCGAGGAGCCGACTGGCCGCCTGTTCTTCAACATCAAAATGGCCGCCTCGGGCAAGTCGAAGAAAGACGGCTCCGTCTGGACCCGCAAGCCAGCCCTGTTCGAt                                                               >  3:15535/1‑137 (MQ=255)
                                                                  ccGCTCTTCACCGAGGAGTACGACAAGGAGACCGAGGAGCCGACTGGCCGCCTGTTCTTCAACATCAAAATGGCCGCCTCg                                                                                                                     <  8:279157/81‑1 (MQ=255)
                                                                  ccGCTCTTCACCGAGGAGTACGACAAGGAGACCGAGGAGCCGACTGGCCGCCTGTTCTTCAACATCAAAATGGCCGCCTCg                                                                                                                     >  7:279157/1‑81 (MQ=255)
                                                                  ccGCTCTTCACCGAGGAGTACGACAAGGAGACCGAGGAGCCGACTGGCCGCCTGTTCTTCAACATCAAAATGGCCGCCTCGGGCAAGTCGAAGAAAGACGGCTCCGTCTGGACCCGCAAGCCAGCCCTGTTCGATGc                                                             >  3:225519/1‑137 (MQ=255)
                                                                  ccGCTCTTCACCGAGGAGTACGACAAGGAGACCGAGGAGCCGACTGGCCGCCTGTTCTTCAACATCAAAATGGCCGCCTCGGGCAAGTCGAAGAAAGACGGCTCCGTCTGGACCCGCAAGCCAGCCCTGTTCGATGc                                                             >  8:144598/1‑137 (MQ=255)
                                                                  ccGCTCTTCACCGACGAGTACGACAAGGAGACCGAGGAGCCGACTGGCCGCCTGTTCTTAAACATCAAAATGGCAGCCTCGGGCAAGACGAAGAAAGACGGCTCCGTCTCGACCCGCAAGCCAGCCCTGTTCGATGc                                                             >  5:407397/1‑137 (MQ=255)
                                                                                  agTACGACAAGGAGACCGAGGAGCCGACTGGCCGCCTGTTCTTCAACATCAAAATGGCCGCCTCGGGCAAGTCGAAGAAAGACGGCTCCGTCTGGACCCGCAAGCCAGCCCTGTTCGATGCCAAGGGCAAGCCATTg                                             >  7:252665/1‑137 (MQ=255)
                                                                                   gaaccaCAAGGACCCCGAGGCGCCGACTGGCCGCCTGTTCTTCAACATCAAAATGGCCGCCTCGGGCAAGTCGAAGAAAGACGGCTCCGTCTGGACCCGCAAGCCAGCCCTGTTCGATGCCAAGGGCAAGCCATTGc                                            <  6:21884/132‑1 (MQ=255)
                                                                                           cggcgcccGCGGCGCCGACTGGCCGCTTTTTCTTAAACAGAAAAATGCCCGCCTCGGGAAAGTCCACGAACCACGGCTCCGTCTGGACCCGCAAGCCAGCCCTGTCCGATGCCAAGGGCAAGCCATTGCTGAAAGTc                                    <  4:77854/131‑1 (MQ=255)
                                                                                               gaCCGAGGAGCCGACTGGCCGCCTGTTCTTCAACATCAAAATGGCCGCCTCGGGCAAGTCGAAGAAAGACGGCTCCGTCTGGACCCGCAAGCCAGCCCTGTTCGATGCCAAGGGCAAGCCATTGCTGAAAGTCCCgg                                >  1:43781/1‑137 (MQ=255)
                                                                                               gaCCGAGGAGCCGACTGGCCGCCTGTTCTTCAACATCAAAATGGCCGCCTCGGGCAAGTCGAAGAAAGACGGCTCCGTCTGGACCCGCAAGCCAGCCCTGTTAGATGCCAAGGGCAAGCCATTGCTGAAAGTCCCgg                                >  1:456256/1‑137 (MQ=255)
                                                                                                            agtGGCCGCGTGTTATTCAAAATAAAAATGGCCGACTCAGCCAAGTCCAAGAAAGACGGCTCCGTCTGGACCCGCAAGCCAGCCCTGTTCGATGCCAAGGGCAAGCCATTGCTGAAAGTCCCGGACATCTGGggcgg                   <  5:44711/135‑1 (MQ=255)
                                                                                                            aCTGGCCGCCTGTTCTTCAACATCAAAATGGCCGCCTCGGGCAAGTCGAAGAAAGACGGCTCCGTCTGGACCCGCAAGCCAGCCCTGTTCGATGCCAAGGGCAAGCCATTGCTGAAAGTCCCGGACATCTGGggcgg                   >  3:138961/1‑137 (MQ=255)
                                                                                                               ggCCGCCTGTTCTTCAACATCAAAATGGCCGCCTCGGGCAAGTCGAAGAAAGACGGCTCCGTCTGGACCCGCAAGCCAGCCCTGTTCGATGCCAAGGGCAAGCCATTGCTGAAAGTCCCGGACATCTGGGGCGGCAc                >  2:388422/1‑137 (MQ=255)
                                                                                                                   gccTTTTCTTAAAAATAAAAATGGCCCCCTCGGGAAAGTCTAATAACGACGGCTCCGTCTGGACCCGCAAGCCAGCCCTGTTCGATGCCAAGGGCAAGCCATTGCTGAAAGTCCCGGACATCTGGGGCGGCACCGaa            <  4:405421/137‑1 (MQ=255)
                                                                                                                        ttcttcAACATCAAAATGGCCGCCTCGGGCAAGTCGAAGAAAGACGGCTCCGTCTGGACCCGCAAGCCAGCCCTGTTCGATGCCAAGGGCAAGCCATTGCTGAAAGTCCCGGACATCTGGGGCGGCACCGAAGGCaa       <  5:385300/137‑1 (MQ=255)
                                                                                                                        ttcttcAACATAAAAATGGCCGCCTCGGTCAAGTCGAATAAAGACGGCTCCGTCTGGACCCGCAAGCCAGCCCTGTTCGATGCCAAGGGCAAGCCATTGCTGAAAGTCCCGGACATCTGGGGCGGCACCGAAGGCaa       <  4:158440/137‑1 (MQ=255)
                                                                                                                             cAACATCAAAATGGCCGCCTCGGGCAAGTCGAAGAAAGACGGCTCCGTCTGGACCCGCAAGCCCGCCCTGTTCGATGCCAAGGGCAAGCCATTGCGGAAAGTCCCGGACATCTGGGGCGGCACCGAAGGCAACGTgg  >  4:432203/1‑136 (MQ=255)
                                                                                                                             aaaCATAAAAATGGCCGCCGCGGGCAAGTCTAAGAACGACGGCTCCGTCTGGACCCGCAAGCCAGCCCTGTTCGATGCCAAGGGCAAGCCATTGCTGAAAGTCCCGGACATCTGGGGCGGCACCGAAGGCAAGGTGa  <  4:26941/136‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                  
GGCCGTGAAGAGTTCAAGAAGCTCAAGATCGAAGCCCGCAAGAAGCTGAAAGACGTAACCGTGCAGCCGCTCTTCACCGAGGAGTACGACAAGGAGACCGAGGAGCCGACTGGCCGCCTGTTCTTCAACATCAAAATGGCCGCCTCGGGCAAGTCGAAGAAAGACGGCTCCGTCTGGACCCGCAAGCCAGCCCTGTTCGATGCCAAGGGCAAGCCATTGCTGAAAGTCCCGGACATCTGGGGCGGCACCGAAGGCAAGGTGA  >  KT2440/2583185‑2583446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑