Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,134,5670TG37.0% 45.6 / 11.4 54L143V (TTG→GTG) CJ019_02952hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (22/12);  new base G (20/0);  total (42/12)
Fisher's exact test for biased strand distribution p-value = 1.92e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGAT  >  KT2440/3134432‑3134703
                                                                                                                                       |                                                                                                                                        
cTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGtt                                                                                                                                         >  2:428892/1‑137 (MQ=255)
  cgttACCCGGAGCGTGATCGACCCCTTGCTCAAGCTCATCAAGGCATCATGTATCCGGCTTTTCACCATTGATGTGCCATCGAAGTACAGCCTAAACACCACCACCTGGTACAACGTCGCGGCCGGCGGCGGGTTgg                                                                                                                                       <  6:311668/134‑1 (MQ=255)
     tACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCAACGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCCGCCGCCCGCCCCGTGGccc                                                                                                                                    >  1:246512/1‑136 (MQ=255)
             gCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGAACAGCCTCAACAACACCACCTCGGACACCGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTg                                                                                                                            <  7:466932/137‑1 (MQ=255)
               gTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGCACAGCTGGt                                                                                                                          >  4:75674/1‑137 (MQ=255)
               gTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTggg                                                                                                                          >  7:384208/1‑136 (MQ=255)
               gTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGt                                                                                                                          >  8:94220/1‑137 (MQ=255)
               gTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCCCGGTGGCCGCACACCAGGt                                                                                                                          >  1:42802/1‑137 (MQ=255)
               gTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCCCGCGTTGGCCCAACCCCTggg                                                                                                                          >  3:25235/1‑136 (MQ=255)
               gTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCCCGCGGTGGCCGCACAGCTggg                                                                                                                          >  3:99844/1‑136 (MQ=255)
               gTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCCCGGCCGGCCGCGCGGGGGCCGCACACCTCGt                                                                                                                          >  3:413606/1‑137 (MQ=255)
               gTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGACATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCCCGGAGGCCGAACaccaggg                                                                                                                          >  6:57075/1‑131 (MQ=255)
                tGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTg                                                                                                                         >  1:319403/1‑137 (MQ=255)
                     gACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGCACAGCTGGTGCGCGa                                                                                                                    >  6:299539/1‑137 (MQ=255)
                     gACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCCCGCGGTGGGCGCACCGCGGGGGcgcgc                                                                                                                    >  3:331982/1‑136 (MQ=255)
                     gACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCCGCCCCGCGGTGGCCGCACAGCTGGGGcgcgc                                                                                                                    >  7:148262/1‑136 (MQ=255)
                           tGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGGTGGGCGCACCGCGGGGGCGCGGCCCcgc                                                                                                              >  1:249126/1‑136 (MQ=255)
                                    cgcATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACCCCTGGTGCGCGCCACCGGCGGCAAggg                                                                                                     >  6:169752/1‑137 (MQ=255)
                                    cgcATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGACGAACAGCTGGTGCGCGCCACCGGCGGCAAggg                                                                                                     >  5:83888/1‑137 (MQ=255)
                                    cgcATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGGTGGCCGCCCCGCTGGTGCGCGGCACCGGCGGCAAcgg                                                                                                     >  4:200459/1‑137 (MQ=255)
                                    cgcATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCCCGCCGTGGCCGCACCGCAGGTGCGCGCCCCCGGCGGGCAggg                                                                                                     >  4:424812/1‑137 (MQ=255)
                                     gcATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGGTGGCCGAACAGCTGGTGCGCGTCACCGGCGGCAAgggg                                                                                                    >  8:295105/1‑136 (MQ=255)
                                              cagcagGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGGTGGCCGCACAGCTGGTGCGCGCCCCCGGCGGCAAGGGCCGCCTccgg                                                                                           >  7:269749/1‑135 (MQ=255)
                                                gcagGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCCCGCGGTGGCCGAACAGCTGGTGCGCGACCCCCGCGGCAAGGGCCGCATCCTGgt                                                                                         >  5:454126/1‑137 (MQ=255)
                                                  gggacccccGCTTTTCACCTTTTATGGCCCAGCGCAGAACCCCCACAACACCCCCCCCCCGCACACCGGCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGgtgt                                                                                       <  4:331982/131‑1 (MQ=255)
                                                    gTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCCGTGGCCGCACCGCTGGTGCGCGACACCGGCGGCAAGGGCCGCCGCCAGGTGTTc                                                                                     >  8:134063/1‑137 (MQ=255)
                                                          cGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGGTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGc                                                                               >  8:254704/1‑137 (MQ=255)
                                                          cGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGGTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCAGCCTGGTGTTCTACggg                                                                               >  1:360934/1‑136 (MQ=255)
                                                            tttttCACCATTGATGTGCCATCGCAGTACAGCCCCACCACCACCCCCTCGGACACCGGCGCGGCGGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCtt                                                                             <  8:148262/136‑1 (MQ=255)
                                                                    cATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCCGCCGCGCGGTGGCCGAACAGCTGGTGCGCGACACCCGCCGCAAGGGCCCCATCCAGGTGTTCAACGGCGTATACgggg                                                                     >  1:64119/1‑137 (MQ=255)
                                                                               cATCGCAGTACCCCCACAACACCCCCACCGCGGACACCGCCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCtgt                                                          <  7:348946/137‑1 (MQ=255)
                                                                               cATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGGCGAACACCTGGTGCGCGACACCCGCGGCAAGGGCCGCATCCAGGTGTTAAACGGCTTGTACGGGGTGCCGGTCtgt                                                          >  3:24687/1‑137 (MQ=255)
                                                                               aaTCGCAGAACCCCCTCAACACCCCCACCTCGCACAACGGCGCGGCCGGCCGCGGGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCtgt                                                          <  8:343130/136‑1 (MQ=255)
                                                                                    cAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGGGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGAt                                                     >  3:288569/1‑137 (MQ=255)
                                                                                          aGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGCACAGCTGGTGCGCGACACCCGCGGCAAGGGCCGCATACTGGTGTTCAACGGCTTAGACGGGGTGCCCGTCTGTGCGTTgcgcaa                                               >  8:1619/1‑135 (MQ=255)
                                                                                               caacaaCACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGCTCCGCTacgacg                                          >  7:387770/1‑137 (MQ=255)
                                                                                               caacaaCACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTacgacg                                          >  6:215728/1‑137 (MQ=255)
                                                                                                aacaacACCACCTCGCACACCGGCGCGGCCGGCCGCGTGTTGGCCTAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTAcgacga                                         <  8:294172/137‑1 (MQ=255)
                                                                                                  caacaCCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGCCCCCGGCGGCAATGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTGTGTGCGCTCCGCGACGACGAGt                                       >  2:364412/1‑137 (MQ=255)
                                                                                                  caacaCCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTAAGACGAGt                                       >  8:378611/1‑137 (MQ=255)
                                                                                                    accccaccGCGTACACGGGCGGGGCCGGCCGCGGGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTg                                     <  2:6068/134‑1 (MQ=255)
                                                                                                    acacCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTg                                     >  4:296370/1‑137 (MQ=255)
                                                                                                    acacCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGGTg                                     >  2:122648/1‑137 (MQ=255)
                                                                                                    acacCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCCGCGGCCAGGGCCGCCTCCGGGTGTTCAACGGCGTCTACGGGGTGCCGGTCTGTGCGCTCCGCTACGACGAGTTg                                     >  3:116536/1‑137 (MQ=255)
                                                                                                    acacCACCTCGGACAACGTCGCGGCCGGCCGCGCGGTGGCCGAACAGCTGGTGCGCGCCCCCGGCGGCAAGGGCCGCATCCTGGTGGTCCACCGCGTCTACGGGGTGCCGGTCTGTGGGCTCCGCACCGACGAGTTg                                     >  4:157415/1‑137 (MQ=255)
                                                                                                     caccacCTCGCCCAACGGCGCGCCGGGCGGCGCTTTGGCCTAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTga                                    <  5:410737/137‑1 (MQ=255)
                                                                                                         accTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACCCCGGCGGCAAGGGCCGCATCCGGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTgaagaa                                >  6:185922/1‑137 (MQ=255)
                                                                                                         accTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTgaagaa                                >  4:434526/1‑137 (MQ=255)
                                                                                                           cTCGGACAACGTCGCGGCCGGCCGCGCGGTGGCCGAACAGCTGGTGCGCGACACCGGCGGCCAGGGCCGCATCCGGGTGTTGAACGGCTTCTACGGGGGGCCGGTCTGTGCGCTCCGCGACGACGAGTTGAAGAAgg                              >  5:408730/1‑137 (MQ=255)
                                                                                                                aCAACGTCGCGGCCGGCCGCGCGGTGGCCGAACAGCTGGTGCGCGACCCCCGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGGGGCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTAATg                         >  7:10844/1‑137 (MQ=255)
                                                                                                                     ggcgcgGCCGGCCCCGCGTTGGCCGAACAGCTGGTGCGCGACCCCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTTTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGa                    <  7:467754/135‑1 (MQ=255)
                                                                                                                       cgcgGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCCTCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGCTCCGCTTCGACGAGTTGAAGAAGGTACTGGCCGatc                  >  3:179400/1‑137 (MQ=255)
                                                                                                                         gggccggccGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGatcat                <  2:319403/136‑1 (MQ=255)
                                                                                                                            ccggccgCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTGAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCg             >  4:219604/1‑137 (MQ=255)
                                                                                                                                   cgctTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGc      <  6:369214/137‑1 (MQ=255)
                                                                                                                                       ttGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCCGAt  <  6:195647/137‑1 (MQ=255)
                                                                                                                                       |                                                                                                                                        
CTCGGTACCCTGAGCGTGATCGACCCCTGGCTCAAGCGCATCCAGGCAGCAGGTATCCCGCTTTTCACCATTGATGTGCCATCGCAGTACAGCCTCAACAACACCACCTCGGACAACGTCGCGGCCGGCCGCGCGTTGGCCGAACAGCTGGTGCGCGACACCGGCGGCAAGGGCCGCATCCTGGTGTTCAACGGCTTCTACGGGGTGCCGGTCTGTGCGATCCGCTACGACGAGTTGAAGAAGGTACTGGCCGATCATCCGCAATTGCAGAT  >  KT2440/3134432‑3134703

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑