Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,440,3480TG53.6% ‑5.0 / 12.6 32A19A (GCT→GCG) CJ019_03266hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (12/1);  new base G (11/4);  total (27/5)
Fisher's exact test for biased strand distribution p-value = 3.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.21e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAA  >  KT2440/3440217‑3440473
                                                                                                                                   |                                                                                                                             
cAAACGTATCAATGGTGGTAGCAACGGTCTGCAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGTCCACCCGCCTGGTCGCCCTCGCGCTCCTGCTCGCCCCCCGAGCTggcgg                                                                                                                          >  5:433659/1‑137 (MQ=255)
 aaaCGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCGggcggc                                                                                                                         >  5:29492/1‑137 (MQ=255)
                 gtAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTggg                                                                                                         >  7:151016/1‑137 (MQ=255)
                    gCAACGGTCTGGAGGATCGCAAGGGCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCCGCCggggcgg                                                                                                      >  7:322181/1‑134 (MQ=255)
                    gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCTGGCGGCGCCCCGGCAGCCGGGgtgt                                                                                                      >  8:110884/1‑137 (MQ=255)
                    gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCGGGCAGCCAGGgtgg                                                                                                      >  4:29051/1‑136 (MQ=255)
                    gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCGGGCGGCGCCCGGGCAGCCTGGgggt                                                                                                      >  5:431393/1‑137 (MQ=255)
                              ggaggaTCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCAGCGCGGGCGGCGCCCGGGCCGCCGGGGGGTGGCAGGCCac                                                                                            >  1:412645/1‑136 (MQ=255)
                                aggaTCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGTGTGGGAGGCCaccc                                                                                          >  3:316495/1‑134 (MQ=255)
                                aggaTCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCCCCGGCGGCGCCCGCGCCGCCTGGGGGGGGCCCGCCaccc                                                                                          >  3:6388/1‑134 (MQ=255)
                                 ggaTCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCCGGCAGCCGGGGGGTGGCAGGCCCAccc                                                                                         >  4:369076/1‑135 (MQ=255)
                                       cAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCTGGCGGCGCCCGGGCAGCCGGGGTGGGGCAGGCCAACGCCTATaa                                                                                   >  4:178465/1‑137 (MQ=255)
                                       cAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCTGGCGGCGCCCCGGCAGCCTGGGTGTGGGAGGCCCACCCCGATaa                                                                                   >  4:328235/1‑137 (MQ=255)
                                       cAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGCGCCGCCTGGGGGTGGCAGGCCAACGCCAATaa                                                                                   >  5:176900/1‑137 (MQ=255)
                                       cAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCACCCCAGGCGGCGCCCGGGCCGCCTGGGTGTTGCACGCCAACGCCGATaa                                                                                   >  2:29800/1‑137 (MQ=255)
                                        aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGGCCGCGCGGGCGGGGCCCGGGCCGCCGGGGGGTGGGCGGCCAACGCCGATAAg                                                                                  >  3:409396/1‑137 (MQ=255)
                                        aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGTGTGGGAGGCCAACGCCGATaat                                                                                  >  3:111690/1‑136 (MQ=255)
                                            cccTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCGGGGGGTGGCCGGCCAACGCCGATAAGgcgc                                                                              >  6:235055/1‑137 (MQ=255)
                                                         agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCAGGCGGCGCCCCGGCCGCCTGGGTGTGGCAGGCCAACCCCGAAAAGGCGCAGCACGCTGATCa                                                                 >  4:148050/1‑137 (MQ=255)
                                                            gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCCGCGCAGGCGGCGCCCCGGCAGCCTGGGTGTGGCCGGCCAACGCCTATAAGGCGCAGCTAGCGTCTCAGGc                                                              >  8:431093/1‑137 (MQ=255)
                                                              tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCCGGCAGCCGGGGTGTGGCCGGCCCACGCCGATAAGGCGCAGCTCGCGGATCAGGCCg                                                            >  7:316595/1‑137 (MQ=255)
                                                              tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCCACGCCTATAAGGCGCAGCGCGCTGATCAGGCCg                                                            >  7:254904/1‑137 (MQ=255)
                                                                                 gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCTGGGGGTGGCAGGCCCACGCCTATAAGGCGCAGCGCGCGGATCAGGCCGATGACATCGGCAAGCAGtt                                         >  3:286308/1‑137 (MQ=255)
                                                                                 gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCGGGGTGGGGGAGGCCCACCCCTATAAGGCGCCGCGCGCTGCTCAGGCCGATGACGTGGGCcagcaggt                                         >  6:44030/1‑135 (MQ=255)
                                                                                      ggCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCCCGCGGGCGGCGCCCCGGCCGCCGGGGGGTGGGTGGCCCCCCCCGATAATGCGCCGCGGGCGGGGACGGCCGCTGGCCTCGGGAACGAGTTGGCgg                                    >  2:408477/1‑137 (MQ=255)
                                                                                                   gggggggTGGGGCGGCGGGTGGCCGCCTGGGCGGCGGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc                       <  1:36652/131‑1 (MQ=255)
                                                                                                   ggcggggTGGCGCTGGTGGGGCCCGCCCGGGGGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc                       <  7:82664/131‑1 (MQ=255)
                                                                                                   gccGCCGGGGCGCGGCGGCGGCCCCCCAGCGCGGGCGCCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc                       <  6:407758/135‑1 (MQ=255)
                                                                                                   gTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGACGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc                       <  8:208275/137‑1 (MQ=255)
                                                                                                      gCCGTGGCGCTGCTGCTGGGCGCCCGCGCGGGGGGCGGCCGGGGCGCCGGGGGGGGGGGGGCCACCGCCCCTAAGGCGCCGCGCGCGGGGCAGGGCGCGGCCATCGGCGAGCGGGTGGGGGCGAAGGGGGgcgcgcc                    >  2:332617/1‑136 (MQ=255)
                                                                                                      gCCGTGGCGCTGCTGCTGGCCCCCACCGCTGGCGGCGCCCGGGCAGCCTGGGTGGGGCACGCGAAAGCCGATAAGGCGCCGCGCGCTGATCAGGGCCATGACTTGGGGAAGCAGTTGGGGGAGAAGGATCgcgcgcc                    >  2:79156/1‑136 (MQ=255)
                                                                                                                        gccgccAGGGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGaa  <  7:177636/137‑1 (MQ=255)
                                                                                                                                   |                                                                                                                             
CAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAA  >  KT2440/3440217‑3440473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑