Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,350,3600TG31.7% 185.0 / 19.9 121R28R (CGT→CGG) CJ019_04090hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (20/62);  new base G (37/1);  total (58/63)
Fisher's exact test for biased strand distribution p-value = 2.86e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 124 total aligned reads displayed.

TCAGCGCGCGTATTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTACACGGATGTGTGGATCTGCGCCGATCCGCAAGGCCACCTGCAAGCCACC  >  KT2440/4350226‑4350495
                                                                                                                                      |                                                                                                                                          
 cAGCGCGCGTATTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGTGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑c                                                                                                                                        <  2:194282/137‑1 (MQ=255)
 cAGCGCGCGTATTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGCCCCGTCGGCGCTGGCGCGTCCGgttg                                                                                                                                         >  5:419073/1‑136 (MQ=255)
 cAGCGCGCGTATTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGtttg                                                                                                                                         >  2:81246/1‑136 (MQ=255)
 cAGCGCGCGTATTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑c                                                                                                                                        <  6:13041/137‑1 (MQ=255)
   gcgcgcGTATTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCCCTGGCGCGGCCGGTT‑GAt                                                                                                                                      >  7:250335/1‑137 (MQ=255)
    cgcgcgTATTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATc                                                                                                                                     <  1:211090/137‑1 (MQ=255)
    cgcgcgTATTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATc                                                                                                                                     <  7:441656/137‑1 (MQ=255)
      cgcgTATTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCCGTT‑CAtata                                                                                                                                   >  7:374345/1‑137 (MQ=255)
       gcgTATTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGTTT‑GATCTaa                                                                                                                                  >  1:32986/1‑136 (MQ=255)
         gTATTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCt                                                                                                                                <  4:44691/137‑1 (MQ=255)
               gccATTGAGCAGGGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑c                                                                                                                         <  3:321703/136‑1 (MQ=255)
                 cATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCGGGCGCGCCCGGTT‑TATCTAACTGGGTggcc                                                                                                                        >  3:218944/1‑134 (MQ=255)
                     gATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATgg                                                                                                                  <  1:76044/137‑1 (MQ=255)
                     caTCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATgg                                                                                                                  <  1:76048/136‑1 (MQ=255)
                      aTCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCGGGCGCGCCCGGTT‑AATCTACCTGGATG‑cccctggg                                                                                                                  >  5:314849/1‑131 (MQ=255)
                       tCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGcc                                                                                                                <  6:184687/137‑1 (MQ=255)
                       tCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGcc                                                                                                                <  3:436714/137‑1 (MQ=255)
                         aGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCGGGCGCGCCCGGTT‑GATCTACCGGGATG‑CCG‑ATGGccca                                                                                                              >  2:241062/1‑135 (MQ=255)
                            gACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGGGGGGGCGCCCGGTT‑GATATACCGGGAGGCCCA‑ATGGCCAACg                                                                                                            >  3:79160/1‑137 (MQ=255)
                            gACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGTGT‑GGTCTACCAGGATT‑GCG‑CTGGCCAACgg                                                                                                           >  7:112560/1‑137 (MQ=255)
                            gACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGGTT‑GCTGTTCCAGGCTG‑CCG‑CTGGGCAACgg                                                                                                           >  3:318279/1‑137 (MQ=255)
                            gACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCGGGCGCGGCCGGTT‑GATGTACCGGGCTT‑CCC‑AGGGGCAAccg                                                                                                           >  3:94810/1‑135 (MQ=255)
                            gACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCGGGCGCGCCCGGTT‑GATCTACCGGGATG‑CCG‑ATGGCCCACgc                                                                                                           >  2:10169/1‑136 (MQ=255)
                              ccTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCCCCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCGGGCGGGCCCCGTT‑GATCTAACAGGCTT‑CCG‑CTGGGCAACgggg                                                                                                         >  8:308364/1‑136 (MQ=255)
                              ccTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGGTT‑GATCTACCTGGATG‑CCG‑ATGGCCCACgggg                                                                                                         >  4:420808/1‑136 (MQ=255)
                                tGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTAc                                                                                                       <  8:102990/137‑1 (MQ=255)
                                tGATCGAGGCCCGCCGCCCATGCTTGACTGACCCCTGCCGCGCACCCTGCACTACGTTGCCTACAGTCTGCCGGGCCTGACCCGGCGGCGCTGTCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTAc                                                                                                       <  1:322123/137‑1 (MQ=255)
                                tGAGCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTAc                                                                                                       <  4:321712/137‑1 (MQ=255)
                                 gATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTAcg                                                                                                      <  7:170758/137‑1 (MQ=255)
                                   tCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGTTT‑GATATACCTGGATG‑CCG‑ATGGCCAACGGGTAcgcg                                                                                                    >  5:204336/1‑137 (MQ=255)
                                     gAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCGGGCGCGCCCCGTT‑CATCTACCTGGTTG‑CCG‑ATGGCCAACGGGTACGCGAc                                                                                                  >  4:183957/1‑137 (MQ=255)
                                     gAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCGGGCGCGACCGGTT‑GATATACCTTGCTT‑CCC‑ATTGCCAACCGGTAcgcgcc                                                                                                  >  8:19586/1‑135 (MQ=255)
                                      aGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACa                                                                                                 <  3:27032/137‑1 (MQ=255)
                                      aGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACa                                                                                                 <  8:329656/137‑1 (MQ=255)
                                       cgCCCGCCGCCCATGCATTACTCCCCCCTGCCGCGCACCCTCCACTACTTTGACGACAGTCAGCCGGGCGTGACCCGGCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑ACG‑ATGGCCAACGGTTACGCGACAg                                                                                                <  8:89368/136‑1 (MQ=255)
                                         cccGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCGGGGGCGCCCCGGT‑GATGTAGCAGGCTG‑GCG‑ATGGCCCACGCGGACGCGACcgcg                                                                                              >  2:417094/1‑137 (MQ=255)
                                          ccgccgCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCGGGCGCGCCCGGTT‑GATCTACCAGGATG‑CCG‑ATGGCCCACGGGTAAGCGCCCGCGa                                                                                             >  6:345335/1‑137 (MQ=255)
                                                      gttGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCTGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTgcgcg                                                                                 <  5:406378/136‑1 (MQ=255)
                                                      cTTTACGGCCCCCTGCCGCGCAGCCTGCACTACGTTGACGACAGTCAGCCGGGCATGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGCATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTgcgcg                                                                                 <  3:54670/137‑1 (MQ=255)
                                                      cTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTgcgcg                                                                                 <  2:213452/137‑1 (MQ=255)
                                                      cTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTgcgcg                                                                                 <  1:72409/137‑1 (MQ=255)
                                                      cTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGGCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTgcgcg                                                                                 <  6:388934/137‑1 (MQ=255)
                                                         gACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCAt                                                                              <  5:112872/137‑1 (MQ=255)
                                                          aCTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCAGGCGCGACCGGTT‑CATCTACCAGGATT‑GCG‑ATTGCCAACGGGTACGCCCCAGCGCAACCCTTTCGCGCaaa                                                                             >  6:434855/1‑135 (MQ=255)
                                                           cTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCGGGCGCGACCGGTT‑CGTCTGCAGGGAGG‑GCC‑CTGGGCAACCGTGACGGGTCACCGACACCCTTTCGCGGATcc                                                                            >  2:408605/1‑136 (MQ=255)
                                                             gCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGTTT‑CATCTACCAGGATG‑CCG‑ATTGCCAACGGGGACCCGCCCGCCAAACCCATGCGCGCATcgcc                                                                          >  5:236278/1‑137 (MQ=255)
                                                             gCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCAGGCGCCCACGTTT‑GTACTTACAGGATT‑GCG‑CTGGCCAACGGGTACGCGAGACCGAAACCCTATCGCGCAAcgcc                                                                          >  8:93161/1‑137 (MQ=255)
                                                              cccccTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGTTT‑CATCTACCGGGATG‑CCG‑AAGGCCCACGGGTAACCGACACCGAAACCCATGCCCGCAAcgccc                                                                         >  8:144113/1‑136 (MQ=255)
                                                                 ccTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGTTT‑CATATTCCTGGCTG‑CCG‑ATGGCCAACGGGTACGCGCCAGCGCAACCCTTGCGCGCATcgccgcct                                                                      >  8:350522/1‑135 (MQ=255)
                                                                 ccTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGGTT‑CATCGACCTGGCTG‑CCG‑ATGGCCCACGGGTACGCGACAGCGCAACCCTTGCGCGCATCGCCGCaa                                                                      >  6:215757/1‑136 (MQ=255)
                                                                 ccTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCGGGCGCGGCCGGTT‑GATCTTCCTGGCTG‑CCG‑CTGGCCCACGGGTACCCGCCCCCGCAACCCATGCGCGCCTCGCCCCaa                                                                      >  6:73211/1‑136 (MQ=255)
                                                                 ccTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCGGGCGCGCCCGGTT‑CATCTACCGGGATG‑CCG‑CTGGCCAACCGGTACGCGGCAGCGCAACCCTTGCGCGCATAGCCGCAt                                                                      >  4:228322/1‑137 (MQ=255)
                                                                    cccGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTgg                                                                   <  3:383279/136‑1 (MQ=255)
                                                                     ccGCGCACCCTGCACTACGTTTACGACTGTGTGCGGGGCATGACCCGTCGGGGCTGGCGCGACCGTTT‑CATCTACCTGGTTG‑CGG‑TTGGCCAAGGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGt                                                                  <  3:11634/137‑1 (MQ=255)
                                                                     ccGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGt                                                                  <  7:423309/137‑1 (MQ=255)
                                                                     ccGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGt                                                                  <  3:375100/137‑1 (MQ=255)
                                                                      cgcgCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCGGGCGCGCCCGTTT‑CATCTACCAGGATG‑CCC‑ATGGCCAACGGGTACGCGCCAGCGCAACCCCTGCGCGCCTCGCCGCATTgggg                                                                 >  8:359038/1‑135 (MQ=255)
                                                                         gcACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATc                                                              <  5:97622/137‑1 (MQ=255)
                                                                         gcACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGAGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATc                                                              <  8:297095/137‑1 (MQ=255)
                                                                         ccACCCGGCACTACGTTGACGCCATTCAGCCGGGCCTGCCCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATc                                                              <  6:51454/136‑1 (MQ=255)
                                                                          cACCCGGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATcc                                                             <  2:459079/137‑1 (MQ=255)
                                                                            cccTGCACTACGTTGACGACAGTCAGCCGTGCCTGACCCGGCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATcccc                                                           <  8:155070/137‑1 (MQ=255)
                                                                            cccTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCGGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATcccc                                                           <  2:375793/137‑1 (MQ=255)
                                                                             ccTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCCGTT‑GATGTAACAGGCTG‑ACG‑ATGGCCCCACGGTTCGCGCCAGCCAACACCATGCGCGCCACGCCGCCTTTGTGATccgcc                                                          >  8:398394/1‑137 (MQ=255)
                                                                              cTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATcccccc                                                         <  6:150918/137‑1 (MQ=255)
                                                                                gCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCgg                                                       <  1:155937/137‑1 (MQ=255)
                                                                                  aCTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGGGCAGGCGCCCCCGGTT‑GATATACCTGGGTG‑ACG‑ATGGCCAACGGGTACGCCCCACCCAAACCCCTTCGCGCCACCCCCCCTTGGTGGTACCCCCCGcc                                                     >  4:122533/1‑137 (MQ=255)
                                                                                  aCTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTAc                                                                                                       >  6:179944/1‑87 (MQ=255)
                                                                                  aCTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTAc                                                                                                       <  5:179944/87‑1 (MQ=255)
                                                                                  aCTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCAGGCCACAACAATT‑AAACTAACATGATT‑CCC‑CTGGCCAAACGGTACGCGCCCCCCCAAACCATTCGCGCAAACCCGCAATGGTGATCACCCCCGcc                                                     >  4:83712/1‑137 (MQ=255)
                                                                                   cTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCt                                                    <  7:99833/137‑1 (MQ=255)
                                                                                   atACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCt                                                    <  7:342661/136‑1 (MQ=255)
                                                                                    aaCGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTa                                                   <  1:443521/136‑1 (MQ=255)
                                                                                     aCGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGGTT‑AATCTACCAGGATG‑CCC‑AAGGCCAAACGGTAAGCCACACCCAAACCCATGCCCCCAAAGCCCCATTGGTGATACCCCCCGccgaa                                                  >  2:70843/1‑134 (MQ=255)
                                                                                      cGTTGACGACCGTCAGCCGGGCCGGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTaca                                                 <  1:206388/137‑1 (MQ=255)
                                                                                      cGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACGGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTaca                                                 <  7:29240/137‑1 (MQ=255)
                                                                                      cGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGGTT‑CATCTACCTGGAGG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTaca                                                 <  1:254841/137‑1 (MQ=255)
                                                                                       gTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTacac                                                <  7:242880/137‑1 (MQ=255)
                                                                                        ttGACGACAGTCAGCCGGGCCTGACCCGTCGGGCCGGGGGGGCCCGTGT‑CATCTACAGGGATG‑CCG‑ATTGCCCACCGGTTAGCGCAAGCGCAAACCGTGAGCGCAAAGCCCGCTTGGTGATACCCCCGGCCGAAACg                                               >  2:331573/1‑137 (MQ=255)
                                                                                        ttGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGGTT‑GATCTACCAGGATG‑CCG‑ATGGCCAACGGGTACGCGACCGCGAAACCCTTTCGCGCATCGCCGCATTGGTGATCCCCCCCGCCTaaacc                                               >  2:49503/1‑136 (MQ=255)
                                                                                         tGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGGTT‑GATCTACCTGGATG‑CCG‑ATGGCCCACGGGTACGCGCCAGCGCCACCCATGCGCGCCTCGCCGCATTGGTGATCCCCCCCGCCTACACgg                                              >  4:136774/1‑137 (MQ=255)
                                                                                          gacgacAGTCAGCCGGGCCTGACCCGTCGGCGCTGGGGGGTCCCTTT‑AATCTTCCAGGATT‑CCG‑ATTGGCAACGGGTACGCGCCACCGAAAACCGTGCGCGCATCGGCGGATTGGGGATGCCCCCCGCCTACACgca                                             >  2:299494/1‑135 (MQ=255)
                                                                                          gacgacAGTCAGCCGGGCCTGACCCGTCGGCGCGGGCGCGACCGGTT‑GATATAACTGGATG‑CCG‑ATGGCCAACCGGTAAGCGCCCCCGAAACCCATTCGCGCCTCGCCCCAATTGTGATACCCCCCGCCGACACCGa                                             >  4:216698/1‑137 (MQ=255)
                                                                                            cgacAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGGGT‑GATGTACCTGGCTG‑CCG‑CTGGCCCACGGGTTCGCGCCAGCGCAACCCGTGCGCGCATAGCCGCAATTGGGATCCCCCCCGCCTACCCGGAtg                                           >  6:316876/1‑137 (MQ=255)
                                                                                            cgacAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGGGT‑GATCTACCGGGATG‑CCG‑CTGGCCCACCGGTTCGCGCCCGCGAAACCCATGCGCGCCTCGCCGCCTTGGTGTTTCCCCCCGCCAACACCGAtt                                           >  4:68145/1‑136 (MQ=255)
                                                                                              acAGTCAGCCGGGCCTGACCCGTCGGGGCTGGCGGGCCCCGAT‑AATCGACCACGTAG‑CCC‑ATGGCCAACCGGGACCCCCCCACGCCAACCCTGCCCGCATCCCCGCCATGGTCATCCCCCCCCCCAACACCGAggtg                                         >  6:411274/1‑137 (MQ=255)
                                                                                              acAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGCCCGTTT‑CATCGACCTGGATG‑CCG‑CTGGCCAACGGGTACGCGCCAGCGAAACCCATGCGCGCCTCGCCGCATTGGTGATCCCCCCGGCCGACACGGAggtg                                         >  6:84343/1‑137 (MQ=255)
                                                                                              acAGTCAGCCGGGCCTGACCCGTCGGCGCGGGCGCGCCCGGTT‑GCTCTAACAGGGTG‑CCGCATGCCCACCGGG‑GCGCGCCCCCCCAACCCATGCGCGCCAAGCCGCCTTGGTGGTACCCCCCCCCCACACCGAggtt                                         >  8:56252/1‑136 (MQ=255)
                                                                                                aGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCACATCGCCGCATTGGTGATCCCCCCGGCCTACACGGAtgtgtg                                       <  6:457591/137‑1 (MQ=255)
                                                                                                 gTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGGCCGGTT‑AATATTCCTGGATG‑GCG‑ATGGGCAACCGGTGAGCGCCCGCGACACCCATGCGCGCATCGCCGCAGTTGGGGTGCCCCCGGGCGACACGCGTGTGTgg                                      >  4:323021/1‑137 (MQ=255)
                                                                                                     gCCGGGCCTGACCCGTCGGGGCGGGGGGGGCCGGTT‑TCTCGTCCAGGATG‑GCG‑CTGGGCCACGGGTGCGCGCCCGCGGAACCCCTGCGCGCCTCGCCCCCGTGGTGGTGCCCCCCGCCGACACCGATGTGTgggtgt                                  >  4:387436/1‑133 (MQ=255)
                                                                                                     gCCGGGCCTGACCCGTCGGCGCGGGCGCGACCGTTTAAAAAAAAATGAATA‑CC‑‑ATTGCCAAACGGTACGCGCCCGCGAAACCCTTGCCCGCAAAGCCGCCTTTGTGATGCCCCCCGCCAACACGGAAGTGTGGctct                                  >  4:357087/1‑137 (MQ=255)
                                                                                                        gggCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTACACGGATGTGTGGATCTgcg                               <  5:405804/137‑1 (MQ=255)
                                                                                                                   gTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTACACGGATGTGTGGATCTGCGCCGATCCGCaa                    <  1:241062/137‑1 (MQ=255)
                                                                                                                      ggCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTACACGGATGTGTGGATCTGCGCCGATCCGCAAGGc                 <  1:10169/137‑1 (MQ=255)
                                                                                                                            ggCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTACACGGATGTGTGGATCTGCGCCGATCCGCAAGGCCACCTg           <  8:374345/137‑1 (MQ=255)
                                                                                                                                 gACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTACACGGATGTGTGGATCTGCGCCGATCCGCAAGGCCACCTGCAAGc      <  5:73211/137‑1 (MQ=255)
                                                                                                                                 gACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTACACGGATGTGTGGATCTGCGCCGATCCGCAAGGCCACCTGCAAGc      <  5:434855/137‑1 (MQ=255)
                                                                                                                                 gACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTACACGGATGTGTGGATCTGCGCCGATCCGCAAGGCCACCTGCAAGc      <  4:224042/137‑1 (MQ=255)
                                                                                                                                 gACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACCGGTACGCGACAGCGCAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCAACACGGATGTGTGGATCTGCGCCGATCCGCAAGGCCACCTGCAAGc      >  8:445269/1‑137 (MQ=255)
                                                                                                                                      |                                                                                                                                          
TCAGCGCGCGTATTGTCCATTGATCAGTGACCTGATCGAGGCCCGCCGCCCATGCTTGACTGCCCCCTGCCGCGCACCCTGCACTACGTTGACGACAGTCAGCCGGGCCTGACCCGTCGGCGCTGGCGCGACCGTTT‑CATCTACCTGGATG‑CCG‑ATGGCCAACGGGTACGCGACAGCGAAACCCTTGCGCGCATCGCCGCATTGGTGATCCCCCCGGCCTACACGGATGTGTGGATCTGCGCCGATCCGCAAGGCCACCTGCAAGCCACC  >  KT2440/4350226‑4350495

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑