Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,384,2760CG35.0% 58.1 / 18.7 41intergenic (+539/‑319)CJ019_05064/CJ019_05067putative protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (2/24);  new base G (14/0);  total (17/24)
Fisher's exact test for biased strand distribution p-value = 5.17e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGA‑TTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCG  >  KT2440/5384163‑5384405
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aGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTc                                                                                                             <  7:166301/137‑1 (MQ=255)
  acacGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTGTGGCGGGAATTTGGGCGCGGGGGGGCgg                                                                                                           >  7:101465/1‑136 (MQ=255)
    acGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGaaa                                                                                                         <  5:49358/137‑1 (MQ=255)
      ggCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCGTTTTTTGTGGCTGGGATATGGGGGCGGGGGGGCggtaaa                                                                                                       >  5:136120/1‑132 (MQ=255)
       gCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGGCTGGAATTTTGGCGCTGCGGGTCGaaaaaa                                                                                                      >  8:48182/1‑136 (MQ=255)
       gCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCGTTTTTTTTGGCTGGCATTTTGGGGCTGGGGGGCGcaaaaa                                                                                                      >  7:409731/1‑136 (MQ=255)
         tAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGTTTTTTTGTGGCTGGAATTTGGGCGCTGCGGGGCGAAAAATCg                                                                                                    >  6:421481/1‑137 (MQ=255)
            gCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGGATTTGGGCGCTGCGGGTCGAAAAATCGGA‑t                                                                                                >  6:222388/1‑137 (MQ=255)
             cATGTAGTACCGACAGCGGAGAACTGGCGGACGGGGGTTCAAAACCCCCCGGCTCCACCAAATCCAAAGAAGAACACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGA‑tt                                                                                               <  1:292519/137‑1 (MQ=255)
              aTGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGA‑ttt                                                                                              <  2:295180/137‑1 (MQ=255)
                gtagtaCCTACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGA‑TTTaa                                                                                            <  8:95617/137‑1 (MQ=255)
                  agtaCCGACAGCGGAGTACTGGCGGAAGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCACTCGGGAGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGA‑TTTAAcc                                                                                          <  2:129073/137‑1 (MQ=255)
                   gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTGGGGGGTATTTGGGGGCGGCGGGGGGGAAAATCGGA‑TTTAAccc                                                                                         >  6:387393/1‑137 (MQ=255)
                   gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTGGCGGGTATTTGGGCGCGGGGGGTCGCAAAAACGGA‑TTTATccc                                                                                         >  6:111007/1‑137 (MQ=255)
                   gtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGGCTGGAATTTTGGCGCTGCGGGTCAAAAAATAGGA‑TTTAAacc                                                                                         >  2:263477/1‑137 (MQ=255)
                    taCCGACAGCGGAGTACTGGCGGTCGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCTGGTCGAAAAATCGGA‑TTTAAcccc                                                                                        <  3:420484/137‑1 (MQ=255)
                     aCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTGTGGTGAATTTGGGGGCTGCGGGGGGAAAAAAAGGATTTTAAcccc                                                                                        >  6:25782/1‑137 (MQ=255)
                     aCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTGTGGCTGGTATTTTGGCGCGGCGGGTCGAAAAATAGGA‑TTTAAaccca                                                                                       >  8:142088/1‑136 (MQ=255)
                     aCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCGTTTTTTTTTGCTGGAATTTGGGCGGTGGGGGGGGAAAAATAGGA‑TTTTAacccg                                                                                       >  5:182745/1‑136 (MQ=255)
                     aCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCGGTTTTTGTGGCTGGAATTTGGGGGCTGGGGGGGGCAATATCGGG‑TTTTAccccg                                                                                       >  8:367716/1‑136 (MQ=255)
                        gACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTGTTGCTGGAATTTTGGGGCTGCGGGTCGAAAAATAGGA‑TTTAACCCCtttt                                                                                    >  7:282840/1‑136 (MQ=255)
                          cAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGA‑TTTAACCCCTTTCtt                                                                                  <  1:22147/137‑1 (MQ=255)
                          cAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGA‑TTTAACCCCTTTCtt                                                                                  <  3:86908/137‑1 (MQ=255)
                          cAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGA‑TTTAACCCCTTTCtt                                                                                  <  3:441909/137‑1 (MQ=255)
                            gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTGTGGCGGGAATTTGGGGGCGGGGGGGGGATAAATAGGT‑TTTTACCCCcttctttt                                                                                >  2:272806/1‑135 (MQ=255)
                                aGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGA‑TTTAACCCCTTTCTTGTAggg                                                                            <  5:173582/137‑1 (MQ=255)
                                    cTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGA‑TTTAACCCCTTTCTTGTAGGGCGtt                                                                        <  7:199071/137‑1 (MQ=255)
                                    cTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGA‑TTTAACCCCTTTCTTGTAGGGCGtt                                                                        <  2:48578/137‑1 (MQ=255)
                                               gggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGGTTTTTGTGGCTGGAATTTGGGGGCTGCGGGGGGTAAAATAGGA‑TTTAACCCCTTTGTTTTTGGGGGTTTGCCCAAGATa                                                             >  6:467173/1‑137 (MQ=255)
                                                        tCCGCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGTCGCTGCGGGTCGAAAAATCGGA‑TTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTgcg                                                    <  8:13082/137‑1 (MQ=255)
                                                                    caccaAATCCAAAGAAGAAGACGCCCTAGGGCGTCTTTTTTTGTGCGTTGAAATTTGGGGCTGCGGGTCGAAAAATACGA‑ATTAAACCCAATCATATAGGGGGGATACCAAAGAATAAGCCAGCGCCGGTTTTACGt                                        >  6:188444/1‑137 (MQ=255)
                                                                         aaTCCAAAGAAGAAGACGCCCTAGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGA‑TTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCgg                                   <  6:212738/137‑1 (MQ=255)
                                                                                 gaagaagaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGA‑TTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt                           <  7:128819/137‑1 (MQ=255)
                                                                                 gaagaagaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGA‑TTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt                           <  8:337257/137‑1 (MQ=255)
                                                                                 caagaagaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGA‑TTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt                           <  8:395341/136‑1 (MQ=255)
                                                                                                gggCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGA‑TTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtct            <  3:239803/137‑1 (MQ=255)
                                                                                                gggCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGA‑TTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtct            <  8:423774/137‑1 (MQ=255)
                                                                                                gggCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGA‑TTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtct            <  5:111007/137‑1 (MQ=255)
                                                                                                 tgCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGA‑TTTCACCCCTTTCTTGTAGGGCGTTTCCCTAAGATTCTGCCTGAGCCTTTTTTCAGTTGCGGTCATTTTTTCAGGGTTTTAGtctc           <  2:73357/136‑1 (MQ=255)
                                                                                                 ggCGTCTTTTTTTGAGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGA‑TTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtctc           <  6:174017/137‑1 (MQ=255)
                                                                                                          ttttGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGA‑TTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCg  <  4:208377/137‑1 (MQ=255)
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AGACACGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGA‑TTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGTCG  >  KT2440/5384163‑5384405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑