Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,689,8240TG43.9% 36.8 / 14.9 58R238R (CGT→CGG) pyrC'Dihydroorotase‑like protein
Reads supporting (aligned to +/- strand):  ref base T (12/20);  new base G (5/20);  total (18/40)
Fisher's exact test for biased strand distribution p-value = 2.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCGCCGCTGCGC  >  KT2440/5689692‑5689959
                                                                                                                                    |                                                                                                                                       
aGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCCAGTAAGCCAGAAACCCAGCCCCCCTGgcc                                                                                                                                     >  3:157347/1‑136 (MQ=255)
aGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCCGATCACCAGCGCCCGTGgcg                                                                                                                                     >  2:360238/1‑137 (MQ=255)
     ccATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTAAGCCAGATCACCCGCGCCCGTGGCGCGCgg                                                                                                                                >  4:374562/1‑137 (MQ=255)
      cagggCCAGCTCCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAAAAGAGCGGCGGGCGGGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGc                                                                                                                               <  2:131827/134‑1 (MQ=255)
      cATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCACCGCCCGTGGCGCGCGGc                                                                                                                               >  7:313378/1‑137 (MQ=255)
           ccAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCGGAta                                                                                                                          >  5:24861/1‑136 (MQ=255)
           ccAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGACGCGCGGCTGATc                                                                                                                          <  3:154476/137‑1 (MQ=255)
           ccAGCTGCCTCGGCCTGCCGGGCACCCCGGACAGCGCCGAAACCGTGGCCCGGGCGCGCAACCTCCTGCGGGTGGAACAGAGCGGCGGGCGTCCGCACTTCAGCCAGAACACCAGCGCCCGTGGCGCGCGGCTGATc                                                                                                                          <  4:204072/137‑1 (MQ=255)
            cAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCCCCAGCGCCCGTGGCGCGGGGCTGATCg                                                                                                                         <  4:179588/137‑1 (MQ=255)
            cAGCTGCCTCGGCCTGCCGGGCAGCCCGGAGACCGCCTAAACCGTGGCCCTGGCGCGAAACCTGCTGCTGGTGGAACACAGCGGCGGGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCg                                                                                                                         <  5:469771/137‑1 (MQ=255)
                ttCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGTGGGCCCGTCAGCCCGCTAACCCCCGCCCGGGGGGCGCGGGGGGTCgcccc                                                                                                                     >  5:298107/1‑136 (MQ=255)
                  ccTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGTGGGCACTTCAGCCAGCTCACCCGCGCCCGGGGCGCGCGGCGGGTCgcccccc                                                                                                                   >  5:300005/1‑134 (MQ=255)
                  ccTCGGCCGGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCgcccagg                                                                                                                   <  2:367346/137‑1 (MQ=255)
                      ggCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTGAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGCTCgcccaggcccc                                                                                                               >  6:46847/1‑136 (MQ=255)
                             ggggCATCCCGGAGAGCGCCGAAACCGGGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGCGCGGCGTGCGGGCGCACTTCAGCCAAATCCCCAGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTg                                                                                                        <  8:456984/136‑1 (MQ=255)
                                    cccGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCGGATCGCCCAGGCCCAGGCCTTGGGCCTGc                                                                                                 >  2:89316/1‑137 (MQ=255)
                                         agagCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGGGGATCGCCCCGGCCCCGGCCGTGGGCCTGCCGGtt                                                                                            >  6:282620/1‑136 (MQ=255)
                                           cgggcCGAAACCGTGGCCCGGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGGGTGCGGGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                                          <  8:313378/134‑1 (MQ=255)
                                           cgcgcCGAAACCGGGGCCGTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGGGCGTGCGCACTTCAGCCAGATCACCAGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                                          <  5:100610/136‑1 (MQ=255)
                                           agCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                                          <  6:228452/137‑1 (MQ=255)
                                           agCCCCGAAACCTGGGCCGTGGGGCGCAACCTGATGCTGGTGGAACAGAGGGGCGTGCGTGCGCAGTTCAGCCAGATCACCAGCGCCCTTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                                          <  4:41455/137‑1 (MQ=255)
                                                cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACTTAGCGGGGTGGGTGCGCACTTCAGCCAGATCACCTGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                                                     <  5:371587/137‑1 (MQ=255)
                                                cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGGGTAACAGAGCGGCGGGCTGGCGCACTTCAGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                                                     <  4:172350/137‑1 (MQ=255)
                                                     cgggggCCCTGGCGCGCAACCTGCTGCTGGGGGAACAGAGCGGCGGGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                                <  6:300005/133‑1 (MQ=255)
                                                     cgggggCCCGGGCGGGCAACGGGGTGCTGGGGCAACAGCGCGGCGGGCGTGCGAACTGCCGCCACATCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                                <  2:164127/133‑1 (MQ=255)
                                                     ccGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                                <  7:6025/137‑1 (MQ=255)
                                                     ccGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                                <  6:237533/137‑1 (MQ=255)
                                                     ccGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGGGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                                <  4:123485/137‑1 (MQ=255)
                                                     ccGTGGCCCTGGCGCGCAACCTGCTGCTGGTGCAACCGAGCGGGGGGCGTGGGCACTTCAGCCAGATCCCCAGGGCCCGTGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                                <  5:46847/137‑1 (MQ=255)
                                                     ccGGGGCCCTGGCGCGAAACCTGCTGCGGGTGCAACAGGGCGGCGTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                                <  7:24515/137‑1 (MQ=255)
                                                          gCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGAGGGCGCACTTCAGCCAGATCACCAGCGCCCGAGGCGCGCGGCAGCTAGCCCAGGCCCCGCCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCt                                                                           >  2:140127/1‑137 (MQ=255)
                                                           cccTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGGGCGCCCGTAAGCCAGCTCACCAGCGCCCGTGGCGCGCGGCTGTTAGCCCCGGCCCACGGCGTGGGCCTGCCGGGAACCGCCGCTGTTgcgcgg                                                                          >  6:275891/1‑135 (MQ=255)
                                                           cccTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGGGGGCGCCCCTCAGCCACATAACCACCGCCCCGGGCGCGCGGCGGCTCGCCCCCGCCCCCGCCGGGGGGCGGCCGGGAACCCCCGCTGTAtcgcgg                                                                          >  2:463363/1‑135 (MQ=255)
                                                           cccGGGCGCGCAACCTGCTGCTGGGGCAACTGAGCGGCGTGCGTGCGAACTTCACCCACACCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTg                                                                          <  4:411408/137‑1 (MQ=255)
                                                                  gcgcAACGTGCTGCTGGGTGAACCGTGCGGGGTGCGTGCGCACTGCCGCCACAACCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGc                                                                   <  6:298107/137‑1 (MQ=255)
                                                                   cgcAACGTGGTGCTGGTGGAACAGCGCGGCGGGCGTGCGCACTTCAGCCAGAGCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCt                                                                  <  8:179794/137‑1 (MQ=255)
                                                                      aaCCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGAt                                                               >  3:226616/1‑137 (MQ=255)
                                                                              gctgGTGGAACAGAGCGGCGTGGGGGCGCACATGAGCCCGAACACCCGCCCCCGGGGCGCGCGGCGGCTCGCCCACGCCCCGGGCTTGGGCCTGCCGGTGACCGGCGCTGTAGGCCTGGACCCGCGTATGCACACgg                                                       >  4:239482/1‑137 (MQ=255)
                                                                                  gggcaccAGAGGGGCGGGGGGGCGCACTTCAGCCAGATCCCCAGGGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGa                                                   <  1:70007/131‑1 (MQ=255)
                                                                                         cgcgcggcgTGCGGGCGCACTTCCCCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTg                                            <  7:189035/134‑1 (MQ=255)
                                                                                           cgcggcgTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCGCGGACGAGTCGCTgcg                                          <  2:254195/136‑1 (MQ=255)
                                                                                            gcggcgTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcgc                                         <  6:434804/137‑1 (MQ=255)
                                                                                              ggcgTGCGTGCGCACGTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTTGGCCTGCCGGTAACCGCCGCTGTTGCGCTGTTCCAGCTGATACTAACGGACGAGTCGCTGcgcgg                                       >  8:59225/1‑136 (MQ=255)
                                                                                                ggtgcgtgcGCACTTCAGCCAGATCCCCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                                     <  4:423119/136‑1 (MQ=255)
                                                                                                ggtgcgtgcGCACTTCAGCCAGATCACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                                     <  1:360238/136‑1 (MQ=255)
                                                                                                ggtgcgggcGCACTTCACCCAGACCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                                     <  6:24861/136‑1 (MQ=255)
                                                                                                ggggcgggcgCACTTCAGCCAGATCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                                     <  7:178332/134‑1 (MQ=255)
                                                                                                cgtgcgtgCGCACTTCAGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                                     <  4:207116/137‑1 (MQ=255)
                                                                                                cgtgcgtgCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                                     <  4:226616/137‑1 (MQ=255)
                                                                                                     gtgcgCACTTCAGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTcc                                <  3:189111/137‑1 (MQ=255)
                                                                                                     gtgcgCACTGCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTcc                                <  8:216464/137‑1 (MQ=255)
                                                                                                     gggggaaCTTCAGCAACAACACCCCCGCGCGTGCCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTcc                                <  3:382840/131‑1 (MQ=255)
                                                                                                                       tCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAg              <  6:400718/137‑1 (MQ=255)
                                                                                                                          cccgcgcCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAgccg           <  5:334407/134‑1 (MQ=255)
                                                                                                                              cgcCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg                                          >  2:306796/1‑102 (MQ=255)
                                                                                                                              cgcCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg                                          <  1:306796/102‑1 (MQ=255)
                                                                                                                                 ccGGGGGGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCGCCGCTgc    <  2:90478/137‑1 (MQ=255)
                                                                                                                                   gTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCGGGACCCCGTGCAGCCGCCGCTgcgc  >  4:19089/1‑137 (MQ=255)
                                                                                                                                    |                                                                                                                                       
AGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCGCCGCTGCGC  >  KT2440/5689692‑5689959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑