Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,999,7510AC39.1% 52.4 / 13.4 46H260P (CAC→CCC) CJ019_05617putative protein
Reads supporting (aligned to +/- strand):  ref base A (6/22);  new base C (18/0);  total (24/22)
Fisher's exact test for biased strand distribution p-value = 5.03e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GTGGTGGCCTTCAAGGGCCCGCACATCAACCACCTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCT‑GCAGGAAACGGTCGCCTTCCGCGACCAACAAGGCCGCGAAGGCAGCCACACCGCACGCTTTGGCGAAATCGAGCAACGCGGCGCGGC  >  KT2440/5999618‑5999874
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gtggtgGCCTTCAAGGGCCCGCACATCAACCACCTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCAccc                                                                                                                           <  3:68147/137‑1 (MQ=255)
              gggCCCGCACATCAACCACCTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCCCCCGCCCCCCCCCCATACAGCGGCGcc                                                                                                             >  7:387004/1‑137 (MQ=255)
               ggCCCGCACATCAACCACCTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTTCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCa                                                                                                            <  2:322013/137‑1 (MQ=255)
                gCCCGCACATCAACCACCTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAg                                                                                                           <  1:187965/137‑1 (MQ=255)
                gCCCGCACATCAACCACCTGAACCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAg                                                                                                           <  1:426619/137‑1 (MQ=255)
                      acaTCAACCACCTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCCCCCGATCCTGCTGCGCCAGACCAGc                                                                                                     >  2:3815/1‑137 (MQ=255)
                            accaccTGACCCCGCGTACCCTGGATCTCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAg                                                                                               <  2:447605/137‑1 (MQ=255)
                            accaccTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAg                                                                                               <  1:175522/137‑1 (MQ=255)
                            accaccTGACCCCGCGTAACCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAg                                                                                               <  2:441187/137‑1 (MQ=255)
                             ccaccTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGAAACAGCAGCGCCACACCAGCGTAAAgg                                                                                              >  6:419877/1‑137 (MQ=255)
                                     ccccGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCCCCCGCCGCCCCCCCATACAGCAGCGCCAGCCCCGCTTAAACGCCCG‑GCac                                                                                     >  2:341654/1‑136 (MQ=255)
                                       ccGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCT‑GCAggc                                                                                   >  4:226421/1‑136 (MQ=255)
                                           gTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCCCCCCCCGCCCCCCCCTCCTGCTGCGCCAGGCCAGCTTCAAGGCCCG‑GCAGGAAAcc                                                                               >  2:84604/1‑136 (MQ=255)
                                           gTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCCCCCCCCGCCCCCCCATACAGCAGCGCCACACCCCCATCACGGCCCG‑GCAGGAAAcc                                                                               >  8:249652/1‑136 (MQ=255)
                                            tACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCT‑GCAGGAAACgg                                                                              <  4:371517/137‑1 (MQ=255)
                                             aCCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCCCCGCCACCCGATCCTGCTGCGCCACACCAGCATCAAGGCCCT‑GCAGGAAACGGt                                                                             >  5:446799/1‑137 (MQ=255)
                                                   gATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGGGGGTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCT‑GCAGGAAACGGTCGCCtt                                                                       <  5:92052/137‑1 (MQ=255)
                                                      atCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCCCCCCCCCGCCGCCCCCCCCTCCTGGGTGGCCACCCCCGCGTAAAAGCCCC‑GGAGGGAACGGTCGccgtacc                                                                    >  4:273287/1‑132 (MQ=255)
                                                      atCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCCCCCCCCCCCCCCCCCCCCATACAGCTGCGCCACACCCCCCTCAACGCCCA‑GCAGGCAACCGGCGccatacc                                                                    >  2:434826/1‑132 (MQ=255)
                                                       tCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCT‑GCAGGAAACGGTCGCCTTCcgc                                                                   <  2:221145/137‑1 (MQ=255)
                                                           tGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCCCCCGCCGCCACCCGATCCTGCTGCGCCAGCCCAGCTTCAAGGCCCT‑GCAGGAAACCGTCGCCTTCCGCGAcc                                                               >  2:38220/1‑137 (MQ=255)
                                                            gCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCCCCCGCCGCCACCCGCTACAGCTTCGCCACCCCCGCGTGAAGGCCCG‑GGACGAAACCGTCGCCGTCCCCGcccc                                                              >  4:30876/1‑136 (MQ=255)
                                                                tCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCCCCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCT‑GCAGGAAACGGTCGCCTTCCGCGCCccacaa                                                          >  3:450493/1‑137 (MQ=255)
                                                                    gCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCT‑GCAGGAAACGGTCGCCTTCCGCGACCAACAAGGcc                                                      <  7:449078/137‑1 (MQ=255)
                                                                     cTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCCCCCCCCCCCCCCCCAAACATCTGCGCCCGACCACCTTAAAGGCCCG‑GCCCGAAACCGTCGCCATACGCCACcaacaaagacc                                                     >  6:96516/1‑132 (MQ=255)
                                                                      tCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCT‑GCAGGAAACGGTCGCCTTCCGCGACCAACAAGGCcgc                                                    <  8:143145/137‑1 (MQ=255)
                                                                      gcGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCT‑GCCGGAAACGGTCGCCTTCCGCGACCAACAAGGCcgc                                                    <  6:388516/136‑1 (MQ=255)
                                                                      gcGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCT‑GCAGGAAACGGTCGCCTTCCGCGACCAACAAGGCcgc                                                    <  2:155744/136‑1 (MQ=255)
                                                                      gcGGCAGGCCTGCCAAGGGAAGCCCGCCGAAGGCCGTGGTTGACGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCT‑GCAGGAAACGGTCGCCTTCCGCGACCAACAAGGCcgc                                                    <  1:207082/136‑1 (MQ=255)
                                                                        ggCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCCCCCCCCCCCCCCCCATCCTGCAGCGCCAGCCCACCTTCAATGCCCG‑GCAGGAAACCGTCGCCTTCAGCGCCCAAAAAGGCCGCGa                                                  >  8:450168/1‑137 (MQ=255)
                                                                         gCATGTCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAACGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCT‑GCAGGAAACGGTCGCCTTCCGCGACCAACAAGGCCGCGaa                                                 <  6:386261/137‑1 (MQ=255)
                                                                           aTGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCT‑GCAGGAAACGGTCGCCTTCCGCGACCAACAAGGCCGCGAAgg                                               <  5:178606/137‑1 (MQ=255)
                                                                             gcctgccAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCCCCCCCCGCCCCCCGCTCCCGCGGGGCCCGACCAGCGTGAAGGCCCG‑GCAGGCAACGGTAGCCCTGCGCGGCCCACAAGGCCGCGGAgggc                                             >  4:35365/1‑135 (MQ=255)
                                                                             gcctgccAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCCCCCCCCCCCCCCCGATCCTGCTGCGCCCGACCAGCTTCAAGGCCCG‑GCAGGAAACGGTCGCCGTACGCGGCCAACAAGGCCGCGGAGGcc                                             >  3:133713/1‑136 (MQ=255)
                                                                             gcctgccAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCT‑GCAGGAAACGGTCGCCTTCCGCGACCAACAAGGCCGCGAAGGCa                                             <  8:462960/137‑1 (MQ=255)
                                                                                     aGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCCCCCCCCCCCCCCCCATCCAGCTGGGCCAGACCAGCGTCAAGGGCCG‑GGAGGGAACCGTCGCCGTCCGCGGCCACCAAGGGCGCGCAGGGCGCCAcccc                                     >  8:366521/1‑137 (MQ=255)
                                                                                        gCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCT‑GCAGGAAACGGTCGCCTTCCGCGACCAACAAGGCCGCGAAGGCAGCCACACCGCa                                  <  8:41403/137‑1 (MQ=255)
                                                                                         cATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCCCCGCCCCCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCG‑GCAGGAAACGGTCGCCTTCCGCGGCCAACAACGCCCCGCAGGCCGCCACACCGCAc                                 >  6:172526/1‑137 (MQ=255)
                                                                                          aTCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCCCCCGATCCAGCTGCGCCAGACCAGCGTCAAGGCCCT‑GCAGGGAACCGTCGCCGTCCGCGCCCCACAACGCCGCGCAGGCCGCCCCACCGCCCg                                >  6:218763/1‑137 (MQ=255)
                                                                                             ccgccgAAGGCCGTGGTTGAAGGCCCCCCCACCCCCCCCCCCCCGATCCTGCAGCGCCAGCCCCGGTTCAAGGCCCT‑GCAGGAAACCGTCGCCTTCCCCGCCCCACAATGCCGCGAAGGCCGCCACCCCGCACGCtt                             >  6:447765/1‑137 (MQ=255)
                                                                                              cgccgAAGGCCGTGGTTGAAGGCCCGCCCCCCCCCCCCCCCCCCATCCTGCTGCGCCAGCCCCGCTTCCAGGCCCT‑GCAGGCAACCGTCGCCATCCGCGACCAACACCGCCGCCCAGGCAGCCCCACCCCCCGCttt                            >  2:38254/1‑137 (MQ=255)
                                                                                              cgccgAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCAGCAGCGCCATTCCAGCATCAAGGGCATGGCGGGAAAC‑GTAGCCGTACGCGACCAACAAGGCCCCGAAGGCCGCCCCACCGCACCCttt                            >  8:378717/1‑137 (MQ=255)
                                                                                                       ccGTGGTTGAAGGCCCGCCCCCCCCCCCCCCCCCGATCCAGCGGCGCCAGCCCAGCGTCAAGGCCCT‑GGAGGAAACGGTAGCCGTACGCGCCCAACAACGCCGCGCACGCCGCCCCACCCCCCGCGTTGGCGAAAta                   >  8:373196/1‑136 (MQ=255)
                                                                                                            gTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCT‑GCAGGAAACGGTCGCCTTCCGCGACCAACAAGGCCGCGAAGGCAGCCACACCGCACGCTTTGGCGAAATCGAGCa              <  5:201332/137‑1 (MQ=255)
                                                                                                            gTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCT‑GCAGGAAACGGTCGCCTTCCGCGACCAACAAGGCCGCGAAGGCAGCCACACCGCACGCTTTGGCGAAATCGAGCa              <  5:194015/137‑1 (MQ=255)
                                                                                                                        cccACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCT‑GCAGGAAACGGTCGCCTTCCGCGACCAACAAGGCCGCGAAGGCAGCCACACCGCACGCTTTGGCGAAATCGAGCAACgcggcgcggc  <  8:387004/137‑1 (MQ=255)
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GTGGTGGCCTTCAAGGGCCCGCACATCAACCACCTGACCCCGCGTACCCTGGATATCGATGCAATCCAGCTCGGCATGCCTGCCAAGGGCATCCCGCCGAAGGCCGTGGTTGAAGGCCCGCCCACCCGCCGCCACCCGATCCTGCTGCGCCAGACCAGCTTCAAGGCCCT‑GCAGGAAACGGTCGCCTTCCGCGACCAACAAGGCCGCGAAGGCAGCCACACCGCACGCTTTGGCGAAATCGAGCAACGCGGCGCGGC  >  KT2440/5999618‑5999874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑