Predicted mutation
evidence seq id position mutation annotation gene description
RA KT2440 3,061,793 G→A S592N (AGC→AAC)  qedA_2 → Quinoprotein alcohol dehydrogenase (cytochromec)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,061,7930GA90.7% 125.5 / ‑4.9 46S592N (AGC→AAC) qedA_2Quinoprotein alcohol dehydrogenase (cytochromec)
Reads supporting (aligned to +/- strand):  ref base G (0/3);  major base A (20/19);  minor base C (0/4);  total (20/26)
Fisher's exact test for biased strand distribution p-value = 1.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAGCGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCAT  >  KT2440/3061659‑3061928
                                                                                                                                      |                                                                                                                                       
cGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAgcaaacggcaaacg                                                                                                                                       <  6:471656/137‑1 (MQ=255)
cGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAgcaaacggcaaacg                                                                                                                                       <  7:122127/137‑1 (MQ=255)
      ggTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGc                                                                                                                                 >  6:309907/1‑137 (MQ=255)
      ggTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGc                                                                                                                                 <  2:143777/137‑1 (MQ=255)
           tctcCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATaa                                                                                                                            <  7:1328/137‑1 (MQ=255)
           gctgcGGCTGGGGCGGCGCGGTGACGCTGTGGGGCGGCGAAGTGGCGAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATaa                                                                                                                            <  2:292237/133‑1 (MQ=255)
           gctcCGGCTGGGGCTGCTCTGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGTTCGGCATGCTCTTGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACTGCAAACGTCAAGCCATaa                                                                                                                            <  3:22498/136‑1 (MQ=255)
           gctcCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATaa                                                                                                                            <  5:285574/136‑1 (MQ=255)
           gctcCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATaa                                                                                                                            <  3:80681/136‑1 (MQ=255)
               cGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACgcc                                                                                                                        <  8:93251/137‑1 (MQ=255)
                        cggcgCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTg                                                                                                               <  8:273926/137‑1 (MQ=255)
                        cggcgCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTg                                                                                                               >  1:41611/1‑137 (MQ=255)
                              ggTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCtt                                                                                                         >  4:36660/1‑137 (MQ=255)
                                  ccGCGGTGGGGCGGCGTAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTtgctg                                                                                                     <  5:379837/137‑1 (MQ=255)
                                       gtgGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAGCGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCt                                                                                                <  8:205518/137‑1 (MQ=255)
                                       gtgGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAGCGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCt                                                                                                <  3:317505/137‑1 (MQ=255)
                                       gtgGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCt                                                                                                <  3:31576/137‑1 (MQ=255)
                                       gtgGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCt                                                                                                <  1:255201/137‑1 (MQ=255)
                                        tgGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTg                                                                                               >  8:269029/1‑137 (MQ=255)
                                             cggcgAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCgg                                                                                          >  6:427885/1‑137 (MQ=255)
                                                  atgTGGCAAAACGGGTAATGGACTTCAACCAGGGCGGAATGCGCTGGACATTAAAGTTGCCCACGCAGTTGCAGCAAAGGGCAACGGTCACGCCAAAACCCCTGCCTACTGCGGCTTGCTGCCGCTGATCGGTGGCa                                                                                     <  6:216734/135‑1 (MQ=255)
                                                  aaGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCa                                                                                     >  5:113067/1‑137 (MQ=255)
                                                  aaGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCa                                                                                     >  6:434303/1‑137 (MQ=255)
                                                    gTGGCCAAAGGGGTCAAGGACTTCAACCTGGGCGGCTTGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGAAAACGGCAAACGGCAAGCCATAACGCCTGCCTACTGCCGGTTGCTGCCGCTTATCGGTGGCAAg                                                                                   <  6:352171/137‑1 (MQ=255)
                                                     tGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGAAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGc                                                                                  >  7:131625/1‑137 (MQ=255)
                                                         cAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGAGCGGTGGCAAGCGGcc                                                                              <  7:303345/137‑1 (MQ=255)
                                                              gggTAAAGGACTAAAACCAGGGCGGCATGCTCTGGACCTTAAAGTTGCCCAACCTGTTGCACCAAACGCAAACCGTCACCCCAAACCGCCGGCCTACTGCCGCTTGCTGCCGCTGAGCGGTGCCACGGGGCCctact                                                                         <  4:240150/137‑1 (MQ=255)
                                                                  cAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCa                                                                     >  7:35396/1‑137 (MQ=255)
                                                                    aGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCaaa                                                                   >  5:284594/1‑137 (MQ=255)
                                                                             aCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACgagac                                                          >  3:240150/1‑136 (MQ=255)
                                                                             aCCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGagag                                                          >  1:318665/1‑137 (MQ=255)
                                                                                         tGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCaa                                              >  6:179738/1‑137 (MQ=255)
                                                                                               ggACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGc                                        >  2:42726/1‑137 (MQ=255)
                                                                                                  ccTACAAGTTGCCCAAGCAGTGGCACCAAACGGAAAACGTCACGCCATCACGCCTGCCTACTGCCGCTTGCTGCCGCTTAGCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCAtt                                     <  6:383458/137‑1 (MQ=255)
                                                                                                      aaaGTTGCCCAAGCAGTTGCCGCAAACGGCAAACGTCAAGCCATACCCCCTGCCTCCTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGcc                                 <  7:416116/136‑1 (MQ=255)
                                                                                                        aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGcccc                               >  2:419914/1‑136 (MQ=255)
                                                                                                        aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCg                               >  3:137224/1‑137 (MQ=255)
                                                                                                        aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCg                               >  5:465087/1‑137 (MQ=255)
                                                                                                        aGTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACGACCAAATGACGAGAGCCCCGCTGCCAACGAGGCATTCGCCCg                               >  7:464718/1‑137 (MQ=255)
                                                                                                         gTTGCCCAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCgg                              >  3:406912/1‑137 (MQ=255)
                                                                                                          ttccccACGCAGTTCCACCAACCGCCAACCGCCACCCCATACCGCCTGCCTCCTCCCGCTTGCTGCCGCTGAGCGGTGCCACGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGc                             <  3:165336/134‑1 (MQ=255)
                                                                                                            ccccAAGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACGGCCGCTTGCTGCCGCTGATCGGTGGCACGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCAc                           <  7:429451/136‑1 (MQ=255)
                                                                                                                aaGCAGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCgg                       >  8:415705/1‑137 (MQ=255)
                                                                                                                   catttGCAGCAAACGGCAAACGTCAACCCATACCGCCTGCCTACTGCCGCTTGCTGCCGCTTATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGc                    <  6:331331/134‑1 (MQ=255)
                                                                                                                    aGTTGCAGCAAACGGCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGct                   <  7:415705/137‑1 (MQ=255)
                                                                                                                          agcaAACGGCAACCGTCAAGCCATACCGCCTGCCTACTGCCGGTGGCGGCCGCTGTTCGGGGGCACGGGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTAc             <  8:75946/137‑1 (MQ=255)
                                                                                                                                 ggCAAACGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGt      <  2:342503/137‑1 (MQ=255)
                                                                                                                                     aGCGTCAAGCCATACCGCCTGCCTACTGCCGCTTGCTGCGGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCAt  <  7:77149/137‑1 (MQ=255)
                                                                                                                                      |                                                                                                                                       
CGTTTCGGTAGTCTCCGGCTGGGGCGGCGCGGTGCCGCTGTGGGGCGGCGAAGTGGCCAAACGGGTCAAGGACTTCAACCAGGGCGGCATGCTCTGGACCTTCAAGTTGCCCAAGCAGTTGCAGCAAACGGCAAGCGTCAAGCCATAACGCCTGCCTACTGCCGCTTGCTGCCGCTGATCGGTGGCAAGCGGCCCTACTACCAAATGACGAGAGCCCCGCTGCCAACGATGCATTCGCCCGGCACGCGGGGCTTCTTACCATCGGTGCAT  >  KT2440/3061659‑3061928

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑