| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 1,670,685 | A→C | 36.8% | intergenic (+22/‑109) | thrC_2 → / → CJ019_01569 | Threonine synthase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 1,670,685 | 0 | A | C | 36.8% | 21.8 / 2.5 | 38 | intergenic (+22/‑109) | thrC_2/CJ019_01569 | Threonine synthase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (20/2); new base C (12/3); total (32/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.77e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAGGGCTTTGTCAGCCA‑GCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCAGGAGCGGCTCATGCACCAGCC > KT2440/1670567‑1670804 | gTTCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGCCCTGAAGGCTGTACAGGGCTTTGTCAGCCA‑GCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTGGGGGCAGCCGCGCACCCCCCCCGtc > 1:133302/1‑135 (MQ=255) tCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAGGGCTTTGTCCGCCA‑GCACGGCAACCGCGGCACGCCGCTGTAATTGCATGGCGTTTTGGGCTGGCGCGCCCCCCCAAccga > 8:113149/1‑130 (MQ=255) tCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAGGGCTTTGTCAGCCA‑GCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTTGGGCTGCAGCGCAGCCCCCACggc > 3:261904/1‑131 (MQ=255) tCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAGGGCTTTGTCAGCCA‑GCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCCGCCCCACCGtctc > 7:235751/1‑135 (MQ=255) tCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAGGGCTTTGTCAGCCA‑GCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGtc > 8:103920/1‑133 (MQ=255) tCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAGGCCTTGGAC‑GCCACGCCCGGCAACCCCGGCAGGCCGGTGTAATTGCTTGCCGTTTGGGGGTGCAGCGCAGCCCCACCggggc > 1:49441/1‑131 (MQ=255) aGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAGGGCTTTGTCAGCCA‑GCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCTCAg > 7:188409/1‑135 (MQ=255) tGAAGAGCGTTGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAGGGCTTTGTCAGCCA‑GCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCCACGTCTCAGCCtt > 8:52320/1‑134 (MQ=255) agagCGTTGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAGGGCTTTGTCAGCCA‑GCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCCGCCCCCACCTCGCCGCCgtttac > 8:275166/1‑133 (MQ=255) agCGTTGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAGGGCTTTGTCAGCCA‑GCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGGCTCAGCCGTTTGcct > 3:53424/1‑135 (MQ=255) tGGCCAACGACCTGAAGGCTGTACAGGGCTTTGTCAGCCA‑GCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCCGCGCATCCCCAACGTCGCAGCCTTTTACCTCCGGGCGATCAGaaa > 8:286546/1‑135 (MQ=255) tGGCCAACGACCTGAAGGCTGTACAGGGCTTTGTCAGCCA‑GCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGaa > 1:49308/1‑134 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCA‑GCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTTGGGCTGCAGCGCAGCCCCAACGTCTCCTCCGTTTCCCACCTGGCTATCAGAAACAACCTAt > 1:231952/1‑133 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCA‑GCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCCGCGCCGCCCCCACGTCTCAGCCGTTTCCCTCCGGTCTTTACGAAACCTCCTAt > 7:134269/1‑133 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCA‑GCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCCGCGCCGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTc > 3:149628/1‑135 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCA‑GCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCCGCGCAGCCCCCACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTAt > 2:213115/1‑133 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCA‑GCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCCGCGCAGCCCCCACGTCTCAGCCTTTTACCGACTGTCTATAAGAAACCTCCTAt > 1:66147/1‑133 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCA‑GCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCCGCGCAGCCCCAACGGCTCCGCCTTTTCCCTCCTGTCTATCAGCAACATCCTAt > 8:195277/1‑133 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCA‑GCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCCGCGCAGCCCCAACGGCTCACCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTc > 1:157814/1‑135 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCA‑GCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCCGCCCCCACGTCGCAGCCGTTTCCCTCCTGTGGATCAGAAACATCCTATTc > 5:107052/1‑135 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCA‑GCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCCGCCCCAACCTCTCCGCCGTTTACCTCCAGTCTATCAGAAACATACTAATc > 6:251776/1‑135 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCA‑GCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCTCATCCTTTTACCTCCAGTGTATCAGAAACATCCTAtta > 6:35543/1‑134 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCA‑GCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTAt > 8:171614/1‑133 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCA‑GCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTc > 4:22003/1‑135 (MQ=255) ccTGAAGGCTGTACAGGGCTTTGTCAGCCA‑GCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCAAt > 4:112851/1‑133 (MQ=255) aGGCTGTACAGGGCTTTGTCAGCCA‑GCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCt > 4:253346/1‑135 (MQ=255) gCTGTACCGGGCTTTGGCACCCA‑GCACGGAACCCGCGCAACGCCGGTTTAATTGCAGGGGGTTGGGGGCGGCCGCGCACCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGc < 8:134269/135‑1 (MQ=255) aGGGCTTTGTCAGCCA‑GCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCCGCGCCGCCCCAACGTCTCCGCCTTTTCCCTCCGGTCTATCAGCAACATCCTATTCAGCCGGCCGGgcgc > 6:166431/1‑135 (MQ=255) tttGTCAGCCA‑GCACGGCAACCGGGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGc > 4:122880/1‑135 (MQ=255) tttGTCAGCCA‑GCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCCGCGCAGCCCCCACGTCTCAGCCGTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGc > 5:53671/1‑135 (MQ=255) cagcca‑gcACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCCGCCCCCACGGGTCCGGCGTTTGCCGCCGGTGTATCAGAAACCGACGATTCCACCTGCCGGGCGCCAAGccaaaa > 2:265718/1‑130 (MQ=255) cagcca‑gcACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCGGGCGCGCCGCCCCCACGGGTCAGCCGTTTTCCGCCCGTGTTTTCGAAAAAACCGATTACGGCCGCCGGGGGCCCAGCAaaaa > 5:254270/1‑135 (MQ=255) a‑gcACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCTCCGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCCAGCATAAATTAGc > 4:214215/1‑135 (MQ=255) cGGCACCCGCGGCAAGCCGGTGTAATTGCTGGGCGTTGGGGGCGGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGt < 8:235751/135‑1 (MQ=255) aCCGCGTCAAGCGGGTTTAATTGATTGGCTTTGGGGGCTGCTGCGAAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGTtcctcc < 4:109735/135‑1 (MQ=255) gcggTAATGGCAGGGCGTGTGGGGCGGCAGCGCAGCCCCCACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCAGGAGCGGc < 8:251846/132‑1 (MQ=255) aTTGCCTGGGGTTTGGGGCTGCCGCGCTGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCAGGAGCGGCTCATg < 8:98963/134‑1 (MQ=255) ggttttGGGGCGGCCGCGCTCCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCAGGAGCGGCTCATGCACCAGcc < 7:103920/131‑1 (MQ=255) | GTTCAGCCGTGAAGAGCGTTGCACGGTCCTGGCCAACGACCTGAAGGCTGTACAGGGCTTTGTCAGCCA‑GCACGGCAACCGCGGCAAGCCGCTGTAATTGCATGGCGTTTGGGGCTGCAGCGCAGCCCCAACGTCTCAGCCTTTTCCCTCCTGTCTATCAGAAACATCCTATTCAGCCTGCCGGGCGCCAAGCATAAATTAGCCGGTCCTCCCATCAGGAGCGGCTCATGCACCAGCC > KT2440/1670567‑1670804 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |