| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 3,072,630 | T→G | 28.3% | intergenic (‑308/+126) | CJ019_02884 ← / ← yabJ_2 | putative protein/2‑iminobutanoate/2‑iminopropanoate deaminase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 3,072,630 | 0 | T | G | 28.3% | 55.5 / 2.4 | 41 | intergenic (‑308/+126) | CJ019_02884/yabJ_2 | putative protein/2‑iminobutanoate/2‑iminopropanoate deaminase |
| Reads supporting (aligned to +/- strand): ref base T (10/17); new base G (11/2); total (22/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.90e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTTCTGGGGATGCAGGGAATGTGAGGGGCACACCCGATCTGTGCTGCCTGGCCGCAAGGGCCGGCGCATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTG‑AACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGACGGGCGCCTTCAGGGCGTCAG > KT2440/3072498‑3072759 | gTTCTGGGGATGCAGGGAATGTGAGGGGCACACCCGATCTGTGCTGCCTGGCCGCAAGGGCCGGCGCATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTG‑AACCGAAAAGGGGCGCAGGCCGGTGCCAgg > 8:97363/1‑135 (MQ=255) ggggATGCAGGGAATGTGAGGGGCACACCCGATCTGTGCTGCCTGGCCGCAAGGGCCGGCGCATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGGGGTG‑AGGCGCAAAGGGGCGCACGCCGGTGGCGggggggc > 2:293001/1‑134 (MQ=255) agtgAATGTGAGGGGCACACCCGATCTGTGCTGCCTGGCCGCAAGGGCCGGCGCATCAAAACAGGGAGCTTTTGACCCGGGCGCGGGTTTTG‑AACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGaaaaa < 3:160253/130‑1 (MQ=255) aaTGTGAGGGGCACACCCGATCTGTGCTGCCTGGCCGCAAGGGCCGGCGCATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTG‑AACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGt < 8:296127/135‑1 (MQ=255) ggggAGGGGCACACCCGAGCTGTGCTGCCTGGCCGCAAGGGCCGGCGCATCAAAAACGGGCGCTTTTGACCCGGGCGCTGGTTTTG‑AACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTAt < 6:163913/132‑1 (MQ=255) cacCCGATCTGTGCTGCCTGGCCGCAAGGGCCGGCGCATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGGTTTG‑AACCGAAATGGGGCGCAGGCCGGTGCCGGGCGGGAGAAAAAGGGAGTATTCCGGGTGCAt > 4:191194/1‑135 (MQ=255) cacCCGATCTGTGCTGCCTGGCCGCAAGGGCCGGCGCATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGGTTTG‑AACCGAAATGGGGCGCAGGCCCGGGGCGGGGGGGAGCAAAAGGGAGTATTACGGGTGCAt > 4:170967/1‑135 (MQ=255) acCCGATCTGTGCTGCCTGGCCGCAAGGGCCGGCGCATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTG‑AACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATg < 8:221367/135‑1 (MQ=255) gtgCTGCCTGGCCGCAAGGGCCGGCGCATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGGTTTGTAACCAAAAT‑GGGCGCCGGCCGGTGCCGGGCGGGTGAAAAAGGGAGTATTGAGGGTGCATTACGTggggg > 4:185151/1‑134 (MQ=255) gctgcCTGGCCGCAAGGTCCGGCGCATC‑AATAAGGGCGCTTTTGACCCGGGCGCGGGTTTTG‑AACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGa < 4:90392/135‑1 (MQ=255) gctgcCTGGCCGCAAGGGCCGGCGCATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTG‑AACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTgggg < 4:21947/132‑1 (MQ=255) gctgcCTGGCCGCAAGGGCCGGCGCATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTG‑AACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCg < 6:208093/134‑1 (MQ=255) ccTGGCCGCAAGGGCCGGCGCATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGGTTTG‑CCCCGCAATGGGGCGCCGGCCGGTGCGGGGCGGGTGAAAAAGGGAGTATTCCGGGTGCTTGAcggggggcgggggg > 1:163858/1‑135 (MQ=255) tGGCCGCAAGGGCCGGCGCATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTG‑AACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAggggg < 7:113952/135‑1 (MQ=255) ggCCGCAAGGGCCGGCGCATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTGTTG‑GACCGAAATGGGGCGCCGGCCGGTGCCGGGGGGGAGAAAAAGGGAGTATTGCGGGTGCATGCCGTGGGGGGGAGGGGgc > 1:172694/1‑135 (MQ=255) ggCCGCAAGGGCCGGCGCATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGGTTTG‑ACCCGATAGGGGGCACAGGCCGGTGCCGGGCGGGGGAACAACGGAGTATTCCGGGTGGATGCCGGGGGGCgggggggg > 1:172689/1‑134 (MQ=255) ggCCGCAAGGGCCGGCGCATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGGGTTG‑AGCCCCAATGGGGCGCCGGCCGGGGCCGGGCGGGAGCAAAAGGGAGGTTTCCGGGGGCCAGACGGGGGGCGGCgggg > 3:23612/1‑133 (MQ=255) cAAGGGCCGGCGCATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGGTTTG‑TACCGCAATGGGGCGCCGGCCGGTGCCGGGGGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGGGGGGCGGAGGGGgcatgca > 5:132588/1‑135 (MQ=255) gCCGGCGCATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGGTTTG‑GACCGGAATGGGGCGCCGGGCGGGGCCGGGCGGGGGCAAAAGGGAGTTTTCCGGGTGCATTCCGGGGGGGGGGGGGGggctgcatagcg > 8:132628/1‑131 (MQ=255) gcgcATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTG‑AACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGGCGTGGGGCGGAGGGGGCATGCATCGAGcgcc > 4:188524/1‑135 (MQ=255) gcATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTG‑AACCGAAATGGGGCGCAGGGCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCCTGACGTGGGGCGGAGGGGGCATGCATCGAGcgccgc > 6:252772/1‑135 (MQ=255) aTCAAAACCGGGCGCTTTGGCCCCGGGCGCGGGTTTTG‑ACCCGAAAGGGGGCGCCGGCCGGGGCCGGGCGGGAGAAAACGGGAGAATTCCGGGGGCATGACGCGGGGCGGAGGGGGCATGCAGCGAGCGCcgcgc < 2:299980/135‑1 (MQ=255) aTCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTG‑AACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCcgcgc < 7:97363/135‑1 (MQ=255) aTCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTG‑AACCGAAATGGGGCGCAGGCCGGTGCCGGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCcgcgc < 3:191194/135‑1 (MQ=255) aaaaGGGCGCTTTTGACCCGGGCGCGGGTTTTG‑CACCGAAATGGGGCGCCGGCCGGGGGCTGGGGGGAGGAAAACGGAGGATTCCGGGTGGATGACGGGGGGCGCAGGGGGCCTGCATACAGCGCCGCccgggcg > 5:85109/1‑135 (MQ=255) aaaaGGGCGCTTTTGACCCGGGCGCGGGTTTTG‑AACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGCCGTGGGGCGGAGGGGGCATGCCTCGAGCGCCGCgcgggcg > 6:117919/1‑135 (MQ=255) aaaaGGGCGCTTTTGACCCGGGCGCGGGGTTTT‑AAACGAAATGGGGCGCAGGCCGGTGGCGGGCGGGAGAAAAACGGAGTATTACGGGTGCATGACGTGGGGCGGCGGGGGCATGCATCGAGCGCCGCgcgggcg > 5:254221/1‑135 (MQ=255) gcTTTTGACCCGGGCGCGGGTTTTG‑AACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGgaga < 7:234053/62‑1 (MQ=255) gcTTTTGACCCGGGCGCGGGTTTTG‑AACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGgaga > 8:234053/1‑62 (MQ=255) ttttGACCCGGGCGCGGGTTTTG‑AACCGAAATGGGGCGCAGGCCGGTGCCTgg < 7:245867/53‑1 (MQ=255) ttttGACCCGGGCGCGGGTTTTG‑AACCGAAATGGGGCGCAGGCCGGTGCCTgg > 8:245867/1‑53 (MQ=255) tGACCCGGGCGCGGGTTTTG‑AACCGAAAGGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTg < 5:17239/133‑1 (MQ=255) tGACCCGGGCGCGGGGTTTG‑AACCGAAATTGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATTCATCGAGCGCCGCGCGGGCGGCACTCGATTgcg > 3:101975/1‑135 (MQ=255) gccccGGGCGCGGGTGTTG‑AACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAACAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTgcg < 3:185151/132‑1 (MQ=255) gACCCGGGCGCGGGTTTTG‑AACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTgcg < 6:132588/134‑1 (MQ=255) aCCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTgaga > 7:136818/1‑135 (MQ=255) aTGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGACGGgcg > 3:142486/1‑135 (MQ=255) gCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGAc < 6:293854/119‑1 (MQ=255) gCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGAc > 5:293854/1‑119 (MQ=255) gTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGACGGGCGCCTTCAGGGCGTCag < 6:115965/135‑1 (MQ=255) gTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGGGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGACGGGCGCCTTCAGGGCGTCag < 5:206251/135‑1 (MQ=255) | GTTCTGGGGATGCAGGGAATGTGAGGGGCACACCCGATCTGTGCTGCCTGGCCGCAAGGGCCGGCGCATCAAAAAAGGGCGCTTTTGACCCGGGCGCGGGTTTTG‑AACCGAAATGGGGCGCAGGCCGGTGCCTGGCGGGAGAAAAAGGGAGTATTCCGGGTGCATGACGTGGGGCGGAGGGGGCATGCATCGAGCGCCGCGCGGGCGGCACTCGATTGCGCGAACGCTGAATATCGTGAGACGGGCGCCTTCAGGGCGTCAG > KT2440/3072498‑3072759 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |