Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	KT2440	4,077,164	T→G	37.7%	L173R (CTG→CGG)	artJ_2 →	ABC transporter arginine‑binding protein 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	4,077,164	0	T	G	37.7%	18.9 / 5.3	45	L173R (CTG→CGG)	artJ_2	ABC transporter arginine‑binding protein 1
Reads supporting (aligned to +/- strand):  ref base T (23/2);  new base G (14/2);  total (41/4)
Fisher's exact test for biased strand distribution p-value = 6.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTACACCGTCGGTGCGCAGTCTTCCTCTACCCAGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCC‑GCCTGGACGGCGTGATTGCCGACAAATTCCCGCTGCACGAGTGGATGAACAAGAACGGCGGGGATTGCTGCAAGATCCTTGGCGACGTGGCGGACACCAAGGCCGATGCCGCCATCGCTGTGCGCAAGGACGACG  >  KT2440/4077032‑4077295                                                                                                                                       |                                                                                                                                    gCTACACCGTCGGTGCGCAGTCTTCCTCTACCCAGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCC‑GCCTgg                                                                                                                                   >  3:27064/1‑135 (MQ=255)      acCGTCGGTGCGCAGTCTTCCTCTACCCAGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCC‑GCCTGGACGGc                                                                                                                              >  4:51573/1‑135 (MQ=255)             gTGCGCAGTCTTCCTCTACCCAGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCC‑GCCTGGCCGGCGTGATTg                                                                                                                       >  5:137420/1‑135 (MQ=255)             gTGCGCAGTCTTCCTCTACCCAGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCC‑GCCTGGACGGCGTGCTTg                                                                                                                       >  4:61216/1‑135 (MQ=255)             gTGCGCAGTCTTCCTCTACCCAGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCC‑GCCTGGACGGCGGGATTg                                                                                                                       >  6:144726/1‑135 (MQ=255)                           tctACCCAGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCCGGGCC‑CCCGGGACGGCGTGATTGCCGACAAATTccc                                                                                                          >  1:96878/1‑135 (MQ=255)                           tctACCCAGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCC‑GCCGGGACGGCGTGATTGCCGCCAAATTCCCg                                                                                                         >  8:67307/1‑135 (MQ=255)                           tctACCCAGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCC‑GCCAGGCCGGCGGGCTTGCCCACAAAATACCg                                                                                                         >  2:291011/1‑135 (MQ=255)                                  aGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCC‑GCCTGGCCGGCGTGCTTGCCGACAAATTCCCGCTGcc                                                                                                    >  8:65448/1‑132 (MQ=255)                                  aGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCC‑GCCGGGGCGGCGGGATTGGCGACAAATTACCGCTGCa                                                                                                    >  7:147576/1‑133 (MQ=255)                                  aGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCC‑CCCGGGGCCGCGGGCTTGGCGGAAAAATACCGCGGCa                                                                                                    >  7:165611/1‑133 (MQ=255)                                  aGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCC‑CCCGGGCCGGCGTGATTGCCGCCAAATTACCGCGGCa                                                                                                    >  5:152416/1‑133 (MQ=255)                                       aTCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCC‑GCCTGGACGGCGGGATTTCCGACAAATTCCCGCTGCACGAGTgg                                                                                             >  2:45613/1‑135 (MQ=255)                                          tACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCC‑GCCGGGCCGGCGTGATTGCCGCCCAATTCCCGCTGCACGAGTGGAt                                                                                           >  3:187795/1‑134 (MQ=255)                                            cGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCACCGCAGACCTGGCGGCC‑GGCC‑CCCGGGCCGGCCGGGGTGCCCACGAAATGCCGCAGCCCGACTGGATGac                                                                                        >  1:267665/1‑134 (MQ=255)                                            cGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGCCCTGGCGGCC‑GGCC‑GCCGGGACGGCGTGATTGCCGACAAATTCCCGCTGCCCGAGTGGCTGa                                                                                         >  1:93571/1‑134 (MQ=255)                                                 aaGACGTGTACTGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCACCGCAGACCTGGCGGCC‑TGCA‑GCCTGGACGGCGAGAGTGCCGACAACTTCCCGCTGCAGGAGTGGATGAACAAGa                                                                                   >  4:55828/1‑135 (MQ=255)                                                 aaGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCC‑GCCGGGCCGGCGGGCTTGGCGCCAAATTACCGCGGCCCGAGGGGATGAACAAgc                                                                                   >  7:235698/1‑134 (MQ=255)                                                    aCGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCC‑GCCTGGACGGCGTGATTGCCGACAAATTCCCGCTGCACGAGTGGATGAACAAGAAcg                                                                                >  2:248931/1‑135 (MQ=255)                                                    aCGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑CGCC‑GCCAGGCCCGCGTTATTGGCGACAAATTCCCGCAGCCCCAGTGGCTGAAAAAGAAcg                                                                                >  3:23705/1‑135 (MQ=255)                                                          aCGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCC‑GCCTGGGCGGGGTGATTGGCGTCAAATTACCGGGGCACGAGGGGAGGGACCAGTACGgggggg                                                                          >  1:238429/1‑135 (MQ=255)                                                          aCGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCC‑GCCGGGCCGGCGGGATTGCCGCCCAATTCCCGCGGCCCGAGTGGCTGAACAAGAACGGCgggg                                                                          >  6:63563/1‑135 (MQ=255)                                                            ggCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCC‑GCCTGGCCGGCGTGATTGCCGCCAAATTACCGCTGCACGAGTGGATGAACAAGCACGGCgggggt                                                                        >  7:143402/1‑133 (MQ=255)                                                            ggCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCC‑GCCGGGACGGCGTGATTGCCGCCAAATTCCCGCTGCCCGAGGGGATGAACAAGAACGGCGGGGAt                                                                        >  7:94880/1‑135 (MQ=255)                                                                    cGGTGCCGACGTGATACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCC‑GCCGGGCCGGCGTGCGTTCCGACAAATTACCGCTGCACGAGTGGATGAAAAACAACGGCGGGGCTtgctg                                                                   >  7:168973/1‑132 (MQ=255)                                                                         ccGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCC‑GCCTGGACGGCGGGATTGCCCACAAATTACCGCTGCACGAGTGGATGAACAAGAACGGCGGGGATTGCTGCAAGATcc                                                           >  6:88342/1‑135 (MQ=255)                                                                               tGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCC‑GCCTGGCCGGCGTGATTGCCGACAAATTCCCGCTGCACGAGTGGATGAACAAGAACGGCGGGGATTGCTGCAAGATCCTTGGCg                                                     >  7:240674/1‑135 (MQ=255)                                                                                gAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCC‑GCCTGGCCGGCGTGATTGCCGACAAATTACCGCTGCACGAGTGGATGAACAAGAACGGCGGGGGTTGCTGCAAGATCCTTGGCGa                                                    >  5:82632/1‑135 (MQ=255)                                                                                    gtgaccccGACCATGCACCACCCCAACGCAGCCCGGGCGGCC‑GGCC‑GCCGGGACGGCGTGATTGCCGACAAATTCCCGCTGCACGAGTGGATGAACAAGAACGGCGGGGATTGCTGCAAGATCCTTGGCGACGTg                                                <  8:240674/130‑1 (MQ=255)                                                                                    cTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCC‑GCCTGGACGGCGTGATTGCCGACAAATTCCCGCTGCACGAGTGGATGAACAAGAACGGCGGGGATTGCTGCAAGATCCTTGGCGACGTg                                                >  7:174481/1‑135 (MQ=255)                                                                                          ccGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCC‑GCCTGGCCGGCGGGATTGCCGACCAATTACCGCTGCCCGAGTGGATGAACAAGAACGGCGGGGCTTGCTGCAAGGTCCTTGGCGACGTGGCGGac                                          >  1:72520/1‑135 (MQ=255)                                                                                          ccGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCC‑GCCGGGCCCGCGGGATTTGCGCCAAAATACCGCGGCCCGCGTGGATGCACAAGAACGGCGGGGGTTGCTGCAAGATCCTTGGCGGCGTGGCgggc                                          >  5:296904/1‑133 (MQ=255)                                                                                               cATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCC‑GCCTGGCCGGCGTGATTGCCGCCAAATTCCCGCGGCCCGAGTGGGTGTACAAGAACGGCGGGGATTGCTGGAAGATCCTTGGCGACCTGGCGGCCACCaa                                     >  1:3106/1‑135 (MQ=255)                                                                                                    aCGAAGCCAACGCAGACCTGGCGGCC‑GGCC‑GCCCGGACGGCGTGCTTTCCGACAAATTCCCGCAGCCCGAGTGGATGAACAAGAACGGCGGGGATTGCGTCCACATCCTTGGCGACGTGGCGGACACCAAGGCCg                                >  7:207247/1‑135 (MQ=255)                                                                                                    aCGAAGCCAACGCAGACCTGGCGGCC‑GGCC‑CCCTGGACCGCGTGATTGGCGACAAAATACCGCTGCACGAGTGGATGAACAAGAACGGCGGGGATTGCTGCAAGATACTTGGCGACGTGGCGGACACCAAGGcc                                 >  7:82492/1‑134 (MQ=255)                                                                                                         gCCAACGCAGACCTGGCGGCC‑GGCC‑GCCTGGACGGCGTGATTGCCGACAAATTCCCGCTGCACGAGTGGATGAACAAGAACCGCGGGGATTGCTGGAAGATCCTTGGCGACGTGGCGGACCCCAAGGCCGATgcc                           >  5:146400/1‑135 (MQ=255)                                                                                                           cAACGCAGACCTGGCGGCC‑GGCC‑GCCTGGCCCGCGTGATTGCCGCCAAAATACCGCTGCACGAGTGGATGAACAAAAACCGCGGGGATTGCTGCAAGATCCTTGGCGACGTGGCGGCCCCCCAGGCCGCTgccgc                         >  4:22880/1‑135 (MQ=255)                                                                                                           cAACGCAGACCTGGCGGCC‑GGCC‑GCCTGGACGGCGGGATTGCCGACAAATTACCGCTGCACGAGTGGATGCACAATCACGGCGGGGAGTGCTGCAAGATACTTGGCGCCGTGGCGGACCCCACGGCCGATgccgc                         >  1:245757/1‑135 (MQ=255)                                                                                                           cAACGCAGACCTGGCGGCC‑GGCC‑GCCGGGACGGCGGGTTTGGCGACAAATTGCCGCTGCACGAGTGGAAGTACAACAACGGCGGGGATTGCTGCCAGATCCGTGGCGCCGTGGGGGGCCCCAACGCCCAGgccc                          >  5:91099/1‑133 (MQ=255)                                                                                                                cAGACCTGGCGGCC‑GGCC‑GCCCGGGCGGCGGGTTTGCCGCCAAATTACCCCGGCCCGAGTGGATGGACAAGAAAGGCGGGGGTTGCGGGCATATCCTTGGCGACCTGGCGGCCACCCACGCCGCTTCCGCCCTCg                    >  2:5917/1‑135 (MQ=255)                                                                                                                        gcggcg‑GGCC‑GCCGGGACGGCGTGATTGCCGACAAATTCCCGCTGCACGAGTGGATGAACAAGAACGGCGGGGATTGCTGCAAGATCCTTGGCGACGTGGCGGACACCAAGGCCGATGCCGCCATCGCTGTgcgc            <  3:7324/135‑1 (MQ=255)                                                                                                                        gcggcC‑GGCC‑GCCTGGACGGCGTGATTGCCGACAAATTCCCGCTGCACGAGGGGATGAACAAGAACGGCGGGGATTGCTGCAAGATCCTTGGCGACGTGGCGGACACCAAGGCCGATGCCGCCATCGCTGTgcgc            <  4:229735/135‑1 (MQ=255)                                                                                                                           gcc‑ggccgGCCTGTAGGGGGTGATTGCCCAAAAATTCCCGCTGCACGTGTGGATGAAAAAGCACGGCGGGGATTGCTGCAAGATCCTTGGCGACGTGGCGGACACCAAGGCCGATGCCGCCATCGCTGTGCGCaa          <  5:88342/135‑1 (MQ=255)                                                                                                                           gcc‑ggcc‑gCCTGGACGGCGGGATTGCCGACAAATTCCCGCTGCACGAGTGGATGAACAAGAACGGCGGGGATTGCTGCAAGCTCCTTGGCGACGTGGCGGCCACCAAGGCCGATGCCGCCCTCGCTGTGCGCaac         >  7:58999/1‑134 (MQ=255)                                                                                                                                    gccTGGACGGCGTGATTGCCGACAAATTCCCGCTGCACGAGTGGATGAACAAGAACGGCGGGGATTGCTGCAAGATCCTTGGCGACGTGGCGGACACCAAGGCCGATGCCGCCATCGCTGTGCGCAAGGacgacg  >  8:75899/1‑135 (MQ=255)                                                                                                                                       |                                                                                                                                    GCTACACCGTCGGTGCGCAGTCTTCCTCTACCCAGGCCATCTACGCCGAAGACGTGTACGGCAAGGCCGGTGCCGACGTGAAACTGTACCCGACCATGGACGAAGCCAACGCAGACCTGGCGGCC‑GGCC‑GCCTGGACGGCGTGATTGCCGACAAATTCCCGCTGCACGAGTGGATGAACAAGAACGGCGGGGATTGCTGCAAGATCCTTGGCGACGTGGCGGACACCAAGGCCGATGCCGCCATCGCTGTGCGCAAGGACGACG  >  KT2440/4077032‑4077295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑