Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 4,505,789 G→T 8.1% P94Q (CCG→CAG)  lolA ← Outer‑membrane lipoprotein carrier protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,505,7890GT 8.1% 120.1 / 2.0 45P94Q (CCG→CAG) lolAOuter‑membrane lipoprotein carrier protein
Reads supporting (aligned to +/- strand):  ref base G (21/20);  new base T (2/2);  total (23/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CATTACTTCGCCCTGCTCCTTGGACGCAATATCAAAGCTCTGGCTGATCTTCGACACGTCACCGGACAGCAACAAGGCCGGGGTCTGGTTCAGGCGCACGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCGGGTCCCACAGGGTGACGTTCTTGCCGTCCGACACCACCACCTGCTCCTGCGGTGCATCGGTGTGCCAGTAGAACAGGCCCGGGCGCTTGACCGTCATCTTGCCCGAGGTTTCCTGCAGGCTGGTGCCAT  >  KT2440/4505656‑4505918
                                                                                                                                     |                                                                                                                                 
cATTACTTCGCCCTGCTCATTGGAAGCAATATCAAAGCTCTGGCTGATCTTCGACACGTCACCGGACAGCAACAAGGCCGGTGTCTGGTTCAGGCGCACGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCgg                                                                                                                                  <  5:3428/135‑1 (MQ=255)
   tACTTCGCCCTGCTCCTTGGACGCAATATCAAAGCTCTGGCTGATCTTCGACACGTCACCGGACAGCAACAAGGCCGGGGTCTGGTTCAGGCGCACGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCGGGTc                                                                                                                               <  3:144545/135‑1 (MQ=255)
   tACTTCGCCCTGCTCCTTGGACGCAATATCAAAGCTCTGGCTGATCTTCGACACGTCACCGGACAGCAACAAGGCCGGGGTCTGGGTCAGGCGCACGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCGGGTc                                                                                                                               <  5:294768/135‑1 (MQ=255)
   tACTGCGCCCTGCTCCTTGGACGCAATATCAAAGCTCTGGCTGATCTTCGACACGTCACCGGACAGCAACAAGGCCGGGGTCTGGTTCAGGCGCACGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCGGGTc                                                                                                                               <  7:177681/135‑1 (MQ=255)
    aCTTCGCCCTGCTCCTTGGACGCAATATCAAAGCTCTGGCTGATCGTCGACACGTCACCGGACAGCAACAAGGCCGGGGTCTGGTTCAGGCGCACGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCGGGTcc                                                                                                                              >  3:28723/1‑135 (MQ=255)
           ccTGCTCCTTGGACGCAATATCAAAGCTCTGGCTGATCTTCGACACGTCACCGGACAGCAACAAGGCCGGGGTCTGGTTCAGGCGCACGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCGGGTc                                                                                                                               <  8:186622/127‑1 (MQ=255)
           ccTGCTCCTTGGACGCAATATCAAAGCTCTGGCTGATCTTCGACACGTCACCGGACAGCAACAAGGCCGGGGTCTGGTTCAGGCGCACGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCGGGTc                                                                                                                               >  7:186622/1‑127 (MQ=255)
           ccTGCTCCTTGGACGCAATATCAAAGCTCTGGCTGATCTTCGACACGTCACCGGACAGCAACAAGGCCGGGGTCTGGTTCAGGCGCACGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCGGGTCCCACAggg                                                                                                                       >  3:224998/1‑135 (MQ=255)
           ccTGCTCCTTGGACGCAATATCAAAGCTCTGGCTGATCTTCGACACGTCACCGGACAGCAACAAGGCCGGGGTCTCGTTCAGGCGCACGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCGGGTCCCACAggg                                                                                                                       >  6:16242/1‑135 (MQ=255)
             tGCTCCTTGGACGCAATATCAAAGCTCTGGCTGATCTTCGACACGTCACCGGACAGCAACAAGGCCGGGGTCTGGTTCAGGCGCACGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCGGGTCCCACAGGGTg                                                                                                                     >  2:200194/1‑135 (MQ=255)
               cTCCTTGGACGCAATATCAAAGCTCTGGCTGATCTTCGACACGTCACCGGACAGCAACAAGGCCGGGGTCTGGTTCAGGCGCACGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCGGGTc                                                                                                                               >  3:95881/1‑123 (MQ=255)
               cTCCTTGGACGCAATATCAAAGCTCTGGCTGATCTTCGACACGTCACCGGACAGCAACAAGGCCGGGGTCTGGTTCAGGCGCACGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCGGGTc                                                                                                                               <  4:95881/123‑1 (MQ=255)
                       aCGCAATATCAAAGCTCTGGCTGATCTTCGACACGTCACCGGACAGCAACAAGGCCGGGGTCTGGTTCAGGCGCACGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCGGGTCCCACAggg                                                                                                                       <  3:130677/123‑1 (MQ=255)
                       aCGCAATATCAAAGCTCTGGCTGATCTTCGACACGTCACCGGACAGCAACAAGGCCGGGGTCTGGTTCAGGCGCACGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCGGGTCCCACAggg                                                                                                                       >  4:130677/1‑123 (MQ=255)
                            atatCAAAGCTCTGGCTGATCTTCGCCACGTCACCGGACAGCAACAAGGCCGGGGACTGGTTCAGGCGCACGTCGAGCTGCTTGATGGTCGCCTGTTCCAGGTCCGGGTCCCACAGGGTGACGTTCTTGCCGTc                                                                                                       >  3:23942/1‑134 (MQ=255)
                             tatCAAAGCTCTGGCTGATCTTCGACACGTCACCGGACAGCAACAAGGCCGGGGTCTGGTTCAGGAGCACGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCGGGTCCCACAGGGTGACGTTCTTGCCGTCCg                                                                                                     <  8:135045/135‑1 (MQ=255)
                              atCAATGCTCTGGCTGATCTTCGTCACGTCACCGGACAGCAAGAAGGCCGGGGTCTGGTTCAGGCGCAGGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCGGGTCCCACAGGGTGACGTTCTTGCCGTCCGa                                                                                                    <  8:75672/135‑1 (MQ=255)
                               tCAAAGCTCTGGCTGATCTTCGACACGTCACCGGACAGCAACAAGGCCGGGGTCTGGTTCAGGCGCACGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCGGGTCCCACAGGGTGACGTTCTTGCCGTCCGac                                                                                                   >  8:193101/1‑135 (MQ=255)
                                               tcctCGACACGTCACCGGACAGCAACAAGGCCGGGGGCTGGTTCAGGCGCACGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCGGGTCCCACAGGGTGACGTTCTTGCCGTCCGACACCACCACCTGCTc                                                                                     <  3:82922/130‑1 (MQ=255)
                                               tCTTCGACACGTCACCGGACAGCAACAAGGCCGGGGTCTGGTTCAGGCGCACGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCGGGTCCCACAGGGTGACGTTCTTGCCGTCCGACACCACCACCTGCTc                                                                                     <  1:21215/133‑1 (MQ=255)
                                                    gACACGTCACCGGACAGCAACCAGGCCGGGGTCTGGTTCAGGCGCACGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCGGGTCCCACAGGGTGACGTTCTTGCCGTCCGACACCACCACCTGCTCCTGCg                                                                                <  4:23942/133‑1 (MQ=255)
                                                           cACCGGACAGCAACAAGGCCGGGGTCTGGTTCAGGCGCACGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCGGGTCCCACAGGGTGACGTTCTTGCCGTCCGACACCACCACCTGCTCCTGCGGTGCATCgg                                                                       <  2:76953/135‑1 (MQ=255)
                                                           cACCGGACAGCAACAAGGCCGGGGTCTGGTTCAGGCGCACGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCGGGTCCCACAGGGTGACGTTCTTGCCGTCCGACACCACCACCTGCTCCTGCGGTGCATCgg                                                                       <  5:273357/135‑1 (MQ=255)
                                                            aCCGGACAGCAACAAGGCCGGGGTCTGGTTCAGGCGCACGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCGGGTCCCACAGGGTGACGTTCTTGCCGTCCGACACCACCACCTGCTCCTGCGGTGCATCGgt                                                                      >  2:111952/1‑135 (MQ=255)
                                                                     caacaaGGCCGGGGTCTGGTTCAGGCGCACGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCGGGTCCCACAGGGTGACGTTCTTGCCGTCCGACACCACCACCTGCTCCTGCGGTGCATCGGTGTGCCAGTa                                                             <  5:87255/135‑1 (MQ=255)
                                                                        caaGGCCGGGGTCTGGTTCAGGCGCACGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCTGGTCCCACAGGGTGACGTTCTTGCCGTCCGACACCACCACCTGCTCCTGCGGTGCATCGGTGTGCCAGTAGaa                                                          >  1:221181/1‑135 (MQ=255)
                                                                        caaGGCCGGGGTCTGGTTCAGGCGCACGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCTGGTCCCACAGGGTGACGTTCTTGCCGTCCGACACCACCACCTGCTCCTGCGGTGCATCGGTGTGCCAGTAGa                                                           >  1:224978/1‑134 (MQ=255)
                                                                        caaGGCCGGGGTCTGGTTCAGGCGCACGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCGGGTCCCACAGGGTGACGTTCTTGCCGTCCGACACCACCACCTGCTCCTGCGGTGCATCGGTGTGCCAGTAGa                                                           >  5:151245/1‑134 (MQ=255)
                                                                        caaGGCCGGGGTCTGGTTCAGGCGCACGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCGGGTCCCACAGGGTGACGTTCTTGCCGTCCGACACCACCACCTGCTCCTGCGGTGCATCGGTGTGCCAGTAGa                                                           >  2:152258/1‑134 (MQ=255)
                                                                                  gTCTGGTTCAGGCGCACGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCGGGTCCCACAGGGTGACGTTCTTGCCGTCCGACACCACCACCTGCTCCTGCGGTGCATCGGTGTGCCAGTAGAACAGGCCCggg                                                >  4:204404/1‑135 (MQ=255)
                                                                                    cTGGTTCAGGCGCACGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCGGGTCCCACAGGGTGACGTTCTTGCCGTCCGACACCACCACCTGCTCCTGCGGTGCATCGGTGTGCCAGTAGAACAGGCCCGGgcg                                              <  4:224998/135‑1 (MQ=255)
                                                                                               gcAAGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCGGGTCCCACAGGGTGACGTTCTTGCCGTCCGACACCACAACCTGCTCCTGCGGTGCATCGCTGTGCCAGTAGAACAGGCCCGGGCGCTTGACCCTCa                                   >  7:183182/1‑135 (MQ=255)
                                                                                                cACGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCGGGTCCCACAGGGTGACGTTCTTGCCGTCCGACACCACCACCTGCTCCTGCGGTGCATAGGTGTGCCAGTAGAACAGGCCCGGGCGCGTGACCGGCaa                                  >  7:144367/1‑134 (MQ=255)
                                                                                                    tCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCGGGTCCCACAGGGTGACGTTCTTGCCGTCCGACACCACCACCTGCTCCTGCGGTGCATCGGTGTGCCAGTAGAACAGGCCCGGGCGCTTGACCGTCATCTTg                              <  3:297838/135‑1 (MQ=255)
                                                                                                         cttcttGATGGTGGCCTGTTCCAGGTCCGGGTCCCACAGGGTGACGTTCTTGCCGTCCGACACCACCACCTGCTCCTGCGGTGCATCGGTGTGCCAGTAGAACAGGCCCGGGCGCTTGACCGTCATCTTGCCCGa                         <  1:200194/135‑1 (MQ=255)
                                                                                                          ttcttGATGGTGGCCTGTTCCAGGTCCTGGTCCCACAGGGTGACGTTCTTGCCGTCCGACACCACCACCTGCTCCTGCGGTGCATCGGTGTGCCAGTAGAACAGGCCCGGGCGCTTGACCGTCATCTTGCCCGAg                        <  2:221181/135‑1 (MQ=255)
                                                                                                          ttcttGATGGTGGCCTGTTCCAGGTCCTGGTCCCACAGGGTGACGTTCTTGCCGTCCGACACCACCACCTGCTCCTGCGGTGCATCGGTGTGCCAGTAGAACAGGCCCGGGCGCTTGACCGTCATCTTGCCCGAg                        <  2:224978/135‑1 (MQ=255)
                                                                                                          ttcttGATGGTGGCCTGTTCCAGGTCCGGGTCCCACAGGGTGACGTTCTTGCCGTCCGACACCACCACCTGCTCCTGCGGTGCATCGGTGTGCCAGTAGAACAGGCCCGGGCGCTTGACCGTCATCTTGCCCGAg                        >  7:204108/1‑135 (MQ=255)
                                                                                                          ttcttGATGGTGGCCTGTTCCAGGTCCGGGTCCCACAGGGTGACGTTCTTGCCGTCCGACACCACCACCTGCTCCTGCGGTGCATCGGTGTGCCAGTAGAACAGGCCCGGGCGCTTGACCGTCATCTTGCCCGAg                        <  4:31373/135‑1 (MQ=255)
                                                                                                                aTGGTGGCCTGTTCCAGGTCCGGGTCCCACAGGGTGACGTTCTTGCCGTCCGACACCACCACCTGCTCCTGCGGTGCATCGGTGTGCCAGTAGAACAGGCCCGGGCGCTTGACCGTCCTCTTGCCCGAGGTTTcc                  >  8:66556/1‑135 (MQ=255)
                                                                                                                aTGGTGGCCTGTTCCAGGTCCGGGTCCCACACGGTGACGTTCTTGCAGTCCGAAACCACCACCTGCTCCTGCGGTGCATCGGTGTGACAGTAGAACAGGCCCGGGCCCTTGTACGTAATCATGCCCGAgcgatcc                  >  7:238508/1‑129 (MQ=255)
                                                                                                                    tggCCTGTTCCAGGTCCGGGTCCCACAGGGTGACGTTCTTGCCGTCCGACACCACCACCTGCTCCTGCGGTGCATCGGTGTGCCAGTAGAACAGGCCCGGGCGCTTGACCGTCATCTTGCCCGAGGTTTCCTGCa              <  7:193101/135‑1 (MQ=255)
                                                                                                                       ccTGTTCCAGGTCCGGGTCCCACAGGGTGACGTTCTTGCCGTCCGACACCACCACCTGCTCCTGCGGTGCATCgg                                                                       >  6:237983/1‑75 (MQ=255)
                                                                                                                       ccTGTTCCAGGTCCGGGTCCCACAGGGTGACGTTCTTGCCGTCCGACACCACCACCTGCTCCTGCGGTGCATCgg                                                                       <  5:237983/75‑1 (MQ=255)
                                                                                                                              cAGGTCCGGGTCCCACAGGGTGACGTTCTTGCCGTCCGACACCACCACCTGCTCCTGCGGTGCATCGGTGTGCCAGTAGAACAGGCCCGGGCGCTTGACCGTCATCTTGCCCGAGGTTTCCTGCAGGCTGGTGcc    >  2:36400/1‑135 (MQ=255)
                                                                                                                                ggTCCGGGTCCCACAGGGTGACGTTCTTGCCGTCCGACACCACCACCTGCTCCTGCGGTGCATCGGTGTGCCAGTAGAACAGGCCCGGGCGCTTGCCCGTCATCTTGCCCGCGGTTTCCTGCAGGCTGGTGCCAt  >  1:98919/1‑135 (MQ=255)
                                                                                                                                     |                                                                                                                                 
CATTACTTCGCCCTGCTCCTTGGACGCAATATCAAAGCTCTGGCTGATCTTCGACACGTCACCGGACAGCAACAAGGCCGGGGTCTGGTTCAGGCGCACGTCGAGCTTCTTGATGGTGGCCTGTTCCAGGTCCGGGTCCCACAGGGTGACGTTCTTGCCGTCCGACACCACCACCTGCTCCTGCGGTGCATCGGTGTGCCAGTAGAACAGGCCCGGGCGCTTGACCGTCATCTTGCCCGAGGTTTCCTGCAGGCTGGTGCCAT  >  KT2440/4505656‑4505918

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑