| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 4,666,745 | T→G | 34.6% | intergenic (‑90/‑131) | lasR ← / → btuB_4 | Transcriptional activator protein LasR/Vitamin B12 transporter BtuB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 4,666,745 | 0 | T | G | 34.6% | 27.7 / 12.7 | 33 | intergenic (‑90/‑131) | lasR/btuB_4 | Transcriptional activator protein LasR/Vitamin B12 transporter BtuB |
| Reads supporting (aligned to +/- strand): ref base T (4/17); new base G (10/2); total (14/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.44e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGT‑GGTTTGTAAAGAACTTTCGTACAAGACCGTATGACGAAGGATGCGCACGATTTGCTGACTTTCTGCGTGCTCCAAGCCGCATTTCGCTCGTCTCAACGCCGTTGTCCATTCGCCTTTCGCCTGGAGTCGTACAATGC > KT2440/4666621‑4666879 | aGGGGTGGCTGCGAGTGGCGAGCGTTGTCAGTCATGTTTGAACTCCCCGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGT‑GGTTTGTAAAGaa < 5:84861/135‑1 (MQ=255) gcGTGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGT‑GGTTTGTAAAGAACTTTGGTACa < 2:87948/135‑1 (MQ=255) gcGAGTGTCTAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGT‑GGTTTGTAAAGAACTTTCGTACa < 7:287006/135‑1 (MQ=255) gcGAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCCCCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGT‑GGTTTGTAAAGAACTGTCGtc > 8:186275/1‑132 (MQ=255) gcGAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGT‑GGTTTGTAAAGAACTTTCGTACa < 4:292674/135‑1 (MQ=255) gtCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGT‑GGGTTGTAAAGAACTTTCGTACAAGAcc > 3:296142/1‑134 (MQ=255) tcagtcaTGTTTGAAATCCAAGGTTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGT‑GGGTTGTTAAGAACTTTCGGGCCAGACCGGATGACGGAgg > 7:162994/1‑135 (MQ=255) agtcaTGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGT‑GGGTTGTTAAGAACTTTAGTTCAAGACCGTATTACCAATGAt > 7:103334/1‑135 (MQ=255) gAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGT‑GGTTTGTAAAGAACTTTCGTACAAGACCGTATGACGAAGGATGCGCACGAt < 7:132111/134‑1 (MQ=255) gggTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGT‑GGTTTGTAAAGAACGTTGGTACAAGACCGGATGACGAAGGATGCGCCCGATTTTGTGAcgt > 6:261227/1‑133 (MQ=255) tGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGT‑GGTTTGTAAAGAACTTTCGTACAAGACCGTATGACGAAGGATGCGCACGATTTGCTGACTTTCt < 5:121520/135‑1 (MQ=255) gAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGTGGGGTTTTAAAAAACTTTCGTACAAACCCGTATTCCGAAGGATGCGCCCGAATTGCTGACTTACTGGGt > 1:133095/1‑135 (MQ=255) aTTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGTCTCCACCGCCGT‑GGTTTGTAAAGAACTTTCGTACAAGACCGTATGACGAAGGATGCGCACGATTTGCTGACTTTCTGCGTGCTCCAAGcc < 6:66253/135‑1 (MQ=255) aTTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGT‑GGTTTGTAAAGAACTTTCGTACAAGACCGTATGACGAAGGATGCGCACGCTTTGCTGACTTTCTGCGTGCTCCAAGcc > 7:271058/1‑135 (MQ=255) ccTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGT‑TGTTTGTAAAGAACTTTCGTACAAGACCGTATGACGAAGGATGCGCACGATTTGCTGTCTTTCTGCGTGCTCCAAGCCGCATTTc < 2:250779/135‑1 (MQ=255) aCCTGCAGTCCCCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGT‑GGGTTGTAAAGAACTTTCGTACCAGCCCGTTTGCCGCAGGCTGCGCACGCGTGGCTGCCGTTCTGCGGGGTCCCACCCGCATTTCGCt > 4:296175/1‑135 (MQ=255) aCCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGT‑GGGTTGTTAAGAACGTTAGGTCAAGCCCGGAAGACGCACGCTTGGCCCCCGTTTGTGGCTTTCTTCGTGCTCCAAGCCGCATTTCggt > 2:114533/1‑133 (MQ=255) cTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGT‑GGGTTGTAAAGAACTTTCGTACAAGACCGTATGACGAAGGATGCGCACGATTTGCTGACTTTCTGCGTGCTCCAAGCCGCATTTCGCTCg < 3:9041/135‑1 (MQ=255) aGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGT‑GGTTTGTAAAGAACTTTCGTACAAGACCGTATGACGAAGGATGCGCACGATTTGCTGACTTTCTGCGTGCTCCAAGCCGCATTTCGCTCGtct < 7:69736/134‑1 (MQ=255) aGTCAACTTACGACGCACAGGCCACCTGGCTCCAGCGCCGT‑GGTTTGTAAAGAACTTTCGTTCAAGACCGTATGACGAAGGATGCGCACGATTTGCTGACTTTCTGCGTGCTCCAAGCCGCATTTCGCTCGtct < 2:271798/134‑1 (MQ=255) cACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGT‑GGTTTGTAAAGAACTTTCGTACAAGACCGTATGACGAAGGATGCGCACGATTTGCTGACTTTCTGCGTGCTCCAAGCCGCATTTCGCTCGTCTCAAc < 8:4289/135‑1 (MQ=255) cTTACGAAGCACAGGCCACCTGCCTCCACCGCCGT‑GGTTTGTAAAGAACTTTCGTACAAGACCGTATGACGAAGGATGCGCACGATTTGCTGACTTTCTGCGTGCTCCAAGCCGCATTTCGCTCGTCTCAACGcc < 6:162932/135‑1 (MQ=255) ttACGAAGCACAGGCCACCTGCCTCCACCGCCGT‑GGGTTGTAAAGAACTTTCGTTCAATACCGTATGACGAAGGCTGCGCCCGATTTGCTGACTTTATGCGTGCTCCAAGCCGCATTTAGCTCGTCTCAACGCCg > 2:7326/1‑135 (MQ=255) ggCCACCTGCCTCCACCGCCGT‑GGGTTGTTAAGAACATTCGTACAAAACCGTTTGACGAAGGCTGCGCACCATTTTCTGTCTTTATTCGTGCTCCAAGCCGCATTTGGCGCGTCGCAACCCCGTTGTGCCttcgc > 4:101414/1‑135 (MQ=255) aCCTGCCTCCACCGCCGT‑GGGTTGTTATGAACTTTCGTAAAAGACCGTATGACGAAGGATGCGCACGAGTTGGTGGCTTTCTGGGTGCTCCAAGCCGCCTTTAGCTCGTCTCAACGCCCGTGTCCAttcgcct > 6:88439/1‑133 (MQ=255) ccTGCCTCCACCGCCGT‑GGGTTGTAAAGAACTTTCGTACAAGACCGTATGACGAAGGATGCGCCCGATTTGCTGAGTTGCTGCGTGCTCCAAGCCGCATTTCGCTCGTCTCAACGCCGTTGTCCAttcgcctttc < 7:144164/135‑1 (MQ=255) tGCCTCCACCGCCGT‑GGTTTGTAAAGAACTTTCGTACAAGACCGTATGACGAAGGATGCGCACGATTTGCTGACTTTCTGCGTGCTCCAAGCCGCATTTCGCTCGTCTCAACGCCGTTGTCCAttcgcctttcgc < 3:47789/135‑1 (MQ=255) ccTCCACCGCCGT‑GGTTTGTTAATAAATTTCGTAAAAGACCGGATGCAGAAGGATGCGGACGATTTGCTGAATTTCTGCGTGCTCCAATCCGCATTTAGCGAGTCTCAACGCCCGTGTCCAttagcctttcgcct > 6:77907/1‑135 (MQ=255) ccTCCACCGCCGT‑GGGTTGTTAAGAACATTCGTAAAAGACCGTATGACGAAGGATGCGGCCGATTTTCGGGCTTTCTTGGTGCTGCCAGCCGCATTTAGCTCGGCTCAACGCCGGTGTCCAttcgccgttagccg > 8:120095/1‑134 (MQ=255) cTCCACCGCCGT‑GGTTTGTAACGAACTTTCGTACAAGACCGTATGACGAAGGATGCGCACGATTTGCTGACTTTCTGCGTGCTCCAAGCCGCATTTCGCTCGTCTCAACGCCGTTGTCCATTCGCCTTTCGCCTg < 2:290879/135‑1 (MQ=255) cTCCACCGCCGT‑GGTTTGTAAAGAACTTTCGTACAAGACCGTATGACGAAGGATGCGCACGATTTGCTGACTTTCTGCGTGCTCCAAGCCGCATTTCGCTCGTCTCAACGCCGTTGTCCATTCGCCTTTCGCCTg < 3:222913/135‑1 (MQ=255) cTCCACCGCCGT‑GGTTTGTAAAGAACTTTCGTACAAGACCGTATGACGAAGGATGCGCACGATTTGCTGACTTTCTGCGTGCTCCAAGCCGCATTTCGCTCGTCTCAACGCCGTTGTCCATTCGCCTTTCGCCTg < 2:210080/135‑1 (MQ=255) gccgT‑GGTTTGTAAAGAACTTTCGTACAAGACCGTATGACGAAGGATGCGCACGATTTGCTGACTTTCTGCGTGCTCCAAGCCGCATTTCGCTCGTCTCAACGCCGTTGTCCATTCGCCTTTCGCCTGGAGTCGt < 8:103334/135‑1 (MQ=255) tttGTAAAGAACTTTCGTACAAGACCGTATGACGAAGGATGCGCACGATTTGCTGACTTTCTGCGTGCTCCAAGCCGCATTTCGCTCGTCTCAACGCCGTTGTCCATTCGCCTTTCGCCTGGAGTCGTACAATGc < 8:271058/135‑1 (MQ=255) tttGTAAACAACATTAGTACAAGACCCTATTACGAAGGATGCGCACGATTTGCGGACTTTGTGCGTGCTCCAAGCCGCCTTTCGCTCGTCTCACCGCCGTTGTGCATTCGCCTTTCGCCTGGAGGAGTACCATGc > 8:264228/1‑135 (MQ=255) | AGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGT‑GGTTTGTAAAGAACTTTCGTACAAGACCGTATGACGAAGGATGCGCACGATTTGCTGACTTTCTGCGTGCTCCAAGCCGCATTTCGCTCGTCTCAACGCCGTTGTCCATTCGCCTTTCGCCTGGAGTCGTACAATGC > KT2440/4666621‑4666879 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |