Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 5,818,139 C→A 8.9% L19M (CTG→ATG)  CJ019_05447 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,818,1390CA 8.9% 126.7 / 2.5 42L19M (CTG→ATG) CJ019_05447hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (21/17);  new base A (2/2);  total (23/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

AGCCACGCCGAGGGGCCAGTTGTTCCTCAATGACCTGCTGCAGTATTTCTTGACCTGAGGATGACCCATGGATCTGGTACTTGATCTGCTCGCGACGGTTTCCCGCTGGAGCCGCAGCAACCTGTCGGAGATTTCACTGGCCTTGGTAGGCTGCCTGCTGGTGCTGTTCGGCACCGATGTCAAAGCCTGGGCCGAGCAGCGCCTGGGCGGCCTCGCGGGCGCCCTGCGCGTGCCGTTCATGGCGCTGATGGTCATG  >  KT2440/5818018‑5818273
                                                                                                                         |                                                                                                                                      
aGCCACGCCGAGGGGCCAGTTGTTCCTCAATGACCTGCTGCAGTATTTCTTGACCTGAGGATGACCCATGGATCTGGTACTTGATCTGCTCGCGACGGTTTCCCGCTGGAGCCGCAGCAACCTGTCGGAGATTTc                                                                                                                           <  6:151349/135‑1 (MQ=255)
  ccACGCCGTGGGGGAAGTTGTTCCTCAATTACCTGCTGAAGTATTTCTTGACCTGCGGATGACACAGGGTTCTGGTACTTGTGCTGCTCGCGACGGGTTCCCGCTGGAGCCGCAGCAACCTGTCGGAGATTTCAc                                                                                                                         <  5:21863/135‑1 (MQ=255)
  ccACGCCGAGGGGCCAGTTGTTCCTCAATGACCTGCTGCAGTATTTCTTGACCTGAGGATGACCCATGGATCTGGTACTTGATCTGCTCGCGACGGTTTCCCGCTGGAGCCGCAGCAACCTGTCGGAGATTTCAc                                                                                                                         <  7:42804/135‑1 (MQ=255)
         gAGGGGCCAGTTGTTCCTCAATGACCTGCTGCAGTATTTCTTGACCTGAGGATGACCCATGGATCTGGTACTTGATCTGCTCGCGACGGTTTCCCGCTGGAGCCGCAGCAACCTGTCGGAGATTTCACTGGCCtt                                                                                                                  >  3:286961/1‑135 (MQ=255)
           ggggCCAGTTGTTCCTCAATGACCTGCTGCAGTATTTCTTGACCTGAGGATGACCCATGGATCTGGTACTTGATCTGCTCGCGACGGTTTCCCGCTGGAGCCGCAGCAACCTGTCGGAGATTTCACTGGCCTTg                                                                                                                 >  3:47788/1‑134 (MQ=255)
               cctgttgttCCTCAATGACCTGCTGCAGTATTTCTTGACCTGAGGATGACCCATGGATCTGGTACTTGATCTGCTCGCGAGGGTTTCCCGCTGGAGCCGCAGCAACCTGTCGGAGATTTCACTGGCCTTGGTAgg                                                                                                            <  8:152020/132‑1 (MQ=255)
               ccAGTTGTTCCTCAATGCCCTGCTGCAGTATTTCTTGACCTGAGGATGACCCATGGATCTGGTACTTGATCTGCTCGCGACGGTTTCCCGCTGGAGCCGCAGCAACCTGTCGGAGATGTCACTGGCCTTGGTAgg                                                                                                            >  5:167766/1‑135 (MQ=255)
               ccAGTTGTTCCTCAATGACCTGCTGCAGTATTTCTTTACCTGAGGATGACCCATGGATCTGGTACTTGATCTGCTCGCGACGGTTTCCCGCTGGAGCCGCAGCAACCTGTCGGAGATTTCACTGGCCTTGGTAgg                                                                                                            <  8:113747/135‑1 (MQ=255)
               ccAGTTGTTCCTCAATGACCTGCTGCAGTATTTCTTGACCTGAGGATGACCCATGGATCTTGTACTTGATATGCTCGCGACGGTTTCCCGCTGGAGCCGCAGCAACCTGTCGGAGATTTCACTGGCCTTGGTAgg                                                                                                            >  6:183417/1‑135 (MQ=255)
               ccAGTTGTTCCTCAATGACCTGCTGCAGTATTTCTTGACCTGAGGATGACCCATGGATCTGGTACTTGATCTGCTCGCGACGGTTTCCCGCTGGAGCCGCAGCAACCTGTCGGAGATTTCACTGGCCTTGGTAgg                                                                                                            <  8:251543/135‑1 (MQ=255)
                         cTCAATGACCTGCTGCAGTATTTCTTGACCTGAGGATGACCCATGGATCTGGTACTTGATCTGCGCGCGACGGTTTCCCGCTGGAGCCGCAGCAACCTGTCGGAGATTTCACTGGCCTTGGTAGTCTGCctgctg                                                                                                  <  6:147277/135‑1 (MQ=255)
                          tCAATGACCTGCTGAAGTTTTTCTTTACCTGAGGATGACCAATTGTTCTGGTTCTTGATCTGCTCGCGACGGTTTCCAGCTGGAGCCGGATCAACCTGTCGGAGATTTCACTGGCCTTGTTAGGCTGCctgctt                                                                                                  <  2:73146/134‑2 (MQ=255)
                           cAATGACCTGCTGCAGTATTTCTTGACCTGAGGATGACCCATGGATCTGGTACTTGATCTGCTCGCGACGGTTTCCCGCTGGAGCCGCAGCAACCTGTCGGAGATTTCACTGGCCTTGGTAGGCTGCCTGCTGGt                                                                                                >  1:211631/1‑135 (MQ=255)
                           cAATGACCTGCTGCAGTATTTCTTGACCTGAGGATGACCCATGGATCTGGTACTTGATCTGCTCGCGACGGTTTCCCGCTGGAGCCGCAGCAACCTGTCGGAGATTTCACTGGCCTTGGTAGGCTGCCTGCTGGt                                                                                                >  1:215564/1‑135 (MQ=255)
                                 ccTGCTGCAGTATTTCTTGACCTGAGGATGACCCATGGATCTGGTACTTGATCTGCTCGCGACGGTTTCCCGCTGGAGCCGCAGCAACCTGTCGGAGATTTCACTGGCCTTGGTAGGCTGCCTGCTGGTGCTGtt                                                                                          >  5:287146/1‑135 (MQ=255)
                                      tgcAGTATTTCTTGACCTGAGGATGACCCATGGTTCTGGTACTTGATCTGCTCGCGACGGTTTCCCGCTGGAGCCGCAGCAACCTGTCGGAGATTTCACTGGCCTTGGTAGGCTGCCTGCTGGTGCTGTTCGGCa                                                                                     <  8:87123/135‑1 (MQ=255)
                                      tgcAGTATTTCTTGACCTGAGGATGACCCATGGATCTGGTACTTGATCTGCTCGCGACGGTTTCCCGCTGGAGCCGCAGCAACCTGTCGGAGATTTCACTGGCCTTGGTAGGCTGCCTGCTGGTGCTGTTCGGCa                                                                                     >  3:247787/1‑135 (MQ=255)
                                            aTTTCTTGACCTGAGGATGACCCATGGATCTGGTACTTGATCTGCTCGCGACGGTTTCCCGCTGGAGCCGCAGCAACCTGTCGGAGATTTCACTGGCCTTGGTAGGCTGCCTGCTGGTGCTGTTCGGCACCGAt                                                                                <  4:157592/134‑1 (MQ=255)
                                             tttCTTGACCTGAGGATGACCCATGGATCTGGTACTTGATCTGCTCGCGACGGTTTCCCGCTGGAGCCGCAGCAACCTGTCGGAGATTTCACTGGCCTTGGTAGGCTGCCTGCTGGTGCTGTTCGGCACCGATg                                                                               >  3:18202/1‑134 (MQ=255)
                                                   gACCTGAGGATGACCCATGGATCTGGTACTTGATCTGCTCGCGACGGTTTCCCGCTGGAGCCGCAGCAACCTGTc                                                                                                                                    <  7:132302/75‑1 (MQ=255)
                                                   gACCTGAGGATGACCCATGGATCTGGTACTTGATCTGCTCGCGACGGTTTCCCGCTGGAGCCGCAGCAACCTGTc                                                                                                                                    >  8:132302/1‑75 (MQ=255)
                                                      cTGAGGATGACCCATGGATCTGGTACTTGATCTGCTCGCGACGGTTTCCCGCTGGAGCTGCAGCAACCTGTCGGAGATTTCACTGGCCTTGGTAGGCTGCCTGCTGGTGCTGTTCGGCACCGATGTCAAAGCCTg                                                                     >  6:242475/1‑135 (MQ=255)
                                                          ggATGACCCATGGATCTGGTACTTGATCTGCTCGCGACGGTTTCCCGCTGGAGCCGCAGCAACCTGTCGGAGATTTCACTGGCCTTGGTAGGCTGCCTGCTGGTGCTGTTCGGGACCGATGTCAAAGCCTggg                                                                   <  8:158399/133‑1 (MQ=255)
                                                          ggATGACCCATGGATCTGGTACTTGATCTGCTCGCGACGGTTTCCCGCTGGAGCCGCAGCAACCTGTCGGAGATTTCACTGGCCTTGGTAGGCTGCCTGCTGGTGCTGTTCGGCACCGATGTCAAAGCCTGGGcc                                                                 <  4:18202/135‑1 (MQ=255)
                                                          ggATGACCCATGGAGCTGGTACTTGATCTGCGCGCGACGGTTTCCCGCTGGAGCCGCAGCAACCTGTCGGAGATTTCACTGGCCTTGGTAGGCTGCCTGCTGGTGCTGTTCGGCACCGATGTCAAAGCCTGGGcc                                                                 <  1:193629/135‑1 (MQ=255)
                                                            aTGACCCATGGATCTGGTACTTGATCTGCTCGCGACGGTTTCCCGCTGGAGCCGCAGCAACCTGTCGGAGATTTCACTGGCCTTGGTAGGCTGCCTGCTGGTGCTGTTCGGCACCGAGGTCAAAGCCTGGGCCGa                                                               >  7:262046/1‑135 (MQ=255)
                                                                  cATGGATCTGGTACTTGATCTGCTCGCGACGGTTTCCCGCTGGAGCCGCAGCAACCTGTCGGAGATTTCACTGGCCTTGGTAGGCTGCCTGCTGGTGCTGTTCGGCACCGATGTCAAAGCCTGGGCCGAGCAgcg                                                         >  8:160369/1‑135 (MQ=255)
                                                                     ggATCTGGTACTTGATCTGCTCGCGACGGTTTCCCGCTGGAGCCGCAGCAACCTGTCGGAGATTTCACTGGCCTTGGTAGGCTGCCTGCTGGTGCTGTTCGGCACCGATGTCAAAGCCTGGGCCGAGCAGCGCCt                                                      >  5:7062/1‑135 (MQ=255)
                                                                      gATCTGGTACTTGATCTGCTCGCGACGGTTTCCCGCTGGAGCCGCAGCAACATGTCGGAGATTTCACTGGCCTTGGTAGGCTGCCTGCTGGTGCTGTTCGGCACCGATGTCAAAGCCTGGGCCGAGCAGCGCCTg                                                     >  5:129079/1‑135 (MQ=255)
                                                                      gATCTGGTACTTGATCTGCTCGCGACGGTTTCCCGCTCGAGCCGCAGCAACATGTCGGAGATTTCACTGGCCTTGGTAGGCTGCCTGCTGGTGCTGTTCGGCACCGATGTCAAAGCCTGGGCCGAGCAGCGCCTg                                                     >  5:133992/1‑135 (MQ=255)
                                                                        tCTGGTACTTGATCTGCTCGCGACGGTTTCCCGCTGGAGCCGCAGCAACCTGTCGGAGATTTCACTGGCCTTGGTAGGCTGCCTTCTGGTGCTGTTCGGCACCGATGTCAAAGCCTGGGCCGAGCAGCGCCTggg                                                   >  7:118701/1‑135 (MQ=255)
                                                                             tACTTGATCTGCTCGCGACGGTTTCCCGCTGGAGCCGCAGCAACCTGTCGGAGATTTCACTGGCCTTGGTAGGCTGCCTGCTGGTGCTGTTCGGCACCGATGTCAAAGCCTGGGCCGAGCAGCGCCTGGGCGGcc                                              >  2:239118/1‑135 (MQ=255)
                                                                             tACTTGATCTGCTCGCGACGGTTTCCCGCTGGAGCCGCAGCAACCTGTCGGAGATTTCACTGGCCTTGGTAGGCTGCCTGCTGGTGCTGTTCGGCACCGATGTCAAAGCCTGGGCCGAGCAGCGCCTGGGCGGcc                                              >  1:197078/1‑135 (MQ=255)
                                                                              aCTTGATCTGCTCGCGACGGTTTCCCGCTGGAGCCGCAGCAACCTGTCGGAGATTTCACTGGCCTTGGTAGGCTGCCTGCTGGTGCTGTTCGGCACCGATGTCAAAGCCTGGGCCGAGCAGCGCCTGGGCGGCCt                                             >  1:135725/1‑135 (MQ=255)
                                                                              aCTTGATCTGCTCGCGACGGTTTCCCGCTGGAGCCGCAGCAACCTGTCGGAGATTTCACTGGCCTTGGTAGGCTGCCTGCTGGTGCTGTTCGGCACCGATGTCAAAGCCTGGGCCGAGCAGAGCCTGGGCCGCCt                                             >  1:145044/1‑135 (MQ=255)
                                                                                   aTCTGCTCGCGACGGTTTCCCGCTGGAGCCGCAGCAACATGTCGGAGATTTCACTGGCCTTGGTAGGCTGCCTGCTGGTGCTGTTCGGCACCGATGTCAAAGCCTGGGCCGAGCAGCGCCTGGGCGGCCTcgc                                          <  6:133992/133‑1 (MQ=255)
                                                                                   aTCTGCTCGCGACGGTTTCCCGCTGGAGCCGCAGCAACATGTCGGAGATTTCACTGGCCTTGGTAGGCTGCCTGCTGGTGCTGTTCGGCACCGATGTCAAAGCCTGGGCCGAGCAGCGCCTGGGCGGCCTcgc                                          <  6:129079/133‑1 (MQ=255)
                                                                                                   ttCCCGCTGGAGCCGCAGCAACCTGTCGGAGATTTCACTGGCCTTGGTAGGCTGCCTGCTGGTGCTGTTCGGCACCGATGTCAAAGCCTGGGCCGAGCAGCGCCTGGGCGGCCTCGCGTGCGCCCTGCGCGTGcc                        >  8:238685/1‑135 (MQ=255)
                                                                                                      ccGCTGGAGCCGCAGCAACCTGTCGGAGATTTCACTGGCCTTGGTAGGCTGCCTGCTGGTGCTGTTCGGCACCGATGTCAAAGCCTGGGCCGAGCAGCGCCTGGGCGGCCTCGCGGGCGCCCTGCGCGTGCCGt                      <  2:46809/134‑1 (MQ=255)
                                                                                                        gCTGGAGCCGCAGCAACCTGTCGGAGATTTCACTGGCCTTGGTAGGCTGCctgctg                                                                                                  <  7:167703/56‑1 (MQ=255)
                                                                                                        gCTGGAGCCGCAGCAACCTGTCGGAGATTTCACTGGCCTTGGTAGGCTGCctgctg                                                                                                  >  8:167703/1‑56 (MQ=255)
                                                                                                                   agcaACCTGTCGGAGATTTCACTGGCCTTGGTAGGCTGCCTGCTGGTGCTGTTCGGCACCGATGTCAAAGCCTGGGCCGAGCAGCGCCTGGGCGGCCTCGCGGGCGCCCGGCGCGTGCCGTTCATGGCGCTGATg        <  4:203868/135‑1 (MQ=255)
                                                                                                                        ccTGTCGGAGAGTTCACTGGCCTTGGTAGGCTGCCTGCTGGTGCTGTTCGGCACCGATGTCAAAGCCTGGGCCGAGCAGCGCCTGGGCGGGCTCGCGGGCGCGCTGCGCGTGCCGTTCATGGCGCTGATGGTGAt   <  7:100280/135‑1 (MQ=255)
                                                                                                                         cTGTCGGAGATTTCACTGGCCTTGGTAGGCTGCCTGCTGGTGCTGTTCGGCACCGATGTCAAAGCCTGGGCCGAGCAGCGCCTGGGCGGCCTCGCGGGCGCCCTGCGCGTGCCGTTCATGGCGCTGATGGTCATg  <  6:113162/135‑1 (MQ=255)
                                                                                                                         |                                                                                                                                      
AGCCACGCCGAGGGGCCAGTTGTTCCTCAATGACCTGCTGCAGTATTTCTTGACCTGAGGATGACCCATGGATCTGGTACTTGATCTGCTCGCGACGGTTTCCCGCTGGAGCCGCAGCAACCTGTCGGAGATTTCACTGGCCTTGGTAGGCTGCCTGCTGGTGCTGTTCGGCACCGATGTCAAAGCCTGGGCCGAGCAGCGCCTGGGCGGCCTCGCGGGCGCCCTGCGCGTGCCGTTCATGGCGCTGATGGTCATG  >  KT2440/5818018‑5818273

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑