Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 5,982,960 A→T 11.8% intergenic (+419/‑270) kefC_3 → / → CJ019_05604 Glutathione‑regulated potassium‑efflux systemprotein KefC/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,982,9600AT11.8% 90.3 / 5.3 34intergenic (+419/‑270)kefC_3/CJ019_05604Glutathione‑regulated potassium‑efflux systemprotein KefC/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (11/19);  new base T (2/2);  total (13/21)
Fisher's exact test for biased strand distribution p-value = 6.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01

CCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCC  >  KT2440/5982826‑5983076
                                                                                                                                      |                                                                                                                    
ccAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTcaca                                                                                                                      >  6:275370/1‑135 (MQ=255)
      aCCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTTGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATc                                                                                                                <  3:40610/135‑1 (MQ=255)
      aCCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATc                                                                                                                <  6:282826/135‑1 (MQ=255)
            aaGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACTCCAATCCCAAg                                                                                                           >  8:136925/1‑134 (MQ=255)
            aaGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACTCCAATCCCAAg                                                                                                           >  8:133383/1‑134 (MQ=255)
            aaGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAg                                                                                                           <  7:73461/134‑1 (MQ=255)
             aGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAg                                                                                                           <  1:290317/133‑1 (MQ=255)
             aGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAg                                                                                                           >  2:290317/1‑133 (MQ=255)
             aGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGa                                                                                                         <  1:196468/135‑1 (MQ=255)
             aGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGa                                                                                                         <  1:74241/135‑1 (MQ=255)
                   ccTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAACAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATc                                                                                                   <  8:132080/135‑1 (MQ=255)
                     tGCAATTTCCTGCCGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCt                                                                                                 >  5:298951/1‑135 (MQ=255)
                     tGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATcc                                                                                                  >  5:271468/1‑134 (MQ=255)
                     tGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCt                                                                                                 <  7:104913/135‑1 (MQ=255)
                        aaTTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTac                                                                                               <  3:209100/134‑1 (MQ=255)
                               tGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACACACCgtg                                                                                       >  2:153643/1‑135 (MQ=255)
                                                            cTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACTCCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAg                                                          <  7:136925/135‑1 (MQ=255)
                                                            cTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACTCCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAg                                                          <  7:133383/135‑1 (MQ=255)
                                                            cTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAg                                                          <  5:33367/135‑1 (MQ=255)
                                                             tCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAg                                                          >  5:147599/1‑134 (MQ=255)
                                                                 aaaaaCAATCACACATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACGAATCCCAAGGAATTATCCTACAAACCGTGTCTACGCCCGTCTTTTTGTCCGGGGAAAGCCAgg                                                     <  7:132891/135‑2 (MQ=255)
                                                                           acaAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGc                                            >  5:68550/1‑134 (MQ=255)
                                                                            caAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTacac                                                                                             >  1:39780/1‑84 (MQ=255)
                                                                            caAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTacac                                                                                             <  2:39780/84‑1 (MQ=255)
                                                                            caAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAg                                                          <  8:249150/119‑1 (MQ=255)
                                                                            caAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAg                                                          >  7:249150/1‑119 (MQ=255)
                                                                            caAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCt                                           <  6:298951/134‑1 (MQ=255)
                                                                                        ttCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGc                                <  5:272165/133‑1 (MQ=255)
                                                                                                tGGGTCCTACTTCCCCGCGAAACATATACTACGTTCACACCAATCCCAAGGAAATATCCTCCACACCGAGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTACGCTCACCAGGTCGCTGTCGGt                         >  1:6262/1‑132 (MQ=255)
                                                                                                          ttCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCggg             <  1:153643/134‑1 (MQ=255)
                                                                                                                 cgAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGaaa     >  2:52220/1‑135 (MQ=255)
                                                                                                                 cgAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGaaa     <  2:157029/135‑1 (MQ=255)
                                                                                                                 cgAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATAGGGTGTGGaaa     >  2:138594/1‑135 (MQ=255)
                                                                                                                   aaCCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAAcc   <  5:208411/135‑1 (MQ=255)
                                                                                                                    aCCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAAccc  <  6:225007/135‑1 (MQ=255)
                                                                                                                                     cacCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAg                                                          >  2:205504/1‑62 (MQ=255)
                                                                                                                                     cacCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAg                                                          <  1:205504/62‑1 (MQ=255)
                                                                                                                                      |                                                                                                                    
CCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCC  >  KT2440/5982826‑5983076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: