Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | KT2440 | 5,982,960 | A→T | 11.8% | intergenic (+419/‑270) | kefC_3 → / → CJ019_05604 | Glutathione‑regulated potassium‑efflux systemprotein KefC/hypothetical protein |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | KT2440 | 5,982,960 | 0 | A | T | 11.8% | 90.3 / 5.3 | 34 | intergenic (+419/‑270) | kefC_3/CJ019_05604 | Glutathione‑regulated potassium‑efflux systemprotein KefC/hypothetical protein |
Reads supporting (aligned to +/- strand): ref base A (11/19); new base T (2/2); total (13/21) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 6.27e-01 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.32e-01 |
CCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCC > KT2440/5982826‑5983076 | ccAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTcaca > 6:275370/1‑135 (MQ=255) aCCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTTGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATc < 3:40610/135‑1 (MQ=255) aCCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATc < 6:282826/135‑1 (MQ=255) aaGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACTCCAATCCCAAg > 8:136925/1‑134 (MQ=255) aaGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACTCCAATCCCAAg > 8:133383/1‑134 (MQ=255) aaGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAg < 7:73461/134‑1 (MQ=255) aGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAg < 1:290317/133‑1 (MQ=255) aGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAg > 2:290317/1‑133 (MQ=255) aGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGa < 1:196468/135‑1 (MQ=255) aGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGa < 1:74241/135‑1 (MQ=255) ccTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAACAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATc < 8:132080/135‑1 (MQ=255) tGCAATTTCCTGCCGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCt > 5:298951/1‑135 (MQ=255) tGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATcc > 5:271468/1‑134 (MQ=255) tGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCt < 7:104913/135‑1 (MQ=255) aaTTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTac < 3:209100/134‑1 (MQ=255) tGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACACACCgtg > 2:153643/1‑135 (MQ=255) cTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACTCCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAg < 7:136925/135‑1 (MQ=255) cTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACTCCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAg < 7:133383/135‑1 (MQ=255) cTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAg < 5:33367/135‑1 (MQ=255) tCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAg > 5:147599/1‑134 (MQ=255) aaaaaCAATCACACATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACGAATCCCAAGGAATTATCCTACAAACCGTGTCTACGCCCGTCTTTTTGTCCGGGGAAAGCCAgg < 7:132891/135‑2 (MQ=255) acaAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGc > 5:68550/1‑134 (MQ=255) caAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTacac > 1:39780/1‑84 (MQ=255) caAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTacac < 2:39780/84‑1 (MQ=255) caAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAg < 8:249150/119‑1 (MQ=255) caAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAg > 7:249150/1‑119 (MQ=255) caAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCt < 6:298951/134‑1 (MQ=255) ttCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGc < 5:272165/133‑1 (MQ=255) tGGGTCCTACTTCCCCGCGAAACATATACTACGTTCACACCAATCCCAAGGAAATATCCTCCACACCGAGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTACGCTCACCAGGTCGCTGTCGGt > 1:6262/1‑132 (MQ=255) ttCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCggg < 1:153643/134‑1 (MQ=255) cgAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGaaa > 2:52220/1‑135 (MQ=255) cgAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGaaa < 2:157029/135‑1 (MQ=255) cgAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATAGGGTGTGGaaa > 2:138594/1‑135 (MQ=255) aaCCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAAcc < 5:208411/135‑1 (MQ=255) aCCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAAccc < 6:225007/135‑1 (MQ=255) cacCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAg > 2:205504/1‑62 (MQ=255) cacCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAg < 1:205504/62‑1 (MQ=255) | CCAGGCACCTGGAAGCCATCCTGCAATTTCCTGACGATCACCTCGCCGCCATGACGCGAACTCACAAAAACCATCACAAATCGTCTAATTCTCAGCTGGGTCCTACTTCCCCGCGAACCATTTCCTACGTTCACACCAATCCCAAGGAATTATCCTACACACCGTGTCGACGCCCGTCTGATTGTCCGGGGAAAGCCAGCTCTCTAGGCTGACCAGGTCGCTGTCGGTTCAGCGATCGGGTGTGGAAACCC > KT2440/5982826‑5983076 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |