Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	KT2440	5,757,239	(A)6→5	coding (781/834 nt)	CJ019_05400 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	5,757,234	0	A	.	92.9%	109.6 / 1.5	29	coding (776/834 nt)	CJ019_05400	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base . (19/7);  minor base T (0/2);  total (19/10)
Fisher's exact test for biased strand distribution p-value = 9.52e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCG‑TGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTT  >  KT2440/5757110‑5757351                                                                                                                              |                                                                                                                      gcgcgcGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCG‑TGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAAcgccg                                                                                                             >  7:257367/1‑134 (MQ=255)       gAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCG‑TGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGGAGCGG‑AAAAACGCCGCcagcag                                                                                                      >  7:173060/1‑135 (MQ=255)         aCAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCG‑TGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCcagcag                                                                                                      >  7:284247/1‑133 (MQ=255)                 cggCCGACGTGCCCAAGGTGTTGAAGACCTTCTAGCAACCCCTCGCCCGCCAGGCCCG‑TTAAGCTGAAGAGGCACGAAAGAAGGCCCTGGTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTgg                                                                                            <  8:284247/134‑1 (MQ=255)                     cGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCG‑TGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgccgc                                                                                        >  1:168855/1‑135 (MQ=255)                      gACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCG‑TGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgccgcc                                                                                       >  7:136492/1‑135 (MQ=255)                      gACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCG‑TGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGgccgcc                                                                                       >  7:53182/1‑135 (MQ=255)                                 ggTGTTGAACACCACCGAGCAAACCCTCGCCCGCCCGGCCCG‑TCACGCTGAAGACGCACGCACGAAGCCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCcg                                                                            <  8:76680/135‑1 (MQ=255)                                   tCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCG‑TGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAACCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCcgcg                                                                          <  8:249337/135‑1 (MQ=255)                                   gcTTGAAGACCATCGAGGAAACCCTCGCCCCCCCGGCCCG‑TGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCcgcg                                                                          <  8:257367/134‑1 (MQ=255)                                        acgACCAGCGCGGAACCCCGCGCCCCCCCGGCCCG‑GGACGCTGACGAGGCCCGCAACACGGCCCTGCTGCCGCACCAGGAAG‑GGTAAAACCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCgg                                                                     <  6:92368/133‑1 (MQ=255)                                          gACCATCGAGGAAACCCGCGCCCGCCCGGCCCG‑TGAAGCTGAAGAGCCACGCAAGACGGCCCTGCGGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGc                                                                   <  8:136492/135‑1 (MQ=255)                                          caCCATCCCGCAAACCCGCGCCCGCCCGGCCCG‑GGACGCTGATGGGGCCCGCACCACGGCCCTGCTGGCGCAGCAGGATGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGc                                                                   <  8:254978/134‑1 (MQ=255)                                                cGAGGAAACCCTCGCCCGCCAGGCCCG‑TGAAGCTGAAGAGGCACGCAAGAGGGCCCTGCTGGCGCAGCCGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCCGGCCCGTgc                                                             >  1:221830/1‑134 (MQ=255)                                                cGAGGAAACCCTCGCCCGCCAGGCCCG‑TGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTgc                                                             >  6:64055/1‑134 (MQ=255)                                                cGAGGAAACCCTCGCCCGCCAGGCCCG‑TGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGa                                                             >  7:105192/1‑135 (MQ=255)                                                       aCCCTCGCCCGCCAGGCCCG‑TGAAGCTGAAGAGGCACGCAATAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCAGCCCGCGACCGCGACCGTGAGCCTGCa                                                      >  5:137405/1‑135 (MQ=255)                                                          cTCGCCCGCCAGGCCCG‑TGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTTCCCGCGCCCGGGCCCGGGAGCCAGCagag                                                   >  8:127099/1‑135 (MQ=255)                                                          cTCGCCCGCCAGGCCCG‑TGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCagag                                                   >  3:89224/1‑135 (MQ=255)                                                                 cgcctgCCCGTTGAACCTGTAGAGCCCCGCATGAAGGCCCGGCGGGCGCAGCCTGAAG‑GGTAAAACCGCCGCCAGCAGGCAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTg                                             <  4:252352/130‑1 (MQ=255)                                                                         cG‑TGAAGCTGAAGAGGCACGAACGACGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAACCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAAccgccg                                    <  7:16642/135‑1 (MQ=255)                                                                               aGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGaaaaa                               >  8:21267/1‑135 (MQ=255)                                                                                             gCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCAccc                 >  8:272862/1‑135 (MQ=255)                                                                                              cAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCt                >  2:680/1‑135 (MQ=255)                                                                                              cAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCt                >  4:80063/1‑135 (MQ=255)                                                                                                 gaagGCCCTGCTGGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCgg             >  3:48292/1‑135 (MQ=255)                                                                                                  ccgccccGGCGGGCCCACCAGGAAGC‑GCAAACCCGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCGGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCggg            <  1:165053/131‑1 (MQ=255)                                                                                                          gctgGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGt    >  4:76771/1‑135 (MQ=255)                                                                                                            tgGCGCAGCAGGAAGCGG‑AAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGttt  >  3:279461/1‑135 (MQ=255)                                                                                                                              |                                                                                                                      GCGCGCGAACAGGACCAGGCCGACCTGGCCAAGGTCTTGAAGACCATCGAGGAAACCCTCGCCCGCCAGGCCCG‑TGAAGCTGAAGAGGCACGCAAGAAGGCCCTGCTGGCGCAGCAGGAAGCGGAAAAAACGCCGCCAGCAGGAAGCGCTGGCCGCCGCCGCTGCCCGCGACCGGGCCCGTGAGCCTGCAGAGCCAGTGGAACCGCCGAAAAAGGCCCGTACCACCCTCGGGCCGATGGTTT  >  KT2440/5757110‑5757351

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑