| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | KT2440 | 120,983 | (T)9→8 | intergenic (+280/+92) | polA → / ← engB | DNA polymerase I/putative GTP‑binding protein EngB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 120,975 | 0 | T | . | 98.0% | 216.7 / ‑1.9 | 49 | intergenic (+272/+100) | polA/engB | DNA polymerase I/putative GTP‑binding protein EngB |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base . (34/14); minor base G (0/1); total (34/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.06e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.46e-01 | |||||||||||
TCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTTCCATC > KT2440/120872‑121105 | tCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTACCCCCTCCCCATACGAAGTCCTGGG‑TTTTTTTTGCACTGAATTTGCCGGGAGCGGGt > 6:151126/1‑135 (MQ=255) tATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGa > 5:93803/1‑135 (MQ=255) tATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGa < 5:207722/135‑1 (MQ=255) tgttgtGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt > 1:92071/1‑135 (MQ=255) gttgtGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt < 4:92415/134‑1 (MQ=255) gttgtGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAAt < 8:280887/134‑1 (MQ=255) tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGCCTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGGGATGt > 5:199561/1‑134 (MQ=255) tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGCACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGagggc > 6:46135/1‑131 (MQ=255) tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCGGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGt > 1:242310/1‑134 (MQ=255) tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGTAGCGGGTTTTCTCGCGAATGCGATGTc > 6:130257/1‑135 (MQ=255) tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGt > 1:170510/1‑134 (MQ=255) tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGt > 6:304542/1‑134 (MQ=255) tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGt < 8:37711/134‑1 (MQ=255) tAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTc > 2:260304/1‑135 (MQ=255) tAAGTGTTGGCAGATATCTGGACCCCGACCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGACCGGAATTTGCCGGGAGCGGGATGTCTCGCGAATGCGATGt > 8:61477/1‑134 (MQ=255) aGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGa > 1:265504/1‑135 (MQ=255) aGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACATCCCCCTCCCCATACGAAGACCGGGG‑TTTTTTTTGACCGGTATTTGCCTGGAGCGGGTTTTCTAGCGAATGAGATGTCGa > 7:134701/1‑135 (MQ=255) gtTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAAt > 3:212311/1‑134 (MQ=255) cTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCAtt > 6:129214/1‑135 (MQ=255) cTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCAt < 3:211313/134‑1 (MQ=255) gCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTaa < 2:260044/135‑1 (MQ=255) ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGTGCGGGTTTTCTCGCGAATGCGATGTCGATTACACCGCCGCCTTCGCGGGTCAAc > 2:129504/1‑135 (MQ=255) ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAAc > 7:218989/1‑135 (MQ=255) ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAAc > 5:89544/1‑135 (MQ=255) ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAAATTGACGGGAGCGGGTTTTCTCGCCAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAAc > 1:262377/1‑135 (MQ=255) ccTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCAGACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTGTTCTCGCGAATGCGATGTCGAATACACCGCCGCAGTCGCGGGTAAAc > 8:221041/1‑135 (MQ=255) ccGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAg < 2:170510/135‑1 (MQ=255) cGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAgg > 2:61840/1‑135 (MQ=255) cGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTAGAATACACCGCCGCATTCGCGGGTAAGACCGGGTCAgg > 5:150709/1‑135 (MQ=255) cGGACTTGGACCCCGAACTT‑CCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGCATGCGATGTCGAATACACCGCCCCCTTCGCGGGTAAACCCGGGTCAGgc > 5:243209/1‑135 (MQ=255) ggACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgc < 7:11147/135‑1 (MQ=255) ggACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgc > 8:194333/1‑135 (MQ=255) ggACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgc < 1:223931/135‑1 (MQ=255) ggACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgc < 1:228548/135‑1 (MQ=255) gACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGgc > 3:82849/1‑134 (MQ=255) aCTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGc < 1:232433/88‑1 (MQ=255) aCTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGc > 2:232433/1‑88 (MQ=255) ccccGAACTTCCCCCTCCCCATACCAAGTCCGGGG‑TTTTTTTTGCCCGGAATTCGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACCCCGCCGCAGTCGCGGGTAAACCCGGGTCGGGCGCAACCCa > 5:144259/1‑134 (MQ=255) cccGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTACACCCGGGTCAGGCGCAcaccac > 7:160183/1‑134 (MQ=255) cccGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCAc > 5:257650/1‑134 (MQ=255) cccGAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCAc > 3:225593/1‑134 (MQ=255) gAACTTCCCCCTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTaaa < 8:218989/135‑1 (MQ=255) cgtCCCCCTCCCCATCCCATG‑CCGGGGGTTTTTTATGCCCGGAATTTGCCGGTAGCGGGTTTTCGCGCTAATGCTATGTCGAATGCACCGCCTCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAAc < 4:218232/131‑1 (MQ=255) cccTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGc < 7:194333/135‑1 (MQ=255) ccTCCCCATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCCGGCGCAAACCACTAAACGACCGGcg > 6:196136/1‑134 (MQ=255) cATACGAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTACACCCGGGTCAGGCGCAAACCACTACACGACCGGCTTGTCtt > 4:173433/1‑135 (MQ=255) gAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTc > 8:6346/1‑128 (MQ=255) gAAGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTc < 7:6346/128‑1 (MQ=255) aaGTCCGGGG‑TTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGtt > 6:250832/1‑135 (MQ=255) ccggggTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCTATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACAAAACGACCGGCTTGTCTTCCAGTtccat < 6:164896/133‑1 (MQ=255) cggggTTTTTTTTGCCCGTAATTGGCCGGGTGGGGGTTTTCGCGCGAATGCTTTGTCTAATACACCGCGGCATTCGGGGGTAAACCCGGGTCAGGCGAAAACCACAAAACGACCGGCTTGTCTTCCAGTtccatc < 4:72942/134‑1 (MQ=255) cggggTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTtccatc > 7:126076/2‑135 (MQ=255) cggggTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTtccat > 3:173825/2‑134 (MQ=255) cggggTTTTTTTTGCCCGCAATTTGCCGGGAGGGGGTTTTCTGGCGATTGCGATGTCTAATACACCGCCGCATTCGCGGGTAAACCGGGGTCAGGCGAAAACAACTACACGACCGGCTTGTCTTCCAGTtccatc < 8:229766/134‑1 (MQ=255) | TCGATGCTCCTATGTTGTGTTAAGTGTTGGCAGATATCTGGACCCCGCCCTAGCGGTCCGGACTTGGACCCCGAACTTCCCCCTCCCCATACGAAGTCCGGGGTTTTTTTTTGCCCGGAATTTGCCGGGAGCGGGTTTTCTCGCGAATGCGATGTCGAATACACCGCCGCATTCGCGGGTAAACCCGGGTCAGGCGCAAACCACTAAACGACCGGCTTGTCTTCCAGTTCCATC > KT2440/120872‑121105 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |