Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24401,524,7320TG52.0% ‑1.9 / 11.9 25Q67P (CAG→CCG) CJ019_01439hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (4/8);  new base G (0/13);  total (4/21)
Fisher's exact test for biased strand distribution p-value = 3.91e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.88e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCAGTGCTCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTATCGGTATCT  >  KT2440/1524608‑1524857
                                                                                                                            |                                                                                                                             
gctgTGCTCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGGGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCCGCGGGCGCACCTGGCGCAGCAGGGGGGAAGGGGGCTGGGCggggg                                                                                                                      <  2:234927/131‑1 (MQ=255)
     gCTCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCt                                                                                                                               <  7:113803/120‑1 (MQ=255)
     gCTCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCt                                                                                                                               <  7:110518/120‑1 (MQ=255)
     gCTCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCt                                                                                                                               >  8:110518/1‑120 (MQ=255)
     gCTCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCt                                                                                                                               >  8:113803/1‑120 (MQ=255)
       tCGCGGGCAGCCGGTTGCAACTGGCTTTCCCGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCGTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTg                                                                                                              >  2:31324/1‑135 (MQ=255)
       tCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTg                                                                                                              >  1:133287/1‑135 (MQ=255)
         gcgGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTa                                                                                                            >  4:163376/1‑135 (MQ=255)
                 ccGGTTGCAACGGGCTTTCCAGCAGGCGCTGCATGTGCTCCAGGCGGGGGGCCTGGTTCCGCCGCGGGCGCAGGGGGCGCACCTGGCGCCGCCGGGCGTAAGGGGGCTGGGGGGGGGAGGGTTTGTAGGCCATgg                                                                                                    <  6:54226/135‑1 (MQ=255)
                 ccGGTGGCAAATGGCTTTCCAGCTGGCGCTCCACGGGCGCCAGGCGCGCGGCCGGGTGCAGCAGCAGGGGAAGCGGGCGACCCTGGCGCAGCAGGGCGTAAGGGGGCTGGGCGGGGGAGGGTTTGTAGGCCATgg                                                                                                    <  8:232709/135‑1 (MQ=255)
                      tGCAAGTGGCTTTCCCGCTGGCGCTGCAAGGGCGCCAGGCGGTGGGCCGGGTGCAGCAGCAGGCGCTGCGGGCGCACCTGGCGCAGCAGGGCGGATGGTGGCTGGGGGGGGGAGGGGTTGTAGGCCATGGGGGTa                                                                                               <  2:236409/135‑1 (MQ=255)
                      tGCAACGGGCTTTCCAGCCGGCGCTGCACGGGCGCCCGGCGCGCGGCGTGGTTCAGCCGCCGGCGCAGCGGGCGCACCTGGCGCAGCAGGGGGTAAGGTGGCTGGGGGGGGGAGGGGTTGTAGGCCATGGGGGTa                                                                                               <  5:298373/135‑1 (MQ=255)
                             gggtttCCAGCAGGCGGTGCAAGGGCGCCAGGCGCTCGGCCTGGTTCACCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGTAAGGGGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGa                                                                                        <  2:20412/132‑1 (MQ=255)
                             ggCTTCCCAGCAGGCGCTGCATGTGCTCCAGGCGCTCGGCAGGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCTGCAGGGCGGAAGTGGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGa                                                                                        <  5:2255/135‑1 (MQ=255)
                                 ttCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTg                                                                                    >  3:40515/1‑135 (MQ=255)
                                     agccgGCGCTGCAAGGGCTCCCGGCGCTCGGCCTGGTTCAGCAGCCGGCGCAGCGGGCGCACCGGGCGCAGCAGGGCGGAAGGGGGCGGGGGGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGt                                                                                <  1:193078/135‑1 (MQ=255)
                                      gctggCGCTGCACGTGCTCCCGGCGCTGGGCCGGGTGCAGCAGCTGGCGCTGGGGGCGCACCTGGCGCTGCGGGGGGGAAGGTGGCGGGGGGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTg                                                                               <  5:217817/132‑1 (MQ=255)
                                       ccggCGCGGCATGGGCGCCCGGCGCGCGGCCTGGTTCAGCCGCCGGCGCCGGGGGCGCACCGGGCGCAGCCGGGCGCAAGGGGGCGGGGGGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTgg                                                                              <  2:44976/133‑1 (MQ=255)
                                       cagGCGCGTCACGGGCTCCAGGGGGGCGGCCGGTTTCCGCCGCAGGGGCAGGGGGCGCCCGGGGCGCAGCAGGGCGGATGGTGGCGGGGCGGGGGAGGGGTTGTAGGCCATGGGGGGACGCGTGAAGTGACGTgg                                                                              <  6:167250/135‑1 (MQ=255)
                                               gaaaGGGCGCCCGGCGCGGGGCCGGGTGCAGCCGCCGGCGCAGGGGGCGCACCGGGCGCCGCCGGGCGCATGGGGGCGGGGCGGGGGCGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTc                                                                      <  2:8492/133‑1 (MQ=255)
                                                  tgTGCTCCCGGCGCGCGGCGTGGTGCAGCCGCAGGCGCCGCGGGCGCACGTGGCGCTGCCGGGCGGAAGGGGGCGGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTaa                                                                   <  2:4529/134‑1 (MQ=255)
                                                      cTCCAGGCGCGGGGCGTGGTTCCGCCGGCGGCGCCGCGGGCGCACCTGGCGCTGGTGGGGGTAGGGGGGCGGGGGGGGGGCGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACAtc                                                               <  2:133287/135‑1 (MQ=255)
                                                                                 cagGCGCAGCGTGCTCACCGGGCGACGCAGGGCGGAAGGTGCCGGGGCGGGTGCGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTATCATCTCCGTTGCCTGGGCCGGCCTCTTcgcg                                    <  7:31913/135‑1 (MQ=255)
                                                                                         gCGGGCGCACCTGGCGCAGCCGGGCGGAAGGGGGCGGGGCGGGGGAGGGTTTGTAGGCCAGGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAAcc                            <  4:68954/135‑1 (MQ=255)
                                                                                          cGGGCGCCCGGGGCGCCGCGGGGGGTTAGGTGGGGGGGGGGGGCCGGGTTGGCAGGCCAGGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAAccc                           <  8:248174/135‑1 (MQ=255)
                                                                                                        gcagccGGGCGTAAGGGGGCGGGGGGGGGGCGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGt             <  3:280912/135‑1 (MQ=38)
                                                                                                          tgccgggCGGAAGGTGGGTGGGCGGGGGTGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTa           <  7:184730/131‑1 (MQ=255)
                                                                                                             tgggCGGAAGGTGGCGGGGCGGGGGCGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTATCg        <  1:128722/134‑1 (MQ=255)
                                                                                                                   gAAGGGGGCGGGGCGGGGGTGGGTTTGTAGGCCATGGGGGTACGCGTGAAGGGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTATCGGTATCt  <  6:157083/135‑1 (MQ=255)
                                                                                                                            |                                                                                                                             
GCAGTGCTCGCGGGCAGCCGGTTGCAACTGGCTTTCCAGCAGGCGCTGCAAGTGCTCCAGGCGCTCGGCCTGGTTCAGCAGCAGGCGCAGCGGGCGCACCTGGCGCAGCAGGGCGGAAGGTGGCTGGGCGGGGGAGGGTTTGTAGGCCATGGGGGTACGCGTGAAGTGACGTGGGCACAGTCTAACATCTCCGTTGCCTGTGCCGGCCTCTTCGCGGGGGAACCCGCTCCTGCCAGGGTTATCGGTATCT  >  KT2440/1524608‑1524857

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑