| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 1,974,160 | 0 | T | G | 52.4% | 5.9 / 18.7 | 21 | intergenic (+42/‑258) | rpsA/ihfB | 30S ribosomal protein S1/Integration host factor subunit beta |
| Reads supporting (aligned to +/- strand): ref base T (4/6); new base G (11/0); total (15/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.87e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.30e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACA‑CGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAGGCGCCACTGGCCTATCTAAGCGGTA > KT2440/1974026‑1974289 | gAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCt > 3:61976/1‑135 (MQ=255) cTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGCCCCTCGGGTCGCCCGTGTTTTTGCTGcgcg > 3:213684/1‑135 (MQ=255) gAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCGTTTTTTTTCTCCGCGAc > 5:51165/1‑135 (MQ=255) aaaCGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCGCGTTTTTTTTCTACGCGAcccc > 7:292069/1‑133 (MQ=255) aaaCGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCGTTTTTTTGCTCGGCGGcccc > 2:269428/1‑133 (MQ=255) aaaCGCTACGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGCCCCTCGGGTCGCCAGGATTTTTGCTCCGCGCCCAg > 3:51860/1‑135 (MQ=255) ggtggtgcCCACCCCCCCTTGGCGGCGCTGCGGCGCGACCAAAGGCCCACCCACACTTGCGTGGTGTTTGCTCGGAAAAACGGGCGCCCCTCGGGGCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGa < 4:213684/130‑1 (MQ=255) gggggcCTACACCACCATGGCGGCGGTGCTGCGCTAAGCAATGGCCAAGCAGAACTGTGTTGTGTTTGTGCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATg < 8:292069/131‑1 (MQ=255) gcCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCGTTTTTTTTCTCCGCGCCCACGGGGGGCGGCGGATGGGAc > 6:147032/1‑135 (MQ=255) ccaTGGCGGCGCTGCGGCGCGAAGCAATGGCCAAGCATACCTGAGTACTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAaca < 2:190847/135‑1 (MQ=255) caTGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCGTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCCCTGCTGGAaca‑c > 4:203085/1‑135 (MQ=255) ctgctgCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCGCGTTTTTTTGCTCCGCGCCCAGGTGGTGCGGGGGATGGGCCTGCTGGAACA‑CGCCCGcgtac > 4:48758/1‑131 (MQ=255) ctgctgCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGCCCAGGTGGTGGGGCGGATGGCGCTGCTGGAACA‑CGCCAGCGTCa > 3:206985/1‑135 (MQ=255) tgctgcGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGCCCCTCGGGTCGCCCGTTTTTTTTGTCCGCGCCCAGGTGGTGCGGCGGGTGGCCCGGCTGGGACA‑CCCCCGCGTCac > 6:87066/1‑134 (MQ=255) gAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGCCCAGGTGGTGCGGCGGATGGCCCGGCTGGAACA‑CGCCAGCGTCAGGCGGGGGCAGCCCGGTGTAGGcgcg > 4:290685/1‑135 (MQ=255) gAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCGTTTTTTTGCTCCGCGCCCAGGTGGTGCGGCGGATGGCCCTGCTGGAACA‑CGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCg > 7:242107/1‑135 (MQ=255) tGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACA‑CCCCAGCTTCAGGCTGGCGCAGCCCGGTGTAGGAGCGGGGTCAccccc > 8:79456/1‑133 (MQ=255) ttGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTTATACGCGACCAGGTGGTGCGGCCGATGGCACAGGAGGAACA‑CCCCCGCGTCAGGCTGGCGAAGACCCTGGTATGGCCCGGTTCAccccccaa > 8:134707/1‑130 (MQ=255) gTAAAAAGGGCGACCCTCGGGTCGCCCGTTTTTTTGCTCCGCGCCCAGGTGGGGCGGCGGATGGCACTGCTGGGACACCCCCCGCTTCGGGCGGGCGACGCCCGTTGTTGGCGCGGGTGCCCCCGCGAACCAGgc > 2:85998/1‑135 (MQ=255) aacgggCGCCGCTCGGGCGGCCTTTTTTTTTGCTCCGCGACCTGGGGGGGCGGGGTAGGGCACTGCTGTAACC‑CGCCAGCTACTGGGGGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAGGCGCCAc < 4:206985/132‑1 (MQ=19) cGCCTTTTTTTTTGCGCCGCGCCCGGGGGGGGCGGCGGAGGGCCGTGCGGCAACC‑CCCCAGCTGCCGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAGGCGCCACTGGCCTATCTAAGCGGTa < 6:95837/135‑1 (MQ=25) cGCCTTTTTTTTGGCCCCGCGCCCCGGGGGGGGGGGGTTTGCCACTGCGGCAACA‑CCCCAGCTGCGGGCGGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAGGCGCCACTGGCCTATCTAAGCGGTa < 7:79456/135‑1 (MQ=19) | GAAGCTATCCAGAGCCTGAAAAACGCTCCGGAAGCTGCTGCCGACACCACCATGGCTGCGCTGCTGCGCGAAGCAATGGCCAAGCAGAACTGAGTTCTGTTTGATCGGTAAAAAGGGCGACCCTCGGGTCGCCCTTTTTTTTGCTCCGCGACCAGGTGGTGCGGCGGATGGCACTGCTGGAACA‑CGCCAGCTTCAGGCTGGCGAAGCCCGTTGTAGGAGCGGGTTCACCCGCGAAGCAGGCGCCACTGGCCTATCTAAGCGGTA > KT2440/1974026‑1974289 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |