Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,151,9570GT23.3% 50.0 / 14.5 43intergenic (+69/‑119)acpP_1/fabF_1Acyl carrier protein/3‑oxoacyl‑[acyl‑carrier‑protein] synthase 2
Reads supporting (aligned to +/- strand):  ref base G (8/25);  new base T (10/0);  total (18/25)
Fisher's exact test for biased strand distribution p-value = 2.28e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.22e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AGATCACTACCGTTCAAGCTGCCATCGACTACGTCAAAGCCCACCAGGCCTAAGACGCTACAGTCGACGTTTCTTGTCGGGGAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCG‑CATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTG‑TTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTC  >  KT2440/2151836‑2152080
                                                                                                                         |                                                                                                                             
agaTCACTACCGTTCAAGCTGCCATCGACTACGTCAAAGCCCACCAGGCCTAAGACGCTACAGTCGACGTTTCTTGTCGGGGAAAACCGCACTGCCTTCGCTGGCGTGGGGTTTTTTATTTTGGCGCGCCTCG‑Ca                                                                                                                 >  4:29827/1‑135 (MQ=255)
       tACCGTTCAAGCTGCCATCGACTACGTCAAAGCCCACCAGGCCTAAGACGCTACAGTCGACGTTTCTTGTCGGGGAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTGATTGCGAGCCCCTCG‑CAgttttga                                                                                                          >  7:47844/1‑133 (MQ=255)
       tACCGTTCAAGCTGCCATCGACTACGTCAAAGCCCACCAGGCCTAAGACGCTACAGTCGACGTTTCTTGTCGGGGAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTTCGAGCGCCTCG‑CATTTTTCa                                                                                                          >  4:277784/1‑135 (MQ=255)
       tACCGTTCAAGCTGCCATCGACTACGTCAAAGCCCACCAGGCCTAAGACGCTACAGTCGACGTTTCTTGTCGGGGAAAACCCCCCCGGCGTCGCGGGCGGGGGGGTTTTTTTTTTTGACCGCCCCG‑CCatttt                                                                                                            >  6:173577/1‑133 (MQ=255)
               aaGCTGCCATCGACTACGTCAAAGCCCACCAGGCCTAAGACGCTACAGTCGACGTTTCTTGTCGGGGAAAACCGCACTGCCTTCGCTGGCGTGCGGGTTTTTATTTGCGAGCGCCTCG‑CATTTTTAAggcggcgt                                                                                                  >  4:197963/1‑133 (MQ=255)
               aaGCTGCCATCGACTACGTCAAAGCCCACCAGGCCTAAGACGCTACAGTCGACGTTTCTTGTCGGGGAAAAACGCACTTCCTTCGCTTGCGTGCGGTTTTTTATTTGAGCTAGCCTAG‑CATTTTTAAggaggcat                                                                                                  >  4:286312/1‑133 (MQ=255)
               aaGCTGCCATCGACTACGTCAAAGCCCACCAGGCCTAAGACGCTACAGTCGACGTTTCGTGTCGGGGAAAAACGCCCTTCCCTAGCAGGCGGGCGGTTGTTTATTTTCCCTCGCCTCGCCATTTTTAAgcgcc                                                                                                     >  6:273806/1‑129 (MQ=255)
                      cATCGACTACGTCAAAGCCCACCAGGCCTAAGACGCTACAGTCGACGTTTCTTGTCGGGGTAAAGCGCTCTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCG‑CATTTTTCAGGCGGCTTCGTTCa                                                                                            <  6:73681/134‑1 (MQ=255)
                      cATCGACTACGTCAAAGCCCACCAGGCCTAAGACGCTACAGTCGACGTTTCTTGTCGGGGAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCG‑CATTTTTCAGGCGGCTTCGTTCa                                                                                            <  3:29827/134‑1 (MQ=255)
                       aTCGACTACGTCAAAGCCCACCAGGCCTAAGACGCTACAGTCGACGTTTCTTGTCGGGGAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCG‑CATTTTTCAGGCGGCTTCGTTCAcc                                                                                          <  7:203039/135‑1 (MQ=255)
                         cGACTACGTCAAAGCCCACCAGGCCTAAGACGCTACAGTCGACGTTTCTTGTCGGGGAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTGTTTGCGAGCGCCTCG‑GATTTTTGAGGCGGGGTGGTTGACcgc                                                                                        >  7:4869/1‑135 (MQ=255)
                                  cAAAGCCCACCAGGCCTAAGACGCTACAGTCGACGTTTCTTGTCGGGGAAAACCGCACTGCCGTCGCTGGCGTGGGGGTTTTTGTTTTCGAGCGCCGCG‑CCTTTTTAAGGCGGGTTGGTTGACCGCGCCCCAaga                                                                               >  1:158470/1‑135 (MQ=255)
                                    aaGCCCACCAGGCCTAAGACGCTACAGTCGACGTTTCTTGTCGGGGAAAACCGCACTGCCTTCGCTGGCGTGCGGGTTTTTCTTTGCGAGCGCCGCG‑CATTTTTAAGGCGGCTTCGTTCACCGCGCACCAagcg                                                                              >  8:300678/1‑132 (MQ=255)
                                                     gACGCTACAGTCGACGTTTCTTGTCGGGGAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCG‑CATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTG‑TTGCCATTTCATTc                                                           <  5:25729/135‑1 (MQ=255)
                                                     gACGCTACAGTCGACGTTTCTTGTCGGGGAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCG‑CATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCATG‑TTGCCATTTCAt                                                             <  5:47206/133‑1 (MQ=255)
                                                      aCGCTACAGTCGACGTTTCTTGTCGGGGAAAAACGCACTGCCTTCGCTGGCGTGGGGGTTTTTTTTTTCGCTCGCCCCG‑CATTTTTAAGGGCGGGTAGTTTACCGCGCACCAAGATCCTGTTTGCCAttaattac                                                           >  4:30202/1‑129 (MQ=255)
                                                            cAGTCGACGTTTCTTGTCGGGGAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTGTTTTCGAGCGCCACG‑CCTTTTTAAGGCGGGGTCGGTTAACGCCCCCCAAGAGCCAG‑TTTACATTTAATTCAAGCACt                                                    >  2:230461/1‑135 (MQ=255)
                                                            cAGTCGACGTTTCTTGTCGGGGAAAACCGCACTGCCTTCGCTGGCGTGCGGGTTTTTGTTTGGGAGCGGCGCG‑CATTTTTTAGGCGGCGTGGGTGACCGCGCCCCCAGAGCCTG‑TTTCCATTTGATTCCCGGACt                                                    >  7:104661/1‑135 (MQ=255)
                                                            cAGTCGACGTTTCTTGTCGGGGAAAACCCCACTGCCTTCGCTGGCGTGGGGGTTTTTGTTTTGGAGCGCCGCG‑CCTTTTTAACGCGGCGTGGGTTACCCCGCACCAAGAGCCTG‑TTTCCAATTAATTACAGCAca                                                    >  6:287562/1‑134 (MQ=255)
                                                             aGTCGACGTTTCTTGTCGGGGAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTTCGAGCGCCTCG‑CATTTTTCAGGCGGCTTCGGTCACCGCGCACCAAGAGCCAG‑TTTCCATTTCATTCCAGCAcg                                                    >  8:74235/1‑133 (MQ=255)
                                                              gTCGACGTTTCTTGTCGGGGAAAACCGCACTGCCTTCGCTGGCGTGCGGGTTTTTCTTTGCGAGCGCCTCG‑CATTTTTCAGGCGGCTTCGATCACCGCGCACCAAGAGCCTG‑TTGCCATTTCATTCCAGCACTTg                                                  <  4:292015/135‑1 (MQ=255)
                                                                            tCGGGGAAAACCGCACTGCCTTCGCTGGCGTGCGGGTTTTTCTTTGCGCGCGCCTCG‑CATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTG‑TTTCCATTTCATTCCAGCACTTTAATGCAAAgg                                        >  2:86166/1‑131 (MQ=255)
                                                                                     aCCGCACTGCCTTCGCTGGCGTGCTGTTTTTTCTTTGCGAGCGCCTCG‑CATTTTTCAGGCGGCTGCGTTCACCGCGCACCAAGAGCCTG‑TTGCCATTTCATTCCAGCACTTGAATGCAATGGAAAGAGACTCTGt                           <  5:273806/135‑1 (MQ=255)
                                                                                     aCCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCG‑CATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTG‑TTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGt                           <  7:121581/135‑1 (MQ=255)
                                                                                          aCTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCG‑CATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTG‑TTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGttatta                      <  5:132008/135‑1 (MQ=255)
                                                                                          aCTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCG‑CATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTG‑TTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGttatta                      <  7:300678/135‑1 (MQ=255)
                                                                                          aCTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCG‑CATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTG‑TTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGttatta                      <  3:100398/135‑1 (MQ=255)
                                                                                          aCTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCG‑CATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTG‑TTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGttatta                      <  3:286312/135‑1 (MQ=255)
                                                                                          aCTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCG‑CATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTG‑TTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGttatta                      <  3:197963/135‑1 (MQ=255)
                                                                                            tGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCG‑CATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTG‑TTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGa                    <  5:29466/135‑1 (MQ=255)
                                                                                               cTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCG‑CATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTG‑TTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGaaaa                 <  1:86166/135‑1 (MQ=255)
                                                                                               cTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCG‑CATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTG‑TTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGaaaa                 <  5:173577/135‑1 (MQ=255)
                                                                                               cTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCG‑CATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTG‑TTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGaaaa                 <  3:277784/135‑1 (MQ=255)
                                                                                               cTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCG‑CATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTG‑TTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGaaaa                 <  3:30202/135‑1 (MQ=255)
                                                                                               cTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCG‑CATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTG‑TTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGaaaa                 <  6:108378/135‑1 (MQ=255)
                                                                                                    cTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCG‑CATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTG‑TTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGgaga            <  7:74235/135‑1 (MQ=255)
                                                                                                    cTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCG‑CATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTG‑TTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGgaga            <  2:164339/135‑1 (MQ=255)
                                                                                                         gTGCGGTTTTTTCTTTGCGAGCGCCTCT‑CATTTTTCCGGCGGCTTCGTTCACCGCGCACCAAGAGCGTG‑TTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACt       <  2:57761/135‑1 (MQ=255)
                                                                                                         gTGCGGTTTTTTCTTTGCGAGCGCCTCG‑CATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTG‑TTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACt       <  6:66669/135‑1 (MQ=255)
                                                                                                         gTGCGGTTTTTTCTTTGCGAGCGCCTCG‑CATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTG‑TTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACt       <  6:204775/135‑1 (MQ=255)
                                                                                                           gCGGTTTTTTCTTTGCGAGCGCCGCG‑CATTTTTCAGGCGGCTTCGTTAACCGCGCACCAAGAGCCTG‑TTGGCATTTGATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAATGAGAGTACtgt     >  6:129703/1‑135 (MQ=255)
                                                                                                           gCGGGTTTTTCTTTTCGAGCGCCTCG‑CATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTG‑TTTCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACtgt     >  4:28379/1‑135 (MQ=255)
                                                                                                              gTTTTTTCGTTGCGAGCGCCTCG‑CATTTTTCCGGCGGCTTCGTTCACCGCGCACCAAGAGCCTG‑TTGCCATTTCATTCCAGCACTTGAAGGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTc  <  8:142767/135‑1 (MQ=255)
                                                                                                                         |                                                                                                                             
AGATCACTACCGTTCAAGCTGCCATCGACTACGTCAAAGCCCACCAGGCCTAAGACGCTACAGTCGACGTTTCTTGTCGGGGAAAACCGCACTGCCTTCGCTGGCGTGCGGTTTTTTCTTTGCGAGCGCCTCG‑CATTTTTCAGGCGGCTTCGTTCACCGCGCACCAAGAGCCTG‑TTGCCATTTCATTCCAGCACTTGAATGCAATGGCAAGAGACTCTGTTATTAGAAAAGGAGAGTACTGTGTC  >  KT2440/2151836‑2152080

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑