Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,673,7900GC24.3% 85.2 / 10.1 37intergenic (+19/+25)CJ019_03485/panShypothetical protein/Pantothenate precursors transporter PanS
Reads supporting (aligned to +/- strand):  ref base G (4/24);  new base C (9/0);  total (13/24)
Fisher's exact test for biased strand distribution p-value = 5.75e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CCGCTTGCACCATGCGGTGAAGGGGCGCACGGGGAGCGTGTCGTTCGGGTTCTTCTATGCGCCGCCGCTGAAGGTGTTGAAGCAGCAATTGCGCAGCAGGCGCAGCCAATCGTGACGCATCCCTGGCACGCCCGGCACCTG‑CCGGGCATCGGGTGAAATCAGTTCACGGTTTCACCCTTGGGCTCAGCCGCCACATGCCCGCGGCTCACCCACCAGCCAAACCCGGCCGCGGTAAAGAACATGATCAAGCTG  >  KT2440/3673657‑3673908
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ccGCTTGCACCATGCGGTGAAGGGGCGCACGGGGAGCGTGTCGTTCGGGTTCTTCTATGCGCCGCCGCTGAAGGTGTTGAAGCAGCAATTGCGCAGCAGGCGCAGCCAATCGTGACGCATCCCTGGCACGCCCgg                                                                                                                        <  5:156916/135‑1 (MQ=255)
ccGCTTGCACCATGCGGTGAAGGGGCGCACGGGGAGCGTGTCGTTCGGGTTCTTCTATGCGCCGCCGCTGAAGGTGTTGAAGCAGCAATTGCGCAGCAGGCGCAGCCAATCGTGACGCATCCCTGGCACGCCCgg                                                                                                                        <  3:184392/135‑1 (MQ=255)
ccGCTTGCACCATGCGGTGAAGGGGCGCACGGGGAGCGTGTCGTTCGGGTGCTTCTATGCGCCGCCGCTGAAGGTGTTGAAGCAGCAATTGCGCAGCAGGCGCAGCCAATCGTGACGCATCCCTGGCACGCCCgg                                                                                                                        <  6:19066/135‑1 (MQ=255)
  gCTTGCACCATGCGGTGAAGGGGCGCACGGGGAGCGTGTCGTTCGGGTTCTTCTATGCGCCGCCGCTGAAGGTGTTGTAGCAGCAATTGCGCAGCAGGCGCAGCCCATCGTGACGCATCCCTGGCACGCCCCGcc                                                                                                                      >  4:235214/1‑134 (MQ=255)
           aTGCGGTGAAGGGGCGCACGGGGAGCGTGTCGTTCGGGTTCTTCTATGCGCCGCCGCTGAAGGTGTTGAAGCAGCAATTGCGCAGCAGGCGCAGCCAATCGTGACGCATCCCTGGCACGCCCGGCACCTG‑CCggg                                                                                                            <  8:93799/135‑1 (MQ=255)
           aTGCGGTGAAGGGGCGCACGGGGAGCGTGTCGTTCGGGTTCTTCTATGCGCCGCCGCTGAAGGTGTTGAAGCAGCAATTGCGCAGCAGGCGCAGCCAATCGTGACGCATCCCTGGCACGCCCGGCACCTG‑CCggg                                                                                                            <  3:45013/135‑1 (MQ=255)
             gCGGTGAAGGGGCGCACGGGGAGCGTGTCGTTCGGGTTCTTCTATGCGCCGCCGCTGAAGGTGGTGAAGCAGCAATTTCGCAGCAGGCGCAGCCAAACGTGCCGCATCCCTGGCACGGCCCGCCCCAG‑ccccgcc                                                                                                          >  3:201739/1‑130 (MQ=255)
              cGGTGAAGGGGCGCACGGGGAGCGTGTCGTTGGGGTTCTTCTATGCGCCGCCGCTGAAGGTGTTGAAGCAGCAATTTCGCAGCAGGCGCAGCCCATCGTGCCGCCTCCCTGGCACGCCCCGCCCCGG‑CCggggca                                                                                                         >  5:161274/1‑132 (MQ=255)
              cGGTGAAGGGGCGCACGGGGAGCGTGTCGTTCGGGTTGTTCTTTGCGCCGCCGCGGAAGGTGTTGCAGCCCCAATTTCGCAGCAGGCGCAGCCAATCGTGCCGCCTCCCTGGCACGCCCCGCCCCCG‑CCCGGc                                                                                                           >  7:67454/1‑133 (MQ=255)
              cGGTGAAGGGGCGCACGGGGAGCGTGTCGTTCGGGTTCTTCTATGCGCCGCCGCTGAAGGTGTTGAAGCAGCAATTGCGCAGCAGGCGCAGCCAATCGTGACGCATCCCTGGCCCGCCCCGCCCCCG‑GCgggg                                                                                                           >  3:87109/1‑132 (MQ=255)
               ggTGAAGGGGCGCACGGGGAGCGTGTCGTTCGGGTTCTTCTATGCGCCGCCGCTGAAGGTGTTGAAGCAGCAATTGCGCAGCAGGCGCAGCCAATCGTGCCGCATCCCTGGCACGCCCGGCACCTG‑CCGGGCATc                                                                                                        >  6:100535/1‑135 (MQ=255)
               ggTGAAGGGGCGCACGGGGAGCGTGTCGTTCGGGGTCTTCTATGCGCCGCCGCTGAAGGTGTTGAAGCAGCAATTGCGCAGCAGGCGCAGCCAATCGTGCCGCATCCCTGGCACGCCCCGCCCCAG‑CCCGGccaa                                                                                                        >  5:115231/1‑132 (MQ=255)
                gTGAAGGGGCGCACGGGGAGCGTGTCGTTCGGGTTCTTCTATGCGCCGCCGCGGAAGGTGTTGAAGCCGCAATTGCGCCGCAGGCGCCGCCAATCGTGCCGCATCCCGGGCCCCCCCCGCCCCCG‑CCCGGccaag                                                                                                       >  4:36241/1‑131 (MQ=255)
                     ggggCGCACGGGGAGCGTGTCGTTCGGGTTCTTCTATGCGCCGCCGCTGAAGGTGTTGAAGCAGCAATTGCGCAGCAGGCGCAGCCAATCGTGACGCATCCCTGGCACGCCCGGCACCTG‑CCGGGCATCGGGTGa                                                                                                  <  5:73168/135‑1 (MQ=255)
                     ggggCGCACGGGGAGCGTGTCGTTCGGGTGCTTCTATGCGCCGCCGCTGAAGGTGTTGAAGCAGCAATTGGGCAGCAGGCGCAGCCAATCGTGACGCATCCCTGGCACGCCCGGCACCTG‑CCGGGCATCGGGTGa                                                                                                  <  5:264620/135‑1 (MQ=255)
                            aCGGGGAGCGTGTCGTTCGGGTTCTTCTATGCGCCGCCGCTGAAGGTGTTGAAGCAGCAATTGCGCAGCAGGCGCAGCCAATCGTGACGCATCCCTGGCACGCCCGGCACCTG‑CCGGGCATCGGGTGAAATCAGt                                                                                           <  1:165252/135‑1 (MQ=255)
                            aCGGGGAGCGTGTCGTTCGGGTTCTTCTATGCGCCGCCGCTGAAGGTGTTGAAGCAGCAATTGCGCAGCAGGCGCAGCCAATCGTGACGCATCCCTGGCACGCCCGGCACCTG‑CCGGGCATCGGGTGAAATCAGt                                                                                           <  7:245990/135‑1 (MQ=255)
                            aCGGGGAGCGTGTCGTTCGGGTTCTTCTATGCGCCGCCGCTGAAGGTGTTGAAGCAGCAATTGCGCAGCAGGCGCAGCCAATCGTGACGCATCCCTGGCACGCCCCGCCCCAG‑CCGGGCATAGGGGGCAAACAg                                                                                            >  6:142834/1‑134 (MQ=255)
                                  aGCGTGTCGTTCGGGTTCTTCTCTGCGCCGCCGCTGAAGGTGTTGAAGCAGCAATTGCGCAGCAGGCGCAGCCAATCGTGACGCATCCCTGCCACGCCCGGCACCTG‑CCGGGCATCGGGTGAAATCAGTTCACgg                                                                                     <  7:234017/135‑1 (MQ=255)
                                  aGCGTGTCGTTCGGGTTCTTCTATGCGCCGCCGCTGAAGGTGTTGAAGCAGCAATTGCGCAGCAGGCGCAGCCAATCGTGACGCATCCCTGGCACGCCCGGCACCTG‑CCGGGCATCGGGTGAAATCAGTTCACgg                                                                                     <  1:12669/135‑1 (MQ=255)
                                  aGCGGGTCGTTCGGGTTCTGCTATGCGCCGCCGCTGAAGGTGTTGAAGCAGCAATTGCGCAGCAGGCGCAGCCAATCGTGACGCATCCCTGGCACGCCCGGCACCTG‑CCGGGCATCGGGTGAAATCAGTTCACgg                                                                                     <  6:161274/135‑1 (MQ=255)
                                       gtCGTTCGGGTTCTTCTATGCGCCGCCGCTGAAGGTGTTGAAGCAGCAATTGCGCAGCAGGCGCAGCCAATCGTGACGCATCCCTGGCACGCCCGGCACCTG‑CCGGGCATCGGGTGAAATCAGTTCACGGTTTCa                                                                                <  5:168005/135‑1 (MQ=255)
                                               ggTTCTTCTATGCGCCGCCGCTGAAGGTGTTGAAGCAGCAATTGCGCAGCAGGCGCAGCCAATCGTGACGCATCCCTGGCACGCCCGGCACCTG‑CCGGGCATCGGGTGAAATCAGTTCACGGTTTCACCCTTgg                                                                         <  5:140106/134‑1 (MQ=255)
                                                       tATGCGCCGCCGCTGAAGGTGTTGAAGCAGCAATTGCGCAGCAGGCGCAGCCAATCGTGACGCATCCCTGGCACGCCCCGCCCCAGCCCGGCCAACGGG‑GAAATCAGTTACCCGTTTCACCCGATGGCGCAgccc                                                                >  1:19699/1‑134 (MQ=255)
                                                               gccgcTGAAGGTGTTGAAGCAGCAATTGCGCAGCAGGCGCAGCCAATCGTGACGCATCCCTGGCACGCCCGGCACCTG‑CCGGGCATCGGGTGAAATCAGTTCACGGTTTCACCCTTGGGCTCAGCCGCCACATGc                                                        <  2:242555/135‑1 (MQ=255)
                                                                   cTGAAGGTGTTGAAGCAGCAATTGCGCAGCAGGCGCAGCCAATCGTGACGCATCCCTGGCACGCCCgg                                                                                                                        >  7:141092/1‑68 (MQ=255)
                                                                   cTGAAGGTGTTGAAGCAGCAATTGCGCAGCAGGCGCAGCCAATCGTGACGCATCCCTGGCACGCCCgg                                                                                                                        <  8:141092/68‑1 (MQ=255)
                                                                    tGAAGGTGTTGAAGCAGCAATTGCGCAGCAGGCGCAGCCAATCGTGACGCATCCCTGGCACGCCCGGCACCTG‑CCGGGCATCGGGTGAAATCAGTTCACGGTTTCACCCTTGGGCTCAGCCGCCACATGCCcgcg                                                   <  1:295870/135‑1 (MQ=255)
                                                                       aGGTGTTGAAGCAGCAATTGCGCAGCAGGCGCAGCCAATCGTGACGCATCCCTGGCACGCCCGGCACCTG‑CCGGGCATCGGGTGAAATCAGTTCACGGTTTCACCCTTGGGCTCAGCCGCCACATGCCCGCGGc                                                 <  6:49917/134‑1 (MQ=255)
                                                                               aaGCAGCAATTGCGCAGCAGGCGCAGCCAATCGTGACGCATCCCTGGCACGCCCGGCACCTT‑CCGGGCATAGGGTGCAATCAGTTCACGGTTTCACCCATGGGCTCAGCCCCCACATGCCCCCCGCTcccccacc                                        >  2:212629/1‑135 (MQ=255)
                                                                                            gcagcaGGCGCAGCCAATCGTGACGCATCCCTGGCACGCCCGGCACCTG‑CCGGGCATCGGGTGAAATCAGTTCACGGTTTCACCCTTGGGCTCAGCCGCCACATGCCCGCGGCTCACCCACCAGCCAAACCCgg                            <  5:291157/134‑1 (MQ=255)
                                                                                             ccgcagGCGCAGCCATTCGTGAGGCATCCCGGGCACGCCCGGCACCTG‑CGGGGCATCGGGTGAAATCAGTTCACGGTTTCACCCTTGGGCTCAGCCGCCACATCCCCGCGGCTCACCCACCAGCCAAACCCGGcc                          <  5:173465/133‑1 (MQ=255)
                                                                                                cagGCGCAGCCAATCGTGACGCATCCCTGGCACGCCCGGCACCTG‑CCGGGCATCGGGTGAAATCAGTTCACGGTTTCACCCTTGGGCTCAGCCGCCACATGCCCGCGGCTCACCCACCAGCCAAACCCGGCcgcg                       <  2:69044/135‑1 (MQ=255)
                                                                                                     gcAGCCAATCGTGACGCATCCCTGGCACGCCCGGCACCTG‑CCGGGCATCGGGTGAAATCAGTTCACGGTTTCACCCTTGGGCTCAGCCGCCACATGCCCGCGGCTCACCCACCAGCCAAACCCGGCCGCGGTaaa                  <  5:130302/135‑1 (MQ=255)
                                                                                                     gcAGCCAATCGTGACGCATCCCTGGCACGCCCGGCACCTG‑CCGGGCATCGGGTGAAATCAGTTCACGGTTTCACCCTTGGGCTCAGCCGCCACATGCCCGCGGCTCACCCACCAGCCAAACCCGGCCGCGGTaaa                  <  1:183071/135‑1 (MQ=255)
                                                                                                       aGCCAATCGTGACGCATCCCTGGCACGCCCGGCACCTG‑CCGGGCATCGGGTGAAATCAGTTCACGGTTTCACCCTTGGGCTCAGCCGCCACATGCCCGCGGCTCACCCACCAGCCAAACCCGGCCGCGGTAAAGa                <  7:55617/135‑1 (MQ=255)
                                                                                                          cAATCGTGACGCATCCCTGGCACGCCCGGCACCTG‑CCGGGCATCGGGTGAAATCAGTTCACGGTTTCACCCTTGGGCTCAGCCGCCACATGCCCGCGGCTCACCCACCAGCCAAACCCGGCCGCGGTAAAGAACa             <  8:41594/135‑1 (MQ=255)
                                                                                                               gTGACGCATCCCTGGCACGCCCGGCACCTG‑CCGGGCATCGGGTGAAATCAGTTCACGGTTTCACCCTTGGGCTCAGCCGCCACATGCCCGCGGCTCACCCACCAGCCAAACCCGGCCGCGGTAAAGAACATGATc        <  1:13400/135‑1 (MQ=255)
                                                                                                                tGACGCATCCCTGGTACGCCCGGCACCTG‑CCGGGCATCGGGTGAAATCAGTTCACGGTTTCACCCTTGGGCTCCGCCGCCACATGCCCGCCGCTCACCCCCCCGCCAACCCCCGCCCCCGGTAAGACCAAGATCa       >  4:167993/1‑135 (MQ=255)
                                                                                                                tGACGCATCCCTGGCACGCCCGGCACCTG‑CCGGGCATCGGGTGAAATCAGTTCACGGTTTCACCCTTGGGCTCAGCCGCCACATGCCCGCGGCTCACCCACCAGCCAAACCCGGCCGCGGTAAAGAACATGATc        <  6:115231/134‑1 (MQ=255)
                                                                                                                tGACGCATCCCTGGCACGCCCGGCACCTG‑CCGGGCATCGGGTGAAATCAGTTCACGGTTTCACCCTTGGGCTCAGCCGCCACATGCCCGCGGCTCACCCACCAGCCAAACCCGGCCGCGGTAAAGAACATGATc        <  3:36241/134‑1 (MQ=255)
                                                                                                                      aTCCCTGGCACGCCCGGCACCTA‑CCGGGCATCGGGTGAAATCAGTTCACGGTTTCACCCTTGGGCTCAGCCGCCACATGCCCGCGGCTCACCCACCAGCCAAACCCGGCCGCGGGAAAGAACATGATCAATCTg  >  1:5237/1‑134 (MQ=255)
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CCGCTTGCACCATGCGGTGAAGGGGCGCACGGGGAGCGTGTCGTTCGGGTTCTTCTATGCGCCGCCGCTGAAGGTGTTGAAGCAGCAATTGCGCAGCAGGCGCAGCCAATCGTGACGCATCCCTGGCACGCCCGGCACCTG‑CCGGGCATCGGGTGAAATCAGTTCACGGTTTCACCCTTGGGCTCAGCCGCCACATGCCCGCGGCTCACCCACCAGCCAAACCCGGCCGCGGTAAAGAACATGATCAAGCTG  >  KT2440/3673657‑3673908

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑