Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,218,0680AC24.0% 220.1 / 18.2 121intergenic (‑553/+286)CJ019_03957/CJ019_03958hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (52/40);  new base C (0/29);  total (52/69)
Fisher's exact test for biased strand distribution p-value = 3.38e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 125 total aligned reads displayed.

CAGAAGACCTCGAAATTCACACTTGTTGAACGATGGAGATTCCGTATGACAGATCGTCGCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGGTA  >  KT2440/4217943‑4218202
                                                                                                                              |                                                                                                                                      
cAGAAGACCTCGAAATTCACACTTGTTGAACGATGGAGATTCCGTATGACAGATCGTCGCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCa                                                                                                                                <  8:29328/134‑1 (MQ=255)
 agaacaCCTCAAAATTAACAATTGTTGAACGATGGATATTCCGTCTGACATATCGTCGCCCTATCAATCCAAACTAAAATGGCCCTAAGCCTGGCGGCGGGCAACAACCTCGCTCCCTTAACAA‑AGACGGCCAGa                                                                                                                              <  8:263161/135‑1 (MQ=255)
  gaagaCCTCGAAATTCACACTTGTTGAACGATGGAGATTCCGTATGACAGATCGTCGCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGAt                                                                                                                             >  6:200876/1‑135 (MQ=255)
  gaagaCCTCGAAATTCACACTTGTTGAACGATGGAGATTCCGTATGACAGATCGTCGCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGAt                                                                                                                             >  8:253210/1‑135 (MQ=255)
  gaagaCCTCGAAATTCACACTTGTTGAACGATGGAGATTCCGTATGACAGATCGTCGCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGAt                                                                                                                             >  8:183738/1‑135 (MQ=255)
  gaagaCCTCGAAATTCACACTTGTTGAACGATGGAGATTCCGTATGACAGATCGTCGCCCTATCAATCAAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGAAACAA‑AGACGGCCAGAt                                                                                                                             >  7:257033/1‑135 (MQ=255)
         tctaaaTTCACAGTTGTTTATCGCTGGATATTCCGTATCACTGTTGTGCTCCCTATCAAACCAAACGTAAATGTCCCTACGGCGGGCGGCGGGGAACACCCTCGCTCCGGCACCAC‑CGACGGCCAGATACGTGAt                                                                                                                      <  6:206271/132‑1 (MQ=255)
         tCGAAATTCACACTTGTTGAACGATGGAGATTCCGTATGACAGATCGTCGCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGa                                                                                                                       >  1:178205/1‑134 (MQ=255)
         tCGAAATTCACACTTGTTGAACGATGGAGATTCCGTATGACAGATCGTCGCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGa                                                                                                                       >  8:189949/1‑134 (MQ=255)
         tCGAAATTCACACTTGTTGAACGATGGAGATTCCGTATGACAGATCGTCGCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGa                                                                                                                       >  2:178987/1‑134 (MQ=255)
           gAAATTCACACTTGTTGAACGATGGAGATTCCGTATGACAGATCGTCGCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATaa                                                                                                                    >  1:72650/1‑135 (MQ=255)
           gAAATTCACACTTGTTGAACGATGGAGATTCCGTATGACAGATCGTCGCCCTATAAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATaa                                                                                                                    >  4:33654/1‑135 (MQ=255)
            aaaTTCACACTTGTTGAACGATGGAGATTCCGTATGACAGATCGTCGCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAg                                                                                                                   >  4:152842/1‑135 (MQ=255)
              aTTCACACTTGTTGAACGATGGAGATTCCGTATGACAGCGCGGCGCCCTATCAATCCAAACGTAAATGGCCCTACGCCGGGCGGCGGGGAACAACCTCGCTCCCGAAACAA‑ACACGGCCAGATACGTGATAAGCg                                                                                                                 <  5:131417/135‑1 (MQ=255)
              aTTCACACTTGTTGAACGAGGGAGATGCCGTATGACAGAGCGTCGCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCGCCCGAAACAA‑AGACGGCCAGATACGTGATAAGCg                                                                                                                 <  3:125951/135‑1 (MQ=255)
              aTTCACAATTGTTTAACTATGGAGATTCCTTATGACAGATCGGCGCCCTATCAAACCAAACGTAATTGTCCCTAAGCCTGGCGGCGGGCAACAACCGCGCTCCCGAAACAA‑AGACGGCCAGATACGTGATAAGCg                                                                                                                 <  2:247637/135‑1 (MQ=255)
                            aaCGATGGAGATTCCGTATGACAGATCGTCGCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGa                                                                                                    >  1:192715/1‑134 (MQ=255)
                            aaCGATGGAGATTCCGTATGACAGATCGTCGCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGa                                                                                                    >  7:58444/1‑134 (MQ=255)
                                 tGGAGATTCCGTATGACAGATCGTCGCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAcccc                                                                                              >  2:101950/1‑135 (MQ=255)
                                  ggAGATTCCGTATGACAGAGCGTCGCCCTAACAATCCAAACGTAAATGGCCCTAAGCCTGGCGGCGGGGAACAACCTCGCCCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAAccc                                                                                              <  4:140465/134‑1 (MQ=255)
                                   gagaTTCCGTATGACAGATCGTCGCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCcg                                                                                            >  7:172447/1‑135 (MQ=255)
                                      aTTCCGTATGACAGATCGTCGCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCcgcg                                                                                          >  5:50896/1‑134 (MQ=255)
                                        tCCGTATCACAGTTCGTCCCCCAAACAATCCAAAGCAAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCACCCGTAACAA‑CCACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGaa                                                                                       <  2:72650/135‑1 (MQ=255)
                                           gTATGACAGATCGTCGCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGaaaa                                                                                     >  3:94410/1‑134 (MQ=255)
                                                 cAGCTCGGCGCCCAATCAAACCAAACGTAAATGCCCCACAGCCGGGCGGCGGGCAACAACCGCGCTCCCGAAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCt                                                                              <  1:219415/135‑1 (MQ=255)
                                                 cAGATCGTCGCCCTATCAATCCAAACGTAACTGTCCCTACGCCTGGCGGCGGGGAACAACCGCGCACCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCt                                                                              <  7:279465/135‑1 (MQ=255)
                                                 cAGATCGTCGCCATATCAATCCAAACGTAAATGTCCCTACGCCGGGCGGCGGGTAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCt                                                                              <  7:275911/135‑1 (MQ=255)
                                                      cgtcgCCCTATCAATCCAAACGTAATTGGCCCTACGCGGGGCGGCGGGTAACAACCTCCCGCCCTACACAA‑CCACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTg                                                                         <  8:127406/135‑1 (MQ=255)
                                                      cgtcgCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCTGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTg                                                                         <  8:130814/135‑1 (MQ=255)
                                                       gtcgCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGCGCGATGCGGGAAACCCCCGCGGAAAATAAACCTAGGTgg                                                                        >  4:18514/1‑135 (MQ=255)
                                                       gtcgCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTgg                                                                        >  8:143603/1‑135 (MQ=255)
                                                       gtcgCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTgg                                                                        >  1:216177/1‑135 (MQ=255)
                                                       gtcgCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTgg                                                                        >  3:138504/1‑135 (MQ=255)
                                                       gtcgCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTg                                                                         >  7:26178/1‑134 (MQ=255)
                                                       gtcgCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTg                                                                         <  8:26178/134‑1 (MQ=255)
                                                       gtcgCCATATCAAACCAAACTAAATTGACCCTAAGCCTGGCGGCGGGGAACAACCTCGCCCCCGAAACAA‑CCACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTgg                                                                        <  1:83695/135‑1 (MQ=255)
                                                         cgCCCTATCAATCCAACCTTAACTGTCCCTACGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGcc                                                                      <  3:84200/135‑1 (MQ=255)
                                                         cgCCCTATAAATCAAAAGGTAATTGTCCCTAATCCTGGCGGCGGGGAACAACCTCGCCCCCGTAAAAC‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAATCCTAGGTGGcc                                                                      <  4:72638/135‑1 (MQ=255)
                                                         cccccTATAAATCAAACCTTAAATGTCCCTAAGCCTGGCGGCGGGTAACAACCTCGCTCCCTAAACAA‑CGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGcc                                                                      <  5:117711/133‑1 (MQ=255)
                                                            ccaaTCAACCCAAACTTAAAGGGCCCTCCCCGTGGCGGCGGGCAACAACCTCCCCCCCCAAACAA‑CCACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCcag                                                                   <  5:242409/132‑1 (MQ=255)
                                                            ccTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTACCAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCcag                                                                   >  2:273631/1‑135 (MQ=255)
                                                            catATCAATCCAAAAGAAATTGTCACTAAGCCTGGCGGCGGGTAACAACGTCGCTCCCGTAACTA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCcag                                                                   <  2:44201/133‑1 (MQ=255)
                                                              tATCAATCCAACCGTAAATGACCCTAAGCCTGGCGGCGGGCAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCcagca                                                                 <  7:238630/135‑1 (MQ=255)
                                                              tATCAAACAAAACTAAATTCTCCCAACGCCGGGCGGGGGGTAAAAACCTCGCCCCCATAAAAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCcagca                                                                 <  6:135167/135‑1 (MQ=255)
                                                                 caaaccaaacGAAAATGGCCCTACGCCTGGCGGCGGGGAACAACCTCGCTCCCGAAACAC‑ACACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTg                                                              <  2:187671/135‑1 (MQ=255)
                                                                 cAATCCAACCCTACATGCCCATTCGCCGGGCGGCGGCAAACACCCTCCCCCCCCAACCAA‑CGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTg                                                              <  1:268561/135‑1 (MQ=255)
                                                                 cAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTg                                                              <  2:216177/135‑1 (MQ=255)
                                                                 cAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑ACACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTg                                                              <  8:93446/135‑1 (MQ=255)
                                                                 cAATCCAAACCTAAAGCTCCCTACCCCTGGCGGCGGGCACCACCCGCGCCCCCGTAACCA‑CGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTg                                                              <  2:168857/135‑1 (MQ=255)
                                                                   aTCCAAACTTAAATGTCCCTACGCCTGGCGGCGGGCAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCa                                                            <  4:292719/135‑1 (MQ=255)
                                                                   aTCCAAACGTAAATGTCCCTACGCCTGGCGGCGGGGAACAACCTCGCGCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCa                                                            <  8:5503/135‑1 (MQ=255)
                                                                      cAACCGTAAATGGCCCTACGCCGGGCGGCGGGGAAAAACCGCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCt                                                         <  4:274972/135‑1 (MQ=255)
                                                                           gTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCa                                                    >  7:282006/1‑135 (MQ=255)
                                                                           gTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCa                                                    >  8:37945/1‑135 (MQ=255)
                                                                           gTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCa                                                    >  1:69022/1‑135 (MQ=255)
                                                                            tacaTGCCCCAAACCCTGCCCGCGGGCAACACCCGCCCGCCCCTAACAA‑CCACGGCCACATACGTGAAAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATACACCTAGGGGGCCCAGCAGTGCACCTGTGCAt                                                   <  2:276570/132‑1 (MQ=255)
                                                                            tAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCAt                                                   >  2:184848/1‑135 (MQ=255)
                                                                                 gTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATAc                                                                                                                           <  7:48917/58‑1 (MQ=255)
                                                                                 gTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATAc                                                                                                                           >  8:48917/1‑58 (MQ=255)
                                                                                    cctccgCCTGGGGGCGGGCAACCACCCCCCCCCCCACACAA‑CCACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGtt                                           <  1:148254/130‑1 (MQ=255)
                                                                                    cctaccccTGGCGGCGGGGAACAACCGCGCTCCCGAAACAA‑CGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGtt                                           <  8:8425/129‑1 (MQ=255)
                                                                                    ccaacgCCGGGCGGCGGGCAACACCCTCGC‑CCCCCAACAACCCACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGtt                                           <  3:168633/130‑1 (MQ=255)
                                                                                    ccTACGCGTGGCGGCGGGCAACACCCGCGCGCCCTGAACAA‑CCACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGtt                                           <  7:289512/135‑1 (MQ=255)
                                                                                    ccTAAGCCTGGCGGCGGGGAACAACCTCGCCCCCGCACCAA‑AGACGGCCAGATACGTGATCAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGtt                                           <  1:114932/135‑1 (MQ=255)
                                                                                    catAAGCCGGGCGGTGGGCAAATACGTCGCTCAGCTAAAAA‑AGACGTCGAGATACGTGATAAGCGGAGCGATGCTGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCGAGCAGTGCACCTGTGCATTGCTGGtt                                           <  7:148271/133‑1 (MQ=255)
                                                                                     ctccgCCTGGCGGCGGGTAACAACCTCGCTCCCGTAACAA‑CGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTg                                          <  8:268861/131‑1 (MQ=255)
                                                                                     cTACGCCTGGCGGCGGTCAACAACCTCGCTCCCTTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTg                                          <  8:172447/135‑1 (MQ=255)
                                                                                       aaGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGcc                                        >  5:193744/1‑135 (MQ=255)
                                                                                       aaGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGcc                                        >  2:305464/1‑135 (MQ=255)
                                                                                          ccTGGCGGCGGCCAACAACCTCGCCCCCTAAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACg                                     <  8:282006/135‑1 (MQ=255)
                                                                                           cTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACgt                                    <  7:185396/135‑1 (MQ=255)
                                                                                               cggaggGGAACAACCTCGCTCCCGTAACAA‑AGACGCCCAGATACGTGATAAGCGGAGCGCTGCGGGAAAACCCCGCGGAAAATAAACCTAGCTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACgtgt                                  >  1:54625/1‑133 (MQ=255)
                                                                                                 gcggGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGTAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAg                              >  3:116214/1‑135 (MQ=255)
                                                                                                 gcggGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAg                              >  1:52538/1‑135 (MQ=255)
                                                                                                 gcggGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAg                              >  5:94625/1‑135 (MQ=255)
                                                                                                 gcggGGAACAACCTAGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAg                              >  3:101956/1‑135 (MQ=255)
                                                                                                  cggGTAACAACCTCGCTCCCGTAACAA‑ACACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAgg                             <  2:206133/135‑1 (MQ=255)
                                                                                                  cggGGAACAACCTCGCGCCCGTAAAAA‑CGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCTGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAgg                             <  6:54449/135‑1 (MQ=255)
                                                                                                  cggGCAACAACCTCGCTCCCGTAACAA‑CGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAgg                             <  8:219318/135‑1 (MQ=255)
                                                                                                    gggAACACCCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCt                           <  8:166607/135‑1 (MQ=255)
                                                                                                    gggAACAACCTCGCTCCCGAAACAA‑CCACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCt                           <  1:59975/135‑1 (MQ=255)
                                                                                                         cacactccctccCATAAAAA‑CCACGGCCAGATACGTGATAAGAGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGa                      <  5:176019/131‑1 (MQ=255)
                                                                                                         caacCTCGCTCCCTTAAAAC‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGa                      <  7:253210/135‑1 (MQ=255)
                                                                                                         caacCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGa                      <  8:88601/135‑1 (MQ=255)
                                                                                                         caacCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGa                      <  2:69022/135‑1 (MQ=255)
                                                                                                         caacCTCGCTCCCGAAAAAA‑AGACGTCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATATACCTAGGTGGCCGAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGa                      <  8:65877/135‑1 (MQ=255)
                                                                                                         caacCTCCCCCCCCTCACAC‑CCACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGa                      <  1:152130/135‑1 (MQ=255)
                                                                                                              gcGCTCCCGTAACAA‑CGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTaa                 <  7:174208/134‑1 (MQ=255)
                                                                                                                  tCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAg               >  4:111915/1‑133 (MQ=255)
                                                                                                                  tCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAg               >  3:16390/1‑133 (MQ=255)
                                                                                                                  tCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAg               >  2:9319/1‑133 (MQ=255)
                                                                                                                    ccGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACa            >  2:198123/1‑134 (MQ=255)
                                                                                                                    ccGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACa            >  4:184948/1‑134 (MQ=255)
                                                                                                                     ctaaacaa‑cGCCGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACa            <  1:210082/130‑1 (MQ=255)
                                                                                                                     ctaaaCAC‑ACACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTACGAGCTAAAGACAgg          <  5:282685/132‑1 (MQ=255)
                                                                                                                        aaCAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGCTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGGGTAAAGGCTTATGAGCTAAAGACAGGACt       >  2:66609/1‑135 (MQ=255)
                                                                                                                         aCAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTg      >  2:223177/1‑135 (MQ=255)
                                                                                                                         aCAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTg      >  2:64788/1‑135 (MQ=255)
                                                                                                                              aGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGGTa  <  8:205137/135‑1 (MQ=255)
                                                                                                                              aGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGGTa  >  4:108590/1‑135 (MQ=255)
                                                                                                                              |                                                                                                                                      
CAGAAGACCTCGAAATTCACACTTGTTGAACGATGGAGATTCCGTATGACAGATCGTCGCCCTATCAATCCAAACGTAAATGTCCCTAAGCCTGGCGGCGGGGAACAACCTCGCTCCCGTAACAA‑AGACGGCCAGATACGTGATAAGCGGAGCGATGCGGGAAAACCCCGCGGAAAATAAACCTAGGTGGCCCAGCAGTGCACCTGTGCATTGCTGGTTGCCACGTGTAAAGGCTTATGAGCTAAAGACAGGACTGGGTA  >  KT2440/4217943‑4218202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑