Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24404,666,7500AT23.8% 76.9 / 10.1 42intergenic (‑95/‑126)lasR/btuB_4Transcriptional activator protein LasR/Vitamin B12 transporter BtuB
Reads supporting (aligned to +/- strand):  ref base A (9/23);  new base T (10/0);  total (19/23)
Fisher's exact test for biased strand distribution p-value = 6.28e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGTGGTTTGTAAAGAACTTTCGTACAAGACCGTATGACGAAGGATGCGCACGATTTGCTGACTTTCTGCGTGCTCCAAGCCGCATTTCGCTCGTCTCAACGCCGTTGTCCATTCGCCTTTCGCCTGGAGTCGTACAATGCGTTCC  >  KT2440/4666621‑4666884
                                                                                                                                 |                                                                                                                                      
aGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGTGGTTTGTAAAGaa                                                                                                                                   <  6:275520/135‑1 (MQ=255)
aGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGTGGTTTGTAAAGaa                                                                                                                                   <  8:231076/135‑1 (MQ=255)
aGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGTGGTTTGTAAAGaa                                                                                                                                   <  4:296082/135‑1 (MQ=255)
aGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGTGGTTTGTAAAGaa                                                                                                                                   <  8:227191/135‑1 (MQ=255)
aGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGTGGTTTGTAAAGaa                                                                                                                                   <  6:83605/135‑1 (MQ=255)
        ctgcGAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGTGGGGTGTAAATAACTTTc                                                                                                                              >  1:50025/1‑132 (MQ=255)
        ctgcGAGTGTCGAGCGTTGTCAGGCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGTGGTTTGTAAAGAACTTTCGTa                                                                                                                           <  4:232403/135‑1 (MQ=255)
         tgcGTGTGTCGAGGGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCTCCGCCGTGGTTTGTAAAGAACTTTCGTa                                                                                                                           <  2:89596/134‑1 (MQ=255)
           cGAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGTGGTTTGTTAATAACATTCGTa                                                                                                                           >  4:69212/1‑132 (MQ=255)
            gAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGTGGTTTGTAAATAACTTTGGTACa                                                                                                                         <  8:230594/133‑1 (MQ=255)
            gAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGTGGGTTGTTAAGAACTTTCGTACaa                                                                                                                        >  6:281369/1‑134 (MQ=255)
                gtCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGTGGTTTGTAAAGAACTTTCGTACAAGAcc                                                                                                                    >  7:164142/1‑134 (MQ=255)
                gtCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGTGGGTTGTAAATAACAGTCGTTCAAGCCCg                                                                                                                   >  7:103238/1‑135 (MQ=255)
                     gCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGTGGTTTGTAAAGAACTTTCGTACAAGACCGTATGa                                                                                                              <  4:217090/135‑1 (MQ=255)
                        ttGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGTGGTTTGTAAAGAACTTTCGTACAAGACCGTATGACGa                                                                                                           <  2:246758/135‑1 (MQ=255)
                            cagtaaTGATTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGACGTGGTTTGTAAAGAACTTTCGTACAAGACCCTAAGACGAAg                                                                                                         >  2:172467/1‑133 (MQ=255)
                                caggtctGACCTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTTCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGTGGTTTGTAAACAACTTTCGTACAAGACCGTATGACGAAGGATgcg                                                                                                   <  7:153280/129‑1 (MQ=255)
                                aaTGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGTGGTTTGTAAAGAACTTTCGTACAAGACCGTATGACGAAGGATgcg                                                                                                   <  1:297618/134‑1 (MQ=255)
                                  tGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGTGGTTTGTAAAGAACTTTCGTACAAGACCGTATGACGAAGGATgcgc                                                                                                  <  8:98346/134‑1 (MQ=255)
                                        aaCTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGTGGGTTGTAAAGAACTTTAGTAAAAGACCCTATTGCGAAGGATGCGCACGCttt                                                                                           >  7:237950/1‑135 (MQ=255)
                                                gggTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGTGGGTTGTTAAGAACTTTAGTACAATCCCGTATGCCGAAGGATTCCCACGATTTGCTGACtt                                                                                   >  8:151375/1‑135 (MQ=255)
                                                       tGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGTGGGTTGTTAATAACTTTCGGACAAGACCGGATGTCGCAGGATGCGCCCGATTTTCTGGCTTTCTGCGt                                                                            >  1:160241/1‑135 (MQ=255)
                                                                      tgcCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGTGGTTTGTAAATAACTTTAGTACAAGACCCGATGACGAAGGCAGCGCACGATTTGCTGCCTTTCTGCGTGCTGCAACCCGCaat                                                             >  2:80869/1‑133 (MQ=255)
                                                                         cTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGTGGTTTGTAAAGAACTTTCGTACAAGACCGTATGACGAAGGATGCGCACGATTTGCTGACTTTCTGCGTGCTCCAAGCCGCATTTc                                                           <  8:196843/134‑1 (MQ=255)
                                                                           aCCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGTGGTTTGTTAAGAACTTTCGTACAAGACCGGATGACGAAGGATGCGGACGATTTGCTGGCTTTCTTCGTGCTCCACGCCGCATTTGGCt                                                        >  7:95900/1‑135 (MQ=255)
                                                                           aCCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGTGGTTTGTAAAGAACTTTCGTACAAGACCGTATGACGCAGGATGCGCACCATTTGCTGACTTTCTGCGTGCTGCAAGCCGCATGTCGCt                                                        >  5:105306/1‑135 (MQ=255)
                                                                           aCCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGTGGGTTGTTAAGAACATTAGTACAACACCGTATGACGAAGGCTGCGCACGATTTGCTGACTTTATGCGTGCTCCAAGCCGCCTTTCGCt                                                        >  6:104805/1‑135 (MQ=255)
                                                                           aCCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGTGGGTTGTAAAGAACTTTCGTACAAGACCGGATGACGAAGGATGCGCACGAGTTGCTGCCTTTCTGCGTGCTCCAAGCCGCCTTTCGCt                                                        >  8:14286/1‑135 (MQ=255)
                                                                                cAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGTGGTTTGTTTAGAAGATTAGTACAAGACCCTATGACGAAGGATGCGCACGATTTGCTGACATTATTCGTGCTGCAAGCCGCATTTGTCTCGtgt                                                   >  8:28072/1‑133 (MQ=255)
                                                                                  gTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGTGGGGTGTTAAGAACTTTCGTACAAGACCGGATTACGAAGGATGCGGCCGAATTGGTGTCTTTCTGGGGGGTCCAAGCCGCATTTAGCTCGTCTCa                                                 >  4:164978/1‑135 (MQ=255)
                                                                                  gTCACCTTACGAAGCACAGGCAACCTGCCTCCACCGCCGTGGTTTGTAAAGAACTTTCGTACAAGACCGTATGACGAAGGATGCGCACGATTTGCTGACTTTCTGCGTGCTCCAAGCCGCATTTCGCTCGTCTCa                                                 <  3:15064/135‑1 (MQ=255)
                                                                                             aaGCACAGGCCACCTGCCTCCACCGCCGTGGGTTGTTAAGAAAATTGCGAAAAGACAGTATGAAGAACGAATCGCACGCTTTGCAGCATATATTCGTGCTCCCAACCCCATTTAGCTCGTCACAACCCCCATGTc                                      >  4:212334/1‑135 (MQ=255)
                                                                                              aGCACAGGCCACCTGCCTCCACCGCCGTGGTTTGTAAAGAACTTTCGTACAAGACCGTATGACGAAGGATGCGCACGATTTGCTGACTTTCTGCGTGCTCCAAGCCGCATTTCGCTCGTCTCAACGCCGTTGTcc                                     <  5:7807/135‑1 (MQ=255)
                                                                                                   aGGCCACCTGCCTCCACCGCCGTGGTTTGTAAAGAACTTTCGTACAAGACCGTATGACGAAGGATGCGCACGATTTGCTGACTTTCTGCGTGCTCCAAGCCGCATTTCGCTCGTCTCAACGCCGTTGTCCAttcg                                <  2:148870/135‑1 (MQ=255)
                                                                                                         ccTGCCTCCACCGCCGTGGTTTGTAAAGAACTTTCGTACAAGACCGTATGACGAAGGATGCGCACGATTTGCTGACTTTCTGCGTGCTCCAAGCCGCATTTCGCTCGTCTCAACGCCGTTGTTCAttcgcctttc                          <  8:164142/135‑1 (MQ=255)
                                                                                                         ccTGCCTCCACCGCCGTGGTTTGTAAAGAACTTTCGTACAAGACCGTATGACGAAGGATGCGCACGATTTGCTGACTTTCTGCGTGCTCCAAGCCGCATTTCGCTCGTCTCAACGCCGTTGTCCAttcgcctttc                          <  5:281369/135‑1 (MQ=255)
                                                                                                         ccTGCCTCCACCGCCGTGGTTTGTAAAGAACTTTCGTACAAGACCGTATGACGAAGGATGCGCACGAGTTGCTGACTTTCTGCGTGCTCCAAGCCGCATTTCGCTCGTCTCAACGCCGTTGTCCAttcgcctttc                          <  6:287223/135‑1 (MQ=255)
                                                                                                              cTCCACCGCCGTGGTTTGTAAAGAACTTTCGTACAAGACCGTATGACGAAGGATGCGCACGATTTGCTGACTTTCTGCGTGCTCCAAGCCGCATTTCGCTCGTCTCAACGCCGTTGTCCAttcgcctttcgcct                      <  2:50025/134‑1 (MQ=255)
                                                                                                              cTCCACCGCCGTGGTTTGTAAAGAACTTTCGTACAAGACCGTATGACGAAGGATGCGCACGATTTGCTGACTTTCTGCGTGCTCCAAGCCGCATTTCGCTCGTCTCAACGCCGTTGTCCATTCGCCTTTCGCCTg                     <  3:211980/135‑1 (MQ=255)
                                                                                                                  aCCGCCGTGGTTTGTTAAGAACTTTCGTACAAGACCCGATGACGAAGGCTGGGCACGATTTTGTGACTTTCTGCGTGCTCCCAGCCGCCTTTAGCTCGTCTCAACGCCGGTGTCCATTCGCCTTTAGCCGGGGGt                 >  4:211980/1‑135 (MQ=255)
                                                                                                                  aCCGCCGTGGTTTGTAAAGAACTTTCGTACAAGACCGTATGACGAAGGATGCGCCCGATTTGCTGACTTTATTCGTGCTCCAAGCCGCAATTCGCTCGTCTCCACGCCGGTGTCCATTCGCCTTTCGCCTGGCGt                 >  8:150398/1‑135 (MQ=255)
                                                                                                                            tttGTAAAGAACTTTCGTACAAGACCGTATGACGAAGGATGCGCACGATTTGCTGACTTTCTGCGTGCTCCAAGCCGCATTTCGCTCGTCTCAACGCCGTTGTCCATTCGCCTTTCGCCTGGAGTCGTACAATGc       <  7:150398/135‑1 (MQ=255)
                                                                                                                                 aaaGAACTTTCGTACAAGACCGTATGACGAAGGATGCGCACGATTTGCTGACTTTCTGCGTGCTCCAAGCCGCATTTCGCTCGTCTCAACGCCGTTGTCCATTCGCCTTTCGCCTGGAGTCGTACAATGCGTTcc  <  5:104805/135‑1 (MQ=255)
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AGGGGTTGCTGCGAGTGTCGAGCGTTGTCAGTCATGTTTGAACTCCACGGGTGATTGAGCGGGGCATTGCTGCCTACCTGCAGTCACCTTACGAAGCACAGGCCACCTGCCTCCACCGCCGTGGTTTGTAAAGAACTTTCGTACAAGACCGTATGACGAAGGATGCGCACGATTTGCTGACTTTCTGCGTGCTCCAAGCCGCATTTCGCTCGTCTCAACGCCGTTGTCCATTCGCCTTTCGCCTGGAGTCGTACAATGCGTTCC  >  KT2440/4666621‑4666884

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑