| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 4,845,478 | 0 | T | G | 60.0% | ‑1.2 / 11.2 | 23 | intergenic (+47/+72) | apt/recR | Adenine phosphoribosyltransferase/Recombination protein RecR |
| Reads supporting (aligned to +/- strand): ref base T (1/7); new base G (8/4); total (11/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.81e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.89e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGAACTGGACGGGTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATTCGCGGGTAAACCCGCTCCTACGAAAGCCGCGTATGTTTTGTAGGCGCGGGT‑TTACCCGCG‑AA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCAC > KT2440/4845350‑4845606 | ggAACTGGACGGGTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATTCGCGGGTAAAACCGCACCTaa > 8:155690/1‑133 (MQ=255) tGGCCGGGTCGGGTCGTTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATTCGCGGGTAAACCCGCTCCTACGAAAGc < 3:18077/135‑1 (MQ=255) cgcgTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATTCGCGGGGTAACCCGCGCCGACGCAACCCGCGGATGt > 7:100730/1‑135 (MQ=255) gtggCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATTCGCGGGTAAACCCGCTCCTACGAAAGCCGCGTATGTTTTGAAg < 3:137886/132‑1 (MQ=255) ggCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATTCGCGGGTTAACCCCCACCCTCGAAATCCCCGGATTGGTTGTTGTCGCggggt > 8:61532/1‑133 (MQ=255) gggTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATTCGCGGGTTAAACCGCGCCGACGGAAGGCGCGGATGTTTTTTTTGGGCGGGG‑TTTccc > 6:106814/1‑135 (MQ=255) ctcccTTCTGCCAGACCGAGTTTTCCCTCCGCGAGGATGGCGTGGGCTTTTGGGGCTGCTTTGCAGCCCATTCGCGGGTAAACCCGCGCCTACTAACGCCGCGTATCTTTTGTAGCCGCGGGT‑TTACCCGCG‑AA‑t < 8:212579/132‑1 (MQ=25) cTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATTCGCGGGTTAAACCGCGCCAAAGAAACCCGCGGATTTTTTGTTGGCGCGGGT‑TTTACCGCG‑AATTGGgccc > 2:29208/1‑134 (MQ=255) tGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATTAGCGGGTTAAACCCCGCCGACGCAAGCAGCCGATGGTTTGTTTGGGCGGGG‑TTTCCCCCGAAA‑TGGgccc > 8:208670/1‑133 (MQ=255) tGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATTAGCCGGTAAAACCCCGCCTAACAAACACGCGGTTGGTTTTTAGGCGAGGGTGTTACCGCCC‑AA‑TGGCCCCCCaac > 2:195045/1‑134 (MQ=255) ggggCTTTTGGGGCTGCTTTGCAGCCCATTCGCGGGTAAACCCGCTCCTCCGAACCCCGCGTAGTTTTTGTCGGCGGGGGT‑TTACCCGCG‑AA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCg < 8:86143/134‑1 (MQ=37) gggCTTTTGGGGCTGCTTTGCAGCCCATTCGCGGGTAAACCCCCGGCGCGGCAAGCCCGGGATTGTTTGGTGGCGCGGGG‑TGTCCCGCG‑AA‑AGGGGCGCCCAGCGGGCCCCATTCATCCGGGCGGAAAGGGGCgg > 4:214213/1‑135 (MQ=255) gggggCTGCTTGCCAGCCCTTTCCCGGGAAACCCCGCGCCTCCGAAAGCCGGGTATGTTTGGTCGGCGCGGGT‑TTACCCGCG‑AA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCt < 1:177197/133‑1 (MQ=37) ggggCGGCTTGGCAGCCCATGCGCGGGAAAACCCGCGCCTAGCAACGCCGCGTATGTTTTGTAGGCGCGGGT‑TTACCCGCG‑AA‑TGGGCCGCCAAGCGGCCCCAATGAATTAGAGCGAAATGGGCCGGCGCCCTg < 4:174086/134‑1 (MQ=37) gggctTCGCAGCCCTTTCGCGGGTACACCCGCGACTACGAAAGCCGGGTATGTTTTGTCGGCGCGGTT‑TTACCCGCG‑AA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAg < 8:87237/133‑1 (MQ=255) tgctTTGCTGCCCATGCGCGGTTAACCCCGCTCCACCGAACCCCGCGTATGTTTGGTCGGGGGGGGT‑TTACCGGCG‑AA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAgg < 1:50697/135‑1 (MQ=255) ctTTGCAGCCCATTCGCGGGTTAACCCGCGCCTACGAAAGCCGCGGATGTTTTGGAGGCGGGGGG‑TTTCCCGCG‑GA‑TGGGGCGGCCAGCGGGCCCCATGGCTCCGCGGGGAATGGGCCGGCGCCCGGCCGAGGcc > 6:239949/1‑134 (MQ=255) ttGCAGCCCATTCGCGGGTAAACCCGCGCCGGCCGAAGCCGGGGATGTGTTGTTGGCGCGGGT‑TTTCCCGCG‑CA‑TTGGGCCCCCACCGGGCCCCATGAATCAGGGGGAAATGGGCCGGCGCCCGGCCAAGGCagg > 2:177197/1‑133 (MQ=255) gccgCCCATGCGCGGGTACACCCGCTCAACCCAACGCCGCGTCTGTTTTGTCGGCGGGGGT‑TTACCCGCT‑AA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATg < 3:214213/130‑1 (MQ=255) ccttgcgcgGGTACACCCGCTCCAACGAACGCCGCGTTGGTGTGGTAGGCGGGGGT‑TTACCCGCG‑AA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAgg < 3:52499/130‑1 (MQ=38) cgGGAAACCCCGCTCCACCCAACGCCGCGTAGGTTTTGTCGCCGCGGGT‑TAACCCGCG‑AA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTAccgc < 5:106814/135‑1 (MQ=255) cgGGAAACCCCGCCCCTACTACTCCCCCTTCTGTTTTGTCGGC‑CGGGTGTTCCCCGCG‑AA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTAccgc < 2:182434/135‑1 (MQ=255) aCCCGCTCCGTCGGAAGCCGCGGATGTTTTGTAGGCGCGGGT‑TTTCCCGCG‑CA‑TGGGCCCCCCAGCGGCCCCCATGGATGAGAGCGCAATGGGCCGGCGCCCGGCAAAGGCATGGGGCCGGGTTCCGCCGGcccc > 4:142826/1‑133 (MQ=255) | GGAACTGGACGGGTCGCGTCGGTTGCAAGCGGCCGGGGTGCCTACCTTCTGCCTGACCGAGTTTTCCCTCAGCGAGTATTGAGTGGGCTTTTGGGGCTGCTTTGCAGCCCATTCGCGGGTAAACCCGCTCCTACGAAAGCCGCGTATGTTTTGTAGGCGCGGGT‑TTACCCGCG‑AA‑TGGGCCGCCAAGCGGCCCCAATGAATCAGAGCGAAATGGGCCGGCGCCCTGCAAAGGCATGGGCCAGGGTACCGCCGTCCAC > KT2440/4845350‑4845606 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |