| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 4,884,498 | 0 | T | G | 53.3% | 5.8 / 12.6 | 15 | intergenic (+75/‑164) | glxR/ttuD | 2‑hydroxy‑3‑oxopropionate reductase/Putative hydroxypyruvate reductase |
| Reads supporting (aligned to +/- strand): ref base T (4/3); new base G (3/5); total (7/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.19e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.22e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCGCTGATCAAAGGCCTGGAGCACATGGCCAACTTCTCGATCCGCGACGACAAATAAATCGTTTTTAGCCTCACTGGCCTCTTCGCGGGTAAACCCGCTCCTACCAAAGACCGCACAATCGCTCAGAGCTGTGCATGTAGGAGCGGGCTTGCCCGCGAAGAAGCCAACGCCGTTGAACCTGCGACCACAGGCCCAATGGTGGCATCGAGCAACACCCGCCCCTGGTTCGGCCTGCACGGAGGCAGTTCCAGGGGCGTTTTTGAT > KT2440/4884366‑4884630 | ggCGCTGATCAAAGGCCTGGAGCACATGGCCAACTTCTCGATCCGCGACGACAAATAAATCGTTTTTAGCCTCACTGGCCTCTTCGCGGGTAAACCCGCTCCTACCAAAGACCGCACAATCGCTCAGAGCTGTGc > 3:238483/1‑135 (MQ=255) aaaGGCCTGGAGCACATGGCCAACTTCTCGATCCGCGACGACAAATAAATCGTTTTTAGCCTCACTGGCCTCTTCGCGGGTAAAACCGCGCCGACCAAAGCCCGCCCAATCGCTCAGAGCTGGGCATGTAGGGGc > 4:94491/1‑135 (MQ=255) aGGCCTGGAGCACATGGCCAACTTCTCGATCCGCGACGACAAATAAATCGTTTTTAGCCTCACTGGCCTCTTCGCGGGTAAACCCGCTCCTACCAAAGACCGCACAATCGCTCAGAGCTGTGCATGTAGGAGCgg < 4:238483/135‑1 (MQ=255) ggCCAACTTCTCGATCCGCGACGACAAATAAATCGTTTTTAGCCTCACTGGCCTCTTAGCGGGTAAACCCGCCCCAACCAAACACCCCACAAAACCGCACAGCTGGGCCTGTATGTGCGGGCTTGCCCGCgaaaa > 2:32625/1‑133 (MQ=255) ttCGTTTTTGGCCTCTGTGCCGTCTTCGCGGGTAAACCCGGACATACAAACGCCGGAAAAATCGCTCAGAGCTGTGATTGTCGGCGGGGGATTGCGCGCTATGAAGCCAACGCCGTTGAACCTGCGACCACAgg < 4:52000/133‑1 (MQ=255) gccccccTGGCCTATGCGCGGGAAACCCCGCCCCACCCAACGACCCCCCAATCCCTCAGCGCGGGGAATGTCGGCGGGGGCTGGCCGGCGAAGAAGCCAACGCCGTTGAACCTGCGACCACAGGCCCAAtggtgg < 2:63551/129‑1 (MQ=255) acgggCCTCTGCGGGGGAAACCCCGCGCCAACCAACCCCCCCACAAGCGCGCCGCGCGGGGCCTGTCGGCGGGGGCTGCCCCGCCAAGAAGCCAACGCCGTTGAACCTGCGACCACAGGCCCAATGGTGGCATCg < 4:140876/132‑1 (MQ=255) cgctgcgcgGGTAACCCCGCTCCAACCAAAGACCGCACAATCGCTCAGAGCTGGGCATGTAGGCGCGGGCTTGCCCGCGAAGAAGCCAACGCCGTTGAACCTGCGACCACAGGCCCAATGGTGGCATCGAGCAac < 3:94491/130‑1 (MQ=255) gcgGGTAAACCCGCACCTACCAAAGACCGCACAATCGCTCCGAGCTGGGCCTGTAGGAGCGGGCTTGCCCCCGAAGAAGCCCACCCCGTTTAACCTGCGACCACAGGCCCCATGGTGGCATACAGCAACACCCGc > 8:222143/1‑135 (MQ=255) cccGCACATACAAACGCCCGCACAATCGCGCTGAGCTCGGCTTGTCGGCGGGGGCTTGCCGGCGAAGAAGCCAACGCCGTTGAACCTGCGACCACAGGCCCAATGGTGGCATCGAGCAACACCCGCCCCTGGTTc < 6:304698/135‑1 (MQ=255) ccGCTCCAACAAAACACCGCACAAGCGCTCAGAGCGGTGCATGTAGGCGCGGGGTTGCCCGCGAAGAAGCCAACGCCGGTGAACCTGCGACCACAGGCCCAATGGTGGCATCGATCAACACCCGCCCCTGGTTCg < 7:255031/135‑1 (MQ=255) ccTACCAAAGACCGCACAATCGCTCAGAGCTGTGCATGTAGGAGCGGGCTTGCCCGCGAAGAAGCCAACGCCGTTGAACCTGCGACCACAGGCCCAATGGTGGCATCGAGCAACACCCGCCCCTGGTTCGGCCt > 2:255307/1‑134 (MQ=255) cAAACACCCCACAACCGCTCACAGCTGGCCAGGTAGGAGCGGGCTTCCCCGCGAAGAAGCCAACGCCGTTGAACCTGCGACCACAGGCCCAATGGTGGCATCGAGCAACACCCGCCCCTGGTTCGGCCTGCACgg < 3:28120/135‑1 (MQ=255) aaaGACCGCACAATCGCTCAGAGCTGTGCATGTAGGAGCGGGCTTGCCCGCGAAGAAGCCAACGCCGTTGAACCTGCGACCACAGGCCCAATGGTGGCATCGAGCCACACCCGCCCCTGGTTCGGCCTGCACGGa > 8:144623/1‑135 (MQ=255) aaTCGCTCAGAGCTGTGCATGTAGGAGCGGGCTTGCCCGCGAAGAAGCCAACGCCGTTGAACCTGCGACCACAGGCCCAATGGTGGCATCGCGCAACACCCGCCCCTGGTTCGGCCTGCACGGAGGCAGTTCCAg > 3:22372/1‑135 (MQ=255) tgCATGTAGGAGCGGGCTTGCCCGCGAAGAAGCCAACGCCGTTGAACCTGCGACCACAGGCCCAATGGTGGCATCGAGCAACACCCGCCCCTGGTTCGGCCTGCACGGAGGCAGTTCCAGGGGCGtgtttttt > 5:12442/1‑130 (MQ=255) | GGCGCTGATCAAAGGCCTGGAGCACATGGCCAACTTCTCGATCCGCGACGACAAATAAATCGTTTTTAGCCTCACTGGCCTCTTCGCGGGTAAACCCGCTCCTACCAAAGACCGCACAATCGCTCAGAGCTGTGCATGTAGGAGCGGGCTTGCCCGCGAAGAAGCCAACGCCGTTGAACCTGCGACCACAGGCCCAATGGTGGCATCGAGCAACACCCGCCCCTGGTTCGGCCTGCACGGAGGCAGTTCCAGGGGCGTTTTTGAT > KT2440/4884366‑4884630 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |