Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	KT2440	515,063	G→A	D236D (GAC→GAT)	mepM ←	Murein DD‑endopeptidase MepM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	515,063	0	G	A	100.0%	218.5 / NA	59	D236D (GAC→GAT)	mepM	Murein DD‑endopeptidase MepM
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (28/31);  total (28/31)

AGGTTTTGCCGCGGTTGGTGAAGCGCGCAGAGAGGATGTTACCGGTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATGTCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCACCCCGTGGGCATAGGCGGTGCGTATCACCGGCTTGCTGATT  >  KT2440/514933‑515192                                                                                                                                   |                                                                                                                                  aGGTTTTGCCGCGGTTGGTGAAGCGCGCAGAGAGGATGTTACCGGTGCCGACGACCTTGCCATCCATCCCCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCg                                                                                                                                >  1:254122/1‑134 (MQ=255)        gCCGCGGTTGGTGAAGCGCGCAGAGAGGATGTTACCGGTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCa                                                                                                                         >  7:170329/1‑134 (MQ=255)          cgcgGTTGGTGAAGCGCGCAGAGAGGATGTTACCGGTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGc                                                                                                                       <  5:90213/134‑1 (MQ=255)          cgcgGTTGGTGAAGCGCGCAGAGAGGATGTTACCGGTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGc                                                                                                                       <  4:237465/134‑1 (MQ=255)             ggTTGGTGAAGCGCGCAGAGAGGATGTTACCGGTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCAcgc                                                                                                                   >  8:156321/1‑135 (MQ=255)              gTTGGTGAAGCGCGCAGAGAGGATGTTACCGGTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCAcgcg                                                                                                                  <  2:121841/135‑1 (MQ=255)               tttgTGAAGCGCGCAGAGAGGATGTTACCGGTGCCTACGAACTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCgg                                                                                                                 <  6:290013/132‑1 (MQ=255)               ttGGTGAAGCGCGCAGAGAGGATGTTACCGGTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCgg                                                                                                                 <  7:207620/135‑1 (MQ=255)                    gAAGCGCGCAGAGAGGATGTTACCGGTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCAt                                                                                                            <  1:26879/135‑1 (MQ=255)                         gcgcAGAGAGGATGTTACCGGTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCa                                                                                                       <  8:125643/135‑1 (MQ=255)                            cAGAGAGGATGTTACCGGTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATgg                                                                                                    <  8:170329/135‑1 (MQ=255)                            cAGAGAGGATGTTACCGGTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATgg                                                                                                    >  3:271606/1‑135 (MQ=255)                                 agGATGTTACCGGTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATg                                                                                               <  2:253774/135‑1 (MQ=255)                                  ggATGTTACCGGTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCTTAACCCAGCACGCGGGCCATGTCCATGGTCATg                                                                                               <  7:131715/134‑1 (MQ=255)                                    aTGTTACCGGTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCtg                                                                                            >  4:165819/1‑135 (MQ=255)                                        tACCGGTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTggg                                                                                        >  6:275452/1‑135 (MQ=255)                                          ccGGTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTggg                                                                                        >  2:211757/1‑133 (MQ=255)                                                ccGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCCTGTCCATGGTCATGCTGTGGGACAGGCCg                                                                                >  3:176541/1‑135 (MQ=255)                                                ccGACGACCTAGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAAAATCCTCTGCGAAGTCGATATCGTAACCCAGCACGCGGGCAATGTCCATGGTCATGCTGTGGGACAgcacg                                                                                >  7:176168/1‑131 (MQ=255)                                                 cgacgaCCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCCCGCGGGCCATGTCCATGGTCCTGCTGTGGGACAGGCCgg                                                                               >  2:248646/1‑135 (MQ=255)                                                            ccatccatCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCg                                                                    >  6:71013/1‑135 (MQ=255)                                                             catccatcACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCCGCACGCGGGCCATGTCCATGGTCATGCtgtg                                                                                          >  2:214114/1‑112 (MQ=255)                                                             catccatcACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCtgtg                                                                                          <  1:214114/112‑1 (MQ=255)                                                                    cACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAg                                                             >  5:48476/1‑134 (MQ=255)                                                                      ccTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACa                                                          <  3:231655/135‑1 (MQ=255)                                                                      ccTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACa                                                          <  5:99941/135‑1 (MQ=255)                                                                      ccTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACa                                                          <  7:5846/135‑1 (MQ=255)                                                                             ttCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGc                                                                                                                       >  2:83678/1‑67 (MQ=255)                                                                             ttCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGc                                                                                                                       <  1:83678/67‑1 (MQ=255)                                                                                gTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGctct                                                 >  5:115272/1‑134 (MQ=255)                                                                                 tAGACCACGTCTAACTAGTCCCCAGTGCGAATATCCTGTGCTAATTCGTTATCGTAACCAAGCACGCGGGCCATTTCCATGTTCATTCTGTGGGACAGGCCGGCGCGCTGGGCGTAAGCAGACATCGAGCTCtt                                                <  2:221972/134‑1 (MQ=255)                                                                                      cACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCAc                                          <  8:117742/135‑1 (MQ=255)                                                                                       aCGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAATTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCAcc                                         <  1:211757/135‑1 (MQ=255)                                                                                       aCGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCAcc                                         <  7:194603/135‑1 (MQ=255)                                                                                       aCGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCAcc                                         <  5:134565/135‑1 (MQ=255)                                                                                       aCGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCAcc                                         <  3:165819/135‑1 (MQ=255)                                                                                       aCGTCGAACTCGGCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCAcc                                         <  5:88379/135‑1 (MQ=255)                                                                                                   tCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCACCCCGTGGGCAt                               <  8:216867/133‑1 (MQ=255)                                                                                                   tCCCCAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCACCCCGTGGGCATAg                             <  7:156321/135‑1 (MQ=255)                                                                                                   gccccAGGGCGAATATCCTGAGCGAAGTCGATATCGTAACCCAGCCCGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCACCCCGTGGGCATAg                             <  3:190976/134‑1 (MQ=255)                                                                                                      ccAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCtgtg                                                                                          >  5:244478/1‑71 (MQ=255)                                                                                                      ccAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCtgtg                                                                                          <  6:244478/71‑1 (MQ=255)                                                                                                      ccAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCACCCCGTGGGCATAGGCg                          >  4:188261/1‑135 (MQ=255)                                                                                                      ccAGGGCGAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCACCCCGTGGGCATAGGCg                          <  3:188261/135‑1 (MQ=255)                                                                                                             gAATATCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGCCCGGCGCGCTGGGCGGAAGCAGCCAGCGAGCTCTTGAGCCCCCCGTGGGCATAGCCGGGGCGTa                   >  4:143462/1‑135 (MQ=255)                                                                                                                  tCCTGTGCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCACCCCGTGGGCATAGGCGGTGCGTATCAcc              >  7:158029/1‑135 (MQ=255)                                                                                                                        gCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCtt                                                >  2:294175/1‑95 (MQ=255)                                                                                                                        gCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCtt                                                <  1:294175/95‑1 (MQ=255)                                                                                                                        gCGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCACCCCGTGGGCATAGGCGGTGCGTATCACCGGCTTg        >  8:118276/1‑135 (MQ=255)                                                                                                                         cGAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCACCCCGTGGGCATAGGCGGTGCGTATCACCGGCTTGc       >  1:121921/1‑135 (MQ=255)                                                                                                                          gAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGTGCTCTTGATCACCCCGTGGGCATAGGCGGTGCGTATCACCGGCTTGc       >  4:101190/1‑134 (MQ=255)                                                                                                                          gAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCAcccc                                       <  7:186454/102‑1 (MQ=255)                                                                                                                          gAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCAcccc                                       >  8:186454/1‑102 (MQ=255)                                                                                                                          gAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCACCCCGTGGGCATAGGCGGTGCGTATCACCGGCTTGc       >  6:134840/1‑134 (MQ=255)                                                                                                                          gAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCACCCCGTGGGCATAGGCGGTGCGTATCACCGGCTTGc       >  3:66532/1‑134 (MQ=255)                                                                                                                          gAAGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCACCCCGTGGGCATAGGCGGTGCGTATCACCGGCTTGc       >  3:150699/1‑134 (MQ=255)                                                                                                                            aGTCGATATCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCACCCCGTGGGCATAGGCGGTGCGTATCACCGGCTTGCTGa    >  3:262698/1‑135 (MQ=255)                                                                                                                              tCGATATCGTTACCCAGGACGCGGGCCATGTCCATGGTCATGCTGGTGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCACCCCGTGGGCATAGGCGGTGCGTATCACCGGCTTGCTGAtt  <  1:163864/135‑1 (MQ=255)                                                                                                                              tCGATATCGTAACCCAGCACGCGGGCGATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCACCCCGTGGGCATAGGCGGTGCGTATCACCGGCTTGCTGAtt  <  1:248646/135‑1 (MQ=255)                                                                                                                                   |                                                                                                                                  AGGTTTTGCCGCGGTTGGTGAAGCGCGCAGAGAGGATGTTACCGGTGCCGACGACCTTGCCATCCATCACCTTCTGTTCGTAGACCACGTCGAACTCGTCCCCAGGGCGAATATCCTGTGCGAAGTCGATGTCGTAACCCAGCACGCGGGCCATGTCCATGGTCATGCTGTGGGACAGGCCGGCGCGCTGGGCGGAAGCAGACAGCGAGCTCTTGATCACCCCGTGGGCATAGGCGGTGCGTATCACCGGCTTGCTGATT  >  KT2440/514933‑515192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑