Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	KT2440	3,502,855	C→T	P476S (CCG→TCG)	vgrG1_1 →	Actin cross‑linking toxin VgrG1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	3,502,855	0	C	T	100.0%	98.9 / NA	34	P476S (CCG→TCG)	vgrG1_1	Actin cross‑linking toxin VgrG1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (21/13);  total (21/13)

GTGGCTTCGGCAGCATCCAGATTCCGCGCGTAGGCGACGAAGTGGTGGTGGTGTTCCTGGACGGCAACCCGGACCGGCCGCTGATCATGGGCAGCCTGTACAACAGCACCAACACCCCGCCATGGTCGCTGCCGGCGAACAAGACCCAGAGTGGGTTCCTGACGCGGTCGATGAAGGGCGACGGCGGTACCGCCAACTTCTTCCGCTTCGAGGACAAGGCCGGCGCCGAGCAGATCATCATGCATGCCGAGCGCAACATGG  >  KT2440/3502724‑3502984                                                                                                                                    |                                                                                                                                  gTGGCTTCGGCAGCATCCAGATTCCGCGCGTAGGCGACGAAGTGGTGGTGGTGTTCCTGGACGGCAACCCGGACCGGCCGCTGATCATGGGCAGCCTGTACAACAGCACCAACACCCCGCCATGGTCGCTGTCgg                                                                                                                                >  1:191386/1‑135 (MQ=255) gTGGCTTCGGCAGCATCCAGATTCCGCGCGTAGGCGACGAAGTGGTGGTGGTGTTCCTGGACGGCAACCCGGACCGGCCGCTGATCATGGGCAGCCTGTACAACAGCACCAACACCCCGCCATGGTCGCTGTCgg                                                                                                                                >  6:24859/1‑135 (MQ=255)      ttCGGCAGCATCCAGATTCCGCGCGTAGGCGACGAAGTGGTGGTGGTGTTCCTGGACGGCAACCCGGACCGGCCGCTGATCATGGGCAGCCTGTACAACAGCACCAACACCCCGCCATGGTCGCTGTCGGCGaa                                                                                                                            >  8:7046/1‑134 (MQ=255)            agcaTCCAGATTCCGCGCGTAGGCGACGAAGTGGTGGTGGTGTTCCTGGACGGCAACCCGGACCGGCCGCTGATCATGGGCAGCCTGTACAACAGCACCAACACCCCGCCATGGTCGCTGTCGGCGAACAAGAc                                                                                                                      >  6:123370/1‑134 (MQ=255)                tCCAGATTCCGCGCGTAGGCGACGAAGTGGTGGTGGTGTTCCTGGACGGCAACCCGGACCGGCCGCTGATCATGGGCAGCCTGTACAACAGCACCAACACCCCGCCATGGTCGCTGTCGGCGAACAAGACCCaga                                                                                                                 <  5:114979/135‑1 (MQ=255)                 ccAGATTCCGCGCGTAGGCGACGAAGTGGTGGTGGTGTTCCTGGACGGCAACCCGGACCGGCCGCTGATCATGGGCAGCCTGTACAACAGCACCAACACCCCGCCATGGTCGCTGTCGGCGAACAAGACCCagag                                                                                                                >  7:101664/1‑135 (MQ=255)                   aGATTCCGCGCGTAGGCGACGAAGTGGTGGTGGTGTTCCTGGACGGCACACCGGACCGGCCGCTGATCATGCGCCGCCTGTACAACAGCACCAACACCCCGCCAGCGTCGCTGTCGGCGAACAAGCCCCAGAGTg                                                                                                              >  4:13167/1‑135 (MQ=255)                                          gtggtggtgttgGGCGTGGACGCCAACCCGGACCGGCCGCTGATCATGGGCAGCCTGTACAACAGCACCAACACCCCGCCATGGTCGCTGTCGGCGAACAAGACCCAGAGTGGGTTCCTGCCGCGGTCGATGAAg                                                                                       <  8:231061/135‑1 (MQ=255)                                          gtggtggtggtgTTCCTGGACGGCAACCCGGACCGGCCGCTGATCATGGGCAGCCTGTACAACAGCACCAACACCCCGCCATGGTCGCTGTCGGCGAACAAGACCCAGAGTGGGTTCCTGACGCGGTCGATGAAg                                                                                       <  7:7046/135‑1 (MQ=255)                                                   gtgtTCCTGGACGGCAACCCGGACCGGCCGCTGATCATGGGCAGCCTGTACAACAGCACCAACACCCCGCCATGGTCGCTGTCGGCGAACAAGAc                                                                                                                      >  6:229027/1‑95 (MQ=255)                                                   gtgtTCCTGGACGGCAACCCGGACCGGCCGCTGATCATGGGCAGCCTGTACAACAGCACCAACACCCCGCCATGGTCGCTGTCGGCGAACAAGAc                                                                                                                      <  5:229027/95‑1 (MQ=255)                                                           ggACGGCAACCCGGACCGGCCGCTGATCATGGGCAGCCTGTACAACAGCACCAACACCCCGCCATGGTCGCTGTCGGCGAACAAGACCCAGAGTGGGTTCCTGACGCGGTCGATGAAGGGCGACGGCGGTACCGc                                                                      >  3:79394/1‑135 (MQ=255)                                                             aCGGCAACCCGGACCGGCCGCTGATCATGGGCAGCCTGTACAACAGCACCAACACCCCGCCATGGTCGCTGTCGGCGAACAAGACCCAGAGTGGGTTCCTGACGCGGTCGATGAAGGGCGACGGCGGTACCGccc                                                                    >  4:239042/1‑134 (MQ=255)                                                              cGGCAACCCGGACCGGCCGCTGATCATGGGCAGCCTGTACAACAGCACCAACACCCCGCCATGGTCGCTGTCGGCGAACAAGACCCAGAGTGGGTTCCTGACGCGGTCGATGAAGGGCGCCGGCGGTACccccca                                                                   >  5:236527/1‑133 (MQ=255)                                                                        gACCGGCCGCTGATCATGGGCAGCCTGTACAACAGCACCAACACCCCGCCATGGTCGCTGTCGGCGAACAAGACCCAGAGTGGGTTCCTGAGGCGGTCGATGAAGGGCGACGGCGGTACCGCCAACTTCTTCCGc                                                         <  6:102882/135‑1 (MQ=255)                                                                         aCCGGCCGCTGATCATGGGGAGCCTGTACAACAGCACCAACACCCCGCGATGGTCGGTGTCGGCTAACAAGACCCAGAGTGGGTTCCTGACGCGGTCGATGAAGGGCGACGGCGGTACCGCCAACTTCTTCCGCt                                                        <  8:75979/135‑1 (MQ=255)                                                                              ccGCTGATCATGGGCAGCCTGTACAACAGCACCAACACCCCGCCATGGTCGCTGTCGGCGAACAAGACCCAGAGTGGGTTCCTGACGCGGTCGATGAAGGGCGACGGCGGTACCGCCAACTTCTTCCGCTTCGAg                                                   <  4:79394/135‑1 (MQ=255)                                                                              ccGCTGATCATGGGCAGCCTGTACAACAGCACCAACACCCCGCCATGGGCGCTGTCGGCGAACAAGACCCAGAGTGGGTTCCTGACGCGGTCGATGAAGGGCGACGGCGGTACCGCCAACTTCTTCCGCTTCGAg                                                   <  8:105741/135‑1 (MQ=255)                                                                                     tCATGGGCAGCCTGTACAACAGCACCAACACCCCGCCATGGTCGCTGTCGGCGAACAAGACCCAGAGTGGGTTCCTGACGCGGTCGATGAAGGGCGACGGCGGTACCGCCAACTTCTTCCGCTTCGAGGACaa                                              <  7:202881/133‑1 (MQ=255)                                                                                        tGGGCAGCCTGTACAACAGCACCAACACCCCGCCATGGTCGCTGTCGGCGATCAAGACCCAGAGTGGGTTCCTGACGCGGTCGATGAAGGGCGACGGCGGTACCGCCAACTTCTTCCGCTTCGAGGACAAGGCCg                                         <  4:230244/135‑1 (MQ=255)                                                                                                      aacaGCACCAACACCCCGCCATGGTCGCTGTCGGCGAACAAGACCCAGAGTGGGTTCCTGACGCGGTCGATGAAGGGCGACGGCGGTACCGCCAACTTCTTCCGCTTCGAGGACAAGGCCGGCGCCGAGCAGat                            >  8:227100/1‑134 (MQ=255)                                                                                                        caGCACCAACACCCCGCCATGGTCGCTGTCGGCGAACAAGACCCAGAGTGGGTTCCTGACGCGGTCGATGAAGGGCGACGGCGGTACCGCCAACTTCTTCCGCTTCGAGGACAAGGCCGGCGCCGAGCAGatca                          >  6:69649/1‑134 (MQ=255)                                                                                                            aCCAACACCCCGCCATGGTCGCTGTCGGCGAACAAGACCCAGAGTGGGTTCCTGACGCGGTCGATGAAGGGCGACGGCGGTACCGCCAACTTCTTCCGCTTCGAGGACAAGGCCGGCGCCGAGCAGATcatcat                      >  7:34986/1‑134 (MQ=255)                                                                                                              cAACACCCCGCCATGGTCGCTGTCGGCGAACAAGACCCAGAGTGGGTTCCTGACGCGGTCGATGAAGGGCGACGGCGGTACCGCCAACTTCTTCCGCTTCGAGGACAAGGCCGGCGcc                                    >  6:157057/1‑118 (MQ=255)                                                                                                              cAACACCCCGCCATGGTCGCTGTCGGCGAACAAGACCCAGAGTGGGTTCCTGACGCGGTCGATGAAGGGCGACGGCGGTACCGCCAACTTCTTCCGCTTCGAGGACAAGGCCGGCGcc                                    <  5:157057/118‑1 (MQ=255)                                                                                                              cAACACCCCGCCATGGTCGCTGTCGGCGAACAAGACCCAGAGTGGGTTCCTGACGCGGTCGATGAAGGGCGACGGCGGTACCGCCAACTTCTTCCGCTTCGAGGACAAGGCCGGCGCCGAGCAGATCATcatgca                   >  8:234789/1‑135 (MQ=255)                                                                                                              cAACACCCCGCCATGGTCGCTGTCGGCGAACAAGACCCAGAGTGGGTTCCTGACGCGGTCGATGAAGGGCGACGGCGGTACCGCCAACTTCTTCCGCTTCGAGGACAAGGCCGGCGCCGAGCAGATCATcatgca                   >  7:45926/1‑135 (MQ=255)                                                                                                                   ccccGCCATGGTCGCTGTCGGCGAACAATACCCAGAGTGGGATCCAGACGCCCTCCATGAAGGGCCACGGCGGTACCGCCAACTACATCCGAATCTACGACAACGCCGGCGCCGAGCAGATCATCatgcatgc                >  5:100055/1‑133 (MQ=255)                                                                                                                   ccccGCCATGGTCGCTGTCGGCGAACAAGACCCAGAGTGGGTTCCTGACGCGGTCGATGAAGGGCGACGGCGGTACCGCCAACTTCTTCCGCTTCGAGGACAAGGCCGGCGCCGAGCAGATCATCatgcatgc                >  6:102136/1‑133 (MQ=255)                                                                                                                     cggCCATGGTCGCTGTCGGCGAACAAGACCCCGAGTGGGTTCCTGACGCGGTCGATGAAGGGCGACGGCGGTACCGCCAACTTCTTCCGCTTCGAGGACAAGGCCGGCGCCGAGCAGATCATCATGCATGCCGAg            <  4:187929/133‑1 (MQ=255)                                                                                                                        ccATGGTCGCTGTCGGCGAACAAGACCCAGAGTGGGTTCCTGACGCGGTCGATGAAGGGCGACGGCGGTACCGCCAACTTCTTCCGCTTCGAGGACAAGGCCGGCGCCGAGCAGATCATCATGCATGCCGAgcgc         <  5:123370/135‑1 (MQ=255)                                                                                                                        ccATGGTCGCTGTCGGCGAACAAGACCCAGAGTGGGTTCCTGACGCGGTCGATGAAGGGCGACGGCGGTACCGCCAACTTCTTCCGCTTCGAGGACAAGGCCGGCGCCGAGCAGATCATCATGCATGCCGAgcg          >  1:98109/1‑134 (MQ=255)                                                                                                                           tGGTCGCTGTCGGCGAACAAGACCCAGAGTGGGTTCCTGACGCGGTCGATGAAGGGCGACGGCGGTACCGCCAACTTCTTCCGCTTCGAGGACAAGGCCGGCGCCGAGCAGATCATCATGCATGCCGAGCGCAAc      >  1:166092/1‑135 (MQ=255)                                                                                                                               cGCTGTCGGCGAACAAGACCCAGAGTGGGTTCCTGACGCGGTCGATGCAGGGCGACGGCGGTACCGCCAACTTCTTCCGCTTCGAGGACAAGGCCGGCGCCGAGCAGCTCATCATGCATGCCGAGCGCAACATgg  >  7:186869/1‑135 (MQ=255)                                                                                                                                    |                                                                                                                                  GTGGCTTCGGCAGCATCCAGATTCCGCGCGTAGGCGACGAAGTGGTGGTGGTGTTCCTGGACGGCAACCCGGACCGGCCGCTGATCATGGGCAGCCTGTACAACAGCACCAACACCCCGCCATGGTCGCTGCCGGCGAACAAGACCCAGAGTGGGTTCCTGACGCGGTCGATGAAGGGCGACGGCGGTACCGCCAACTTCTTCCGCTTCGAGGACAAGGCCGGCGCCGAGCAGATCATCATGCATGCCGAGCGCAACATGG  >  KT2440/3502724‑3502984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑