Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	KT2440	3,957,351	G→A	H100Y (CAC→TAC)	dmlR_13 ←	HTH‑type transcriptional regulator DmlR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	3,957,351	0	G	A	100.0%	183.8 / NA	51	H100Y (CAC→TAC)	dmlR_13	HTH‑type transcriptional regulator DmlR
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (25/26);  total (25/26)

CGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCA  >  KT2440/3957219‑3957482                                                                                                                                     |                                                                                                                                    cGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACg                                                                                                                                   <  8:175522/135‑1 (MQ=255) cGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACg                                                                                                                                   <  3:282838/135‑1 (MQ=255) cGGTGAGCACCACGTCATGCCCTTCGGCCAGCGGGGCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACg                                                                                                                                   <  7:109896/135‑1 (MQ=255)        caccacGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGt                                                                                                                             >  7:55831/1‑134 (MQ=255)          ccacGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGc                                                                                                                           >  7:168514/1‑134 (MQ=255)          ccacGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGc                                                                                                                           >  2:168716/1‑134 (MQ=255)          ccacGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATAACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCCGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGc                                                                                                                           >  5:159987/1‑134 (MQ=255)             cGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGCTCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAAc                                                                                                                       >  4:213657/1‑135 (MQ=255)                 aTGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAg                                                                                                                              >  8:57373/1‑124 (MQ=255)                 aTGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAg                                                                                                                              <  7:57373/124‑1 (MQ=255)                      cTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGc                                                                                                              <  6:299623/135‑1 (MQ=255)                      cTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGc                                                                                                              <  2:290430/135‑1 (MQ=255)                      cTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGc                                                                                                              <  7:18950/135‑1 (MQ=255)                      cTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGc                                                                                                              <  8:284135/135‑1 (MQ=255)                      cTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCGCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGc                                                                                                              <  1:91317/135‑1 (MQ=255)                      cTTCGGCCAGCGGGGCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGc                                                                                                              <  5:202864/135‑1 (MQ=255)                      cTTCGGCCAGCGGGGCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGc                                                                                                              <  4:253109/135‑1 (MQ=255)                          ggCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCcg                                                                                                            >  3:22851/1‑133 (MQ=255)                           gCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGa                                                                                                         >  4:241109/1‑135 (MQ=255)                            ccAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAg                                                                                                        >  1:137875/1‑135 (MQ=255)                                cGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCgcggcg                                                                                                     >  6:45333/1‑134 (MQ=255)                                  ggTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGa                                                                                                         >  7:292239/1‑128 (MQ=255)                                  ggTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGa                                                                                                         <  8:292239/128‑1 (MQ=255)                                     cGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGt                                                                                               <  3:241109/135‑1 (MQ=255)                                     cGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGt                                                                                               <  2:80361/135‑1 (MQ=255)                                                tGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCgcgc                                                                                    >  5:175330/1‑135 (MQ=255)                                                tGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTcgc                                                                                    >  3:60339/1‑135 (MQ=255)                                                tGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTcgc                                                                                    <  4:22851/135‑1 (MQ=255)                                                   gACAGCGTCAGCTCGAGCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTcgcg                                                                                   <  8:22437/133‑1 (MQ=255)                                                     cAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCACACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTc                                                                               >  2:263682/1‑135 (MQ=255)                                                     cAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTc                                                                               >  1:37259/1‑135 (MQ=255)                                                               tCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGct                                                                     >  3:294185/1‑135 (MQ=255)                                                                   tCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCG‑CCCTCGCGCCTCGCCCACGGCttcttc                                                                <  5:236500/135‑1 (MQ=255)                                                                    caccacTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCttcttc                                                                <  5:55418/135‑1 (MQ=255)                                                                     accacTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCg                                                               >  4:283909/1‑135 (MQ=255)                                                                       cacTTCCGGGTAAAGCATGTTGTAGCCGGCCACCTGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGt                                                             <  3:213657/135‑1 (MQ=255)                                                                       cacTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGt                                                             <  8:140732/135‑1 (MQ=255)                                                                                    aGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATc                                                >  1:108854/1‑135 (MQ=255)                                                                                           tgtAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCcgcg                                         >  1:215839/1‑135 (MQ=255)                                                                                           tgtAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCcgcg                                         <  3:283909/135‑1 (MQ=255)                                                                                           tgtAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCcgc                                          <  3:254049/134‑1 (MQ=255)                                                                                            gtAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCgcgc                                        >  7:32575/1‑135 (MQ=255)                                                                                             tAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCGTCGGTCAGCAGCATGATCTGCCGCGCa                                       >  4:116358/1‑135 (MQ=255)                                                                                                  ggCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTGCGGTCAGCAGCATGATCTGCCGCGCACGCTc                                  >  3:277099/1‑135 (MQ=255)                                                                                                   gCCACCAGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCACGATCTGCCGCGCACGCTcc                                 <  1:190758/135‑1 (MQ=255)                                                                                                       ccaGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCttcttc                                                                <  1:149363/100‑1 (MQ=255)                                                                                                       ccaGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCttcttc                                                                >  2:149363/1‑100 (MQ=255)                                                                                                        caGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGt                                                             <  1:122503/102‑1 (MQ=255)                                                                                                        caGCGGCATCAGCAACAGCCCCATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGt                                                             >  2:122503/1‑102 (MQ=255)                                                                                                                             cATGCCGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCAt       <  2:11078/135‑1 (MQ=255)                                                                                                                                  cGTACGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATgcgcc  >  6:169968/1‑134 (MQ=255)                                                                                                                                     |                                                                                                                                    CGGTGATCACCACGTCATGCCCTTCGGCCAGCGGGTCGGGGTTGCGCTGCGACAGCGTCAGCTCGATCACCACTTCCGGGTAAAGCATGTTGTAGCCGGCCACCAGCGGCATCAGCAACAGCCCCATGCCGTGCGGGCAGTGCAAACGCAGATGGCCGCGAGGCGTAAGGTGCGCCCCTCGCGCCTCGCCCACGGCTTCTTCGGTCAGCAGCATGATCTGCCGCGCACGCTCCAGAAACCGCTCACCCGCCTCGCTCATGCGCA  >  KT2440/3957219‑3957482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑