| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | KT2440 | 5,023,365 | T→C | S40P (TCT→CCT) | proP_6 → | Proline/betaine transporter |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 5,023,365 | 0 | T | C | 97.7% | 139.5 / ‑4.2 | 44 | S40P (TCT→CCT) | proP_6 | Proline/betaine transporter |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (19/24); total (20/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.55e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.00e-01 | |||||||||||
AAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCTCTCAATCTGAAGCCCTTTACTTCGGCCTGGCAACATTCGCTATCTCGTTCCTCATTCGACCACTTGGCGGTATGTTTTGGGGGCCGCTAGGTGACCGAATTGGCCGCAAGCGTGTGCTGGCCATGA > KT2440/5023237‑5023491 | ttatcTTGTACATGAGCATGCCTCGCAAAGCAGTGGTCGCAGCTGCATTATGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCAGCTTCATCTCAGCGGCGTTGTTTCCTGGCCCTCAAt < 1:36660/131‑1 (MQ=255)aaaGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCCCTCAAt < 5:295899/135‑1 (MQ=255) gCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCACCGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCCCTCAATCt > 6:211794/1‑134 (MQ=255) gCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCCCTCAATCTg < 8:205646/135‑1 (MQ=255) gCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCCCTCAATCTg < 6:84216/135‑1 (MQ=255) gCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTAAGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCCCTCAATCTg < 6:61036/135‑1 (MQ=255) aCATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCCCTCAATCTGAAGccc > 5:80713/1‑135 (MQ=255) cATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCACCCCGACGGAATCGTTCGATTACGGAATCTAAGCTTACGGGCTCACCTTCATATCAGCCTCCTTGTTTCCAGGCCCTCAAACTGAAGccag > 5:167152/1‑133 (MQ=255) cATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCCCTCAATCTGAAGCCCt > 1:182044/1‑135 (MQ=255) cATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGAGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTAAGCCGCCTTGTTTCCAGGCCCTCAATCAGAAGCCCt > 7:105483/1‑135 (MQ=255) cTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCCCTCAATCTGAAGCCCTTTACTTCGGcc > 6:248683/1‑135 (MQ=255) gCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCCCTCAATCTGAAGCCCTTTACTTCGGCCTg > 4:280230/1‑134 (MQ=255) tCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCCCTCAATCTGAAGCCCTTTACTTCGGCCTGGCAACATTCGCTa < 6:83782/135‑1 (MQ=255) tCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCCCTCAATCTGAAGCCCTGTACTTCGGCCTGGCAACATTCGCTa > 4:110282/1‑135 (MQ=255) cGCAGCTGCATTAGGCAACGCGACGTAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCCCTCAATCTGAAGCCCTTTACTTCGGCCTGGCAACATTCGCTAt > 6:130201/1‑135 (MQ=255) aGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCCCTCAATCTGAAGCCCTTTACTTCGGCCTGGCAACATTCGCTAtctc < 8:303021/135‑1 (MQ=255) gCTGCATTAGGCAACGCGACGGAATGGTTCTATTACGGAATCTATGCTTACGGGCTCCCCTTCATCTCAGCCGCCTTGTTTCCTGGCCCTCAATCTGAAGCCCTTTACTTCGGCCTGGCAACATTCGCTATCTCg > 4:216494/1‑135 (MQ=255) gCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCCCTCAATCTGACGCCCTTTACTTCGTCCTGGCAACATTCGCTATCTCGTTc > 6:239897/1‑135 (MQ=255) cgcgACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCGCACCTTCATCTCAGCCGCCTTGTTTCCTGGCCCTCAATCTGAAGCCCTTTACTTCGGCCTGGCAACATTCGCTATCTCGTTCCTCATTCGAcc < 6:11696/135‑1 (MQ=255) aaTGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCCCTCAATCTGAAGCCCTTTACTTCGGCCTGGCAACATTCGCTATCTCGTTCCTCATTCGACCACTTGGCg > 3:193147/1‑135 (MQ=255) aaTGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCCCTCAATCTGAAGCCCTTTACTTCGGCCTGGCAACATTCGCTATCTCGTTCCTCATTCGACCACTTGGCg > 1:301390/1‑135 (MQ=255) aTTACGGAATCTATGCTTACGGGCTCACCTTCTTCTCAGCCGCCTTGTTTCCTGGCCCTCAATGTGAAGCCCTTTACTTCGGCCTGGCAACATTCGCTATCTCGTTCCTCATTCTACCACTTGGCGGTATGtttt < 1:151170/135‑1 (MQ=255) cGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCTCTCAATCTGAAGCCCTTTACTTCGGCCTGGCAACAGTCGCGATCTCGGTCGTCATTCGACCACTAGGCGGTATGTTTTgggg > 1:149708/1‑135 (MQ=255) cGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCCCTCAATCTGAAGCCCTTTACTTCGGCCTGGCAACATTCGCTATCTCGTTCCTCATTCGACCACTTGGCGGTATGTTTTgggg > 7:259619/1‑135 (MQ=255) ggAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCCCTCAATCTGAAGCCCTTTACTTCGGCCTGGCAACATTCGCTATCTCGTTCCTCATTCGACCACTTGGCGGTATGTTTTggggg < 5:190697/135‑1 (MQ=255) aTCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCCCTCAATCTGAAGCCCTTTACTTCGGCCTGGCAACATTCGCTATCTCGTTCCTCATTCGACCACTTGGCGGTATGTTTTGGGGGCCg > 3:186650/1‑135 (MQ=255) aTGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCCCTCAATCTGAAGCCCTTTACTTCGGCCTGGCAACATTCGCTATCTCGTTCCTCATTCGACCACTTGGCGGTATGTTTTGGGGGCCGCTAg > 4:208664/1‑135 (MQ=255) gCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCCCTCAATCTGAAGCCCTTTACTTCGGCCTGGCAACATTCGCTATCTCGTTCCTCATTCGACCACTTGGCGGTATGTTTTGGGGGCCGCTAGGTGACCGAAt > 3:174877/1‑135 (MQ=255) gCTCACCTTCATCTCAGCCGCCATGTTTCCTGGCCCTCAATCTGAAGCCCTATACTTCGGCCTGGCAACATTAGCTATCTCGTTACTCATTAGACCACATGGAGGGATGTTTTTTGGGCCGCTAGGTGAcacaaa > 7:257871/1‑130 (MQ=255) ccTTCATCTCAGCCGCCTTGTTTCCTGGCCCTCAATCTGAAGCCCTTTACTTCGGCCTGGCAACATTCGCTATCTCGTTCCTCATTCGACCACTTGGCGGTATGTTTTGGGGGCCGCTAGGTGACCGAATTgg < 1:51176/133‑1 (MQ=255) gccgccTTGTTTCCTGGCCCTCAATCTGAAGCCCTTTACTTCGGCCTGGCAACATTCGCTATCTCGTTCCTCATTCGACCACTTGGCGGTATGTTTTGGGGGCCGCTAGGTGACCGAATTGGCCGCAAGCgt < 1:248007/132‑1 (MQ=255) gccgccTTGTTTCCTGGCCCTCAATCTGAAGCCCTTTACTTCGGCCTGGCAACATTCGCTATCTCGTTCCTCATTCGACCACTTGGCGGTATGTTTTGGGGGCCGCTAGGTGACCGAATTGGCCGCAAGCgt < 2:301390/132‑1 (MQ=255) cgccTTGTTTCCTGGCCCTCAATCTGAAGCCCTTTACTTCGGCCTGGCAACATTCGCTATCTCGTTCCTCATTCGCCCACTTGGCGGTATGTTTTGGGGGCCGCGAGGTGACCGAATTGGCCGCAAGCGTGTGCt > 6:22749/1‑135 (MQ=255) cgccTTGTTTCCTGGCCCTCAATCTGAAGCCCTTTACTTCGGCCTGGCAACATTCGCTATCTCGTTCCTCATTCGACCACTTGGCGGTATGTTTTGGGGGCCGCTAGGTGACCGAATTGGCCGCAAGCGTGTGCt < 7:259224/135‑1 (MQ=255) cTTGTTTCCTGGCCCTCAATCTGAAGCCCTTTACTTCGGCCTGGCAACATTCGCTATCTCGTTCCTCATTCGACCACTTGGCGGTATGTTTTGGGGGCCGCTAGGTGACCGAATTGGCCGCAAGCGTGTGCTGGc < 3:208664/135‑1 (MQ=255) tttCCTGGCCCTCAATCTGAAGCCCTTTACTTCGGCCTGGCTACATTCGCTATCTCGTTCCTCATTCGACCACTTGGCGGTATGTTTTGGGGGCCGCTAGGTGACCGAATTGGCCGCAAGCGTGTGCTGGCCa < 4:128237/133‑1 (MQ=255) tttCCTGGCCCTCAATCTGAAGCCCTTTACTTCGGCCTGGCAACATTCGCTATCTCGTTCCTCATTCGACCACTTGGCGGTATGTTTTGGGGGCCGCTAGGTGACCGAATTGGCCGCAAGCGTGTGCTGGCCa < 4:174877/133‑1 (MQ=255) tttCCTGGCCCTCAATCTGAAGCCCTTTACTTCGGCCTGGCAACATTCGCTATCTCGTTCCTCATTCGACCACTTGGCGGTATGTTTTGGGGGCCGCTAGGTGACCGAATTGGCCGCAAGCGTGTGCTGGCCa < 8:259619/133‑1 (MQ=255) tttCCTGGCCCTCAATCTGAAGCCCTTTACTTCGGCCTGGCAACATTCGCTATCTCGTTCCTCATTCGACCACTTGGCGGTATGTTTTGGGGGCCGCTAGGTGACCGAATTGGCCGCAAGCGTGTGCTGGCCa < 5:130201/133‑1 (MQ=255) tttCCTGGCCCTCAATCTGAAGCCCTTTACTTCGGCCTGGCAACATTCGCTATCTCGTTCCTCATTCGACCACTTGGCGGTATGTTTTGGGGGCCGCTAGGTGACCGAATTGGCCGCAAGCGTGTGCTGGCCa < 5:46377/133‑1 (MQ=255) tttCCTGGCCCTCAATCTGAAGCCCTTTACTTCGGCCTGGCAACATTCGCTATCTCGTTCCTCATTCGACCACTTGGCGGTATGTTTTGGGGGCCGCTAGGTGACCGAATTGGCCGCAAGCGTGTGCTGGCCa < 3:216494/133‑1 (MQ=255) tttCCTGGCCCTCAATCTGAAGCCCTTTACTTCGGCCTGGCAACATTCGCTATCTCGTTCCTCATTCGACCACTTGGCGGTATGTTTTGGGGGCCGCTAGGTGACCGAATTGGCCGCAAGCGTGTGCTGGCCa < 3:280230/133‑1 (MQ=255) tttCCTGGCCCTCAATCTGAACCCCTTTACTTCGGCCTGGCAACATTCGCTATCTCGTTCCTCATTCGACCACTTGGCGGTATGTTTTGGGGGCCGCTAGGTGACCGAATTGGCCGCAAGCGTGTGCTGGCCa < 8:105483/133‑1 (MQ=255) ttCCTGGCCCTCAATCTGAAGCCCTTTACTTCGGCCTGGCAACATTCGCTATCTCGTTCCTCATTCGACCACTTGGCGGTATGTTTTGGGGGCCGCTAGGTGACCGAATTGGCCGCAAGCGTGTGCTGGCCATGa < 5:248683/135‑1 (MQ=255) | AAAGCTTGAACATGAGCATGCCTCGCAAAGCCGTGGTCGCAGCTGCATTAGGCAACGCGACGGAATGGTTCGATTACGGAATCTATGCTTACGGGCTCACCTTCATCTCAGCCGCCTTGTTTCCTGGCTCTCAATCTGAAGCCCTTTACTTCGGCCTGGCAACATTCGCTATCTCGTTCCTCATTCGACCACTTGGCGGTATGTTTTGGGGGCCGCTAGGTGACCGAATTGGCCGCAAGCGTGTGCTGGCCATGA > KT2440/5023237‑5023491 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |