Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT2440434,3270AC36.4% 64.0 / 10.9 45H53P (CAC→CCC) bioF8‑amino‑7‑oxononanoate synthase
Reads supporting (aligned to +/- strand):  ref base A (9/19);  new base C (8/8);  total (17/28)
Fisher's exact test for biased strand distribution p-value = 3.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGCCTGGCCGAACGGCGCGCCGCAGACCTGTATCGGCAGCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGT‑GT‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCC  >  KT2440/434191‑434462
                                                                                                                                        |                                                                                                                                         
ggcCTGGCCGAACGGCGCGCCGCAGACCTGTATCGGCAGCGGCCATTGCTGGAACGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAacca                                                                                                                                           <  7:331073/136‑1 (MQ=255)
  ccTGGCCGAACGGCGCGCCGCAGACCTGTATCGGCAGCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAaccacc                                                                                                                                         <  2:177424/137‑1 (MQ=255)
                  gccgcAGACCTGTATCGGCAGCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCCGCGGTTGCTGGCCGTCTGCAGCAATGACTACCTGGGCCTGGCCAACCCCCCCGCCGTGATcgccgc                                                                                                                         >  8:344475/1‑137 (MQ=255)
                  gccgcAGACCTGTATCGGCAGCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCCGCGGGTGGGGGGCGTCTGGCGCCAAGCCGACCTGGGCCGGGCCCACCCCCCCCCGGGGATcgcccc                                                                                                                         >  6:162645/1‑135 (MQ=255)
                   ccgcAGACCTGTATCGGCAGCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCgccgcc                                                                                                                        <  2:4026/137‑1 (MQ=255)
                   ccgcAGACCTGTATCGGCAGCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCgccgcc                                                                                                                        <  2:2593/137‑1 (MQ=255)
                    cgcAGACCTGTATCGGCAGCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGAGGGGCCGCGGGTGGTGGGCGTGTGCCGCCATGTCTACCAGGGCCGGGGCCACCCCCCCGCCGGGTTCgccgccc                                                                                                                       >  3:361167/1‑136 (MQ=255)
                             gTATCGGCAGCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCg                                                                                                              <  2:334952/137‑1 (MQ=255)
                               aTCGGCTGCGGCCATTGCTGGACAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGgc                                                                                                            <  1:19912/137‑1 (MQ=255)
                               aTCGGCAGCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGgc                                                                                                            <  8:478775/137‑1 (MQ=255)
                               aTCGGCAGCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGgc                                                                                                            <  3:202192/137‑1 (MQ=255)
                               aTCGGCAGCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCACCCGGTGCTGGGCGTCTGCAGCCATTACGACCGGGGCCGGGCCCACCCCCCCCCGGTGTTCGCCGCCAGGCCGGCCCgc                                                                                                            >  3:281920/1‑137 (MQ=255)
                                 cGGCAGCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCCCCGGTTGCCTGGCGTCGGGACCAATGTCAACCTGGGCCGGGCCCACCCCCCCCAGGTGAGCCCCCCCGCGCAGGCCGgcgc                                                                                                          >  4:365887/1‑137 (MQ=255)
                                 cGGCAGCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGgcgc                                                                                                          <  1:406830/137‑1 (MQ=255)
                                    cAGCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGa                                                                                                       <  4:115859/137‑1 (MQ=255)
                                      gCGGCCTTTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGGCAGCGGTTGCTGGCCGTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAgc                                                                                                     <  6:111879/137‑1 (MQ=255)
                                      gCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTTCTGGCCTTGTGCAGCAATGACTACCTGGGCCGTGCCAACCCCCCCCAGGGGATCGCCGCCTGGCAGGCCCGCGCCGAgc                                                                                                     >  5:405928/1‑137 (MQ=255)
                                      gCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAgc                                                                                                     <  4:185936/137‑1 (MQ=255)
                                      gCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAgc                                                                                                     <  1:199553/137‑1 (MQ=255)
                                      gCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGGTGCTGGCCTTCTTCAGCAATTACTACCTGGGCCGGGCCCACCCCCCCGAGGTTATCGCCGCCGGGCAGGCCCGCGCCCAgc                                                                                                     >  5:398672/1‑137 (MQ=255)
                                       cGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAgcg                                                                                                    <  4:467021/137‑1 (MQ=255)
                                       aggCCATTGCTGTTAAGCGCTCAAGGGGCTGATGTGGTTGTCGTAGGCCAGCGGTTGGTGGGCTTCTGCAGCTATGACTACCTGGGCCTGGCCAACCTCGCCGAGTTGATCTCGGCATGGCAGGCGGGGGGAGAgcg                                                                                                    <  4:1677/136‑1 (MQ=255)
                                        ggCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGGCCCCCGTTTGGGGCGGTGTGGGGCAAATCCGAACTGGGGCGGGCCCACCCCCCCCCGGGGGTCGCCGCCCGGCCGGCCGGCGgcgcgccc                                                                                                   >  8:179683/1‑135 (MQ=255)
                                        ggCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCCGCGGTTGCGGGGCGTAGGCCGCCATGACGAACGGGGCCAGGCCAACCACCCCCAGGTGATCGCCCCCGGGCAGGCCGGCGCCGAgccc                                                                                                   >  4:482588/1‑135 (MQ=255)
                                         gCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCt                                                                                                  <  2:104420/137‑1 (MQ=255)
                                         gCCATTGCGGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGTAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCt                                                                                                  <  2:113212/137‑1 (MQ=255)
                                          ccATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGGCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTg                                                                                                 <  6:112646/137‑1 (MQ=255)
                                           cATTGCTGGAAAGCCCGCACGGGCCGGAAGTGGTGGGCGACGGCCAGCGGTTGCGGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTgg                                                                                                <  8:421206/137‑1 (MQ=255)
                                                       gCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGGCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGT‑GT‑ggggggg                                                                                  >  6:501219/1‑137 (MQ=255)
                                                           gCAAGGGCCGGAAGGGGTGGGCGCCGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGT‑GT‑ggggggggggc                                                                              <  7:170498/137‑2 (MQ=255)
                                                             aaGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTTGGGGGCGTGTGCAGCCAATACTACCAGGGCCTGGCCCACCAACCCCCGGTTATAGCCCCCAGGCAGGCCGGCCCCCCGCGCGGGGGTGGT‑GGGGGGGGGGGc                                                                             >  6:436079/1‑137 (MQ=255)
                                                             aaGGGCCGGAAGTGGTGGTCGACGGCCAGCCGTTTCTGGCCTTCTGCAGCAATTACTAACTGGGCCTGGCCAACCACCCCGAGGTGATAGCCGCCTGGCAGGCAGGCGCCGAGGGCTGGGGT‑GG‑GGGGGGGGGGGcc                                                                            >  2:371434/1‑137 (MQ=255)
                                                                                              tgggggCCTTCGGCACCAATGACCACCTGGGCCTGGCCAACCCCCCCGGGGTTTCCGCCGGCGGGCCGGCCGGCGCCGCGGGCGGGGGG‑GG‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCAc                                           <  8:437860/133‑1 (MQ=255)
                                                                                              tGCTGGCCTGGTGCCCCAAGTCCTACCGGGGCCGGGCCACCCCCCCCGCGGGTCTCCGCGGCTGGCCGGGCGGCGCCGAGGGGTGGGGG‑GT‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCAc                                           <  2:77893/137‑1 (MQ=255)
                                                                                              tGCGGGCCTTGTGCACCCATTCCCACCTGGGCCTGGCCAACCCCCCCGAGGGTCTCGCCCCCGGGCCGGCCGGGGCCGCGCGGGGGGGG‑GG‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCAc                                           <  4:281920/137‑1 (MQ=255)
                                                                                              ggCTGGCCTTCTGCACCAATGACTACCTGGGCCTGGCCAACCCCCCCGAGGTGAACGCCGCCTGGCAGGCCGGCGCCGAGCGCGGGGGT‑GT‑‑GGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCAcc                                          <  2:264865/136‑1 (MQ=255)
                                                                                                              caATGACAACCTGCGGCTGGCCAACCACCGCGTGGTTGTCGCCGCCTGGCAGGCGGGGGGCGCGCGGGGGGGT‑GT‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCAGAGCACCCCGCACCATCAGGTTGAAGAgg                           <  4:451946/137‑1 (MQ=255)
                                                                                                              aaaTGACTACCGGGGCGGGGCCAACCCCCCCGGGGTTTCGGCCGCGGGGCGGGCGGGGGCCGCGCGGGGGGGT‑GG‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAgg                           <  7:344475/136‑1 (MQ=255)
                                                                                                              aaaTGACAACCTGGGCGTGGCCAACCCCCCCGTGGGGTGCGCCGGCGGGGAGGGCGGGGGCGTGGGGTGGGGT‑GG‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAgg                           <  5:162645/136‑1 (MQ=255)
                                                                                                                    ccccccgggccgggccAACCCCCCCGCGGGGACCGCCCGCGGGCCGGCCGGGCGCGCCGGGTGGGGG‑GG‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTgg                     <  3:371521/131‑1 (MQ=255)
                                                                                                                      aCCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCTGCGCCGAGCGCTGGGGT‑GT‑GGGGGGGGGGGCCTCGCACAGGGAAGTTGCCCCCCCCCCCCCGCACCATCTGGTTGAAGCGGCACTGGCt                   >  6:470817/1‑137 (MQ=255)
                                                                                                                               tGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGgc                                                                                                            <  1:191231/41‑1 (MQ=255)
                                                                                                                               tGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGgc                                                                                                            >  2:191231/1‑41 (MQ=255)
                                                                                                                               ggcccAACCCCCCCGCGGGGATCCCCCCCGGGCCGGCCGGCGCCGCGCGGGGGGGG‑GG‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCAcc          <  4:361167/134‑1 (MQ=255)
                                                                                                                                  ccAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGT‑GT‑GGGGGGGGGGGCCGCGCCCCTGGTAGTTGGCCACCGCCCCCCGCACCATCAGGTTGAAGAGGCACGGGCTGAACTAACCGgc       >  1:64071/1‑137 (MQ=255)
                                                                                                                                  ccAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGT‑GT‑‑GGGGGGGGGGCCTCGCCCCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGgcc      >  3:250860/1‑137 (MQ=255)
                                                                                                                                    aaCCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGT‑GT‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGgccg     >  1:461095/1‑137 (MQ=255)
                                                                                                                                      ccaccCCGCGGTTCTCGCGGACGGGGCGGCCGGCGCCGCGGGGGGGGGT‑GG‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGgcctcc   <  6:195035/137‑1 (MQ=255)
                                                                                                                                        accCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGT‑GTGGGGGGGGGGGGCCTCGCACCTGGTTGTTGGCCACCGCCCCCCGCACCAGCAGGTTGAAGAGGCACTGGCTGAACTCACCGGccgccc  >  3:324461/1‑137 (MQ=255)
                                                                                                                                        |                                                                                                                                         
CGCCTGGCCGAACGGCGCGCCGCAGACCTGTATCGGCAGCGGCCATTGCTGGAAAGCCCGCAAGGGCCGGAAGTGGTGGTCGACGGCCAGCGGTTGCTGGCCTTCTGCAGCAATGACTACCTGGGCCTGGCCAACCACCCCGAGGTGATCGCCGCCTGGCAGGCCGGCGCCGAGCGCTGGGGT‑GT‑GGGGGGGGGGGCCTCGCACCTGGTAGTTGGCCACAGCACCCCGCACCATCAGGTTGAAGAGGCACTGGCTGAACTCACCGGCCGCCC  >  KT2440/434191‑434462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑