Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT2440783,2980GC33.9% 100.4 / 11.1 56P141R (CCG→CGG) greB_1Transcription elongation factor GreB
Reads supporting (aligned to +/- strand):  ref base G (5/32);  new base C (19/0);  total (24/32)
Fisher's exact test for biased strand distribution p-value = 1.00e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAG‑ACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAACCCCAGTTGATCAACCCGTGCCCGGCATCCGCCTCATCCTCGCCCACCAGCTGCACCACCTGCTCCTGGTCCTGCGCGTCGACAAACCGCACCCGGC  >  KT2440/783170‑783430
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ccGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCCGCCGCCGCCCG‑ACCACCCCAGCCCCCCGccccccc                                                                                                                              >  4:342573/1‑133 (MQ=255)
ccGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTACCCCACCCGCCGCCGCCCC‑CCCACCCCAAACCCCCGccccccc                                                                                                                              >  8:285291/1‑133 (MQ=255)
ccGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCGATACCCTGTTCCCCCCACCGCCGCCGCCAC‑CCCACCTCAGCCCCCCGccccccc                                                                                                                              >  8:128498/1‑133 (MQ=255)
      ggggCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCCCCTCTATCACCTGTTGCCCCACCGGCCGCCGCCAG‑AACACCAGCTCCCACCgccccgccccccgc                                                                                                                        >  6:333450/1‑133 (MQ=255)
      ggggCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGGTCCCCCACCGGCCGCCGCCAC‑ACCACCGCCTCCCCCCGCcccccccccccc                                                                                                                        >  4:247072/1‑133 (MQ=255)
       gggCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAG‑ACCACCTCATCCCCCGGCCCAGCCCCGAGCa                                                                                                                       <  2:36465/137‑1 (MQ=255)
         gCCGCTTTGCGGCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAG‑ACCACCTCATCCCCCGGCCCAGCCCCGAGCaaa                                                                                                                     <  3:29226/137‑1 (MQ=255)
              tttGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCCCCGGCCGCCGCCCG‑CCCCCCTCATCCCCCCGCCCCCCCCCGCGCCAAccccc                                                                                                                >  5:402907/1‑136 (MQ=255)
              tttGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCCCCGGCCGCCGCCAG‑CCCACCTCATCCCCCCGCCCCGCCCCCCGCAAAgcccc                                                                                                                >  5:233613/1‑136 (MQ=255)
                tGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTGCCCCCCCGGCCGCCGCCCG‑ACCACCGCATCCCCCCGCCCCCCCCCGCGCCAAgcccccc                                                                                                              >  2:125899/1‑137 (MQ=255)
                         ttGGGATGCAAAGAAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAG‑ACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCg                                                                                                     <  7:128498/136‑1 (MQ=255)
                          tGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCCCCCGCCGCCGCCCG‑ACCCCCACAACCCCCCGCCCACCCCCCCGCCAACCCCCCCCCCAAGGCgc                                                                                                    >  2:374593/1‑136 (MQ=255)
                              cTGCACAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAG‑ACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAAccc                                                                                                <  1:437415/137‑1 (MQ=255)
                              cTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAG‑ACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAAccc                                                                                                <  1:269010/137‑1 (MQ=255)
                                  aaaGCCGCCCCAGGGCCCTCAACCCACGCCCCGAATCTCAACAACCTCTATGACCTGTTAACCCCCCGGCCGACGACAG‑ACCACCACAACACCCCGCCCAGCCCCCCGCAACGACCCCCCCAAAGCCGAACCCCAgg                                                                                            >  2:338466/1‑136 (MQ=255)
                                   aaGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAG‑ACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAACCCCAGtt                                                                                           <  2:8380/137‑1 (MQ=255)
                                    cgcagcCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAG‑ACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAACCCCAGTTg                                                                                          <  6:121313/136‑1 (MQ=255)
                                         ccccAGGGCCCTCACCCCTCGCCCAGAATCTCAACCCCCTCGATCACCTGTTGCCCCACCCGCCGCCGCCAGCACCA‑CACATCCCCCCGCACAGCCCCAAGCCAAGCCCCCCCCAAAGCCGCACCCCACGTGCTCaa                                                                                     >  8:314272/1‑137 (MQ=255)
                                          cccAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAG‑ACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAACCCCAGTTGATCAAc                                                                                    <  3:421707/137‑1 (MQ=255)
                                            cAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAG‑ACCCCCTCATCCCCCGGCCCCGCCCCGCGCCAAGCCCGCCCCCATGGCGCCCCCCAGGTGGACAAccc                                                                                  >  8:36792/1‑137 (MQ=255)
                                            cAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAG‑ACCACCTCATCCCCCCGCCCCGCCCCGCCCAAAGCCCGCCCCAATGGCGACCCCCCGGTGCTCCAccc                                                                                  >  2:408256/1‑137 (MQ=255)
                                              gggCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAG‑ACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAACCCCAGTTGATCAACCCGt                                                                                <  7:113497/137‑1 (MQ=255)
                                              gggCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAG‑ACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAACCCCAGTTGATCAACCCGt                                                                                <  5:92128/137‑1 (MQ=255)
                                                 cccTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCCCCGGCCGCCGCCAC‑ACCACCTCATCCCCCCGCCCACCCCCCACCCAACCCCGCCCCAAAGGCGAACCCCAGTTGATCAACCCGTGcc                                                                             >  3:162285/1‑137 (MQ=255)
                                                    tCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAG‑ACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAACCCCAGTTGATCAACCCGTGCCCgg                                                                          <  5:482305/137‑1 (MQ=255)
                                                     cAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAG‑ACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAACCCCAGTTGATCAACCCGTGCCCGGc                                                                         <  7:243969/137‑1 (MQ=255)
                                                          cTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAG‑ACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAACCCCAGTTGATCAACCCGTGCCCGGCATCCg                                                                    <  1:374593/137‑1 (MQ=255)
                                                          cTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAG‑ACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAACCCCAGTTGATCAACCCGTGCCCGGCATCCg                                                                    <  4:379505/137‑1 (MQ=255)
                                                              cccAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAG‑CCCACCTCATACCCCCGCCCCCCCCCGCGCAAAGCCCCCCCCAATGGCGCACCCCACTTGATAAACCCGGGCCCCGCCTCCCCCTc                                                                >  6:188700/1‑137 (MQ=255)
                                                              cccAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAG‑ACCACCTCATCCCCCCGCCCCGCCCCCCGCAAATCCCCCCCCAAAGGCGCACCCCACTTGATCAAACCCTGCCCGGCAACCGccac                                                                >  3:11175/1‑135 (MQ=255)
                                                                cAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAG‑ACCACCTCCTCCCCCGGCCCCGCCCCCAGCCAACCCCGCCCCCAAGGCGCACCCCAGTTGATCAACCCCTGCCCCGCCCCCGCcacaa                                                              >  2:367337/1‑136 (MQ=255)
                                                                cAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAG‑ACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAAAGGCGAACCCCAGTTGATAAACCCGGGCCCCGCATCCGCCTCAt                                                              >  2:45971/1‑137 (MQ=255)
                                                                     tctcAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAG‑CCCACCTCATCCCCCCGCCCCCCCCCCAGCCAACCCCGCCCCACTAGCGAAACCCAGTTGATCAACCCGGGCCCCGCATACGCCTCAACATCg                                                         >  8:212303/1‑137 (MQ=255)
                                                                        catccaacTCTATCACCTGTTCCCCCACCGTTCTCCGCCAG‑ACCTCCTCATCCCCCGGCCCAGCCCCGAGCAAAGCACGCCCCAATGGCGAACGCCAGTTGATCAACCCGTGCCCTGCATCCGCCTCATCCTCGccc                                                      <  8:490287/134‑1 (MQ=255)
                                                                            caccTCTATCACCTGTTCCCCCACCGGCCGCCGCCAG‑ACCACCTCATCCCCCCGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGTACCCCAGTTGATAACACCGTGCCCCGCCTCCCCGGCATCCTCGCccaccc                                                  >  8:437768/1‑136 (MQ=255)
                                                                                tctATCACCTGTTCCCCCACCGGCCGCCGCCAG‑ACCACCTCATCCCCCGGCCCa                                                                                                                                 >  2:469691/1‑54 (MQ=255)
                                                                                tctATCACCTGTTCCCCCACCGGCCGCCGCCAG‑ACCACCTCATCCCCCGGCCCa                                                                                                                                 <  1:469691/54‑1 (MQ=255)
                                                                                tctATCACCTGTTCCCCCACCGGCCGCCGCCAG‑ACCACCTCATCCCCCCGCCCCGCCCCGCGCAAACCCCCCCCCAAAGGCGCAACCCAATTGATAAACCCCTGCCCCGCAACCGCCACAACAGCGCCCACAAGcag                                              >  8:48574/1‑135 (MQ=255)
                                                                                   aTCACCTGTTCCCCCACCGGCCTCCGCCAG‑ACCAACTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAACCCCAGTTGATCAACCCGTGCCCGGCATCCGCCTCATCCTCGCCCACCAGCTGcac                                           <  2:83693/137‑1 (MQ=255)
                                                                                   aTCACCTGTTCCCCCACCGGCCGCCGCCAG‑ACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAACCCCAGTTGATCAACCCGTGCCCGGCATCCGCCTCATCCTCGCCCACCAGCTGcac                                           <  8:192113/137‑1 (MQ=255)
                                                                                    tCACCTGTTCCCCCACCGGCCGCCGCCAG‑ACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATTGCGAACCCCAGTTGATCAACCCGTGCCCGGCATCCGCCTCATCCTCGCCCACCAGCTGcacc                                          <  5:460488/137‑1 (MQ=255)
                                                                                    tCACCTGTTCCCCCACCGGCCGCCGCCAG‑ACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAACCCCAGTTGATCAACCCGTGCCCGGCATCCGCCTCATCCTCGCCCACCAGCTGcacc                                          <  1:125899/137‑1 (MQ=255)
                                                                                    tCACCTGTTCCCCCACCGGCCGCCGCCAG‑ACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAACCCCAGTTGATCAACCCGTGCCCGGCATCCGCCTCATCCTCGCCCACCAGCTGcacc                                          <  3:92367/137‑1 (MQ=255)
                                                                                     cACCTGTTCCCCCACCGGCCGCCGCCAG‑ACCACCTCATCCCACGGCCCAGCCCCGAGCAAATCCCGCCCCAATGGCGAACCCCAGTTGATCAACCCGTGCCCGGCATCCGCCTCATCCTCGCCCACCAGCTGcacca                                         <  1:348380/137‑1 (MQ=255)
                                                                                       ccTGTTCCCCCACCGGCCGCCGCCAG‑ACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAACCCCAGTTGATCAACCCGTGCCCGGCATCCGCCTCATCCTCGCCCACCAGCTGCaccacc                                       <  1:408256/137‑1 (MQ=255)
                                                                                          gTTCCCCCACCGGCCGCCGCCAG‑ACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAACCCCAGTTGATCAACCCGTGCCCGGCATCCGCCTCATCCTCGCCCACCAGCTGCACCACCTGc                                    <  1:296287/137‑1 (MQ=255)
                                                                                                    cGGCCGCCGCCAG‑ACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAACCCCAGTTGATCAACCCGTGCCCGGCATCCGCCTCATCCTCGCCCACCAGCTGCACCACCTGCTCCTGGTCCt                          <  7:285291/137‑1 (MQ=255)
                                                                                                    cGGCCGCCGCCAG‑ACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAACCCCAGTTGATCAACCCGTGCCCCGCATCCGCCTCATCCTCGCCCACCAGCTGCACCACCTGCTCCTGGTCCt                          <  3:342573/137‑1 (MQ=255)
                                                                                                    cGGCCGCCGCCAG‑ACCACCTCATCCCCCGGCACATCCCCGAGCAACGCCCTCCCCAATGGCGAACCCGAGTTGATCAACCCGTGCCCGGCATCCGACTCATCCTCGCCCACCAGCTGCACCACCTGCTCCTGGTCCt                          <  2:390885/137‑1 (MQ=255)
                                                                                                        ccccgccAG‑ACCACCTCATCCCCCCGCCCCGCCCCCACCCAAGCCCCCCCCAAAGGCCGACCCCCCTTGCACCACCCCAGCACCGCCACCCCCACAAGCTCCCCCCCCCGCGGGCCCCCCGGCTCCTGGGCCTgcgc                      >  4:257073/3‑137 (MQ=255)
                                                                                                                   ccaccTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAACCCCAGTTGATCAACCCGTGCCCGGCATCCGCCTCATCCTCGCCCACCAGCTGCACCACCTGCTCCTGGTCCTGCGCGTCGACAAAc            <  7:36792/137‑1 (MQ=255)
                                                                                                                       cTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAACCCCAGTTGATCAACCCGTGCCCGGCATCCGCCTCATCCTCGCCCACCAGCTGCACCACCTGCTCCTGGTCCTGCGCGTCGACAAACCGCa        <  3:313413/137‑1 (MQ=255)
                                                                                                                         aaTCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAACCCCAGTTGATCAACCCGTGCCCGGCATCCGCCTCATCCTCGCCCACCAGCTGCACCACCTGCTCCTGGTCCTGCGCGTCGACAAACCGCAcc      >  5:203030/2‑137 (MQ=255)
                                                                                                                            cccccGGCCCAGCCCCGAGCAAAGCCCTCCCCAATGGCGAAACCCAGTTGATGAACACTGTCCCGGCATCCTCCTCATCCTCGGCCTAGATCTGCACCAACTGCTCCTGGTACTGCGCGTCGACATACCTCACCCgg   <  1:208445/137‑1 (MQ=255)
                                                                                                                            cccccGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAACCCCAGTTGATCAACCCGTGCCCGGCATCCGCCTCATCCTCGCCCACCAGCTGCACCACCTGCTCCTGGTCCTGCGCGTCGACAAACCGCACCCgg   <  5:333450/137‑1 (MQ=255)
                                                                                                                             ccccGGCCCAGCCACGAGCAAAGCCCGCCCCAATGGCGAACCCCAGTTGATCAACCCGTGCCCGGCATCCGCCTCATCCTCGCCCACCAGCTGCACCACCTGCTCCTGGTCCTGCGCGTCGACAAACCGCACCCGGc  <  1:259729/137‑1 (MQ=255)
                                                                                                                                 |                                                                                                                                    
CCGTAGGGGGCCGCTTTGCGTCGCGATGGGCTGCAAAGCAGCCCCAGGGCCCTCAACCCTCGCCCAGAATCTCAACCACCTCTATCACCTGTTCCCCCACCGGCCGCCGCCAG‑ACCACCTCATCCCCCGGCCCAGCCCCGAGCAAAGCCCGCCCCAATGGCGAACCCCAGTTGATCAACCCGTGCCCGGCATCCGCCTCATCCTCGCCCACCAGCTGCACCACCTGCTCCTGGTCCTGCGCGTCGACAAACCGCACCCGGC  >  KT2440/783170‑783430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑