Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24402,578,0500AC36.1% 41.9 / 10.3 36S168A (TCC→GCC) folD_2Bifunctional protein FolD protein
Reads supporting (aligned to +/- strand):  ref base A (20/3);  new base C (0/13);  total (20/16)
Fisher's exact test for biased strand distribution p-value = 2.42e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGCTTGCCGGCAGCCACGACCACCAGGTCGGCACGCCCGACGTGGCCGGCCAGGTCCTTGGTGAAGCGGTGGCAGACGGTCACGGTGCAGCCGGCCAGCAGCAGTTCCATGGCCATCGGGCGGCCAACGATGTTGGACGCGCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGCCGGTGCTTTCCAGCAGGGTCATGATGCCTTTCGGTGTGCACGGGCGCAGCAGTGGAATACGCTGGGCCAGGCGGCCGATGTTGTACGGGTGGA  >  KT2440/2577914‑2578184
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ggCTTGCCGGCAGCCACGACCACCAGGTCGGCACGCCCGACGTGGCCGGCCAGGTCCTTGGTGAAGCGGTGGCAGCCGGTCACGGTGCAGCCGGCCAGCAGCAGTTCCATGGCCATCGGGCGGCCAACGATGTTGGa                                                                                                                                        >  5:155506/1‑137 (MQ=255)
                 gACCACCAGGTCGGCACGCCCGACGTGGCCGGCCAGGTCCTTGGTGAAGCGGTGGCAGACGGTCACGGTGCAGCCGGCCAGCAGCAGTTCCATGGCCATCGGGCGGCCAACGATGTTGGACGCGCCGACGATCACcg                                                                                                                       >  2:175061/1‑137 (MQ=255)
                       cgggccGGCCCGCCGGAGGTGGCGGGACTGGTCGTTGTTGACGCGGGGCCTGAGGGTCAGGGGCCAGCCGGCCTGCTGCTGTACCATGCCCAGGGGGCGCCAAACTTTGTGGCCCGCGCCGACGATCACCGCGTTCa                                                                                                                 <  8:337394/132‑1 (MQ=255)
                         ggTCGGCACGCCCGACGTGGCCGGCCAGGTCCTTGGTGAAGCGGTGGCAGACGGTCACGGTGCAGCCGGCCAGCAGCAGTTCCATGGCCATCGGGCGGCCAACGATGTTGGACGCGCCGACGATCACCGCGTTCATg                                                                                                               >  7:215240/1‑137 (MQ=255)
                            cGGCACGCCCGACGTGGCCGGCCAGGTCCTTGGTGAAGCGGTGGCAGACGGTCACGGTGCAGCCGGCCAGCAGCAGTTCCATGGCCATCGGGCGGCCAACGATGTTGGACGCGCCGACGATCACCGCGTTCATGCCg                                                                                                            >  7:466929/1‑137 (MQ=255)
                                         ggggggggccGGGTCGTTGGTTAGGGGGGGGCCGCCGGTCCGGGGGCCGCGGCCCCGCAGCAGTCCCAGGCCCCGGGGGGGGCCAACTTTTTGGGCGGCCCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGc                                                                                               <  8:441902/131‑1 (MQ=255)
                                          tGGCCGGCCAGGTCCTTGGTGAAGCGGTGGCAGACGGTCACGGTGCAGCCGGCCAGCAGCAGTTCCATGGCCATCGGGCGGCCAACGATGTTGGACGCGCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGcc                                                                                              >  7:210046/1‑137 (MQ=255)
                                           ggcgggccTGGGCGTTGGTGCAGCGGGGGCCGCCGGCCCGGGGGCCCCCGCCCAGCAGCTGTCCATTGCCAAGCGGGCGGCCAACGAGGTGGCAGGCGCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGCCg                                                                                             <  8:466929/137‑1 (MQ=255)
                                               ggccAGGTCCTTGGTGAAGCGGTGGCAGACGGTCACGGTGCAGCCGGCCAGCAGCAGTTCCATGGCCATCGGGCGGCCAACGATGTTGGACGCGCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGCCGGTGc                                                                                         >  7:135240/1‑137 (MQ=255)
                                                    ggTCCTTGGTCCCGCGGGGGCACACGGCCAGGGGGCCGCCGCCCTGCAGCAGTCCAATGCCCCTGGGGCGGCCAACGTTGTGGGCCGCGCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGCCGGTGCTTTcc                                                                                    <  7:353857/137‑1 (MQ=255)
                                                         ttGGTGAAGCGGTGGCAGACGGTCACGGTGCAGCCGGCCAGCAGCAGTTCCATGGCCATCGGGCGGCCAACGATGTTGGACGCGCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGCCGGTGCTTTCcagcag                                                                               >  2:217166/1‑137 (MQ=255)
                                                              gAAGCGGTGGCAGACGGTCACGGTGCAGCCGGCCAGCAGCAGTTCCATGGCCATCGGGCGGCCAACGATGTTGGACGCGCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGCCGGTGCTTTCCAGCAGGGTCa                                                                          >  5:198444/1‑137 (MQ=255)
                                                                  cGGTGGCAGACGGTCACGGTGCAGCCGGCCAGCAGCAGTTCCATGGCCATCGGGCGGCCAACGATGTTGGACGCGCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGCCGGTGCTTTCCAGCAGGGTCatgat                                                                      >  8:393820/1‑137 (MQ=255)
                                                                     tGGCAGACGGTCACGGTGCAGCCGGCCAGCAGCAGTTCCATGGCCATCGGGCGGCCAACGATGTTGGACGCGCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGCCGGTGCTTTCCAGCAGGGTCATGATGcc                                                                   >  6:276701/1‑137 (MQ=255)
                                                                       ccatcgggTCACGGTGCAGCCGGCCAGCAGCTGTTCCTTGGCCATGGGGCGGCCAACGTTGGTGGCCGCGCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGCCGGTGCTTTCCAGCAGGGTCATGATGCCtt                                                                 <  5:332935/131‑1 (MQ=255)
                                                                                  ggggccAGCCGGCCCGCAGCAGTCCCATGGCCTGGGGCGGCCCAACTATGTGGCCCGCGCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGCCGGTGCTTTCCAGCAGGGTCATGATGCCTTTCGGTGTGCAc                                                      <  4:374169/132‑1 (MQ=255)
                                                                                    gggccgCCGGCCCGCCCAAGTCCCAGGCCCAGCGGGCGCCCAACTTGGGGGGACGCCCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGCCGGTGCTTTCCAGCAGGGTCATGATGCCTTTCGGTGTGCACgg                                                    <  1:359282/132‑1 (MQ=255)
                                                                                       ccgcgggCCAGCAGCTGTTCCAGGGCCTGCGGGCGGCCAAGGTTGTTGGCCGCGCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGCCGGTGCTTTCCAGCAGGGTCATGATGCCTTTCGGTGTGCACGGgcg                                                 <  6:499310/132‑1 (MQ=255)
                                                                                         gccgccCTGCACCAGTTCCTGGGCAAGCGGGCGCCAAAGTATGTTGGCCGCGCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGCCGGTGCTTTCCAGCAGGGTCATGATGCCTTTCGGTGTGCACGGGCgca                                               <  5:169054/137‑1 (MQ=255)
                                                                                         gCCGGCCAGCACCAGTCCCATGCCCAGGGGGCGGCCAACGATGTGGGCCGCGCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGCCGGTGCTTTCCAGCAGGGTCATGATGCCTTTCGGTGTGCACGGGCgca                                               <  5:446913/137‑1 (MQ=255)
                                                                                         gCCGGCCACCCGCTGTCCCCGGGCCCGGGGGGGGCCACCGTTGTGGGCCGCGCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGCCGGTGCTTTCCAGCAGGGTCATGATGCCTTTCGGTGTGCACGGGCgca                                               <  3:351965/137‑1 (MQ=255)
                                                                                         cccGCCCAGCCGCAGTCCAATGCCCTGCGGGCGGCCAACGATGTTGCCCGCGCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGCCGGTGCTTTCCAGCAGGGTCATGATGCCTTTCGGTGTGCACGGGCgca                                               <  5:99221/136‑1 (MQ=255)
                                                                                          ccGGCCAGCAGCAGTTCCATGGCCATCGGGCGGCCAACGATGTTGGACGCGCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGCCGGTGCTTTCCAGCAGGGGCATGATGCCTTTCGGTGTGCACGGGCgcag                                              >  8:155372/1‑137 (MQ=255)
                                                                                            ggCCAGCAGCAGTTCCATGGCCATCGGGCGGCCAACGATGTTGGACGCGCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGCCGGTGCTTTCCAGCAGGGTCATGATGCCTTTCGGTGTGCACGGGCgcagca                                            >  5:366172/1‑137 (MQ=255)
                                                                                                  cagcagTTCCATGGCCATCGGGCGGCCAACGATGTTGGACGCGCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGCCGGTGCTTTCCAGCAGGGTCATGATGCCTTTCGGTGTGCACGGGCGCAGCAGTGGaa                                      >  8:314753/1‑137 (MQ=255)
                                                                                                   tgcagTTCCATGGCCTTGGGGGGGCCAATGTTGTTGGCCGCGCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGCCGGTGCTTTCCAGCAGGGTCATGATGCCTTTCGGTGTGCACGGGCGCAGCAGTGGAAt                                     <  5:385318/136‑1 (MQ=255)
                                                                                                    cccattCCATGGCCTAGGGGGCGCCAAAGTTTGTTGCCCGCGCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGCCGGTGCTTTCCAGCAGGGTCATGATGCCTTTCGGTGTGCACGGGCGCAGCAGTGGAAt                                     <  1:131724/133‑1 (MQ=255)
                                                                                                     cagTTCCATGGCCATCGGGCGGCCAACGATGTTGGACGCGCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGCCGGTGCTTTCCAGCAGGGTCATGATGCCTTTCGGTGTGCACGGGCGCAGCAGTGGAATAc                                   >  5:402870/1‑137 (MQ=255)
                                                                                                                ccATCGGGCGGCCAACGATGTTGGACGCGCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGCCGGTGCTTTCCAGCAGGGTCATGATGCCTTTCGGTGTGCACGGGCGCAGCAGTGGAATACGCTGGGCCAgg                        >  6:206199/1‑137 (MQ=255)
                                                                                                                ccATCGGGCGGCCAACGATGTTGGACGCGCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGCCGGTGCTTTCCAGCAGGGTCATGATGCCTTTCGGTGTGCACGGGCGCAGCAGTGGAATACGCTGGGCCAgg                        >  3:52500/1‑137 (MQ=255)
                                                                                                                ccATCGGGCGGCCAACGATGTTGGACGCGCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGCCGGTGCTTTCCAGCAGGGTCATGATGCCTTTCGGTGTGCACGGGCGCAGCAGTGGAATACGCTGGGCCAgg                        >  2:162249/1‑137 (MQ=255)
                                                                                                                  atgcGGGCGGCAAACGTTGTGGCCCGCCCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGCCGGTGCTTTCCAGCAGGGTCATGATGCCTTTCGGTGTGCACGGGCGCAGCAGTGGAATACGCTGGGCCAggc                       <  1:32573/134‑1 (MQ=255)
                                                                                                                        gcggcCAACGATGTTGGACGCGCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGCCGGTGCTTTCCAGCAGGGTCATGATGCCTTTCGGTGTGCACGGGCGCAGCAGTGGAATACGCTGGGCCAGGCGGCCGa                 >  5:134808/1‑137 (MQ=255)
                                                                                                                               taCGATGATGGACGCGCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGCCGGTGCTTACCAGCAGCGTCATGATGCGATTCCGTGTCCACGGGCGCAGCAGTGTAATACGCTGGGCCAGACGCCCGAtgatct           >  2:371557/2‑133 (MQ=255)
                                                                                                                                   aTGTTGGACGCGCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGCCGGTGCTTTCCAGCAGGGTAATGATGCCTTTCGGTGTGCACGGGCGCAGCAGTGGAATACGCTGGGCCAGGCGGCCGATGTTGTACgg       >  6:315034/1‑137 (MQ=255)
                                                                                                                                        gggACGCGCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGCCGGTGCTTTCCAGCAGGGTCATGATGCCTTTCGGTGTGCACGGGCGCAGCAGTGGAATACGCTGGGCCAGGCGGCCGATGTTGTACGGGTgg   <  1:66058/136‑1 (MQ=255)
                                                                                                                                         ggACGCGCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGCCGGTGCTTTCCAGCAGGGTCATGATGCCTTTCGGTGTGCACGGGCGCAGCAGTGGAATACGCTGGGCCAGGCGGCCGATGTTGTACGGGTGGa  >  7:265873/1‑137 (MQ=255)
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GGCTTGCCGGCAGCCACGACCACCAGGTCGGCACGCCCGACGTGGCCGGCCAGGTCCTTGGTGAAGCGGTGGCAGACGGTCACGGTGCAGCCGGCCAGCAGCAGTTCCATGGCCATCGGGCGGCCAACGATGTTGGACGCGCCGACGATCACCGCGTTCATGCCGTACAGGTCCTGGCCGGTGCTTTCCAGCAGGGTCATGATGCCTTTCGGTGTGCACGGGCGCAGCAGTGGAATACGCTGGGCCAGGCGGCCGATGTTGTACGGGTGGA  >  KT2440/2577914‑2578184

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: