Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,440,3430AC54.5% ‑6.4 / 15.8 44S18R (AGC→CGC) CJ019_03266hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (14/6);  new base C (19/5);  total (33/11)
Fisher's exact test for biased strand distribution p-value = 5.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.12e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CGATCACCAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAA  >  KT2440/3440210‑3440473
                                                                                                                                     |                                                                                                                                  
cGATCACCAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAgcc                                                                                                                                 >  6:316159/1‑136 (MQ=255)
                 aaTGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCCCTCGCGGCGCCCGGGcc                                                                                                                >  5:383561/1‑136 (MQ=255)
                 aaTGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGcc                                                                                                                >  6:473591/1‑136 (MQ=255)
                          aGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCGCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCGGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCCCTGGCGGCGCCCGGGCAGCCTGGgtg                                                                                                       <  4:484395/137‑1 (MQ=255)
                           gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCGGGgtgt                                                                                                      >  1:337141/1‑137 (MQ=255)
                           gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCACACCTCGCCGCGCCCGGGCCGCCGGGgggt                                                                                                      >  3:59088/1‑137 (MQ=255)
                                              cAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCGATaa                                                                                   >  7:467196/1‑137 (MQ=255)
                                               aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCCGGCAGCCGGGGTGTGGGAGGCCCACCCCTaaaaa                                                                                  >  4:316845/1‑136 (MQ=255)
                                               aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCGGGCGGCGCCCGGGCCGCCGGGGTGTGGCCGGCCAACGCCTATAAg                                                                                  >  5:192695/1‑137 (MQ=255)
                                                 ggCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCCGCCTGGGTGTGGCAGGCCAACGCCTATAAGgc                                                                                >  7:486153/1‑137 (MQ=255)
                                                 acaccTCTACCCGCGCGCGGGCGTGGGGCGGCTGTGCGCGGTGGGGCCCCCCGGGTGGGCGCGGTGGCGCGGCGGCGGCCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGgc                                                                                <  8:17350/134‑1 (MQ=255)
                                                      acaacacGCGAGCGCTCGCGGTGCGGCCGGGAGCGCTGGGGCCACCCGCCGGGGCGCCGTGGCGCTGCGGCGGGCCGCCAGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGc                                                                           <  8:64754/134‑1 (MQ=255)
                                                         aCAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCCGCCCGGGCGGCGCCCGGGCCCCCCGGGGGTGGCAGGCCAACGCCTATAATGCGCAGCTca                                                                        >  2:126461/1‑136 (MQ=255)
                                                           ccccgcgcgcTCGTGGGGCGGCAGGGCGCGCGGGCCCCCCCCGCGGGGGCGCGGGGGGGCGGCGGCGGGCCGCCCGCGGGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCt                                                                      <  4:479378/131‑1 (MQ=255)
                                                            cgcgcgcgcTCGCGGTGCTGCCGTGAGCGCTGGGGCCACCCGCCTGGGCGCCGTGGCGCTGCTGCGGGCCGCCAGCTCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTg                                                                     <  8:467196/136‑1 (MQ=255)
                                                                agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGGAGGCCAACTCCTATAAGGCGCCGCTCGCTGATCa                                                                 >  5:221577/1‑137 (MQ=255)
                                                                agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCTGGCGGCGCCCGGGCAGCCGGGGTGTTGCAGGCCCACCCCAATAAGGCGCAGCTCGCTGAtaa                                                                 >  7:432047/1‑135 (MQ=255)
                                                                   gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCCGGCCCCCTGGGGGGGGCAGGCCAACCCCGATAAGGCGCACCTCGCTGATCAGGc                                                              >  2:275933/1‑137 (MQ=255)
                                                                   gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCCGGCAGCCGGGGGGTGGCAGGCCCACGCCTATAAGGCGCAGCGCGCTGATCAGGc                                                              >  1:53345/1‑137 (MQ=255)
                                                                   gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGCCGGGCAACGCCGATAAGGAGCAGCTCGCTGATCAGGc                                                              >  5:149649/1‑137 (MQ=255)
                                                                   gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATcctgt                                                              >  7:17350/1‑133 (MQ=255)
                                                                   gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCGGGCGGCGCCCGGGCAGGCCGGGTGTGGCAGGCCAACCCCAATAAGGAGCAGGTCGCAGATCAGGc                                                              >  1:410985/1‑137 (MQ=255)
                                                                   gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCAGGCGGCGCCCCGGCAGCCAGGGGGTGGCCGGCCAACGCCTATAATGCGCAGCTAGCTGATCACGc                                                              >  2:269643/1‑137 (MQ=255)
                                                                     tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCCGGCCCACGCCGATAAAGGGCAGCTCGCGGATCAGGccc                                                            >  2:429021/1‑136 (MQ=255)
                                                                     tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCTGGGGGTGGCAGGCCAACGCCCATAAGGCGCACCTCGCGGATCAGGCCg                                                            >  8:57781/1‑137 (MQ=255)
                                                                     tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCAGGCGGCGCCCCGGCCGCCTGGGTGTGGCAGGCCACCCCCAATAAGGCGCAGCGCGCTGCTAAGGCCg                                                            >  3:367878/1‑137 (MQ=255)
                                                                     tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCCGGCCGCCGGGGTGTGGGCGGCCAACCCCGATAAGGCGCAGCTCGCTGATCAGGccc                                                            >  2:210622/1‑136 (MQ=255)
                                                                     tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCGGCTCAGGGCg                                                            >  6:195718/1‑137 (MQ=255)
                                                                     tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCACCGCGGGCGGCGCCCCCGCCGCCGGGGGGTGGGAGGCCCACCCCGATAAGGCGCAGCGCGCGGATCAAGccc                                                            >  4:190220/1‑136 (MQ=255)
                                                                     tCGAGGTGCTGCAGTGAGCGCTTGGGCCACACGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCACCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCCGGCCAACGCCGTAAAGGCGCAGCTCGCGGATCAGGCCg                                                            >  8:166220/1‑137 (MQ=255)
                                                                                cAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCCCTGGCGGCGCCCGGGCCGCCGGGGGGTGGGCGGCCCACCCCGCTAATGCGCCGCTCGCTGATCAGGCCGAGGACTTCGGc                                                 >  8:24767/1‑137 (MQ=255)
                                                                                 tgTGAGCGGGGGGGCCACGCGGGTGGGCGGCGGGGCGGGGCGGCGGCCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCa                                                <  7:310416/136‑1 (MQ=255)
                                                                                        gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCTGGGGGTGGCAGGCCCACGCCGATAAGGCGCAGCTCGCGGCTCAGGCCGATGCCTTCGGCAAGCAGtt                                         >  7:169656/1‑137 (MQ=255)
                                                                                        gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGGCGGCCAACCCCTATAATGCGCAGCTCGCTGATCAGGCCGCTGACTTCGGCCAGCAGtt                                         >  8:353593/1‑137 (MQ=255)
                                                                                        gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGCTGACTTCGGCCAGCAGtt                                         >  6:408971/1‑137 (MQ=255)
                                                                                        gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCCGGCCACCGCCGATAAGGCGCAGCTCGCGGGTAAGGCCGCTGACTTCGGCAAGCAGtt                                         >  4:430440/1‑137 (MQ=255)
                                                                                         ctggggCCACCCGGCTGGGCGGGGGGGCGCTGCGGCGGGCCGCCAGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTg                                        <  7:29464/134‑1 (MQ=255)
                                                                                          ttGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCCGGCAGCCTGGGTGTGGGAGGCCAACGCCGATAAGGCGCCGCTCGCGGATCAGGCCGAGGACTTCGGGCAGCAGTTgg                                       >  6:386302/1‑137 (MQ=255)
                                                                                                cACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCTGGGGGTGGGAGGCCCACGCCGATAAGGCGCAGCTCGCGGATCAGGCCGCGGACTTCGGCCAGCAGTTGGCGgtga                                 >  7:315666/1‑137 (MQ=255)
                                                                                                     gCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCCCGCGGGCGGCGGCCCGGCCGCCGGGGTGGGGGCGGCCACCCCCGATAATGCGCAGCTCGCGGATGACGCCCGTGACTTAGGGCAGCAGTTGGGGGGGGAGGAt                            >  6:328189/1‑137 (MQ=255)
                                                                                                          ggggCCGGGGCGGTGCGGCGGGCCGCCAGCGCGGGGGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc                       <  7:267814/134‑1 (MQ=255)
                                                                                                          ggcgcgGTGGCGCTGCTGCGGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc                       <  5:424517/135‑1 (MQ=255)
                                                                                                                    ggctgctgggTGCCGCCAGCGGGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTg             <  7:181600/136‑1 (MQ=255)
                                                                                                                               gccgccCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGaa  <  5:487310/137‑1 (MQ=255)
                                                                                                                                     |                                                                                                                                  
CGATCACCAAACGTATCAATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAA  >  KT2440/3440210‑3440473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑