Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,440,3480TG46.3% 3.4 / 15.2 43A19A (GCT→GCG) CJ019_03266hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (20/2);  new base G (10/9);  total (32/11)
Fisher's exact test for biased strand distribution p-value = 1.15e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.47e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAA  >  KT2440/3440227‑3440473
                                                                                                                         |                                                                                                                             
aaTGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCCCTCGCGGCGCCCGGGcc                                                                                                                >  5:383561/1‑136 (MQ=255)
aaTGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGcc                                                                                                                >  6:473591/1‑136 (MQ=255)
         aGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCGCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCGGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCCCTGGCGGCGCCCGGGCAGCCTGGgtg                                                                                                       <  4:484395/137‑1 (MQ=255)
          gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCGGGgtgt                                                                                                      >  1:337141/1‑137 (MQ=255)
          gCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCACACCTCGCCGCGCCCGGGCCGCCGGGgggt                                                                                                      >  3:59088/1‑137 (MQ=255)
                             cAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCGATaa                                                                                   >  7:467196/1‑137 (MQ=255)
                              aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCCGGCAGCCGGGGTGTGGGAGGCCCACCCCTaaaaa                                                                                  >  4:316845/1‑136 (MQ=255)
                              aaGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCGGGCGGCGCCCGGGCCGCCGGGGTGTGGCCGGCCAACGCCTATAAg                                                                                  >  5:192695/1‑137 (MQ=255)
                                ggCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCCGCCTGGGTGTGGCAGGCCAACGCCTATAAGgc                                                                                >  7:486153/1‑137 (MQ=255)
                                acaccTCTACCCGCGCGCGGGCGTGGGGCGGCTGTGCGCGGTGGGGCCCCCCGGGTGGGCGCGGTGGCGCGGCGGCGGCCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGgc                                                                                <  8:17350/134‑1 (MQ=255)
                                     acaacacGCGAGCGCTCGCGGTGCGGCCGGGAGCGCTGGGGCCACCCGCCGGGGCGCCGTGGCGCTGCGGCGGGCCGCCAGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGc                                                                           <  8:64754/134‑1 (MQ=255)
                                        aCAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCCGCCCGGGCGGCGCCCGGGCCCCCCGGGGGTGGCAGGCCAACGCCTATAATGCGCAGCTca                                                                        >  2:126461/1‑136 (MQ=255)
                                          ccccgcgcgcTCGTGGGGCGGCAGGGCGCGCGGGCCCCCCCCGCGGGGGCGCGGGGGGGCGGCGGCGGGCCGCCCGCGGGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCt                                                                      <  4:479378/131‑1 (MQ=255)
                                           cgcgcgcgcTCGCGGTGCTGCCGTGAGCGCTGGGGCCACCCGCCTGGGCGCCGTGGCGCTGCTGCGGGCCGCCAGCTCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTg                                                                     <  8:467196/136‑1 (MQ=255)
                                               agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGGAGGCCAACTCCTATAAGGCGCCGCTCGCTGATCa                                                                 >  5:221577/1‑137 (MQ=255)
                                               agcgCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCTGGCGGCGCCCGGGCAGCCGGGGTGTTGCAGGCCCACCCCAATAAGGCGCAGCTCGCTGAtaa                                                                 >  7:432047/1‑135 (MQ=255)
                                                  gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCCGGCCCCCTGGGGGGGGCAGGCCAACCCCGATAAGGCGCACCTCGCTGATCAGGc                                                              >  2:275933/1‑137 (MQ=255)
                                                  gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCCGGCAGCCGGGGGGTGGCAGGCCCACGCCTATAAGGCGCAGCGCGCTGATCAGGc                                                              >  1:53345/1‑137 (MQ=255)
                                                  gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGCCGGGCAACGCCGATAAGGAGCAGCTCGCTGATCAGGc                                                              >  5:149649/1‑137 (MQ=255)
                                                  gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATcctgt                                                              >  7:17350/1‑133 (MQ=255)
                                                  gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCGGGCGGCGCCCGGGCAGGCCGGGTGTGGCAGGCCAACCCCAATAAGGAGCAGGTCGCAGATCAGGc                                                              >  1:410985/1‑137 (MQ=255)
                                                  gcTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCAGGCGGCGCCCCGGCAGCCAGGGGGTGGCCGGCCAACGCCTATAATGCGCAGCTAGCTGATCACGc                                                              >  2:269643/1‑137 (MQ=255)
                                                    tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCCGGCCCACGCCGATAAAGGGCAGCTCGCGGATCAGGccc                                                            >  2:429021/1‑136 (MQ=255)
                                                    tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCAGCCTGGGGGTGGCAGGCCAACGCCCATAAGGCGCACCTCGCGGATCAGGCCg                                                            >  8:57781/1‑137 (MQ=255)
                                                    tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCAGGCGGCGCCCCGGCCGCCTGGGTGTGGCAGGCCACCCCCAATAAGGCGCAGCGCGCTGCTAAGGCCg                                                            >  3:367878/1‑137 (MQ=255)
                                                    tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCCGGCCGCCGGGGTGTGGGCGGCCAACCCCGATAAGGCGCAGCTCGCTGATCAGGccc                                                            >  2:210622/1‑136 (MQ=255)
                                                    tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCGGCTCAGGGCg                                                            >  6:195718/1‑137 (MQ=255)
                                                    tCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCACCGCGGGCGGCGCCCCCGCCGCCGGGGGGTGGGAGGCCCACCCCGATAAGGCGCAGCGCGCGGATCAAGccc                                                            >  4:190220/1‑136 (MQ=255)
                                                    tCGAGGTGCTGCAGTGAGCGCTTGGGCCACACGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCACCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCCGGCCAACGCCGTAAAGGCGCAGCTCGCGGATCAGGCCg                                                            >  8:166220/1‑137 (MQ=255)
                                                               cAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCCCTGGCGGCGCCCGGGCCGCCGGGGGGTGGGCGGCCCACCCCGCTAATGCGCCGCTCGCTGATCAGGCCGAGGACTTCGGc                                                 >  8:24767/1‑137 (MQ=255)
                                                                tgTGAGCGGGGGGGCCACGCGGGTGGGCGGCGGGGCGGGGCGGCGGCCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCa                                                <  7:310416/136‑1 (MQ=255)
                                                                       gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCTGGGGGTGGCAGGCCCACGCCGATAAGGCGCAGCTCGCGGCTCAGGCCGATGCCTTCGGCAAGCAGtt                                         >  7:169656/1‑137 (MQ=255)
                                                                       gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCTGGCGGCGCCCGGGCCGCCGGGGTGTGGGCGGCCAACCCCTATAATGCGCAGCTCGCTGATCAGGCCGCTGACTTCGGCCAGCAGtt                                         >  8:353593/1‑137 (MQ=255)
                                                                       gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCCCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGCTGACTTCGGCCAGCAGtt                                         >  6:408971/1‑137 (MQ=255)
                                                                       gcTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCCCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCCGGCCACCGCCGATAAGGCGCAGCTCGCGGGTAAGGCCGCTGACTTCGGCAAGCAGtt                                         >  4:430440/1‑137 (MQ=255)
                                                                        ctggggCCACCCGGCTGGGCGGGGGGGCGCTGCGGCGGGCCGCCAGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTg                                        <  7:29464/134‑1 (MQ=255)
                                                                         ttGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCCGGCAGCCTGGGTGTGGGAGGCCAACGCCGATAAGGCGCCGCTCGCGGATCAGGCCGAGGACTTCGGGCAGCAGTTgg                                       >  6:386302/1‑137 (MQ=255)
                                                                               cACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCCGCGCGGGCGGCGCCCGGGCCGCCTGGGGGTGGGAGGCCCACGCCGATAAGGCGCAGCTCGCGGATCAGGCCGCGGACTTCGGCCAGCAGTTGGCGgtga                                 >  7:315666/1‑137 (MQ=255)
                                                                                    gCCTGGTCGCCGTGGCGCTGCTGCTGGGCGCCCCCGCGGGCGGCGGCCCGGCCGCCGGGGTGGGGGCGGCCACCCCCGATAATGCGCAGCTCGCGGATGACGCCCGTGACTTAGGGCAGCAGTTGGGGGGGGAGGAt                            >  6:328189/1‑137 (MQ=255)
                                                                                         ggggCCGGGGCGGTGCGGCGGGCCGCCAGCGCGGGGGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc                       <  7:267814/134‑1 (MQ=255)
                                                                                         ggcgcgGTGGCGCTGCTGCGGGCCGCCCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATcgcgc                       <  5:424517/135‑1 (MQ=255)
                                                                                                   ggctgctgggTGCCGCCAGCGGGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTg             <  7:181600/136‑1 (MQ=255)
                                                                                                              gccgccCGCGCGGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGaa  <  5:487310/137‑1 (MQ=255)
                                                                                                                         |                                                                                                                             
AATGGTGGTAGCAACGGTCTGGAGGATCGCAAGGCCCTCTACAAGCGAGCGCTCGAGGTGCTGCAGTGAGCGCTTGGGCCACCCGCCTGGTCGCCGTGGCGCTGCTGCTGGCCGCCAGCGCTGGCGGCGCCCGGGCAGCCTGGGTGTGGCAGGCCAACGCCTATAAGGCGCAGCTCGCTGATCAGGCCGATGACTTCGGCAAGCAGTTGGCGGAGAAGGATCGCGCGCACGGTCTGGAGCGGGAGAA  >  KT2440/3440227‑3440473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑