Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,586,4080CA38.2% 72.9 / 10.5 58F129L (TTC→TTA) CJ019_03399hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (13/21);  new base A (21/0);  total (36/22)
Fisher's exact test for biased strand distribution p-value = 1.44e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GTGGTGGCCGAACAGTTTGGCACCCTCAATACATTGCACCCAGGTCGTATCGACCTGGGCCTGGGCCGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCAGAGCGGGATTTCGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGATGTGCCGGTGTGGATCCTCGGTTCCAGCCTGTTCGGTGCTGACCTGGCGGCGA  >  KT2440/3586277‑3586541
                                                                                                                                   |                                                                                                                                     
gtggtggCCGAACAGTTTGGCACCCTCAATACATTGCACCCAGGTCGTATCGACCTGGGCCTGGGCCGTGCGCCAGGCACGTCCGGGCCGCCCGTTCGGGCATTGCGCGGCGCCGCGCCCCCGCGGGCTTTAgggcc                                                                                                                                  >  1:157051/1‑134 (MQ=255)
  ggtggCCGAACAGTTTGGCACCCTCAATACATTGCACCCAGGTCGTATCGACCTGGGCCTGGGCCGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCAGAGCGGGATTTCGGCCAgg                                                                                                                                <  8:94325/137‑1 (MQ=255)
        cGAACAGTTTGGCACCCTCAATACATTGCACCCAGGTCGTATCGACCTGGGCCTGGGCCGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCAGAGCGGGATTTCGGCCAGGACATCg                                                                                                                          >  2:131451/1‑137 (MQ=255)
            cattttGGCACCCTCAATACATTGCCCCCTGGGCGTATCGACCTGGGCGGGGGCCGCGCGCCAGGCACGGCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCAGAGCGGGATTTCGGCCAGGACATCGCCGa                                                                                                                      <  1:351048/134‑1 (MQ=255)
                           aaTACATTGCACCCAGGTCGTATCGACCTGGGCCTGGGCCGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGGGGGGCGCCCCGCCCGAGCGGGCTTTAGGCCAGGTCATCGCCCCGCTGGGTGGTTACCt                                                                                                       >  5:370691/1‑137 (MQ=255)
                            aTACATTGCACCCAGGTCGTATCGACCTGGGCCTGGGCCGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCAGAGCGGGATTTAGGCCAGGACATCGCCGAGCTGCGTGATTACCtt                                                                                                      >  6:110021/1‑136 (MQ=255)
                            aTACATTGCACCCAGGTCGTATCGACCTGGGCCTGGGCCGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCAGAGCGGGAATTAGGGCACGACATCGCCGACCTGCGTTATTAccag                                                                                                      >  3:151135/1‑135 (MQ=255)
                                    cACCCAGGTCGTATCGACCTGGGCCTGGGCCGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCAGCGCGGGATTTCGGCCAGGACATCGCCGCGCGGCGGGATTACCTGGCCGACaa                                                                                              >  4:320980/1‑137 (MQ=255)
                                    cACCCAGGTCGTATCGACCTGGGCCTGGGCCGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCCCCCCCCCACAGCGGGATTTAGGCCAGGACATAGCCGAGCTGCGTGATTAACTGGCCGCCaa                                                                                              >  3:221939/1‑137 (MQ=255)
                                     aCCCAGGTCGTATCGACCTGGGCCTGGGCCGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCCGCGCGGGCTTTAGGCCAGGCCATCGCCGAGCTGGGGGATTACCTGGCCGCCAAc                                                                                             >  7:480775/1‑137 (MQ=255)
                                     aCCCAGGTCGTATCGACCTGGGCCTGGGCCGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCCGAGCGGGGTTTGGGGCCGGGCATCGCCGCGCGGGGGGGTTTACTGGCCGCCAAc                                                                                             >  3:91534/1‑137 (MQ=255)
                                     aCCCAGGTCGTATCGACCTGGGCCTGGGCCGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCCGAGCGGGATTTCGGCCAGGCCATCGCCGCGCTGCGGGATTACCTGGCCGCCAAc                                                                                             >  5:378373/1‑137 (MQ=255)
                                     aCCCAGGTCGTATCGACCTGGGCCTGGGCCGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCAGAGCGGGATTTAGGCCCGGACATCGCCGAGCTGCGTGATTAACTGGCCGCCAAc                                                                                             >  8:403613/1‑137 (MQ=255)
                                     aCCCAGGTCGTATCGACCTGGGCCTGGGCCGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCCCGCCCGCGCGGGGTTTATGCCCGGGCCTCGCCGCGCGGCGTGATTAACAGGGCGCCaaa                                                                                             >  3:17386/1‑136 (MQ=255)
                                     aCCCAGGTCGTATCGACCTGGGCCTGGGCCGTGCGCCAGACACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCAGAGCGGGATTTAGGCCAGGCCCTCGCCGCGCGGGGGGATTACCAGGCCGACAAc                                                                                             >  2:463276/1‑137 (MQ=255)
                                      cccAGGTCGTATCGACCTGGGCCTGGGCCGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCCGCGCGGGGTTTAGGCCAGGACCTAGCCGCGCTGCGTGGTTACCTGGCCGCCAACg                                                                                            >  3:386101/1‑137 (MQ=255)
                                      cccAGGTCGTATCGACCTGGGCCTGGGCCGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCAGAGCGGGATTTAGGCCAGGCCATCGCCGCGCTGCGTGATTACCTGGCCGCCAAcc                                                                                            >  8:104429/1‑136 (MQ=255)
                                      cccAGGTCGTAGCCACCTGGGCCTGGGCCGGGCGCCAGGCACGTCCGGGCCGACCGTGCGGGCTTTGCGCGGCGCCGCGCCAGAGCGGGATTTCGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACg                                                                                            <  2:81185/137‑1 (MQ=255)
                                            tCGTATCGACCTGGGCCTGGGCCGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCAGAGCGGGGTTTCGGCCAGGCCATCGCCGCGCGGCGGGATTACCTGGCCGCCAACGGCAAAc                                                                                      >  4:66040/1‑137 (MQ=255)
                                            tCGTATCGACCTGGGCCTGGGCCGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCCCGCCCGCGCGGGGTTTAGGCCCGGCCAACGCCGCGCGGCGGGATTTCCAGGGCGCCAAACGCaaaa                                                                                      >  4:43749/1‑136 (MQ=255)
                                                   gACCTGGGCCTGGGCCGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGGGCGGCCCCCCGCCCGCCCCGGCTTTAGGCCCGGACCTAGCCGAGCGGCGTGATTTACTGGCCGACAACCGCAAAACCCgggg                                                                               >  1:167242/1‑136 (MQ=255)
                                                       tgggcctgggccGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCAGAGCGGGATTTAGGCCCGGACATGGCCGAGCTGCGGGATTACCTTGCCGACAACCGCCAAAGCCCGGTgcgc                                                                           >  8:111052/1‑137 (MQ=255)
                                                        gggcctgggccGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCAGAGCGGGATTTCGGCCAGGACATCGCCGCGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGcgcg                                                                          >  6:244662/1‑137 (MQ=255)
                                                           cctgggccGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCAGAGCGGGATTTCGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCgcggcg                                                                       >  8:206448/1‑137 (MQ=255)
                                                            ctgggccGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCAGCGCGGGATTTAGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGt                                                                      >  6:461210/1‑137 (MQ=255)
                                                            ctgggccGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCAGAGCGGGATTTCGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGt                                                                      >  4:381679/1‑137 (MQ=255)
                                                            ctgggccGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCAGAGCGGGATTTAGGCCCGGGCATCGCCGAGCTGCGGGCTTACCTGGCCGCCCACGGCAAACCCCCGGTGCgcggcgg                                                                      >  2:109954/1‑136 (MQ=255)
                                                                  cGTGCGCCAGGCACGTCCGGGCCGCCCGTTCGGGCAATGCCCCGCGCCGCGCCAGCGCGGGATTTCCGCCCGGACCTCGCCGAGCTGCGTGATTACCAGGCCGCCAACGGCCAACGCCCGGTGCGCGGGCTTCCggg                                                                >  8:389396/1‑137 (MQ=255)
                                                                  cGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCAGAGCGGGCTTTAGGCCAGGACATAGCCGCGCTGGGGGGTTAACTTGGCGACAACGGCAAACGCCGGGTGAGCGGCGTACCggg                                                                >  1:312055/1‑137 (MQ=255)
                                                                  cGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCCCCCGGGCGGGGTTTAGGCCCGGCCAAAGCCGCGCGGGGGGATTTACAGGCCGCCAACCGCAAACCCCCGGTGCGCGGCGGACCggg                                                                >  2:192925/1‑137 (MQ=255)
                                                                   gggcgcCTGGCACGGCGGGGCCGACCGTGCGGGCATGGCGCGGCGCCGCGCCAGAGCGGGATTTCGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGt                                                               <  7:197162/135‑1 (MQ=255)
                                                                       gcCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCAGCGCGGGATTTTGGCCCGGACAACGCCCCGCTGCGTGTTTACCAGGCCGCCAACGGCCAACGCCCGGGGCGCGGCGTTCCGGGGgcgg                                                           >  1:394066/1‑136 (MQ=255)
                                                                       gcCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCAGAGCGGGATTTCGGCCCGGACATCGCCGAGCTGCGTGATTACCTGGGCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTgcgc                                                           >  8:288152/1‑137 (MQ=255)
                                                                       gcCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCCCCAGCGCGGGATTTCGGCCAGGCCATCGCCGAGCTGAGTGATTACCTGGCCGTCAACGGCAAACGCCCGGTGCGCGGCGGACCGGGTgcgc                                                           >  2:143367/1‑137 (MQ=255)
                                                                            gCAGGTCGGGGCCGCCCGTTCGGGCTTGGCGCGGCGCCGCGCCAGAGCGGGATTTCGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGAt                                                      <  7:17837/137‑1 (MQ=255)
                                                                             caggcccGGGCCGACCTTGGGGGATTTGCGCGGCCCCGCGCCAGAGCGGGATTTCGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGAtg                                                     <  1:360307/132‑1 (MQ=255)
                                                                             cACGTCGGGCCCGCCGGGGCGGGCAGCGCGGGCGGCCGCGCCAGCGGGGGATTTCGGCCAGGACATCGCCGAGCTGCGTGATAACCTGGCCGACAACGCCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGAtg                                                     <  1:205765/137‑1 (MQ=255)
                                                                             cACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCAGAGCGGGATTTCGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGAtg                                                     <  6:118119/137‑1 (MQ=255)
                                                                               gggccGGGCCGACCGTGCGGGCATGGCGCGGCGCCGCGCCAGAGCGGGATTTCGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGAtgtg                                                   <  2:352984/134‑1 (MQ=255)
                                                                                        cGACCGTTCGGGCATTGCGCGGCGCCGCGCCAGAGCGGGATTTAGGCCAGGACATAGCCGCGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGATGTGCCGGTGTgg                                          >  3:29873/1‑137 (MQ=255)
                                                                                          cccGTGCGGGCTTTGCGCGGCGCCGCGCCAGAGCGGGATTTTGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGATGTGCCGGTGTGGAt                                        <  2:394066/136‑1 (MQ=255)
                                                                                          acggtgcGGGCATTGCGCGGCGCCGCGCCAGAGCGGGATTTCGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGATGTGCCGGTGTGGAt                                        <  2:420700/131‑1 (MQ=255)
                                                                                          acggTTGGGCCATTGGGGGGCGCCGCGCCAGCGCGGGTTTTCGGCATGGACATCGCCTAGCTGCTTGATTACCTGGCCTACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGATGTGCCGGTGTGGAt                                        <  6:330723/134‑1 (MQ=255)
                                                                                          aCCGTGCGGGCATTGCGCGGCGCCGCGCCAGAGCGGGATTTCGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGATGTGCCGGTGTGGAt                                        <  2:142426/137‑1 (MQ=255)
                                                                                             gTTCGGGCATTGCGCGGCGCCGCGCCAGAGCGGGATTTAGGCCCGGACATCGCCGAGCTGCGGGATTACCTGGCCGCCAACGGCAAAAGCCGGGTGCGCGGCGTACCGGGTGCGCACGATGTGCCGGTGTGGATCCt                                     >  4:170575/1‑137 (MQ=255)
                                                                                             gTTCGGGCATTGCGCGGCGCCGCGCCAGAGCGGGATTTAGGCCACGACATAGCCGAGCTGCGTGATTACCTGGCCGCCAACGGCAAACCCCGGGTGCGCGGCGTACCGGGTGCGCCCGATGTGCCGGTGTGGATccg                                     >  6:96550/1‑136 (MQ=255)
                                                                                               tCGGGATTTGCGGGGCGCCGCGCCAGAGCGGGATTTCGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGATGTGCCGGTGTGGATCCTCg                                   <  7:403613/137‑1 (MQ=255)
                                                                                                    cATTGCGCGGGGCCGCGCCAGAGCGGGAGTTCGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGATGTGCCGGTGTGGATCCTCGGTTcc                              <  8:480775/137‑1 (MQ=255)
                                                                                                    atttGCGCGGCGCCGCGCCAGAGCGGGATTTCGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGATGTGCCGGTGTGGATCCTCGGTTcc                              <  4:91534/135‑1 (MQ=255)
                                                                                                    aaTTGCGCGGCGCCGCGCCAGAGCGGGATTTCGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGATGTGCCGGTGTGGATCCTCGGTTcc                              <  4:17386/136‑1 (MQ=255)
                                                                                                         cggggcgCCGCGCCAGAGCGGGATTTCGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGATGTGCCGGTGTGGATCCTCGGTTCCAGCCt                         <  3:394016/134‑1 (MQ=255)
                                                                                                         cggggcgCCGCGCCAGAGCGGGATTTCGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGATGTGCCGGTGTGGATCCTCGGTTCCAGCCt                         <  6:30612/134‑1 (MQ=255)
                                                                                                              cgccgcgccAGAGCGGGATTTCGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGATGTGCCGGTGTGGATCCTCGGTTCCAGCCTGTTCg                    <  8:478730/137‑1 (MQ=255)
                                                                                                                      cAGAGCGGGATTTCGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGATGTGCCGGTGTGGATCATCGGTTCCAGCATGTTCGGTGCTGAc            >  1:94368/1‑137 (MQ=255)
                                                                                                                                tttCGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGATGTGCCGGTGTGGATCCTCGGTTCCAGCCTGTTCGGTGCTGACCTGGCGGCGa  <  6:227920/137‑1 (MQ=255)
                                                                                                                                tttCGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGATGTGCCGGTGTGGATCCTCGGTTCCAGCCTGTTCGGTGCTGACCTGGCGGCGa  >  4:318916/1‑137 (MQ=255)
                                                                                                                                 ttCGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGATGTGCCGGTGTGGATCCTCGGTTCCAGCCTGTTCGGTGCTGACCTggc       >  2:220283/1‑131 (MQ=255)
                                                                                                                                 ttCGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGATGTGCCGGTGTGGATCCTCGGTTCCAGCCTGTTCGGTGCTGACCTggc       <  1:220283/131‑1 (MQ=255)
                                                                                                                                   |                                                                                                                                     
GTGGTGGCCGAACAGTTTGGCACCCTCAATACATTGCACCCAGGTCGTATCGACCTGGGCCTGGGCCGTGCGCCAGGCACGTCCGGGCCGACCGTTCGGGCATTGCGCGGCGCCGCGCCAGAGCGGGATTTCGGCCAGGACATCGCCGAGCTGCGTGATTACCTGGCCGACAACGGCAAACGCCGGGTGCGCGGCGTACCGGGTGCGCACGATGTGCCGGTGTGGATCCTCGGTTCCAGCCTGTTCGGTGCTGACCTGGCGGCGA  >  KT2440/3586277‑3586541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑