Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,626,1060TG40.0% 42.8 / 12.4 55V174G (GTG→GGG) hcf136_3Ycf48‑like protein
Reads supporting (aligned to +/- strand):  ref base T (17/16);  new base G (0/22);  total (17/38)
Fisher's exact test for biased strand distribution p-value = 1.85e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CAGGCAGCCGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGC  >  KT2440/3625970‑3626240
                                                                                                                                        |                                                                                                                                       
caggctgCCGCGGCTGCCGCAGACGCGCGGCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGt                                                                                                                                         <  2:372965/137‑1 (MQ=255)
   gcagCCGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGc                                                                                                                                      >  3:383805/1‑137 (MQ=255)
   gcagCCGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGc                                                                                                                                      >  4:45474/1‑137 (MQ=255)
   gcagCCGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGc                                                                                                                                      >  4:272892/1‑137 (MQ=255)
   gcagCCGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGc                                                                                                                                      >  5:448626/1‑137 (MQ=255)
   gcagCCGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGGTGTTGGCCCTGAATTTCACCCATGCCCGCAACGGCCTGGTCGTCGGTGCCGACGGGGTGGc                                                                                                                                      >  2:178452/1‑137 (MQ=255)
        cGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCACCGGCTGCTGGCCGCCGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATgc                                                                                                                                 >  6:492926/1‑137 (MQ=255)
        cGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCGACGGGGTGGCCATgc                                                                                                                                 >  7:439143/1‑137 (MQ=255)
                             tCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCG‑CCGACCGCGGGCGCa                                                                                                           >  3:299269/1‑137 (MQ=255)
                              cGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCG‑CCGACGGCGGGCGCAg                                                                                                          >  6:103620/1‑137 (MQ=255)
                              cGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCG‑ACGACGGCGGGCGCAg                                                                                                          >  5:186885/1‑137 (MQ=255)
                                   ggggggggcgAAACGGGTGGTGGCCGAGGGCGCGGAAAAGCGGTTGTTGGCCCTTAATTTCCCCTTGGCCCGAAACGGCCGGGGCGTGGGGGCCTCCGGGGGGGCCATGCGCACCG‑AGGACGGCGGGCGCAGCTGGc                                                                                                     <  4:129092/131‑1 (MQ=255)
                                     ggggggcgCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCACCGGCCTGGTCGGCGGTGCCTACGGGGGGGCCATGCGCACCG‑ACGACGGCGGGCGCAGCTGGCAg                                                                                                   <  5:410710/133‑1 (MQ=255)
                                             cAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTTCCTACGGGGTGGCCATGCGCACCG‑CCGACGGCGGGCGCAGCTGGCAGTCGGAGAt                                                                                           >  6:18211/1‑137 (MQ=255)
                                               aCGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCg                                                                                         <  4:383805/137‑1 (MQ=255)
                                               aCGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTCAATTTCACCGATGCCCGCACCGGCGTGGGCGGCGGTGCCTACGGGGGGGCCATGCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCg                                                                                         <  5:492926/137‑1 (MQ=255)
                                                cGGCTGCTGGCCCACGGCGCGGCCAAGCCGTTGTTGGCCCTGAAATACACCGAGGCCCCCACCGGCCGGGGCGGCGGGGCCTACGGGGGGGCCATGCCCACCG‑AGGACGGCGGGCGCAGCTGGCAGTCGGAGATCgg                                                                                        <  4:305776/137‑1 (MQ=255)
                                                 ggCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGc                                                                                       >  2:25517/1‑137 (MQ=255)
                                                       gggCGGAGGGCGCGGACAAGCCGTTGTTGGCCGTGAATTTCACCGAGGCCCGCAACGGCGTGGGGTTCGGTGCCTCCGGGGGGGCCATGCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTg                                                                                 <  5:101747/136‑1 (MQ=255)
                                                         gCCGTCTGCGCGGACAAGGCTTTGTTGGCCCTGAATTTCACCGATGCCCGCACCGGCCTGGTCGGCGGGGCCTACGGGGGGGCCAGGCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGcc                                                                               <  2:64419/137‑1 (MQ=255)
                                                         gCCGACGGCGCGGACAAGCCGTTGTTGGCCCTTAATTTCACCGATGCCCGAAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGcc                                                                               <  6:484494/137‑1 (MQ=255)
                                                         gCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGAGGCCCGCAACGGCCTGGGCGGCGGGGCCTACGGGGGGGCCATGCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGcc                                                                               <  5:215008/137‑1 (MQ=255)
                                                         ccctaCGGCGCGGACAAGCCGTTGTGGGCCCTTAATTGCACCGCTGCCCGCACCGGCCGGGGGGGGGGTGCCTAGGGGGTGGCCTTGCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGcc                                                                               <  4:129618/133‑1 (MQ=255)
                                                         cccgccgGCGGGCACAAGCGTTTTTCGGCCCTATATTCCCCCGCGCCCCCCACCGGCCGGGGCGGGGGGGCGACCGGGGGGCCCAGGCCCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGcc                                                                               <  8:184241/136‑1 (MQ=255)
                                                         cccGACGGCGGGGACAAGCCGTTGTTGGCCCTTAATTTCACCGATGCCCGCACCGGCCGGGGCGGCGGTGCCTCCGGGGGGCCCATGCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGcc                                                                               <  8:342729/136‑1 (MQ=255)
                                                              gggCGCGGAAAAGCCGTTGTTGGCCCTTAATTTCACCGATGCCCGCACCGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACg                                                                          <  7:280511/136‑1 (MQ=255)
                                                               ggCGCGGACAAGCGTTGGTGGGCCCTGAATTGCCCCGATCCCCGCACCGGCCGGGTCGGCGGGGCCTACGGGGTGGCCATGCCCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACgg                                                                         <  3:122954/137‑1 (MQ=255)
                                                               ggCGCGGACAAGCGGTTGTTGGCCCTTAATTTCACCGATCCCCGCACCGGCCTGGGCGGCGGGGCCTCGGGGGGGGCCATGCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACgg                                                                         <  3:89184/137‑1 (MQ=255)
                                                                      acacGCGGTTGTGGGCCCTGAATTTCACCGATGCCCGCATGGGCCTGGGGGGGGGGCCCTACGGGGTGCCCAGGCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGa                                                                  <  1:34786/137‑1 (MQ=255)
                                                                      aCATGCGGTTGTTGCCCATTAATTACACCGATCCCCCCAACGGCGGGGGCGGGGGGGCCTCGGGGGTGGCAATGCCCACCC‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGTCGAACGGGCGTGGa                                                                  <  3:272892/137‑1 (MQ=255)
                                                                        acgCCGTTGTTGGCCCTTAATTTCACCGAGGCCCCCAACGGCGTGGTGGGGGGTGCCTACGGGGGGGCACTGCGCACCT‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACt                                                                <  1:352557/135‑1 (MQ=255)
                                                                        acgCCGTTGTGGGCCCTTAATTCCCCCGAGCCCCCCCCCGCCCGGGGCGGCGGGGCCTCGGGGGGGCCCAGGCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACt                                                                <  3:126942/135‑1 (MQ=255)
                                                                        aaGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGGCGTCGGGGCCTACGGGGTGGCCATGCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACt                                                                <  4:440316/137‑1 (MQ=255)
                                                                         cgCCGTTGTGGGCCCTGAATTTCCCCGAGGCCCGCAACGGCCGGGGCGGCGGTGCCTCCGGGGGGGCCATGCGCCCCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTg                                                               <  4:149005/136‑1 (MQ=255)
                                                                             gttcttGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGGCGTGGCCTACGGGGTGGCCATGCGCCCCT‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCAcc                                                           <  6:130365/137‑1 (MQ=255)
                                                                              ttgttgGCCCTGAATTTCCCCGAGGCCCGCACCGGCCTGGTCGTCGGTGCCTACGGGGGGGCCATGCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCt                                                          <  4:351048/137‑1 (MQ=255)
                                                                                 ttgGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGa                                                                  >  5:361791/1‑126 (MQ=255)
                                                                                 ttgGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGGCGGTGCCTACGGGGGGGCCATGCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGa                                                                  <  6:361791/126‑1 (MQ=255)
                                                                                  gggCCCTGAATTTCACCGATGCCCGCACCGGCCTGGTCGGCGGGGCCTCCGGGGGGGCCATGCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTAt                                                      <  1:77365/136‑1 (MQ=255)
                                                                                            tttCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGgcgc                                            >  8:414899/1‑137 (MQ=255)
                                                                                             ttCACCGATCCCCGCACCGGCCTGGGCGTCGGGGCCTCCGGGGGGGCCAGGCGCCCCG‑CCGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGgcgcg                                           <  6:266551/137‑1 (MQ=255)
                                                                                               acccGATGCCGCCACCGGCGTGTCGGTCGGGGCCTTCGGGGTGGCCTTG‑GCCCCGTACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGcc                                         <  8:71078/135‑1 (MQ=255)
                                                                                                    cgccccGCACCGGCGGGGGCGTGGGTGCCTCCGGGGGGCCAAGGCCCACCG‑CCGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGc                                    <  3:14468/134‑1 (MQ=255)
                                                                                                    aggCCCGCACCGGCCTGGTCGTCGGTGCCTACGGGGGGGCCATGCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGc                                    <  7:100308/135‑1 (MQ=255)
                                                                                                    aTGCCCGCACCGGCCTGGGCGTCGGTGCCTACGGGGTGGCCATGCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGc                                    <  2:288391/137‑1 (MQ=255)
                                                                                                       cccGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAAc                                 >  5:266551/1‑137 (MQ=255)
                                                                                                       cccGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAAc                                 >  1:234667/1‑137 (MQ=255)
                                                                                                       cccGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCCAACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAAc                                 <  2:329759/137‑1 (MQ=255)
                                                                                                       cccGCAACGGCCTGGGCGTCGGGGCCTACGGGGGGGCCATGCGCCCCG‑AGGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAAc                                 <  2:100837/137‑1 (MQ=255)
                                                                                                            aagggCCTGGGCGGCGGTGCCTAGGGGGTGGCCATGCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGgt                            <  1:72476/134‑1 (MQ=255)
                                                                                                                     ggcgtcgGTGCCTCCGGGGTGCCCATGCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGc                   <  5:478457/135‑1 (MQ=255)
                                                                                                                     ggcggcgGGGCCTCCGGGGGGCCCCTGCCCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGc                   <  2:191867/132‑1 (MQ=255)
                                                                                                                           ggggCCTACGGGGTGCCGATGCGCACCT‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGa             <  8:261100/134‑1 (MQ=255)
                                                                                                                             ggCCTCCGGGGGGGCCAGGCCCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGc           <  3:222545/136‑1 (MQ=255)
                                                                                                                             ggCCTAGGGGGTGGCCAGGCGCCCCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGc           <  6:186885/136‑1 (MQ=255)
                                                                                                                                      ggTGGCCATGCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCctgc  >  5:490300/1‑137 (MQ=255)
                                                                                                                                        |                                                                                                                                       
CAGGCAGCCGAGGCTGCCGCAGACGAGCGTCGCCTGGCGGCGGCGCAACGGCTGCTGGCCGACGGCGCGGACAAGCCGTTGTTGGCCCTGAATTTCACCGATGCCCGCAACGGCCTGGTCGTCGGTGCCTACGGGGTGGCCATGCGCACCG‑ACGACGGCGGGCGCAGCTGGCAGTCGGAGATCGGCCGACTGCCGAACGGGCGTGGACTGCACCTGTATGCCCTGGCGCGCCAAGGCAACCAGGTGTACATCGCCGGCGAGCAGGGCCTGC  >  KT2440/3625970‑3626240

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑