| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 4,025,810 | 0 | C | A | 53.8% | 5.5 / 17.0 | 13 | intergenic (+58/‑198) | dmdC_5/CJ019_03801 | 3‑methylmercaptopropionyl‑CoA dehydrogenase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (2/4); new base A (0/7); total (2/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.92e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGGTTGGGGCTGGAGGCGAGCATTCGGGCCGGTAGTGGCAGCCTTTATGGGCTAGAGGCCACACAGTTCTGACCGGTGGGTTGCC‑GACGCGCTTGTTGCAAGGGCAGCCGTGTGCTGCGAAGAGGCCTGCAAACACTGACCTCTTCGCAGCACCAGACTGCCCCGGAAGCGATTGCAGGCATCGTTTTTTGCCCGCGAAGGGTTCTCAAACACTGAAAAACCGTGGCTAATCCGACGAAAAACCGGCACAACTGATAGTCATTC > KT2440/4025681‑4025944 | gggTTGGGGCTGGAGGCGAGCATTCGGGCCGGTAGTGGCAGCCTTTATGGGCTAGAGGCCACACAGTTCTGACCGGTGGGGTGCC‑GACGCGCTTGTTGCAAGGGCAGCCGTGTGCTGCGAAGAGGCCTGCAAACACt < 2:50341/137‑1 (MQ=255) gTTGGGGCTGGAGGCGAGCATGCGGGCCGGTAGTGGCAGCCTTTATGGGCTAGAGGCCACACAGTTCTGACCGGTCGGTTGCC‑GACGCGCTTGTTGCAAGGGCAGCCGTGTGCTGCGAAGAGGCCTGCAAACACTGa < 7:445285/137‑1 (MQ=255) ggCTGGAGGCGAGCATTCGGGCCGGTAGTGGCAGCCTTTATGGGCTAGAGGCCACACAGTTCTGACCGGTGGGTTGCC‑GACGCGCTTGTTGCAAGGGCAGCCGTGTGCTGCGAAGAGGCCTGCAAACACTGACctct < 3:19698/137‑1 (MQ=255) ggCTGGAGGCGAGCATTCGGGCCGGTAGTGGCAGCCTTTATGGGCTAGAGGCCACACAGTTCTGACCGGTGGGTTGCC‑GACGCGCTTGTTGCAAGGGCAGCCGTGTGCTGCGAAGAGGCCTGCAAACACTGACctct < 7:458188/137‑1 (MQ=255) ggCTGGAGGCGAGCATTCGGGCCGGTAGTGGCAGCCTTTAGTGGCTAGAGGCCACACAGTTCTGACCGGTGGGTTGCC‑GACGCGCTTGTTGCAAGGGCAGCCGTGTGCTGCGAAGAGGCCTGAAAACACTGACctct < 6:460676/137‑1 (MQ=255) ggCCAAACTGTTCTCCCCGGGGGGTTGCC‑GACGCGCTTGTTGCACGGGCTGCGGTGGGCTGCGAAGAGCCCTCAAAACACTGACCTCTTGGCAGCACCAGACTGCCCCGGAAGCGATTGCAGGCATCGTTTTTTGcc < 6:164916/137‑1 (MQ=255) cccacaGTTCTGCCCGGGGGGTGGCC‑GACGCGCTTGGTGCTCGGGCAGCGGTGTGCGGCGAAGAGCCCTGAAAACAATGACCTCTGCGCAGCACCAGACTGCCCCGGAAGCGATTGCAGGCATCGTTTTTTGCCcgc < 6:246607/135‑1 (MQ=255) gtgccGGGGGGGTGCCGGCCGCGTTTTTTGCTGGGCCAGCGGTGTGGTGCTATGAGGCCTGAAAACACTCCCCTGTTCGAAGCACCAGACTGCCCCGGAAGCGATTGCAGGCATCGTTTTTTGCCCGCGAAGGGTtc < 4:446541/134‑1 (MQ=255) caCCGGTGGGTTCCC‑GACGCGCTTGTTCCAAGGGCAGCCGTGTGCGGCTAAGAGGCCTCAAAACACTCACCTCTGCGCAGCACCAGACTGCCCCGGAAGCGATTGCAGGCATCGTTTTTTGCCCGCGAAGGGTtctc < 5:384089/136‑1 (MQ=255) gctaaGCGGCTTGAAAACACTGACCTCTTCCCAGCACCAGACTGCCCCGGAAGCGATTGCAGGCATCGTTTTTTGCCCGCGAAGGGTTCTCAAACACTGAAAAACCGTGGCTAATCCGACGAAAAACCGGCACAACt < 3:308447/134‑1 (MQ=255) ggCCTCAAAACACTTACCTCTTCGCAGCACCAGACTGCCCCGGAAGCGATTGCAGGCATCGTTTTTTGCCCGCGAAGGGTTCTCAAACACTGAAAAACCGTGGCTAATCCGACGAAAAACCGGCACAACTGATAGTc < 1:55643/137‑1 (MQ=255) tGCAAACACTGACCTCTTCGCAGCACCAGACTGCCCCGGAAGCGATTGCAGGCATCGTTTTTTGCCCGCGAAGGGTTCTCAAACACTGAAAAACCGTGGCTAATCCGACGAAAAACCGGCACAACTGATAGTCATTc > 8:304286/1‑137 (MQ=255) tGCAAACACTGACCTCTTCGCAGCACCAGACTGCCCCGGAAGCGATTGCAGGCATCGATTTTTGCCCGCGAAGGGTTCTCAAACACTGAAAAACCGTGGCTAATCCGACGAAAAACCGCCACAACTGATAGTCATTc > 5:469942/1‑137 (MQ=255) | GGGTTGGGGCTGGAGGCGAGCATTCGGGCCGGTAGTGGCAGCCTTTATGGGCTAGAGGCCACACAGTTCTGACCGGTGGGTTGCC‑GACGCGCTTGTTGCAAGGGCAGCCGTGTGCTGCGAAGAGGCCTGCAAACACTGACCTCTTCGCAGCACCAGACTGCCCCGGAAGCGATTGCAGGCATCGTTTTTTGCCCGCGAAGGGTTCTCAAACACTGAAAAACCGTGGCTAATCCGACGAAAAACCGGCACAACTGATAGTCATTC > KT2440/4025681‑4025944 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |