Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,384,2760CG36.0% 74.7 / 18.9 51intergenic (+539/‑319)CJ019_05064/CJ019_05067putative protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (3/29);  new base G (17/1);  total (21/30)
Fisher's exact test for biased strand distribution p-value = 1.90e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

CGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGT  >  KT2440/5384168‑5384403
                                                                                                            |                                                                                                                               
cGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGaata                                                                                                     <  2:294616/137‑3 (MQ=255)
    tAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTGTGTGGCATTTTGGGGCGGCCGGGCGCAAAAtaa                                                                                                 >  8:19084/1‑135 (MQ=255)
     aaGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTGTGGCGGGTATTTGGGCGCTGCGGGTGGAAAAATCgg                                                                                                >  6:143330/1‑137 (MQ=255)
        cATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAATGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGAtt                                                                                             <  5:200149/137‑1 (MQ=255)
        cATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGAtt                                                                                             <  7:124712/137‑1 (MQ=255)
        cATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGAtt                                                                                             <  8:350440/137‑1 (MQ=255)
           ttagtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTaa                                                                                          <  1:362198/136‑1 (MQ=255)
           gtagtaCCGACAGCGGAGTACTGGCGGACGTGGGTGCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGCGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTaa                                                                                          <  2:120606/137‑1 (MQ=255)
           gtagtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCTGGCGTCTTTTTTTGCGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTaa                                                                                          <  2:127200/137‑1 (MQ=255)
           gtagtaCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTaa                                                                                          <  1:155824/137‑1 (MQ=255)
             agtaCCGAGAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAAcc                                                                                        <  4:312794/137‑1 (MQ=255)
                aCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGGTTTTTTTTGCTGGATTTTTGGGGCGGGGGGGGGAAAAATAGGATTTaaaacca                                                                                     >  1:310339/1‑132 (MQ=255)
                aCCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCGGTTTTTTTGGCTGGGATTTTGGGGGTGCGGGGCGGAAAATCGGTTTTAAcacca                                                                                     >  2:362198/1‑136 (MQ=255)
                    aCAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTTGCTCGAATTTGGTGGCTGGGGGGCGAAAAATCCGATTTAAACCCattat                                                                                 >  5:484520/1‑135 (MQ=255)
                    aCAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCGTTTTTTGTGGCGGGTATTTGGGGGCGGGGGGTCGAAAAATGGGATTTAACCCCGTTcg                                                                                 >  8:414744/1‑136 (MQ=255)
                    aCAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTAGGGCGTCGTTTTTTGTGCCAGGGATTTGTGCGCTGCGGGTAGAAAAATCCGATTTACCCCCtttgt                                                                                 >  6:466179/1‑135 (MQ=255)
                     cAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGTTTTTTTTTGGCTGGAATTTTTGGGCTGCGGGTGGAAAAAAAGGATTTAACCCCATTCtt                                                                                >  6:424053/1‑137 (MQ=255)
                     cAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTGTTTTTTTGCTGGGATTTTGGCGATGGGGGGGGAAAAAAAGGTTTTAAACCCattatt                                                                                >  7:91817/1‑137 (MQ=255)
                       gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGTGTTTTTGTGGCAGGAATATGGGCGCGGGGGGGCGCAAAAACGGTGTTTAACCCTTTCTTGt                                                                              >  2:40540/1‑137 (MQ=255)
                       gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTGTGGCTGGAATTTTGGGGGTGCGGGTGGAAAAATCGGATTTAAACCCattatttt                                                                              >  6:198220/1‑135 (MQ=255)
                       gCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTTTTGCGGGAATTTGGGCGCTGCGGGGCGAAAAAAAGGATTTAACCCCGTTCTTGt                                                                              >  8:108992/1‑137 (MQ=255)
                          gAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTTTTCGTGGAATTTGGGGGGGGCGGGTCGAAAAATAGGATTTTACACCGTTTGTGTAgg                                                                           >  2:69845/1‑137 (MQ=255)
                           tgTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAggg                                                                          <  3:284513/136‑1 (MQ=255)
                           aGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAggg                                                                          <  2:389787/137‑1 (MQ=255)
                                 ggCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                                    <  1:495074/137‑1 (MQ=255)
                                 ggCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTc                                                                    <  3:90039/137‑1 (MQ=255)
                                     gACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTGGCTGGTATTTTGGGGGGGGGGGGGGGAAAATCGGGTTTTACACCGGTATTGTAGGGCGgttggcca                                                                >  4:461013/1‑132 (MQ=255)
                                          gggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTGGCTGGTATTTTGGCGGTGCGGGGGGAAAAATAGGATTTTA‑ACCATTGTTGTAGGGCGGTTGCCAAAGATaa                                                          >  6:423972/1‑136 (MQ=255)
                                          gggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTTTGGCGGGTTTTTTGGGGCGGGGGGGGGTAAAATAGGCTTTTAACCCGTTGTTGTTTGGCGGTTGCCCAAGAtt                                                           >  4:460683/1‑136 (MQ=255)
                                          gggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGTTTTTTGTGGCTGGGATTTGGGGGGGGCGGGGGGAAAAATCGGCTTTAACCCCGTTCTTGTTGGGGGGTTGCCAAAGATa                                                           >  7:90337/1‑137 (MQ=255)
                                          gggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTGGGTTTTTGTGGCGGGGATTTGGGGGGGGCGGGGGGGAAAAAAGGATTTAACCCCGTTCTAGTTGGGCGGTTGGCAAAgctt                                                           >  7:272857/1‑134 (MQ=255)
                                          gggTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCGTTTTTTTTTCGTGGATTTTTGGGGCTGCGGGGGGAAAAAAGGGATTTAGACCCTTTGTTTTATGGGGGTTGGCAAAgtat                                                           >  4:288113/1‑134 (MQ=255)
                                          gggTTCAAATAACCCCGGATCCACCAAATCCAACGAAGAAGACGACCTAGTGCGTCTTTTTTTGTTCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATa                                                           <  6:265261/137‑1 (MQ=255)
                                            gTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATAct                                                         <  8:498619/137‑1 (MQ=255)
                                            gTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATAct                                                         <  5:369993/137‑1 (MQ=255)
                                                      ccccGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCt                                               <  7:281788/137‑1 (MQ=255)
                                                                accaAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGtt                                     <  3:82527/137‑1 (MQ=255)
                                                                            gaagaagaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt                         <  5:499594/137‑1 (MQ=255)
                                                                            gaagaagaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt                         <  5:143330/137‑1 (MQ=255)
                                                                            gaagaagaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt                         <  8:318659/137‑1 (MQ=255)
                                                                            gaagaagaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGGTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCAttttt                         <  7:448510/137‑1 (MQ=255)
                                                                              agaagaCGCCCTCGGGCGTGGTTTTTTTTTGGTGGGATTTTTGGGGGGGGGGGGGAAAAATAGGATTTTAACCCGTTGGTGTTGGGGGGTTGCCCAAAATAATGCCTGCGCCGGTTTTTCGTTGGGGTGTttttttt                       >  6:315647/1‑136 (MQ=255)
                                                                              agaagaCGCCCTCGGGCGTCTTTTTTTGTGGCTGGGATTTGGGGGCTGCGGGGGGTAAAATCGGATTTATCACCTTTCTTGTTGGGCGTTTAGCAGACATACTGCCATCGCCGGTTTTGCGTTTCGGGCAttttttt                       >  8:392571/1‑136 (MQ=255)
                                                                                 agaCGCCCTCTGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAgg                    <  6:423759/137‑1 (MQ=255)
                                                                                 agaCGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAgg                    <  4:459358/137‑1 (MQ=255)
                                                                                      cccTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGtttt               <  7:108992/137‑1 (MQ=255)
                                                                                           gggCGTCTTTTTTTGTGGCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACAACTTTCTTGTAGGGCGTTTCGCAATGATACTGCCTGCGCGTGTTTTCCGTTGCGGTCATTTTTTCAGGTTTTTAGtct          <  2:428666/137‑1 (MQ=255)
                                                                                           gggCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGGTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtct          <  1:80098/137‑1 (MQ=255)
                                                                                            ggCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGtctc         <  5:424053/137‑1 (MQ=255)
                                                                                                tCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGGCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGGCTGTTTTCCGTTGCGGTCATTTTTTAAGTGTTTTAGTCTCGGTg     <  5:396236/137‑1 (MQ=255)
                                                                                                   ttttttGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGt  <  7:239961/137‑1 (MQ=255)
                                                                                                            |                                                                                                                               
CGGCTAAGCATGTAGTACCGACAGCGGAGTACTGGCGGACGGGGGTTCAAATCCCCCCGGCTCCACCAAATCCAAAGAAGAAGACGCCCTCGGGCGTCTTTTTTTGTGCCTGGAATTTGGGCGCTGCGGGTCGAAAAATCGGATTTAACCCCTTTCTTGTAGGGCGTTTCCCAAAGATACTGCCTGCGCCTGTTTTCCGTTGCGGTCATTTTTTCAGGGTTTTAGTCTCGGTGGGT  >  KT2440/5384168‑5384403

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑