Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	5,689,816	0	A	C	39.1%	34.0 / 14.5	66	S236R (AGC→CGC)	pyrC'	Dihydroorotase‑like protein
Reads supporting (aligned to +/- strand):  ref base A (10/29);  new base C (7/18);  total (17/49)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCG  >  KT2440/5689682‑5689950                                                                                                                                       |                                                                                                                                       tGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCGCTTCAGCCAGATCACCCgc                                                                                                                                      >  6:4102/1‑137 (MQ=255) tGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACATCAGCCAGCTAACCacc                                                                                                                                      >  7:455821/1‑135 (MQ=255)  ggCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATAACCCgcc                                                                                                                                     >  2:24747/1‑136 (MQ=255)       aCGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCCCATGAGCCAGATCACCAGCcccccg                                                                                                                                >  1:25067/1‑135 (MQ=255)           gggTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGCAGAGCGCCTAAAACGTGGCCCTGGCGCGAAACCTGCGGCTGGTGTAACATAGCGGCGTGCGTGCGAACTTCCGCCAGATCACCAGCGCCCGTGgcg                                                                                                                            <  6:508227/136‑1 (MQ=255)           aGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCCGCCCCCCTGgcg                                                                                                                            >  3:315960/1‑137 (MQ=255)                ccATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCgg                                                                                                                       >  5:84345/1‑137 (MQ=255)                ccATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTAAGCCCTATAACCCGCGCCCCTGgcgcgccg                                                                                                                       >  8:89814/1‑135 (MQ=255)                 cttGGCCAGCTTCCTGGGCCTGCCGGGCATCCCGGCCCGCCCCCAACCGGGGGCCCGGGCGCGCAACCTGCTGGGGGGGCAACCGCGCGGCGGGCGGGCGCACTTCACCCAGACCACCACCGCCCGGGGCGCGCGGc                                                                                                                      <  2:453009/135‑1 (MQ=255)                 cATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGATCAGAGCGGCGTGGGGGGGCACTTCAGGCAGAGCACCCGCCCCCCGGGCGCGCggg                                                                                                                      >  3:76297/1‑136 (MQ=255)                      ccAGCTTCCTCGGCCTGCGGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATc                                                                                                                 <  8:337131/137‑1 (MQ=255)                      ccAGCTTCCTCGGCCTGCCGGGCATCCCGGATAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGGGGCGTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATc                                                                                                                 <  3:188467/137‑1 (MQ=255)                      ccAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATc                                                                                                                 <  3:373385/137‑1 (MQ=255)                      ccAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGCCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCACTTCCGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATc                                                                                                                 <  1:68836/137‑1 (MQ=255)                      ccAGCTTCCTCGGCCTGCCGGGCAGCCCGGAGAGCGCCTAAACCGTGGCCCGGGCGCGCAACCTGCGGCTGGGGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATc                                                                                                                 <  7:452973/137‑1 (MQ=255)                      ccAGCTCCCTCGGCCTGCCGGGCAGCCCGGAGAGCGCCGAAACCGTGGCCCGGGCGCGCACCCTGCGGCGGGTGGAACAGAGCGGCGTGCGTGCGCACTTCCGCCAGAGCACCCGCGCCCGTGGCGCGCGGCTGATc                                                                                                                 <  5:128661/137‑1 (MQ=255)                       cAGCTTCCTCGGCCTGCCGGGCACCCCGGAGAGCGCCTAAACCGGGGCCCTGGCGCGCACCCTGCTGCTGGGGCAACAGAGCGGCGTGCGGGCGCACTGCAGCCAGAGCCCCAGCGCCCGTGGCGCGCGGCTGATCg                                                                                                                <  7:300980/137‑1 (MQ=255)                            gccTCTGCCTGCCGGGCATCCCGGCGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGATGGTGGAACAGAGCGGCGGGCGTGCGCACTTCAGCCAGATCATCAGCGCCCGTGGCGCGCGGCTGATCgcccag                                                                                                           <  7:445441/136‑1 (MQ=255)                             ccTGGGCCTGCGGGGCAGCCCGTTGAGCGCCGAAACGGGGGCCGTGGCGCGCAACGGGCTGCTGGTGGAACAGAGCGGCGGGGGTGCGCAGTTCAGCCTGATCACCAGCGCCGGTGGCGCGCGGCTGATCgcccagg                                                                                                          <  4:54182/137‑1 (MQ=255)                             ccTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGGGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCgcccagg                                                                                                          <  8:86016/137‑1 (MQ=255)                             ccTCGGCCGGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGAAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCAGTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCgcccagg                                                                                                          <  2:115530/137‑1 (MQ=255)                              ggcGGCCTGCGGGGCATCCGGGAGCGGGCCTAACCGGTCGCCCGGCCGCGCAACCTGCGGGGGGGGTAACAGTGGGGGGGGGGTGCGCACTGCAGCCATATCCCCAGCGCCCGGGGCGCGCGGCTGATCgcccaggc                                                                                                         <  8:180295/135‑1 (MQ=255)                              cTCGGCCTGCCGGGCATCCGGTTGTGCGCCTAAACCGTGGCCCTGGCGGGAAACCTGGTGGTGGTGTAACTGTGCGGGGGGGGTGCGCACTTCCGCCAGATCACACGCGCCGGGGGGGCGCGGCTGATCgcccaggc                                                                                                         <  4:118453/137‑1 (MQ=255)                                        ggggCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTg                                                                                               <  6:423183/136‑1 (MQ=255)                                           gCATCCCGGAGAGCGCCGAACCCGGGGCCCTGGCGCGCAACCTGCTGCGGGGGCACCAGCGCGGCGGGCGTGCGCACTCCCCCCAGAACACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGc                                                                                            <  3:365403/137‑1 (MQ=255)                                                   gcgagCGCCGAAACCGGGGCCCTGGCGCGCACCCTGCTGCTGGTGGAACCGCGCGGCGGGCGGGCGCACTTCAGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGt                                                                                    <  3:320104/135‑1 (MQ=255)                                                   gagaGCGCCGAAACCGGGGCCCTGGCGCGCAACCTGCTGCTGGGGGAACAGAGCGGCGTGCGGGCGCACTTCACCCAGAACACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGt                                                                                    <  5:441234/137‑1 (MQ=255)                                                      cgcgcCGAAACCGGGGCCCGGGCGCGCAACCTGCTGCTGGTGCAACAGCGCGGCGTGCGGGCGCACTTCCGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                                 <  2:316344/136‑1 (MQ=255)                                                          ccGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGGGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGGAAccgcc                                                                             >  1:80037/1‑137 (MQ=255)                                                           ctaacccGGGGCCCTGGCGCGCAACCTGGGGCTGGTGCAACCGAGCGGCGGGCGTGCGCACTACAGCCCTAACCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                                            <  8:18149/132‑1 (MQ=255)                                                           cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                                            <  7:363179/137‑1 (MQ=255)                                                                ggggggCCCGGGCGCGAAACCGGCTGGTGGTGCAACAGCGCGGGGTGCGGGCGCACTTCAGCCAGAACACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                       <  4:449218/133‑1 (MQ=255)                                                                gcggggCCCGGGCGCTAAACCTGCTGCTGGTGCAACAGTGCGGCGTGCGGGCGCATTTCACCAAGATCCCCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                       <  1:118877/133‑1 (MQ=255)                                                                ccGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                       <  5:245543/137‑1 (MQ=255)                                                                ccGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                       <  5:354628/137‑1 (MQ=255)                                                                ccGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                       <  6:114988/137‑1 (MQ=255)                                                                ccGTGGCCCTGGCGCGCAACCTGCTGCTGGGGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                       <  2:26853/137‑1 (MQ=255)                                                                ccGTGGCCCTGGCGCGCAACCTGCGGCGGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                       <  2:313961/137‑1 (MQ=255)                                                                ccGTGGCCCGGGCGCGAAACCTGGTGCTGGTGGAACAGAGCGGCGTGGGTGCGCACTTCCGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                       <  3:94558/137‑1 (MQ=255)                                                                ccGGGGCCCTGGGGCGCAACCTGCGGGTGGTGTAACAGAGCGGGGTGCGGGCGAACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                       <  3:152337/137‑1 (MQ=255)                                                                ccGGGGCCCGGGCGCGCAACCTGCTGCTGGTGGACCAGAGCGGCGTGCGGGCGCACTTCCGCCATATCACCAGGGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                       <  6:326043/137‑1 (MQ=255)                                                                     ccccTGGCGCGCAACCTGCGGCTGGGGCAACAGCGCGGCGGGCGGGCGCACTCCCGCCACACCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCt                                                                  <  3:347255/136‑1 (MQ=255)                                                                      cccTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTAAGCCACATCACCACCGCCCCTGGCGCGCGGCTGCTAGCCCACGCCCCGGCCGTGTGGCTGCCGGTAACCGCCGCTGTAgcgcgg                                                                 >  8:76994/1‑135 (MQ=255)                                                                            ggcgcAACCTGCTGCTGGTGGAACAGAGCGGGGTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAg                                                           <  7:116632/136‑1 (MQ=255)                                                                                        ggcgggtggATCAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCTCGGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCATGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACg                                               <  8:120151/133‑1 (MQ=255)                                                                                           tggtggAACAGAGCGGCGTGCGTGCGCACTTAAGCCAGATCACCCGCGCCCGTGGCGCGCCGCTGATCGCCCCGGCCCCCGGCGTGGGCCTGCCGGGAACCGCCGATGTTGCGCGGTACCAGCGGATACTccccgcc                                            >  2:125665/1‑131 (MQ=255)                                                                                              tggAACAGAGCGGCGTGCGGGCGCACTTCAGCCAGATAACCAGCGCCCGTGGCGCGCGGCTGATCGCCCACGCCCCGGCCGTGGGCCTGCCGGGTACCGCCGCTGTAGCGCTGTACCACCTGATACTCAAGGACGAg                                         >  8:269671/1‑137 (MQ=255)                                                                                                gAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATAACCAGCGCCCGTGGCGCGCGGCTGATCGCCCACGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGCTGTAGCGCTGTACCAGCTGATCCTCACGGCCGAGTc                                       >  8:165093/1‑137 (MQ=255)                                                                                                      cgcggcgTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg                                 <  5:348386/136‑1 (MQ=255)                                                                                                       gcgccgTGCGTGCGAAGTCCCGCCAGAGCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcgc                                <  2:479222/137‑1 (MQ=255)                                                                                                           ggtgcgtgcGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                            <  1:329390/136‑1 (MQ=255)                                                                                                           ggtgcgtgcGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                            <  5:104410/136‑1 (MQ=255)                                                                                                           ggggcgtgcgCACTTCAGCCAGATCACCCGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                            <  5:338368/134‑1 (MQ=255)                                                                                                           ggggcgtgcgCACTTCACCCATAACACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                            <  1:125665/134‑1 (MQ=255)                                                                                                           cgtgcgtgCGCACTTCAGCCAGATCCCCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                            <  7:145683/137‑1 (MQ=255)                                                                                                           cgggcgtgcgCACTTCACCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                            <  5:434546/134‑1 (MQ=255)                                                                                                             ggcgtgcgCACTTCAGCCAGATCCCCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTtc                          <  2:157055/136‑1 (MQ=255)                                                                                                             ggcgtgcgCACTGCAGCCACATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTtc                          <  4:128188/136‑1 (MQ=255)                                                                                                                gtgcgCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTcc                       <  2:287661/137‑1 (MQ=255)                                                                                                                   cgcACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGGTCTCCAGc                    >  8:94450/1‑137 (MQ=255)                                                                                                                     caagtgaGCCCGATCACCTCGGCTGGTGGCGCGAGGCTTATCTCCGAGGCCCTGGCATTGGTCCTGCCTGTAACCTCCTATGTATCGCTGTACCAGCTGATCCTCTCGGACGAGTCGCTGCGCGAGTTCTCCAGCCt                  <  7:105388/131‑1 (MQ=255)                                                                                                                     cACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCt                  <  6:350692/137‑1 (MQ=255)                                                                                                                       ataccgCCTGATCACCCCGGCCGGGGGCGCGCGGATGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGt                <  2:217500/132‑1 (MQ=255)                                                                                                                            gCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCAc           >  2:149420/1‑137 (MQ=255)                                                                                                                                   cACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAgc    >  4:127314/1‑137 (MQ=255)                                                                                                                                     ccAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCCGCCTGTACCACTTGCAgccg  >  2:428524/1‑137 (MQ=255)                                                                                                                                     ccAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCCCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAgccg  >  8:64991/1‑137 (MQ=255)                                                                                                                                       |                                                                                                                                       TGGCCCACGAAGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCG  >  KT2440/5689682‑5689950

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑