Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	KT2440	5,689,824	0	T	G	31.4%	73.0 / 11.5	70	R238R (CGT→CGG)	pyrC'	Dihydroorotase‑like protein
Reads supporting (aligned to +/- strand):  ref base T (16/32);  new base G (1/21);  total (17/53)
Fisher's exact test for biased strand distribution p-value = 1.41e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCGCCGCTGCGC  >  KT2440/5689692‑5689959                                                                                                                                     |                                                                                                                                        gggTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGCAGAGCGCCTAAAACGTGGCCCTGGCGCGAAACCTGCGGCTGGTGTAACATAGCGGCGTGCGTGCGAACTTCCGCCAGATCACCAGCGCCCGTGgcg                                                                                                                                     <  6:508227/136‑1 (MQ=255) aGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCCGCCCCCCTGgcg                                                                                                                                     >  3:315960/1‑137 (MQ=255)      ccATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCgg                                                                                                                                >  5:84345/1‑137 (MQ=255)      ccATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTAAGCCCTATAACCCGCGCCCCTGgcgcgccg                                                                                                                                >  8:89814/1‑135 (MQ=255)       cttGGCCAGCTTCCTGGGCCTGCCGGGCATCCCGGCCCGCCCCCAACCGGGGGCCCGGGCGCGCAACCTGCTGGGGGGGCAACCGCGCGGCGGGCGGGCGCACTTCACCCAGACCACCACCGCCCGGGGCGCGCGGc                                                                                                                               <  2:453009/135‑1 (MQ=255)       cATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGATCAGAGCGGCGTGGGGGGGCACTTCAGGCAGAGCACCCGCCCCCCGGGCGCGCggg                                                                                                                               >  3:76297/1‑136 (MQ=255)            ccAGCTTCCTCGGCCTGCGGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATc                                                                                                                          <  8:337131/137‑1 (MQ=255)            ccAGCTTCCTCGGCCTGCCGGGCATCCCGGATAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGGGGCGTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATc                                                                                                                          <  3:188467/137‑1 (MQ=255)            ccAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATc                                                                                                                          <  3:373385/137‑1 (MQ=255)            ccAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGCCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGGGCGCACTTCCGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATc                                                                                                                          <  1:68836/137‑1 (MQ=255)            ccAGCTTCCTCGGCCTGCCGGGCAGCCCGGAGAGCGCCTAAACCGTGGCCCGGGCGCGCAACCTGCGGCTGGGGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATc                                                                                                                          <  7:452973/137‑1 (MQ=255)            ccAGCTCCCTCGGCCTGCCGGGCAGCCCGGAGAGCGCCGAAACCGTGGCCCGGGCGCGCACCCTGCGGCGGGTGGAACAGAGCGGCGTGCGTGCGCACTTCCGCCAGAGCACCCGCGCCCGTGGCGCGCGGCTGATc                                                                                                                          <  5:128661/137‑1 (MQ=255)             cAGCTTCCTCGGCCTGCCGGGCACCCCGGAGAGCGCCTAAACCGGGGCCCTGGCGCGCACCCTGCTGCTGGGGCAACAGAGCGGCGTGCGGGCGCACTGCAGCCAGAGCCCCAGCGCCCGTGGCGCGCGGCTGATCg                                                                                                                         <  7:300980/137‑1 (MQ=255)                  gccTCTGCCTGCCGGGCATCCCGGCGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGATGGTGGAACAGAGCGGCGGGCGTGCGCACTTCAGCCAGATCATCAGCGCCCGTGGCGCGCGGCTGATCgcccag                                                                                                                    <  7:445441/136‑1 (MQ=255)                   ccTGGGCCTGCGGGGCAGCCCGTTGAGCGCCGAAACGGGGGCCGTGGCGCGCAACGGGCTGCTGGTGGAACAGAGCGGCGGGGGTGCGCAGTTCAGCCTGATCACCAGCGCCGGTGGCGCGCGGCTGATCgcccagg                                                                                                                   <  4:54182/137‑1 (MQ=255)                   ccTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGGGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCgcccagg                                                                                                                   <  8:86016/137‑1 (MQ=255)                   ccTCGGCCGGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGAAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCAGTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCgcccagg                                                                                                                   <  2:115530/137‑1 (MQ=255)                    ggcGGCCTGCGGGGCATCCGGGAGCGGGCCTAACCGGTCGCCCGGCCGCGCAACCTGCGGGGGGGGTAACAGTGGGGGGGGGGTGCGCACTGCAGCCATATCCCCAGCGCCCGGGGCGCGCGGCTGATCgcccaggc                                                                                                                  <  8:180295/135‑1 (MQ=255)                    cTCGGCCTGCCGGGCATCCGGTTGTGCGCCTAAACCGTGGCCCTGGCGGGAAACCTGGTGGTGGTGTAACTGTGCGGGGGGGGTGCGCACTTCCGCCAGATCACACGCGCCGGGGGGGCGCGGCTGATCgcccaggc                                                                                                                  <  4:118453/137‑1 (MQ=255)                              ggggCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTg                                                                                                        <  6:423183/136‑1 (MQ=255)                                 gCATCCCGGAGAGCGCCGAACCCGGGGCCCTGGCGCGCAACCTGCTGCGGGGGCACCAGCGCGGCGGGCGTGCGCACTCCCCCCAGAACACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGc                                                                                                     <  3:365403/137‑1 (MQ=255)                                         gcgagCGCCGAAACCGGGGCCCTGGCGCGCACCCTGCTGCTGGTGGAACCGCGCGGCGGGCGGGCGCACTTCAGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGt                                                                                             <  3:320104/135‑1 (MQ=255)                                         gagaGCGCCGAAACCGGGGCCCTGGCGCGCAACCTGCTGCTGGGGGAACAGAGCGGCGTGCGGGCGCACTTCACCCAGAACACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGt                                                                                             <  5:441234/137‑1 (MQ=255)                                            cgcgcCGAAACCGGGGCCCGGGCGCGCAACCTGCTGCTGGTGCAACAGCGCGGCGTGCGGGCGCACTTCCGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAc                                                                                          <  2:316344/136‑1 (MQ=255)                                                ccGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGGGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGGAAccgcc                                                                                      >  1:80037/1‑137 (MQ=255)                                                 ctaacccGGGGCCCTGGCGCGCAACCTGGGGCTGGTGCAACCGAGCGGCGGGCGTGCGCACTACAGCCCTAACCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                                                     <  8:18149/132‑1 (MQ=255)                                                 cGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAAccgccg                                                                                     <  7:363179/137‑1 (MQ=255)                                                      ggggggCCCGGGCGCGAAACCGGCTGGTGGTGCAACAGCGCGGGGTGCGGGCGCACTTCAGCCAGAACACCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                                <  4:449218/133‑1 (MQ=255)                                                      gcggggCCCGGGCGCTAAACCTGCTGCTGGTGCAACAGTGCGGCGTGCGGGCGCATTTCACCAAGATCCCCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                                <  1:118877/133‑1 (MQ=255)                                                      ccGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                                <  5:354628/137‑1 (MQ=255)                                                      ccGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                                <  6:114988/137‑1 (MQ=255)                                                      ccGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                                <  5:245543/137‑1 (MQ=255)                                                      ccGTGGCCCTGGCGCGCAACCTGCTGCTGGGGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                                <  2:26853/137‑1 (MQ=255)                                                      ccGTGGCCCTGGCGCGCAACCTGCGGCGGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                                <  2:313961/137‑1 (MQ=255)                                                      ccGTGGCCCGGGCGCGAAACCTGGTGCTGGTGGAACAGAGCGGCGTGGGTGCGCACTTCCGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                                <  3:94558/137‑1 (MQ=255)                                                      ccGGGGCCCTGGGGCGCAACCTGCGGGTGGTGTAACAGAGCGGGGTGCGGGCGAACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                                <  3:152337/137‑1 (MQ=255)                                                      ccGGGGCCCGGGCGCGCAACCTGCTGCTGGTGGACCAGAGCGGCGTGCGGGCGCACTTCCGCCATATCACCAGGGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTa                                                                                <  6:326043/137‑1 (MQ=255)                                                           ccccTGGCGCGCAACCTGCGGCTGGGGCAACAGCGCGGCGGGCGGGCGCACTCCCGCCACACCCCCCGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCt                                                                           <  3:347255/136‑1 (MQ=255)                                                            cccTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTAAGCCACATCACCACCGCCCCTGGCGCGCGGCTGCTAGCCCACGCCCCGGCCGTGTGGCTGCCGGTAACCGCCGCTGTAgcgcgg                                                                          >  8:76994/1‑135 (MQ=255)                                                                  ggcgcAACCTGCTGCTGGTGGAACAGAGCGGGGTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAg                                                                    <  7:116632/136‑1 (MQ=255)                                                                              ggcgggtggATCAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCTCGGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCATGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACg                                                        <  8:120151/133‑1 (MQ=255)                                                                                 tggtggAACAGAGCGGCGTGCGTGCGCACTTAAGCCAGATCACCCGCGCCCGTGGCGCGCCGCTGATCGCCCCGGCCCCCGGCGTGGGCCTGCCGGGAACCGCCGATGTTGCGCGGTACCAGCGGATACTccccgcc                                                     >  2:125665/1‑131 (MQ=255)                                                                                    tggAACAGAGCGGCGTGCGGGCGCACTTCAGCCAGATAACCAGCGCCCGTGGCGCGCGGCTGATCGCCCACGCCCCGGCCGTGGGCCTGCCGGGTACCGCCGCTGTAGCGCTGTACCACCTGATACTCAAGGACGAg                                                  >  8:269671/1‑137 (MQ=255)                                                                                      gAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATAACCAGCGCCCGTGGCGCGCGGCTGATCGCCCACGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGCTGTAGCGCTGTACCAGCTGATCCTCACGGCCGAGTc                                                >  8:165093/1‑137 (MQ=255)                                                                                            cgcggcgTGCGTGCGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcg                                          <  5:348386/136‑1 (MQ=255)                                                                                             gcgccgTGCGTGCGAAGTCCCGCCAGAGCCCCCGCGCCGGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTgcgc                                         <  2:479222/137‑1 (MQ=255)                                                                                                 ggtgcgtgcGCACTTCAGCCAGATCACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                                     <  1:329390/136‑1 (MQ=255)                                                                                                 ggtgcgtgcGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                                     <  5:104410/136‑1 (MQ=255)                                                                                                 ggggcgtgcgCACTTCAGCCAGATCACCCGCGCCGGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                                     <  5:338368/134‑1 (MQ=255)                                                                                                 ggggcgtgcgCACTTCACCCATAACACCCGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                                     <  1:125665/134‑1 (MQ=255)                                                                                                 cgtgcgtgCGCACTTCAGCCAGATCCCCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                                     <  7:145683/137‑1 (MQ=255)                                                                                                 cgggcgtgcgCACTTCACCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGt                                     <  5:434546/134‑1 (MQ=255)                                                                                                   ggcgtgcgCACTTCAGCCAGATCCCCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTtc                                   <  2:157055/136‑1 (MQ=255)                                                                                                   ggcgtgcgCACTGCAGCCACATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTtc                                   <  4:128188/136‑1 (MQ=255)                                                                                                      gtgcgCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTcc                                <  2:287661/137‑1 (MQ=255)                                                                                                         cgcACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGGTCTCCAGc                             >  8:94450/1‑137 (MQ=255)                                                                                                           caagtgaGCCCGATCACCTCGGCTGGTGGCGCGAGGCTTATCTCCGAGGCCCTGGCATTGGTCCTGCCTGTAACCTCCTATGTATCGCTGTACCAGCTGATCCTCTCGGACGAGTCGCTGCGCGAGTTCTCCAGCCt                           <  7:105388/131‑1 (MQ=255)                                                                                                           cACTTCAGCCAGATCACCAGCGCCCGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCt                           <  6:350692/137‑1 (MQ=255)                                                                                                             ataccgCCTGATCACCCCGGCCGGGGGCGCGCGGATGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGt                         <  2:217500/132‑1 (MQ=255)                                                                                                                  gCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCAc                    >  2:149420/1‑137 (MQ=255)                                                                                                                         cACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAgc             >  4:127314/1‑137 (MQ=255)                                                                                                                           ccAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCCGCCTGTACCACTTGCAgccg           >  2:428524/1‑137 (MQ=255)                                                                                                                           ccAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCCCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAgccg           >  8:64991/1‑137 (MQ=255)                                                                                                                                gcCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCGCCGCt      <  4:315960/137‑1 (MQ=255)                                                                                                                                  ccGTGGCGCGCGGCTGATATCACAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCGCCAGCCTGTACCACGTGCAGCCGCCGCTgc    >  7:226924/1‑137 (MQ=255)                                                                                                                                  ccGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCGCCGCTgc    <  5:4102/137‑1 (MQ=255)                                                                                                                                  ccGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCGCCGCTgc    <  1:24747/137‑1 (MQ=255)                                                                                                                                  ccGGGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCCCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCGCCGCTgc    <  7:64991/137‑1 (MQ=255)                                                                                                                                    gTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCCGCCTGTACCACGTGCAGCCGCCGCTgcgc  >  1:376604/1‑137 (MQ=255)                                                                                                                                    gTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCGCCGCTgcgc  >  5:363928/1‑137 (MQ=255)                                                                                                                                     |                                                                                                                                        AGGTGCCATGGCCAGCTTCCTCGGCCTGCCGGGCATCCCGGAGAGCGCCGAAACCGTGGCCCTGGCGCGCAACCTGCTGCTGGTGGAACAGAGCGGCGTGCGTGCGCACTTCAGCCAGATCACCAGCGCCCGTGGCGCGCGGCTGATCGCCCAGGCCCAGGCCTTGGGCCTGCCGGTAACCGCCGATGTAGCGCTGTACCAGCTGATCCTCACGGACGAGTCGCTGCGCGAGTTCTCCAGCCTGTACCACGTGCAGCCGCCGCTGCGC  >  KT2440/5689692‑5689959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑