Predicted mutation
evidence seq id position mutation annotation gene description
RA KT2440 3,957,060 A→T Y197N (TAC→AAC)  dmlR_13 ← HTH‑type transcriptional regulator DmlR

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24403,957,0600AT98.4% 212.8 / ‑4.7 61Y197N (TAC→AAC) dmlR_13HTH‑type transcriptional regulator DmlR
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base T (30/30);  total (31/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.47e-01

CGGCAGCAGGCCAATGCCCATGCCCAGCTCGCTGGCCACCAGCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGC  >  KT2440/3956929‑3957186
                                                                                                                                   |                                                                                                                              
cGGCAGCAGGCCAATGCCCATGCCCAGCTCGCTGGCCACCAGCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCg                                                                                                                           <  1:52398/137‑1 (MQ=255)
      cagGCCAATGCCCATGCCCAGCTCGCTGGCCACCAGCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAg                                                                                                                     >  5:145977/1‑137 (MQ=255)
      cagGCCAATGCCCATGCCCAGCTCGCTGGCCACCAGCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAg                                                                                                                     >  7:4303/1‑137 (MQ=255)
          ccAATGCCCATGCCCAGCTCGCTGGCCACCAGCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCAt                                                                                                                 <  3:283217/137‑1 (MQ=255)
               gcccatgcccaGCTCGCTGGCCACCAGCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCATGCgca                                                                                                            <  2:373556/137‑1 (MQ=255)
                      cccaGCTCGCTGGCCACCAGCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCg                                                                                                     >  6:87031/1‑137 (MQ=255)
                      cccaGCTCGCTGGCCACCAGCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCAAGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTGGTTCATCGGGTAAGCCATGCGCAGGCAGCg                                                                                                     >  8:135765/1‑137 (MQ=255)
                       ccaGCTCGCTGGCCACCAGCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGa                                                                                                    <  5:442221/137‑1 (MQ=255)
                             cGCTGGCCACCAGCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCGGGCGTTGATCGGGGAAGCCATGGGCCGGGAGCGCTGGGCg                                                                                              >  2:146352/1‑137 (MQ=255)
                             cGCTGGCCACCAGCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCg                                                                                              <  5:203331/137‑1 (MQ=255)
                              gCTGGCCACCAGCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCGGTCGTTCATCGGGTAAGCCATGACCACGCAGCGATGGcccg                                                                                             >  6:321938/1‑135 (MQ=255)
                              gCTGGCCACCAGCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGGTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCgg                                                                                             >  7:481737/1‑136 (MQ=255)
                              gCTGGCCACCAGCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCGTCGTTCCTCGGGTTAGCCATGCGCAGGCAGCGATGGCCgg                                                                                             >  1:24310/1‑136 (MQ=255)
                              gCTGGCCACAAGCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGt                                                                                             <  4:328576/137‑1 (MQ=255)
                               cTGGCCACCAGCATGGCGTCGGCGACGTTTGTAGAGAAACTGTCGTTTGGCTCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTACGCCATGCGCAGGCAGCGATGGCCGTg                                                                                            <  7:317397/137‑1 (MQ=255)
                               cTGGCCACCAGCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTg                                                                                            <  5:12824/137‑1 (MQ=255)
                                   ccaccaGCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCCTGCGCAGGCAGCGATGGCCGTGCCAg                                                                                        >  6:393655/1‑137 (MQ=255)
                                        aGCATGGCGTCGGCGACGTTGGGAGAGAAACTGTCGTTTGGCGCGATCAAGCACTGGTCAACCCCTTTCGGGAACACCCATTCGCCTTGGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGTCCGTGCAAGTCTTc                                                                                   <  4:69955/137‑1 (MQ=255)
                                         gCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCg                                                                                  <  7:308589/137‑1 (MQ=255)
                                         gCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCg                                                                                  <  1:224183/137‑1 (MQ=255)
                                           aTGGCGTCGGCGACGTTTGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCggg                                                                                <  6:142161/137‑1 (MQ=255)
                                           aTGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCggg                                                                                <  3:359987/137‑1 (MQ=255)
                                           aTGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCggg                                                                                <  1:413383/137‑1 (MQ=255)
                                           aTGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCggg                                                                                <  5:358208/137‑1 (MQ=255)
                                           aTGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCggg                                                                                <  5:87031/137‑1 (MQ=255)
                                           aTGGCGTCGGCGACGGTGGTAGAGAAACTGGCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCggg                                                                                <  6:410865/137‑1 (MQ=255)
                                             ggCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGgc                                                                              <  7:44738/137‑1 (MQ=255)
                                              gCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCAAGCGCAGGCACCCATGGCCGTGGAAGTCGTCGGGgcc                                                                             >  3:184602/1‑136 (MQ=255)
                                               cGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGgcgc                                                                            <  5:12784/137‑1 (MQ=255)
                                               cGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGgcgc                                                                            <  1:464141/137‑1 (MQ=255)
                                                 tCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTc                                                                          <  1:264115/137‑1 (MQ=255)
                                                 tCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCGTCGTTGGGCGGGTTAGCCATGCGCAGGCAGCGAAGGACGGTGAAGTCGTAGGGGCGCtg                                                                          >  3:285263/1‑136 (MQ=255)
                                                 gcGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTc                                                                          <  7:471614/136‑1 (MQ=255)
                                                   ggCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCg                                                                        <  4:468219/137‑1 (MQ=255)
                                                    gCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCgg                                                                       >  7:273545/1‑137 (MQ=255)
                                                           tGGTAGAGAAACTGTCGTTTGGCTCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCg                                                                <  2:489787/137‑1 (MQ=255)
                                                           tGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCg                                                                <  6:431666/137‑1 (MQ=255)
                                                           tGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCg                                                                <  8:121890/137‑1 (MQ=255)
                                                            ggTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCGTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGt                                                               >  3:396000/1‑137 (MQ=255)
                                                                          cGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTGATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACCCCGTGGGTGTCCAGGTAg                                                 >  5:357527/1‑137 (MQ=255)
                                                                                gcgcGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCCGGCCCCGATGGCCGTGCAAGTCGTCGGGGCGCTCCGGTACACCGGGGGGGTCCAGGTAGCCgggg                                           >  8:106546/1‑137 (MQ=255)
                                                                                     aTCATGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTAATAGGGTAAGCCATTCGCAGGCAGCGATGGCCGTGCAAGTCTTAGGGGCGCTCCCGTACACCCGGGGTTTGCAGGTAGCCGGGGGCcgc                                      >  5:63418/1‑137 (MQ=255)
                                                                                         cGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAg                                  <  5:433605/137‑1 (MQ=255)
                                                                                                   aaCCCCTTGCGGGAACACCCAGTCGCCTTCGTTCATCGCGTAAGCCATTCCCACGCAGCGATGGCCGTGCAAGTATTCCGGGCGCTCCGGTACACCGTCGGAGTCCAGGTAGCCCGGGGCCGCGCAGGGGATGCTGa                        >  7:451054/1‑137 (MQ=255)
                                                                                                   aaCCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGa                        >  8:468098/1‑137 (MQ=255)
                                                                                                   aaCCCCTTGCGGGAACACCCAGTCGCCATCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGa                        >  7:214679/1‑137 (MQ=255)
                                                                                                      cccTTGCGGGAACACCCAGTCGCCGTCGTTCAACGGGTAACCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTACGGTACACCGGGGGTGTCCAGGTAGGCGGGGGCCGCGCAGGGGATGGTGaaaa                     >  8:245593/1‑137 (MQ=255)
                                                                                                        cTTGCGGAAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTATTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAata                   >  1:110525/1‑137 (MQ=255)
                                                                                                          tGCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATAtt                 <  3:480236/137‑1 (MQ=255)
                                                                                                           gCGGGAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGTAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAataatt                >  8:449092/1‑134 (MQ=255)
                                                                                                             gggAACACCCAGTCGCGTTCGTTGATCGGGTTAGCCATGCGCAGGCAGCGATGGCCGTGGAAGTCTTCGGGGCGCTCCCGTAAACCGTGGGTGTGCAGGTAGCCGGGGGCCGCGCAGGGGGTGCTGAAAATAtttac              >  3:92665/1‑134 (MQ=255)
                                                                                                             gggAACACCCAGTCGCGTTCGTTCATCGGGTAAGCCAAGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGcc              >  7:16653/1‑137 (MQ=255)
                                                                                                             gggAACACCCAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGcc              >  5:421845/1‑137 (MQ=255)
                                                                                                             gggAACACCCAGTCGCCTTCGTTCATCGGGTAACCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTGCAGGTATCCGGGGGCCGCGCAGGGGATGCTGAAAATATTTcc              >  8:378176/1‑137 (MQ=255)
                                                                                                             gggAACACCCAGTCGCCGTCGGTCATAGGGTAAGCCATGCGCAGGCAGCGATTGCCGGGCAAATCTTCGGGGCGCGCCCGTACCCCGGGGGGGGCCAGGTTGCCGGGGGCCGCGCCGGGGATGCGGAAAataattgc              >  2:226418/1‑132 (MQ=255)
                                                                                                             gggAACACACAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCCTGGGATGCTGAAAATATTTcc              >  6:211948/1‑137 (MQ=255)
                                                                                                                  cacCCAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCg         >  2:262371/1‑137 (MQ=255)
                                                                                                                    cccAGTCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGc       <  5:510488/137‑1 (MQ=255)
                                                                                                                    cccAGTCGCCTTCGTTCATCGGGTAAGCCAAGCGCAGGCAGCGATGGCCGTGCAAGTCTTAGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCCCAGGGGATGCGGAAAATATTTCCCAGCGGc       >  8:368690/1‑137 (MQ=255)
                                                                                                                      cAGTCGCGTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGcac     >  8:204384/1‑137 (MQ=255)
                                                                                                                         tCGCCTTCGTTCATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGc  <  8:27983/137‑1 (MQ=255)
                                                                                                                                   |                                                                                                                              
CGGCAGCAGGCCAATGCCCATGCCCAGCTCGCTGGCCACCAGCATGGCGTCGGCGACGTTGGTAGAGAAACTGTCGTTTGGCGCGATCACGCACTGGTCAACCCCTTGCGGGAACACCCAGTCGCCTTCGTACATCGGGTAAGCCATGCGCAGGCAGCGATGGCCGTGCAAGTCTTCGGGGCGCTCCGGTACACCGTGGGTGTCCAGGTAGCCGGGGGCCGCGCAGGGGATGCTGAAAATATTGCCCAGCGGCACAGC  >  KT2440/3956929‑3957186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑