| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 5,026,996 | C→T | 12.1% | intergenic (+335/‑75) | yabJ_3 → / → CJ019_03742 | 2‑iminobutanoate/2‑iminopropanoate deaminase/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 5,026,996 | 0 | C | T | 12.1% | 91.1 / 4.5 | 34 | intergenic (+335/‑75) | yabJ_3/CJ019_03742 | 2‑iminobutanoate/2‑iminopropanoate deaminase/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (13/16); new base T (2/2); total (16/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.52e-01 | |||||||||||
ACAGCTGGTCGCCGACCGGCTCACTCAGTGGGGCTTGGAGATTCACCGTGGACTGGCAAAAACCGGGGTCGTTGGAACGCTCGTTGGACGCTCGCCAGGAATCACCATCGTAAGCGTCCGGCGAACTCACCTTCCGAACTCCAGCATTAAGGGCGATGGTGTCCGCGTATTTTTAAGCGGACGCGATCACCCTTATCGCCAGGATTTCTATGCGCCAACGAAGCTCTTATCCGAAACCGT > KT2440/5026862‑5027101 | acaGCTGGTCGCCGACCGGCTCACTCAGTGGGGCTTGGAGATTCACCGTGGACTGGCAAAAACCGGGGTCGTTGGAACGCTCGTTGGCCGCTCGCCAGGCATCACCATCGTAAGCGTCCGGCGAACACCCCATcc > 2:359049/1‑135 (MQ=255) aGCTGGTCGCCGACCGGCTCACTCAGTGGGGCTTGGAGATTCACCGTGGACTGGCAAAAACCGGGGTCGTTGGAACGCTCGTTGGACGCTCGCCAGGAATCACCATCGTAAGCGTCCGGCGAACTCACCTTCCGa > 3:197916/1‑135 (MQ=255) gggggTCGCCGACGGGCTCACTCAGTGGGGCTTGGTGATTCACCGTGGACTGGCAAAAACCGGGGTCGTTGGAACGCGCGTTGGACGCTCGCCAGGAATGACCATCGTAAGGGGGCGGCGAACTCACCTTCCGaa < 8:381060/132‑1 (MQ=255) gCTGGTCGCCGACCGGCTCACTCAGTGGGGCTTGGAGATTCACCGTGGACTGGCAAAAACCGGGGTCGTTGGAACGCTCGTTGGACGCTCGCCAGGAATCACCATCGTAAGCGTCCGGCGAACTCACCTTCCGaa > 2:100811/1‑135 (MQ=255) cTGGTCGCCGACCGGCTCACTCAGTGGGGCTTTGAGATTCACCGTGGACTGGCAAAAACCGGGGTCGTTGGAACGCTCGTTGGACGCTCGCCAGGAATCACCATCGTAAGCGTCCGGCGAACTCACCTTCCGAAc < 7:55842/135‑1 (MQ=255) cTGGTCGCCGACCGGCTCACTCAGTGGGGCTTGGAGATTCACCGTGGACTGGCAAAAACCGGGGTCGTTGGAACGCTCGTTGGACGCTCGCCAGGAATCACCATCGTAAGCGTCCGGCGAACTCACCTTCCGAAc < 7:211161/135‑1 (MQ=255) gCCGACCGGCTCACTCAGTGGGGCTTGGAGATTCACCGTGGACTGGCAAAAACCGGGGTCGTTGGAACGCTCGTTGGCCGCTCGCCAGGAATCACCATCGTAAGCGTCCGGCGAACTCACCTTACGAACTCCAg > 5:64688/1‑134 (MQ=255) gCCGACCGGCTCACTCAGTGGGGCTTGGAGATTCACCGTGGACTGGCAAAAACCGGGGTCGTTGGAACGCTCGTTGGACGCTCGCCAGGAATCACCATCGTAAGCGTCCGGCGAACTCACCTTCCGAACTCCAg > 8:193618/1‑134 (MQ=255) gCCGACCGGCTCACTCAGTGGGGCTTGGAGATTCACCGTGGACTGGCAAAAACCGGGGTCGTTGGAACGCTCGTTGGACGCTCGCCAGGAATCACCATCGTAAGCGTCCGGCGAACTCACCTTCCGAACTCCAGc < 4:31722/135‑1 (MQ=255) gCTCACTCAGTGGGGCTTGGAGATTCACCGTGGACTGGCAAAAACCGGGGTCGTTGGAACGCTCGTTGGACGCTCGCCAGGAATCACCATCGTAAGCGTCCGGCGAACTCACCTTCCGAACTCCAGCATTAAggg < 5:80001/135‑1 (MQ=255) gCTCACTCAGTGGGGCTTGGAGATTCACCGTGGACTGGCAAAAACCGGGGTCGTTGGAACGCTCGTTGGACGCTCGCCAGGAATCACCATCGTAAGCGTCCGGCGAACTCACCTTCCGAACTCCAGCATTAAggg < 7:193618/135‑1 (MQ=255) ggggCTTGGAGATTCACCGTGGACTGGCAAAAACCGGGGTCGTTGGAACGCTCGTTGGACGCTCGCCAGGAATCACCATCGTAAGCGTCCGGCGAACTCACCTTCCGAACTCCAGCATTAAGGGCGATGGTGTcc < 1:319776/135‑1 (MQ=255) ggCTTGGAGATTCACCGTGGACTGGCAAAAACCGGGGTCGTTGGAACGCTCGTTGGACGCTCGCCAGGAATCACCATCGTAAGCGTCCGGCGAACTCACCTTCTGAACTCCAGCATTAAGGGCGATGGTGTCcgc > 8:252771/1‑135 (MQ=255) ggCTTGGAGATTCACCGTGGACTGGCAAAAACCGGGGTCGTTGGAACGCTCGTTGGACGCTCGCCAGGAATCACCATCGTAAGCGTCCGGCGAACTCACCTTCCGAACTCCAGCATTAAGGGCGATGGTGTCcgc > 6:362241/1‑135 (MQ=255) ggCTTGGAGATTCACCGTGGACTGGCAAAAACCGGGGTCGTTGGAACGCTCGTTGGACGCTCGCCAGGAAACACCATCGTAAGCGTCCGGCGAACTCACCTTCTGAACTCCAGCATTAAGGGCGATGGTGTCcgc > 8:258995/1‑135 (MQ=255) ggAGATTCACCGTGGACTGGCAAAAACCGGGGTCGTTGGAACGCTCGTTGGACGCTCGCCAGGAATCACCATCGTAAGCGTCCGGCGAACTCACCTTCCGAACTCCAGCATTAAGGGCGATGGTGTCCGCGTAtt > 3:329436/1‑135 (MQ=255) gaTTCACCGTGGACTGGCAAAAACCGGGGTCGTTGGAACGCTCGTTTGACGCTCGCCAGGAATCACCATAGTAAGCGTCCGGCGAACTCACCTTCCGAACTCCAGCATTAAGGGCGATGGTGTACGCGTAtttt > 6:167458/1‑134 (MQ=255) gaTTCACCGTGGACTGGCAAAAACCGGGGTCGTTGGAACGCTCGTTGGACGCTCGCCAGGAATCACCATCGTAAGCGTCCGGCGAACTCACCTTCCGAACTCCAGCATTAAGGGCGATGGTGTCCGCGTAtttt > 3:112041/1‑134 (MQ=255) aCCGTGGACTGGCAAAAACCGGGGTCGTTGGAACGCTCGTTGGACGCTCGCCAGGAATCACCATCGTAAGCGTCCGGCGAACTCACCTTCCGAACTCCAGCATTAAGGGCGATGGTGTCCGCGTATTTTTAAGc < 4:197916/134‑1 (MQ=255) ccGTGGACTGGCAAAAACCGGGGTCGTTGGAACGCTCGTTGGACGCTCGCCAGGAATCACCATCGTAAGCGTCCGGCGAACTCACCTTCCGAACTCCAGCATTAAGGGCGATGGTGTCCGCGTATTTTTAAGCgg > 3:297069/1‑135 (MQ=255) ggACTGGCAAAAACCGGGGTCGTTGGAACGCTCGTTGGACGCTCGCCAGGAATCACCATCGTAAGCGTCCGGCGAACTCACCTTCCGAACTCCAGCATTAAGGGCGATGGTGTCCGCGTATTTTTAAGCGGAcgc < 8:176463/135‑1 (MQ=255) cAAAAACCGGGGGCGTTGGAACGCTCGGTGGACGCTCGCCAGGAATCACCATCGTAAGCGTCCGGCGAACTCACCTTCCGAACTCCAGCATTAAGGGCGATGGTGTCCGCGTATTTTTAAGCGGACGCGATCAcc < 6:305485/135‑1 (MQ=255) aaaaCCGGGGTCGTTGGAACGCTCGTTGGAAGCTCGCCAGGAATCAACATCGTAAGCGTCCGGCGAACTCACCTTCAGAACTCCAGCATTAAGGGCGATGGTGTCCGCGTATTTTTAAGCGGACGCTCTCAccc > 6:361428/1‑134 (MQ=21) tCGTTGGAACGCTCGTTGGACGCTCGCCAGGAATCACCATCGTAAGCGTCCGGCGAACTCACCTTCCGAACTCCAGCATTAAGGGCGATGGTGTCCGCGTATTTTTAAGCGGACGCGCTCACCCTTATCGCCa > 1:192803/1‑133 (MQ=255) gtgggACGCGCGCCAGGAATCACCATCGTAAGCGTCCGGCGAACTCACCTTCCGAACTCCAGCATTAAGGGCGATGGTGTCCGCGTATTTTTAAGCGGACGCGATCACCCTTATCGCCAGGATTTCTATGCGCCa < 1:359049/132‑1 (MQ=255) cGCTCGCCAGGAATCACCATCGTAAGCGTCCGGCGAACTCACCTTCCGAACTCCAGCATTAAGGGCGATGGTGTCCGCGTATTTTTAAGCGGACGCGATCACCCTTATCGCCAGGATTTCTATGCGCCAACGAAg < 6:64688/135‑1 (MQ=255) cGCTCGCCAGGAATCACCATCGTAAGCGTCCGGCGAACTCACCTTCCGAACTCCAGCATTAAGGGCGATGGTGTCCGCGTATTTTTAAGCGGACGCGATCACCCTTATCGCCAGGATTTCTATGCGCCAACGAAg < 5:362241/135‑1 (MQ=255) cGCTCGCCAGGAATCACCATCGTAAGCGTCCGGCGAACTCACCTTCCGAACTCCAGCATTAAGGGCGATGGTGTCCGCGTATTTTTAAGCGGACGCGATCACCCTTATCGCCAGGATTTCTATGCGCCAACGAAg < 3:96102/135‑1 (MQ=255) cAGGAATCACCATCGTAAGCGTCCGGCGAACTCACCTTCCGAACTCCAGCATTAAGGGCGATGGTGTCCGCGTATTTTTAAGCGGACGCGATCACCCTTATCGCCAGGATTTCTATGCGCCAACGAAGCTCTTAt > 2:317588/1‑135 (MQ=40) ccATCGTAAGCGTCCGGCGAACTCACCTTCCGAACTCCAGCATTAAGGGCGATGGTGTCCGCGTATTTTTAAGCGGACGCGATCACCCTTATCGCCAGGATTTCTATGCGCCAACGAAGCTCTTATCCGAAACCg > 3:198468/1‑135 (MQ=33) cATCGTAAGCGTCCGGCGAACTCACCTTCTGAACTCCAGCATTAAGGGCGATGGTGTCCGCGTATTTTTAAGCGGACGCGATCACCCTTATCGCCAGGATTTCTATGCGCCAACGAAGCTCTTATCCGAAACCGt < 7:252771/135‑1 (MQ=17) cATCGTAAGCGTCCGGCGAACTCACCTTCTGAACTCCAGCATTAAGGGCGATGGTGTCCGCGTATTTTTAAGCGGACGCGATCACCCTTATCGCCAGGATTTCTATGCGCCAACGAAGCTCTTATCCGAAACCGt < 7:258995/135‑1 (MQ=17) cATCGTAAGCGTCCGGCGAACTCACCTTCCGAACTCCAGCATTAAGGGCGATGGTGTCCGCGTATTTTTAAGCGGACGCGATCACCCTTATCGCCAGGATTTCTATGCGCCAACGAAGCTCTTATCCGAAACCGt < 1:73702/135‑1 (MQ=32) cATCGTAAGCGTCCGGCGAACTCACCTTCCGAACTCCAGCATTAAGGGCGATGGTGTCCGCGTATTTTTAAGCGGACGCGATCACCCTTATCGCCAGGATTTCTATGCGCCAACGAAGCTCTTATCCGAAACCGt < 1:100811/135‑1 (MQ=32) aTCGTAAGCGTCCGGCGAACTCACCTTCCGAACTCCAGCATTAAGGGCGATGGTGTCCGCGTATTTTTAAGCGGACGCGATCACCCTTATCGCCAGGATTTCTATGCGCCAACGAAGCTCTTATCCGAAACCGt > 1:47144/1‑134 (MQ=31) | ACAGCTGGTCGCCGACCGGCTCACTCAGTGGGGCTTGGAGATTCACCGTGGACTGGCAAAAACCGGGGTCGTTGGAACGCTCGTTGGACGCTCGCCAGGAATCACCATCGTAAGCGTCCGGCGAACTCACCTTCCGAACTCCAGCATTAAGGGCGATGGTGTCCGCGTATTTTTAAGCGGACGCGATCACCCTTATCGCCAGGATTTCTATGCGCCAACGAAGCTCTTATCCGAAACCGT > KT2440/5026862‑5027101 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |