| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | KT2440 | 5,073,255 | G→T | 7.7% | intergenic (‑674/+518) | CJ019_04767 ← / ← csrA2 | Magnesium transporter MgtE/Translational regulator CsrA2 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | KT2440 | 5,073,255 | 0 | G | T | 7.7% | 152.5 / 2.6 | 57 | intergenic (‑674/+518) | CJ019_04767/csrA2 | Magnesium transporter MgtE/Translational regulator CsrA2 |
| Reads supporting (aligned to +/- strand): ref base G (25/27); new base T (2/3); total (27/30) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01 | |||||||||||
GAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACC > KT2440/5073123‑5073382 | gAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCa < 5:156368/135‑1 (MQ=255) tGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAg < 4:188907/135‑1 (MQ=255) cGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGCGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGaa > 6:185981/1‑134 (MQ=255) tcagGTTTCCAACCTGCGTCCGCTCTGTTCGTAGCCGATTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCa < 7:144447/133‑1 (MQ=17) gcagGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCa < 3:385023/134‑1 (MQ=255) gATTCTAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCATAGCTATGAGTCCGCGATTGGCAGTTTTACACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGaaa < 1:117769/135‑1 (MQ=255) gAACCTGCGACCGCTCGGTTCGTAGCCGAGGACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGc < 3:23681/135‑1 (MQ=255) aCCTGCGAAGGCTCGGTTCGTAGCCGAGTAATCTATACAGCTGAGCTATGAGGCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCgg < 2:338550/135‑1 (MQ=17) gcTGCGAGCGGCCGGGTCGTAGCCGAGTACTCTATGCAGCTGTGCTATGAGTCCGGGTTTGGCAGTTTTAAACCAGTTTACCTCTGGTTTACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGa < 6:5789/134‑1 (MQ=14) cggacgctATCCAGCTGAGCTATGCGTCCGCGAATGGCAGTTTTACACCAGATTACCGCTGGATTACTTAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGAcc < 8:214060/129‑1 (MQ=11) aGTACTCTACCCCGCGGACCTATGCGGCCGCTATTGGCAGTTTTAAACCAGCTTACCCCTGGCTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGAcc < 4:144453/135‑1 (MQ=14) aCTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAg > 1:302030/1‑84 (MQ=255) aCTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAg < 2:302030/84‑1 (MQ=255) aCTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCt > 6:324329/1‑135 (MQ=255) ccAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTCCCGCGGGTTTACTGAACTGGCTTTGCAAGCAGCCCAACTTCAAGACAGTCGCGGACTCAGCAGGAATCGACCCTGCGACCGCTCGGTTCg > 5:4319/1‑135 (MQ=17) ccAGCTGAGCTATGAGTCCGCGAGTGGCAGTTTTAAACCAGATTACCGATGTTTGTATGAAATGGCTTTTCAAGAATAACCACTTCAAGAAAGTTGCGTCCTCAGCAGGAGACGAACCGCCGACCGCGCGGTTCg > 6:314423/1‑135 (MQ=17) ccAGCTGAGCTATGACTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTc < 5:324329/134‑1 (MQ=255) aGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTa > 5:207196/1‑135 (MQ=255) aGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTa > 1:201343/1‑135 (MQ=255) aGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTa > 1:289306/1‑135 (MQ=255) aGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTa < 1:58126/135‑1 (MQ=255) aGCTGAGCTATGAGTCCGCGATTGGCAGGTTTAAACCAGAATACCGCTGGTTGACTGAAAAGGCTTTGCAAGAAGAACCACTTCAATAAAGTGGCGGACTCATCAGGATACGAACCTGAGACCGGTCGGTTCGTa > 2:249345/1‑135 (MQ=17) aGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGAGTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGa > 1:209094/1‑135 (MQ=255) aGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAg > 8:150378/1‑70 (MQ=255) aGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAg < 7:150378/70‑1 (MQ=255) aGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGc > 8:295476/1‑132 (MQ=255) aGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGc < 7:295476/132‑1 (MQ=255) aGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGa > 7:140/1‑135 (MQ=255) gCTATGAGGGCGCGATTGTCAGTTTTAAACGAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGcc < 7:306964/132‑1 (MQ=255) cTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGt < 6:167452/135‑1 (MQ=255) tATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAg < 6:243030/67‑1 (MQ=255) tATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAg > 5:243030/1‑67 (MQ=255) tATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGACCCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTa > 1:382376/1‑135 (MQ=255) tATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGt < 5:185981/134‑1 (MQ=255) tGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGc > 3:299882/1‑127 (MQ=255) tGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGc < 4:299882/127‑1 (MQ=255) gAGGCCGCGCTTGGAAGGTTTAAACCAGTTTACCTCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTAct < 3:283863/134‑1 (MQ=14) gTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTCCTCTa > 3:219625/1‑135 (MQ=255) cgcgATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGACCCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATcc < 2:289306/135‑1 (MQ=255) cgcgATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATcc < 6:165490/135‑1 (MQ=255) cgcgATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATcc < 6:207196/135‑1 (MQ=255) gATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGc > 4:175524/1‑135 (MQ=255) gATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGc > 5:86655/1‑135 (MQ=255) aTTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGc > 1:289408/1‑134 (MQ=255) aTTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGc > 4:262045/1‑134 (MQ=255) cAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGGGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGc < 6:86655/134‑1 (MQ=255) gcttACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAAt < 6:254831/133‑1 (MQ=255) gTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGt < 6:132074/81‑1 (MQ=39) gTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGt > 5:132074/1‑81 (MQ=39) taCTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGa > 8:55315/2‑134 (MQ=11) gACTGAAATGGCTTTTCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAAt < 5:335992/121‑1 (MQ=11) cTGAAATGGCTTTTCAAGCTGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTGGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGAcc < 1:264923/134‑1 (MQ=12) aaaTGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTc > 7:260538/1‑75 (MQ=34) aaaTGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTc < 8:260538/75‑1 (MQ=34) tGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATAcc > 3:222405/1‑135 (MQ=40) tGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACAAGATAcc > 4:239028/1‑135 (MQ=255) tGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGGTTTAGACCAGATAcc > 7:193907/1‑135 (MQ=255) | GAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCGATTGGCAGTTTTAAACCAGATTACCGCTGGTTGACTGAAATGGCTTTGCAAGCAGAACCACTTCAAGAAAGTGGCGGACTCAGCAGGATTCGAACCTGCGACCGCTCGGTTCGTAGCCGAGTACTCTATCCAGCTGAGCTATGAGTCCGCAATTGGCAGTTTTAGACCAGATACC > KT2440/5073123‑5073382 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |