Predicted mutation
evidence seq id position mutation freq annotation gene description
RA KT2440 5,182,333 A→C 15.3% G130G (GGT→GGG)  CJ019_04876 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*KT24405,182,3330AC15.3% 96.2 / 4.4 50G130G (GGT→GGG) CJ019_04876hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (28/13);  new base C (2/7);  total (30/20)
Fisher's exact test for biased strand distribution p-value = 2.07e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCGCTTCTGCAGGATCGAGCCAAAGGTCATGCTGACCAACGCCAGCAGGGCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAA‑CCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATCACCGTGGCCATGACGCCGGGGGTGACATGGGTATCAAGCGCCAACAGATAGAA  >  KT2440/5182203‑5182461
                                                                                                                                  |                                                                                                                                 
tGCGCTTCTGCAGGATCGAGCCAAAGGTCATGCTGACCAACGCCAGCAGGGCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAA‑ccc                                                                                                                               <  6:179804/134‑1 (MQ=255)
   gcTTCTGCAGGATCGAGCCAAAGGTCATGCTGACCAACGCCAGCAGGGCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAA‑CCCAGGc                                                                                                                           >  1:201485/1‑135 (MQ=255)
   gcTTCTGCAGGATCGAGCCAAAGGTCATGCTGACCAACGCCAGCAGGGCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAA‑CCCAGGc                                                                                                                           >  3:305660/1‑135 (MQ=255)
      tCTGCAGGATCGAGCCAAAGGTCATGCTGACCAACGCCAGCAGGGCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGCCCACCATGATCAAGCCGCCCAACCCCAAA‑ACCAGGCCGa                                                                                                                        >  8:123601/1‑135 (MQ=255)
          cAGGATCGAGCCAAAGGTCATGCTGACCAACGCCAGCAGGGCGAACAGCATGCCCGCCAGCGATCCCCCGCCCAGGTTGATGCCCTGGTAGACCCCCATGATCAAGCCGCCCAACCCCAAA‑CCCAGGCCGAACaa                                                                                                                    >  6:23803/1‑135 (MQ=255)
          cAGGATCGAGCCAAAGGTCATGCTGACCAACGCCAGCAGGGCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAC‑CCCAGGCCGAACaa                                                                                                                    >  7:272075/1‑135 (MQ=255)
           aGGATCGAGCCAAAGGTCATGCTGACCAACGCCAGCAGGGCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAA‑CCCAGGCCGAACa                                                                                                                     <  6:78989/133‑1 (MQ=255)
                 gAGCCAAAGGTCATGCTGACCAACGCCAGCAGGGCTAACAGCATGCCCGCCAGCGATACCCCGCCCTGTTTTATGCCCTGGTAGACCACCATCATCAAGCCGCCCAACCCCAAA‑CCCAGGCCGAACAAACGGCTc                                                                                                             <  6:97152/135‑1 (MQ=255)
                  aGCCAAAGGTCATGCTGACCAACGCCAGCAGGGCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCACCCCCACA‑CCCCGCCGGACCAAACGGCGcc                                                                                                            >  4:46339/1‑135 (MQ=255)
                  aGCCAAAGGTCATGCTGACCAACGCCAGCAGGGCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAA‑CCCAGGCCGAACAAACGGCt                                                                                                              >  6:15751/1‑133 (MQ=255)
                  aGCCAAAGGTCATGCTGACCAACGCCAGCAGGGCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCT‑GTAGACCACCATGATCAAGCCGCCCAACCCCAAA‑CCCAGGCCGAACAAACGGCTCCa                                                                                                           >  2:367438/1‑135 (MQ=255)
                     cAAAGGTCATGCTGACCAACGCCAGCAGGGCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGCCCACCATGATCAAGCCGCCCAACCCCAAA‑CCCAGGCCGAACAAACGGCACCAgg                                                                                                         >  6:60759/1‑135 (MQ=255)
                     cAAAGGTCATGCTGACCAACGCCAGCAGGGCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCCAC‑CCCAGGCCGAACAAACGGCTCCAgg                                                                                                         >  8:358537/1‑135 (MQ=255)
                     cAAAGGTCATGCTGACCAACGCCAGCAGGGCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAA‑CCCAGGCCGAACAAACGGCTCCa                                                                                                           >  6:7832/1‑133 (MQ=255)
                                cTGACCAACGCCAGCAGGGCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAA‑CCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGt                                                                                              >  1:75383/1‑135 (MQ=255)
                                      aaCGCCAGCAGGGCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAGCCCCAAA‑CCCAGGCCGAACAACCGGCTCCAGGAGCGCTGGCGTTCCa                                                                                          >  1:6602/1‑133 (MQ=255)
                                      aaCGCCAGCAGGGCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCCAA‑CCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCa                                                                                          >  2:233285/1‑133 (MQ=255)
                                      aaCGCCAGCAGGGCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAA‑CCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCa                                                                                          >  5:85008/1‑133 (MQ=255)
                                        cgcccgccGGGCCAACCCCAGGCCCGCCCGCCAACCCCCGCCCGGGTTGATGCCCGGGAACACCACCATGATCAAGCCGCCCAACCCCAAC‑CCCAGGCCCAACACACGGCTCCAGGAGCGCTGGCGTTCCATCAg                                                                                      <  5:317808/135‑1 (MQ=255)
                                        cGCCAGCTGGGCTAACAGCAGGCCCGCCAGCGATACCCCGCCCCGGTTGAGCCCGTGGTACACCACCATCAACACGCCCCCCAACCCCAAA‑CCCAGCCCTAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAg                                                                                      <  2:75383/135‑1 (MQ=255)
                                                    gAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAA‑CCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAg                                                                          <  6:85008/135‑1 (MQ=255)
                                                    gAACAGCAAGCCCGCCAGCCAACCCCCGCCCAGGTAGATGCCCTGGTAGACCACCTTGATCAACCCGCCCAACCCCAAA‑CCCAGGCCGCACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAg                                                                          <  7:283392/135‑1 (MQ=255)
                                                    caaCCGCAGGCCCGCCAGCGATACCCCGCCCAGGTGGATGCCCTGGTACACCACCATGATCACGCCGCCCACCCCAAAA‑CCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAg                                                                          <  5:289783/134‑1 (MQ=255)
                                                          cATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGAGCAAGCCGCCCAACCCAAAA‑CCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCgg                                                                    <  7:48516/135‑1 (MQ=255)
                                                           cggcccgcccGCCACCCCCCGCCCCGGTGGCAGCCCGGGTCCCCCACCCTGCCCACCCCGCCCACCCCCACC‑CCCCGCCCCAACACACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGt                                                                   <  5:23803/133‑1 (MQ=255)
                                                             gCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCACGCCGCCCACCCCCAAA‑CCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTg                                                                 <  4:339853/135‑1 (MQ=255)
                                                                cGCCAGCGACCCCCCCCCCCGGTAGAGCCCCTGGTACCCCCCCATGACCCCCCCGCCCCACCCAAAC‑CCCCGCCCCACCCCACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTAc                                                              <  5:273753/135‑1 (MQ=255)
                                                                       gATACCCCGCCCAGGTTGATGCCCTGGTAGCCCACCATGATCAAGCCGCCCAACCCCAAA‑CCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATc                                                       >  7:11106/1‑135 (MQ=255)
                                                                       gATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAA‑CCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATc                                                       >  7:274041/1‑135 (MQ=255)
                                                                               gCCCCGGTTGCTGCCCTGGGAGACCACCATGCTCAAGCCGCCCAACCCCAAC‑CCCAGCCCGAACAAACGGCTCCAGGAGCGCGGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATCACCGTGGc                                               <  8:171903/135‑1 (MQ=255)
                                                                                   aGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAA‑CCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACg                                                                               >  4:298656/1‑99 (MQ=255)
                                                                                   aGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAA‑CCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACg                                                                               <  3:298656/99‑1 (MQ=255)
                                                                                   aGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAA‑CCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATCACCGTGGCCATg                                           >  4:304131/1‑135 (MQ=255)
                                                                                   aGGTTGATGCCCTGGTAGACCACCATGATCAAGACGCCCAACCCCACA‑CCCAGGCCGAACAACCGGCTCCAGGAGCGCTGGCGGTCCATCAGCACCACGGTGAGCATCGGTTGTACCCCCATCACCGTGGCCATg                                           >  4:29875/1‑135 (MQ=255)
                                                                                   aGGTTGATGCCCTGGTAGACCACCATGATCAAGACGCCCAACCCCAAA‑CCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACAGTGAGGATCGGTTGTACACCCATCACCGTGGCCATg                                           >  3:349/1‑135 (MQ=255)
                                                                                           gCCCTGGTACACCACCATGAACACCCCCCCCAACCCCAAC‑CCCCGCCCCAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATCACCGTGGCCATGACGCCggg                                   <  7:19785/135‑1 (MQ=255)
                                                                                                          caTGATCAAGCCTCCCACCCCCAAA‑CCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGAACCACGGTGAGGATCGGTTGTACCCCCATCACCGTGGCCATGACGCCGGCGGTGCCATGGGTaga                    >  4:225566/1‑133 (MQ=255)
                                                                                                          caTGATCAAGCCGCCCAACCCCAAA‑CCCAGGCCGAACAACCGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCCTCACCGTGGCCATGACGCCGGGGGTGACATGGGTATc                    >  5:34321/1‑135 (MQ=255)
                                                                                                          caTGATCAAGCCGCCCAACCCCAAA‑CCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATCACCGTGGCCATGACGCCGGGGGTGACATGGGTAt                     >  2:230496/1‑134 (MQ=255)
                                                                                                            tGATCAAGCCGCCCAACCCCAAA‑CCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATCACCGAGGCCATGACGCCGGGGGTGACATGGGTATCaa                  >  5:80314/1‑135 (MQ=255)
                                                                                                             gaccAAGCCCCCCACCCCCAAC‑CCCCGCCCGACCACACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATCACCGTGGCCATGACGCCGGGGGTGACATGGGTATCAAg                 <  1:233285/132‑1 (MQ=255)
                                                                                                              aTCAAGCCGCCCAACCACAAA‑AAAAGTAAGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACCGTGAGGATCTGTTGTACCCCCATCACCGTGGCCATGACGCCGGGGGTGACATGGGTATCAAgc                >  3:108518/1‑135 (MQ=255)
                                                                                                                       cccACCCCCAAA‑CCCCGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCCGCACCACGGGGAGGATCGGTTGTACCCCCATCACCGTGGCCATGACGCCGGGGGTGACATGGGTATCAAGCGCCAACAGa       <  8:287057/135‑1 (MQ=255)
                                                                                                                       cccAACCCCAAA‑CCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATCACCGTGGCCATGACGCCGGGGGTGACATGGGTATCAAGCGCCAACAg        >  3:334559/1‑134 (MQ=255)
                                                                                                                         ccaCCCCAAACCCCCGCCCCAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATCACCGTGGCCATGACGCCGGGGGTGACATGGGTATCAAGCGCCAACAGAt      <  5:7832/133‑1 (MQ=255)
                                                                                                                          aaCCCCAAA‑CCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATCACCGTGGCCATGACGCCGGGGGTGACATGGGTATCAAGCGCCAACAGATa     >  3:27694/1‑134 (MQ=255)
                                                                                                                          aaCCCCAAA‑CCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATCACCGTGGCCATGACGCCGGGGGTGACATGGGTATCAAGCGCCAACAGATa     >  1:361150/1‑134 (MQ=255)
                                                                                                                          aaCCACAAA‑CCAAGGCCGAAAAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGATGATCGGTTGTACCCCCATCACCGTGGCCATGACGCCGGGGGTGACATGGTAATCAATCGCCAACAGATa     >  2:88312/1‑134 (MQ=255)
                                                                                                                            ccccAAC‑CCCCGCCCCAACACACGGCTCCAGGCGCGCTGGCGTTCCATCACCACCACGGGGAGGATCGGTTGTACCCCCATCACCGTGGCCATGACGCCGGGGGTGACATGGGTATCAAGCGCCAACAGATagaa  <  5:15751/135‑1 (MQ=255)
                                                                                                                            ccccAAA‑CCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCAATCAGCACCACGGTGAGGATCGGTTGTACCCCCATCACCGTGGCCATGACGCCGGGGGTGACATGGGTATCAAGCGCCAACAGATagaa  <  3:304131/135‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                 
TGCGCTTCTGCAGGATCGAGCCAAAGGTCATGCTGACCAACGCCAGCAGGGCGAACAGCATGCCCGCCAGCGATACCCCGCCCAGGTTGATGCCCTGGTAGACCACCATGATCAAGCCGCCCAACCCCAAA‑CCCAGGCCGAACAAACGGCTCCAGGAGCGCTGGCGTTCCATCAGCACCACGGTGAGGATCGGTTGTACCCCCATCACCGTGGCCATGACGCCGGGGGTGACATGGGTATCAAGCGCCAACAGATAGAA  >  KT2440/5182203‑5182461

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑